TRIAMF: A New Method for Delivery of Cas9 Ribonucleoprotein Complex to Human Hematopoietic Stem Cells View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-12

AUTHORS

Jonathan Yen, Michael Fiorino, Yi Liu, Steve Paula, Scott Clarkson, Lisa Quinn, William R. Tschantz, Heath Klock, Ning Guo, Carsten Russ, Vionnie W. C. Yu, Craig Mickanin, Susan C. Stevenson, Cameron Lee, Yi Yang

ABSTRACT

CRISPR/Cas9 mediated gene editing of patient-derived hematopoietic stem and progenitor cells (HSPCs) ex vivo followed by autologous transplantation of the edited HSPCs back to the patient can provide a potential cure for monogenic blood disorders such as β-hemoglobinopathies. One challenge for this strategy is efficient delivery of the ribonucleoprotein (RNP) complex, consisting of purified Cas9 protein and guide RNA, into HSPCs. Because β-hemoglobinopathies are most prevalent in developing countries, it is desirable to have a reliable, efficient, easy-to-use and cost effective delivery method. With this goal in mind, we developed TRansmembrane Internalization Assisted by Membrane Filtration (TRIAMF), a new method to quickly and effectively deliver RNPs into HSPCs by passing a RNP and cell mixture through a filter membrane. We achieved robust gene editing in HSPCs using TRIAMF and demonstrated that the multilineage colony forming capacities and the competence for engraftment in immunocompromised mice of HSPCs were preserved post TRIAMF treatment. TRIAMF is a custom designed system using inexpensive components and has the capacity to process HSPCs at clinical scale. More... »

PAGES

16304

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-018-34601-6

DOI

http://dx.doi.org/10.1038/s41598-018-34601-6

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1107913010

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30389991


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Novartis (United States)", 
          "id": "https://www.grid.ac/institutes/grid.418424.f", 
          "name": [
            "Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yen", 
        "givenName": "Jonathan", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Novartis (United States)", 
          "id": "https://www.grid.ac/institutes/grid.418424.f", 
          "name": [
            "NIBR Informatics, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fiorino", 
        "givenName": "Michael", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Novartis (United States)", 
          "id": "https://www.grid.ac/institutes/grid.418424.f", 
          "name": [
            "Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Liu", 
        "givenName": "Yi", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Novartis (United States)", 
          "id": "https://www.grid.ac/institutes/grid.418424.f", 
          "name": [
            "Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Paula", 
        "givenName": "Steve", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Novartis (United States)", 
          "id": "https://www.grid.ac/institutes/grid.418424.f", 
          "name": [
            "Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Clarkson", 
        "givenName": "Scott", 
        "id": "sg:person.013257601554.09", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013257601554.09"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Novartis (United States)", 
          "id": "https://www.grid.ac/institutes/grid.418424.f", 
          "name": [
            "Biotherapeutic and Analytical Tech, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Quinn", 
        "givenName": "Lisa", 
        "id": "sg:person.0611306524.09", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0611306524.09"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Novartis (United States)", 
          "id": "https://www.grid.ac/institutes/grid.418424.f", 
          "name": [
            "Biotherapeutic and Analytical Tech, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tschantz", 
        "givenName": "William R.", 
        "id": "sg:person.01000137675.55", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01000137675.55"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Genomics Institute of the Novartis Research Foundation", 
          "id": "https://www.grid.ac/institutes/grid.418185.1", 
          "name": [
            "Biotherapeutics & Biotechnology, The Genomics Institute of the Novartis Research Foundation, La Jolla, California, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Klock", 
        "givenName": "Heath", 
        "id": "sg:person.01357335165.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01357335165.07"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Novartis (United States)", 
          "id": "https://www.grid.ac/institutes/grid.418424.f", 
          "name": [
            "Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Guo", 
        "givenName": "Ning", 
        "id": "sg:person.01340461776.40", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01340461776.40"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Novartis (United States)", 
          "id": "https://www.grid.ac/institutes/grid.418424.f", 
          "name": [
            "Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Russ", 
        "givenName": "Carsten", 
        "id": "sg:person.01300612454.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300612454.35"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Novartis (United States)", 
          "id": "https://www.grid.ac/institutes/grid.418424.f", 
          "name": [
            "Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yu", 
        "givenName": "Vionnie W. C.", 
        "id": "sg:person.016277546132.50", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016277546132.50"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Novartis (United States)", 
          "id": "https://www.grid.ac/institutes/grid.418424.f", 
          "name": [
            "Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mickanin", 
        "givenName": "Craig", 
        "id": "sg:person.01206570261.21", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01206570261.21"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Novartis (United States)", 
          "id": "https://www.grid.ac/institutes/grid.418424.f", 
          "name": [
            "Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Stevenson", 
        "givenName": "Susan C.", 
        "id": "sg:person.0663711275.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0663711275.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Novartis (United States)", 
          "id": "https://www.grid.ac/institutes/grid.418424.f", 
          "name": [
            "Global Discovery Chemistry, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lee", 
        "givenName": "Cameron", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Novartis (United States)", 
          "id": "https://www.grid.ac/institutes/grid.418424.f", 
          "name": [
            "Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yang", 
        "givenName": "Yi", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1155/2012/507894", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000599805"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt.3290", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001100614", 
          "https://doi.org/10.1038/nbt.3290"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nprot.2013.118", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001476968", 
          "https://doi.org/10.1038/nprot.2013.118"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature19764", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002288746", 
          "https://doi.org/10.1038/nature19764"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncomms8085", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004816533", 
          "https://doi.org/10.1038/ncomms8085"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/29.1.308", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005817660"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1218705110", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008514650"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/hum.2016.009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008773313"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nri1779", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010614752", 
          "https://doi.org/10.1038/nri1779"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nri1779", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010614752", 
          "https://doi.org/10.1038/nri1779"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.89.7.2804", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010787745"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2015.03.028", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012478310"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2015.03.028", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012478310"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.185.1.111", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012827630"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/blood-2009-01-201863", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013029287"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci87885", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015935133"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt.3081", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017697957", 
          "https://doi.org/10.1038/nbt.3081"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2016.06.037", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019385127"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gr.171264.113", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019923892"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkw227", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021051016"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1520244113", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021973102"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/blood-2002-03-0898", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022491603"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2014.05.050", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023033599"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gr.171322.113", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024649491"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm.4170", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027882655", 
          "https://doi.org/10.1038/nm.4170"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/b916505n", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029528459"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/b916505n", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029528459"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/anie.201506030", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030802081"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature15521", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032459584", 
          "https://doi.org/10.1038/nature15521"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.celrep.2016.09.092", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033985766"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.celrep.2016.09.092", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033985766"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bit.21651", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034079635"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jbiotec.2015.04.024", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036283241"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejm199602013340501", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036318792"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/blood-2010-08-300723", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037729963"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/bioinformatics/btp324", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038266369"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature20134", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038845840", 
          "https://doi.org/10.1038/nature20134"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/anie.201610209", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040201704"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1225829", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041850060"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/sciadv.1500454", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045291486"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.177.5.1331", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045898094"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.omtm.2016.12.009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049047651"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pmed.1001484", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049509452"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.stem.2014.10.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049547074"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.stem.2014.10.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049547074"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1097-0290(19991105)65:3<341::aid-bit12>3.0.co;2-i", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052456505"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c3ib40215k", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052531888"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.283.5403.845", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062564103"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074645474", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jconrel.2017.02.007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083541084"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt.3806", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083804751", 
          "https://doi.org/10.1038/nbt.3806"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4084/mjhid.2017.015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083998414"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ymeth.2017.04.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084749578"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ymeth.2017.04.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084749578"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejmra1510865", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084972366"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/14651858.cd002202.pub2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084988208"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c7ib00060j", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085105929"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.21769/bioprotoc.2586", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1092367085"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/bjh.15038", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1093059275"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2018-12", 
    "datePublishedReg": "2018-12-01", 
    "description": "CRISPR/Cas9 mediated gene editing of patient-derived hematopoietic stem and progenitor cells (HSPCs) ex vivo followed by autologous transplantation of the edited HSPCs back to the patient can provide a potential cure for monogenic blood disorders such as \u03b2-hemoglobinopathies. One challenge for this strategy is efficient delivery of the ribonucleoprotein (RNP) complex, consisting of purified Cas9 protein and guide RNA, into HSPCs. Because \u03b2-hemoglobinopathies are most prevalent in developing countries, it is desirable to have a reliable, efficient, easy-to-use and cost effective delivery method. With this goal in mind, we developed TRansmembrane Internalization Assisted by Membrane Filtration (TRIAMF), a new method to quickly and effectively deliver RNPs into HSPCs by passing a RNP and cell mixture through a filter membrane. We achieved robust gene editing in HSPCs using TRIAMF and demonstrated that the multilineage colony forming capacities and the competence for engraftment in immunocompromised mice of HSPCs were preserved post TRIAMF treatment. TRIAMF is a custom designed system using inexpensive components and has the capacity to process HSPCs at clinical scale.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/s41598-018-34601-6", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1045337", 
        "issn": [
          "2045-2322"
        ], 
        "name": "Scientific Reports", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "8"
      }
    ], 
    "name": "TRIAMF: A New Method for Delivery of Cas9 Ribonucleoprotein Complex to Human Hematopoietic Stem Cells", 
    "pagination": "16304", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "3927bb336615b61726115020733fd6feccb060e4f4f1fcfd140236e5b4fa6ce6"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30389991"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101563288"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/s41598-018-34601-6"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1107913010"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/s41598-018-34601-6", 
      "https://app.dimensions.ai/details/publication/pub.1107913010"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T22:49", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8690_00000610.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/s41598-018-34601-6"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-34601-6'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-34601-6'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-34601-6'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-34601-6'


 

This table displays all metadata directly associated to this object as RDF triples.

334 TRIPLES      21 PREDICATES      82 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/s41598-018-34601-6 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N8fc6744dd6d240e2ab2230ae35e25769
4 schema:citation sg:pub.10.1038/nature15521
5 sg:pub.10.1038/nature19764
6 sg:pub.10.1038/nature20134
7 sg:pub.10.1038/nbt.3081
8 sg:pub.10.1038/nbt.3290
9 sg:pub.10.1038/nbt.3806
10 sg:pub.10.1038/ncomms8085
11 sg:pub.10.1038/nm.4170
12 sg:pub.10.1038/nprot.2013.118
13 sg:pub.10.1038/nri1779
14 https://app.dimensions.ai/details/publication/pub.1074645474
15 https://doi.org/10.1002/(sici)1097-0290(19991105)65:3<341::aid-bit12>3.0.co;2-i
16 https://doi.org/10.1002/14651858.cd002202.pub2
17 https://doi.org/10.1002/anie.201506030
18 https://doi.org/10.1002/anie.201610209
19 https://doi.org/10.1002/bit.21651
20 https://doi.org/10.1016/j.cell.2014.05.050
21 https://doi.org/10.1016/j.cell.2015.03.028
22 https://doi.org/10.1016/j.celrep.2016.09.092
23 https://doi.org/10.1016/j.jbiotec.2015.04.024
24 https://doi.org/10.1016/j.jconrel.2017.02.007
25 https://doi.org/10.1016/j.molcel.2016.06.037
26 https://doi.org/10.1016/j.omtm.2016.12.009
27 https://doi.org/10.1016/j.stem.2014.10.004
28 https://doi.org/10.1016/j.ymeth.2017.04.003
29 https://doi.org/10.1039/b916505n
30 https://doi.org/10.1039/c3ib40215k
31 https://doi.org/10.1039/c7ib00060j
32 https://doi.org/10.1056/nejm199602013340501
33 https://doi.org/10.1056/nejmra1510865
34 https://doi.org/10.1073/pnas.1218705110
35 https://doi.org/10.1073/pnas.1520244113
36 https://doi.org/10.1073/pnas.89.7.2804
37 https://doi.org/10.1084/jem.177.5.1331
38 https://doi.org/10.1084/jem.185.1.111
39 https://doi.org/10.1089/hum.2016.009
40 https://doi.org/10.1093/bioinformatics/btp324
41 https://doi.org/10.1093/nar/29.1.308
42 https://doi.org/10.1093/nar/gkw227
43 https://doi.org/10.1101/gr.171264.113
44 https://doi.org/10.1101/gr.171322.113
45 https://doi.org/10.1111/bjh.15038
46 https://doi.org/10.1126/sciadv.1500454
47 https://doi.org/10.1126/science.1225829
48 https://doi.org/10.1126/science.283.5403.845
49 https://doi.org/10.1155/2012/507894
50 https://doi.org/10.1172/jci87885
51 https://doi.org/10.1182/blood-2002-03-0898
52 https://doi.org/10.1182/blood-2009-01-201863
53 https://doi.org/10.1182/blood-2010-08-300723
54 https://doi.org/10.1371/journal.pmed.1001484
55 https://doi.org/10.21769/bioprotoc.2586
56 https://doi.org/10.4084/mjhid.2017.015
57 schema:datePublished 2018-12
58 schema:datePublishedReg 2018-12-01
59 schema:description CRISPR/Cas9 mediated gene editing of patient-derived hematopoietic stem and progenitor cells (HSPCs) ex vivo followed by autologous transplantation of the edited HSPCs back to the patient can provide a potential cure for monogenic blood disorders such as β-hemoglobinopathies. One challenge for this strategy is efficient delivery of the ribonucleoprotein (RNP) complex, consisting of purified Cas9 protein and guide RNA, into HSPCs. Because β-hemoglobinopathies are most prevalent in developing countries, it is desirable to have a reliable, efficient, easy-to-use and cost effective delivery method. With this goal in mind, we developed TRansmembrane Internalization Assisted by Membrane Filtration (TRIAMF), a new method to quickly and effectively deliver RNPs into HSPCs by passing a RNP and cell mixture through a filter membrane. We achieved robust gene editing in HSPCs using TRIAMF and demonstrated that the multilineage colony forming capacities and the competence for engraftment in immunocompromised mice of HSPCs were preserved post TRIAMF treatment. TRIAMF is a custom designed system using inexpensive components and has the capacity to process HSPCs at clinical scale.
60 schema:genre research_article
61 schema:inLanguage en
62 schema:isAccessibleForFree true
63 schema:isPartOf Nbfca4cad07274a028678330f706f36ea
64 Ndc14f61d0afa401e92222c2d876f57e9
65 sg:journal.1045337
66 schema:name TRIAMF: A New Method for Delivery of Cas9 Ribonucleoprotein Complex to Human Hematopoietic Stem Cells
67 schema:pagination 16304
68 schema:productId N214262885755463db073b28bcb9cfc9c
69 N51931216597d409695b96ef795fa7bb8
70 Nb08f96457b0642e694fa43c511ebc71d
71 Nb860d5f9638843abbb46dc4b43e12596
72 Ne40641dc92ba4630b6f0263052cb6317
73 schema:sameAs https://app.dimensions.ai/details/publication/pub.1107913010
74 https://doi.org/10.1038/s41598-018-34601-6
75 schema:sdDatePublished 2019-04-10T22:49
76 schema:sdLicense https://scigraph.springernature.com/explorer/license/
77 schema:sdPublisher Naaed734976d241f892a4a74da2b0f7b1
78 schema:url https://www.nature.com/articles/s41598-018-34601-6
79 sgo:license sg:explorer/license/
80 sgo:sdDataset articles
81 rdf:type schema:ScholarlyArticle
82 N0b2086036e8a44959ae12d94daf96fac rdf:first sg:person.0663711275.06
83 rdf:rest N87c843d83ecb4236b2b3af5fde39d5d6
84 N117b2e68b6f848b6bbb986e606715ddf rdf:first sg:person.01340461776.40
85 rdf:rest Ncf1353c45cc1465f984c52d317adbdcf
86 N214262885755463db073b28bcb9cfc9c schema:name readcube_id
87 schema:value 3927bb336615b61726115020733fd6feccb060e4f4f1fcfd140236e5b4fa6ce6
88 rdf:type schema:PropertyValue
89 N25fa47e1537e4ad286cd811d0ff6abd2 schema:affiliation https://www.grid.ac/institutes/grid.418424.f
90 schema:familyName Lee
91 schema:givenName Cameron
92 rdf:type schema:Person
93 N33731149955a415e96043f3815afca30 schema:affiliation https://www.grid.ac/institutes/grid.418424.f
94 schema:familyName Yen
95 schema:givenName Jonathan
96 rdf:type schema:Person
97 N48707bc932934eeb99e9467b5090adad rdf:first Ncf7d2f6f0c0748bd98e3b9a0221369c0
98 rdf:rest N72c01922030d41cdb39a02ad989fa182
99 N51931216597d409695b96ef795fa7bb8 schema:name doi
100 schema:value 10.1038/s41598-018-34601-6
101 rdf:type schema:PropertyValue
102 N52bae7b3fce64230903ae15209b8b788 rdf:first sg:person.013257601554.09
103 rdf:rest N95f67d390e9a4d7382937416f8939c79
104 N6542873878934bba9a5c35bf19aa4842 rdf:first sg:person.016277546132.50
105 rdf:rest N7729d7417a054fa4b67ae9ad1ba2dd40
106 N668e21cd6c2d48c3b71330df3fd3c874 schema:affiliation https://www.grid.ac/institutes/grid.418424.f
107 schema:familyName Yang
108 schema:givenName Yi
109 rdf:type schema:Person
110 N72c01922030d41cdb39a02ad989fa182 rdf:first N90935c7afd1244ce92c64c318193df5e
111 rdf:rest N52bae7b3fce64230903ae15209b8b788
112 N7729d7417a054fa4b67ae9ad1ba2dd40 rdf:first sg:person.01206570261.21
113 rdf:rest N0b2086036e8a44959ae12d94daf96fac
114 N87c843d83ecb4236b2b3af5fde39d5d6 rdf:first N25fa47e1537e4ad286cd811d0ff6abd2
115 rdf:rest Nce8de56f449b4c5898baf6d7c352a76a
116 N8fc6744dd6d240e2ab2230ae35e25769 rdf:first N33731149955a415e96043f3815afca30
117 rdf:rest Naa8ce644d6a745029eca2b8de81d21db
118 N90935c7afd1244ce92c64c318193df5e schema:affiliation https://www.grid.ac/institutes/grid.418424.f
119 schema:familyName Paula
120 schema:givenName Steve
121 rdf:type schema:Person
122 N95f67d390e9a4d7382937416f8939c79 rdf:first sg:person.0611306524.09
123 rdf:rest Nde50d42bc6b4468a9376257346486986
124 N9caba42c34304f7a8d2bc8bf37450742 schema:affiliation https://www.grid.ac/institutes/grid.418424.f
125 schema:familyName Fiorino
126 schema:givenName Michael
127 rdf:type schema:Person
128 Naa8ce644d6a745029eca2b8de81d21db rdf:first N9caba42c34304f7a8d2bc8bf37450742
129 rdf:rest N48707bc932934eeb99e9467b5090adad
130 Naaed734976d241f892a4a74da2b0f7b1 schema:name Springer Nature - SN SciGraph project
131 rdf:type schema:Organization
132 Naf3f8d00741c4f22bc51e9c09af604f6 rdf:first sg:person.01357335165.07
133 rdf:rest N117b2e68b6f848b6bbb986e606715ddf
134 Nb08f96457b0642e694fa43c511ebc71d schema:name nlm_unique_id
135 schema:value 101563288
136 rdf:type schema:PropertyValue
137 Nb860d5f9638843abbb46dc4b43e12596 schema:name dimensions_id
138 schema:value pub.1107913010
139 rdf:type schema:PropertyValue
140 Nbfca4cad07274a028678330f706f36ea schema:issueNumber 1
141 rdf:type schema:PublicationIssue
142 Nce8de56f449b4c5898baf6d7c352a76a rdf:first N668e21cd6c2d48c3b71330df3fd3c874
143 rdf:rest rdf:nil
144 Ncf1353c45cc1465f984c52d317adbdcf rdf:first sg:person.01300612454.35
145 rdf:rest N6542873878934bba9a5c35bf19aa4842
146 Ncf7d2f6f0c0748bd98e3b9a0221369c0 schema:affiliation https://www.grid.ac/institutes/grid.418424.f
147 schema:familyName Liu
148 schema:givenName Yi
149 rdf:type schema:Person
150 Ndc14f61d0afa401e92222c2d876f57e9 schema:volumeNumber 8
151 rdf:type schema:PublicationVolume
152 Nde50d42bc6b4468a9376257346486986 rdf:first sg:person.01000137675.55
153 rdf:rest Naf3f8d00741c4f22bc51e9c09af604f6
154 Ne40641dc92ba4630b6f0263052cb6317 schema:name pubmed_id
155 schema:value 30389991
156 rdf:type schema:PropertyValue
157 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
158 schema:name Biological Sciences
159 rdf:type schema:DefinedTerm
160 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
161 schema:name Biochemistry and Cell Biology
162 rdf:type schema:DefinedTerm
163 sg:journal.1045337 schema:issn 2045-2322
164 schema:name Scientific Reports
165 rdf:type schema:Periodical
166 sg:person.01000137675.55 schema:affiliation https://www.grid.ac/institutes/grid.418424.f
167 schema:familyName Tschantz
168 schema:givenName William R.
169 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01000137675.55
170 rdf:type schema:Person
171 sg:person.01206570261.21 schema:affiliation https://www.grid.ac/institutes/grid.418424.f
172 schema:familyName Mickanin
173 schema:givenName Craig
174 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01206570261.21
175 rdf:type schema:Person
176 sg:person.01300612454.35 schema:affiliation https://www.grid.ac/institutes/grid.418424.f
177 schema:familyName Russ
178 schema:givenName Carsten
179 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300612454.35
180 rdf:type schema:Person
181 sg:person.013257601554.09 schema:affiliation https://www.grid.ac/institutes/grid.418424.f
182 schema:familyName Clarkson
183 schema:givenName Scott
184 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013257601554.09
185 rdf:type schema:Person
186 sg:person.01340461776.40 schema:affiliation https://www.grid.ac/institutes/grid.418424.f
187 schema:familyName Guo
188 schema:givenName Ning
189 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01340461776.40
190 rdf:type schema:Person
191 sg:person.01357335165.07 schema:affiliation https://www.grid.ac/institutes/grid.418185.1
192 schema:familyName Klock
193 schema:givenName Heath
194 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01357335165.07
195 rdf:type schema:Person
196 sg:person.016277546132.50 schema:affiliation https://www.grid.ac/institutes/grid.418424.f
197 schema:familyName Yu
198 schema:givenName Vionnie W. C.
199 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016277546132.50
200 rdf:type schema:Person
201 sg:person.0611306524.09 schema:affiliation https://www.grid.ac/institutes/grid.418424.f
202 schema:familyName Quinn
203 schema:givenName Lisa
204 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0611306524.09
205 rdf:type schema:Person
206 sg:person.0663711275.06 schema:affiliation https://www.grid.ac/institutes/grid.418424.f
207 schema:familyName Stevenson
208 schema:givenName Susan C.
209 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0663711275.06
210 rdf:type schema:Person
211 sg:pub.10.1038/nature15521 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032459584
212 https://doi.org/10.1038/nature15521
213 rdf:type schema:CreativeWork
214 sg:pub.10.1038/nature19764 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002288746
215 https://doi.org/10.1038/nature19764
216 rdf:type schema:CreativeWork
217 sg:pub.10.1038/nature20134 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038845840
218 https://doi.org/10.1038/nature20134
219 rdf:type schema:CreativeWork
220 sg:pub.10.1038/nbt.3081 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017697957
221 https://doi.org/10.1038/nbt.3081
222 rdf:type schema:CreativeWork
223 sg:pub.10.1038/nbt.3290 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001100614
224 https://doi.org/10.1038/nbt.3290
225 rdf:type schema:CreativeWork
226 sg:pub.10.1038/nbt.3806 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083804751
227 https://doi.org/10.1038/nbt.3806
228 rdf:type schema:CreativeWork
229 sg:pub.10.1038/ncomms8085 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004816533
230 https://doi.org/10.1038/ncomms8085
231 rdf:type schema:CreativeWork
232 sg:pub.10.1038/nm.4170 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027882655
233 https://doi.org/10.1038/nm.4170
234 rdf:type schema:CreativeWork
235 sg:pub.10.1038/nprot.2013.118 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001476968
236 https://doi.org/10.1038/nprot.2013.118
237 rdf:type schema:CreativeWork
238 sg:pub.10.1038/nri1779 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010614752
239 https://doi.org/10.1038/nri1779
240 rdf:type schema:CreativeWork
241 https://app.dimensions.ai/details/publication/pub.1074645474 schema:CreativeWork
242 https://doi.org/10.1002/(sici)1097-0290(19991105)65:3<341::aid-bit12>3.0.co;2-i schema:sameAs https://app.dimensions.ai/details/publication/pub.1052456505
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1002/14651858.cd002202.pub2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084988208
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1002/anie.201506030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030802081
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1002/anie.201610209 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040201704
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1002/bit.21651 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034079635
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1016/j.cell.2014.05.050 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023033599
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1016/j.cell.2015.03.028 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012478310
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1016/j.celrep.2016.09.092 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033985766
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1016/j.jbiotec.2015.04.024 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036283241
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1016/j.jconrel.2017.02.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083541084
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1016/j.molcel.2016.06.037 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019385127
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1016/j.omtm.2016.12.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049047651
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1016/j.stem.2014.10.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049547074
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1016/j.ymeth.2017.04.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084749578
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1039/b916505n schema:sameAs https://app.dimensions.ai/details/publication/pub.1029528459
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1039/c3ib40215k schema:sameAs https://app.dimensions.ai/details/publication/pub.1052531888
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1039/c7ib00060j schema:sameAs https://app.dimensions.ai/details/publication/pub.1085105929
275 rdf:type schema:CreativeWork
276 https://doi.org/10.1056/nejm199602013340501 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036318792
277 rdf:type schema:CreativeWork
278 https://doi.org/10.1056/nejmra1510865 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084972366
279 rdf:type schema:CreativeWork
280 https://doi.org/10.1073/pnas.1218705110 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008514650
281 rdf:type schema:CreativeWork
282 https://doi.org/10.1073/pnas.1520244113 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021973102
283 rdf:type schema:CreativeWork
284 https://doi.org/10.1073/pnas.89.7.2804 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010787745
285 rdf:type schema:CreativeWork
286 https://doi.org/10.1084/jem.177.5.1331 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045898094
287 rdf:type schema:CreativeWork
288 https://doi.org/10.1084/jem.185.1.111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012827630
289 rdf:type schema:CreativeWork
290 https://doi.org/10.1089/hum.2016.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008773313
291 rdf:type schema:CreativeWork
292 https://doi.org/10.1093/bioinformatics/btp324 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038266369
293 rdf:type schema:CreativeWork
294 https://doi.org/10.1093/nar/29.1.308 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005817660
295 rdf:type schema:CreativeWork
296 https://doi.org/10.1093/nar/gkw227 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021051016
297 rdf:type schema:CreativeWork
298 https://doi.org/10.1101/gr.171264.113 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019923892
299 rdf:type schema:CreativeWork
300 https://doi.org/10.1101/gr.171322.113 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024649491
301 rdf:type schema:CreativeWork
302 https://doi.org/10.1111/bjh.15038 schema:sameAs https://app.dimensions.ai/details/publication/pub.1093059275
303 rdf:type schema:CreativeWork
304 https://doi.org/10.1126/sciadv.1500454 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045291486
305 rdf:type schema:CreativeWork
306 https://doi.org/10.1126/science.1225829 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041850060
307 rdf:type schema:CreativeWork
308 https://doi.org/10.1126/science.283.5403.845 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062564103
309 rdf:type schema:CreativeWork
310 https://doi.org/10.1155/2012/507894 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000599805
311 rdf:type schema:CreativeWork
312 https://doi.org/10.1172/jci87885 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015935133
313 rdf:type schema:CreativeWork
314 https://doi.org/10.1182/blood-2002-03-0898 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022491603
315 rdf:type schema:CreativeWork
316 https://doi.org/10.1182/blood-2009-01-201863 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013029287
317 rdf:type schema:CreativeWork
318 https://doi.org/10.1182/blood-2010-08-300723 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037729963
319 rdf:type schema:CreativeWork
320 https://doi.org/10.1371/journal.pmed.1001484 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049509452
321 rdf:type schema:CreativeWork
322 https://doi.org/10.21769/bioprotoc.2586 schema:sameAs https://app.dimensions.ai/details/publication/pub.1092367085
323 rdf:type schema:CreativeWork
324 https://doi.org/10.4084/mjhid.2017.015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083998414
325 rdf:type schema:CreativeWork
326 https://www.grid.ac/institutes/grid.418185.1 schema:alternateName Genomics Institute of the Novartis Research Foundation
327 schema:name Biotherapeutics & Biotechnology, The Genomics Institute of the Novartis Research Foundation, La Jolla, California, USA
328 rdf:type schema:Organization
329 https://www.grid.ac/institutes/grid.418424.f schema:alternateName Novartis (United States)
330 schema:name Biotherapeutic and Analytical Tech, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA
331 Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA
332 Global Discovery Chemistry, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA
333 NIBR Informatics, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA
334 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...