Solid-phase enzyme catalysis of DNA end repair and 3′ A-tailing reduces GC-bias in next-generation sequencing of human genomic DNA View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-12

AUTHORS

Aihua Zhang, Shaohua Li, Lynne Apone, Xiaoli Sun, Lixin Chen, Laurence M. Ettwiller, Bradley W. Langhorst, Christopher J. Noren, Ming-Qun Xu

ABSTRACT

The use of next-generation sequencing (NGS) has been instrumental in advancing biological research and clinical diagnostics. To fully utilize the power of NGS, complete, uniform coverage of the entire genome is required. In this study, we identified the primary sources of bias observed in sequence coverage across AT-rich regions of the human genome with existing amplification-free DNA library preparation methods. We have found evidence that a major source of bias is the inefficient processing of AT-rich DNA in end repair and 3' A-tailing, causing under-representation of extremely AT-rich regions. We have employed immobilized DNA modifying enzymes to catalyze end repair and 3' A-tailing reactions, to notably reduce the GC bias observed with existing library construction methods. More... »

PAGES

15887

Journal

TITLE

Scientific Reports

ISSUE

1

VOLUME

8

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-018-34079-2

DOI

http://dx.doi.org/10.1038/s41598-018-34079-2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1107771929

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30367148


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