Bacterial cell wall nanoimaging by autoblinking microscopy View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-12

AUTHORS

Kevin Floc’h, Françoise Lacroix, Liliana Barbieri, Pascale Servant, Remi Galland, Corey Butler, Jean-Baptiste Sibarita, Dominique Bourgeois, Joanna Timmins

ABSTRACT

Spurious blinking fluorescent spots are often seen in bacteria during single-molecule localization microscopy experiments. Although this 'autoblinking' phenomenon is widespread, its origin remains unclear. In Deinococcus strains, we observed particularly strong autoblinking at the periphery of the bacteria, facilitating its comprehensive characterization. A systematic evaluation of the contributions of different components of the sample environment to autoblinking levels and the in-depth analysis of the photophysical properties of autoblinking molecules indicate that the phenomenon results from transient binding of fluorophores originating mostly from the growth medium to the bacterial cell wall, which produces single-molecule fluorescence through a Point Accumulation for Imaging in Nanoscale Topography (PAINT) mechanism. Our data suggest that the autoblinking molecules preferentially bind to the plasma membrane of bacterial cells. Autoblinking microscopy was used to acquire nanoscale images of live, unlabeled D. radiodurans and could be combined with PALM imaging of PAmCherry-labeled bacteria in two-color experiments. Autoblinking-based super-resolved images provided insight into the formation of septa in dividing bacteria and revealed heterogeneities in the distribution and dynamics of autoblinking molecules within the cell wall. More... »

PAGES

14038

References to SciGraph publications

  • 2008-02. High-density mapping of single-molecule trajectories with photoactivated localization microscopy in NATURE METHODS
  • 2014-12. TrackArt: the user friendly interface for single molecule tracking data analysis and simulation applied to complex diffusion in mica supported lipid bilayers in BMC RESEARCH NOTES
  • 2006-10. Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM) in NATURE METHODS
  • 2014-01. Exploring bacterial cell biology with single-molecule tracking and super-resolution imaging in NATURE REVIEWS MICROBIOLOGY
  • 2012-07. Fiji: an open-source platform for biological-image analysis in NATURE METHODS
  • 1965-09. The fine structure of micrococcus radiodurans in ARCHIVES OF MICROBIOLOGY
  • 2013-06. Measuring image resolution in optical nanoscopy in NATURE METHODS
  • 2015-11. SR-Tesseler: a method to segment and quantify localization-based super-resolution microscopy data in NATURE METHODS
  • 2016-08-26. Single-molecule imaging of UvrA and UvrB recruitment to DNA lesions in living Escherichia coli in NATURE COMMUNICATIONS
  • 2017-12. Resonant out-of-phase fluorescence microscopy and remote imaging overcome spectral limitations in NATURE COMMUNICATIONS
  • 2007-10. Knockout of crtB or crtI gene blocks the carotenoid biosynthetic pathway in Deinococcus radiodurans R1 and influences its resistance to oxidative DNA-damaging agents due to change of free radicals scavenging ability in ARCHIVES OF MICROBIOLOGY
  • 2011-02. High versus low level expression of the lycopene biosynthesis genes from Pantoea ananatis in Escherichia coli in BIOTECHNOLOGY LETTERS
  • 2015-10. Ultrahigh-throughput single-molecule spectroscopy and spectrally resolved super-resolution microscopy in NATURE METHODS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41598-018-32335-z

    DOI

    http://dx.doi.org/10.1038/s41598-018-32335-z

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1106971227

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30232348


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Grenoble Alpes University", 
              "id": "https://www.grid.ac/institutes/grid.450307.5", 
              "name": [
                "Univ. Grenoble Alpes, CEA, CNRS, IBS, F-38000, Grenoble, France"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Floc\u2019h", 
            "givenName": "Kevin", 
            "id": "sg:person.012567422720.30", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012567422720.30"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Grenoble Alpes University", 
              "id": "https://www.grid.ac/institutes/grid.450307.5", 
              "name": [
                "Univ. Grenoble Alpes, CEA, CNRS, IBS, F-38000, Grenoble, France"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lacroix", 
            "givenName": "Fran\u00e7oise", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Grenoble Alpes University", 
              "id": "https://www.grid.ac/institutes/grid.450307.5", 
              "name": [
                "Univ. Grenoble Alpes, CEA, CNRS, IBS, F-38000, Grenoble, France"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Barbieri", 
            "givenName": "Liliana", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institute of Integrative Biology of the Cell", 
              "id": "https://www.grid.ac/institutes/grid.462411.4", 
              "name": [
                "Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Universit\u00e9 Paris-Saclay, 91198 Gif-sur-Yvette, France"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Servant", 
            "givenName": "Pascale", 
            "id": "sg:person.01003705465.41", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01003705465.41"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "French National Centre for Scientific Research", 
              "id": "https://www.grid.ac/institutes/grid.4444.0", 
              "name": [
                "Institut Interdisciplinaire de Neurosciences, University of Bordeaux, Bordeaux, France", 
                "Centre National de la Recherche Scientifique, UMR5297, Bordeaux, France"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Galland", 
            "givenName": "Remi", 
            "id": "sg:person.01247032361.39", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01247032361.39"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "French National Centre for Scientific Research", 
              "id": "https://www.grid.ac/institutes/grid.4444.0", 
              "name": [
                "Institut Interdisciplinaire de Neurosciences, University of Bordeaux, Bordeaux, France", 
                "Centre National de la Recherche Scientifique, UMR5297, Bordeaux, France"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Butler", 
            "givenName": "Corey", 
            "id": "sg:person.01161736325.08", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01161736325.08"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "French National Centre for Scientific Research", 
              "id": "https://www.grid.ac/institutes/grid.4444.0", 
              "name": [
                "Institut Interdisciplinaire de Neurosciences, University of Bordeaux, Bordeaux, France", 
                "Centre National de la Recherche Scientifique, UMR5297, Bordeaux, France"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Sibarita", 
            "givenName": "Jean-Baptiste", 
            "id": "sg:person.01074756450.42", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01074756450.42"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Grenoble Alpes University", 
              "id": "https://www.grid.ac/institutes/grid.450307.5", 
              "name": [
                "Univ. Grenoble Alpes, CEA, CNRS, IBS, F-38000, Grenoble, France"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Bourgeois", 
            "givenName": "Dominique", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Grenoble Alpes University", 
              "id": "https://www.grid.ac/institutes/grid.450307.5", 
              "name": [
                "Univ. Grenoble Alpes, CEA, CNRS, IBS, F-38000, Grenoble, France"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Timmins", 
            "givenName": "Joanna", 
            "id": "sg:person.01342450564.70", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01342450564.70"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1073/pnas.1507386112", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001476982"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1153529", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005581664"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1088/2050-6120/3/1/014005", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006672303"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.2448", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006928937", 
              "https://doi.org/10.1038/nmeth.2448"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/cphc.201600035", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009284165"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.3579", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011454519", 
              "https://doi.org/10.1038/nmeth.3579"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bpj.2016.01.010", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011504110"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bpj.2013.05.048", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011788072"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.3390/molecules190812116", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012032563"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0098362", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012160586"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth929", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018882864", 
              "https://doi.org/10.1038/nmeth929"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth929", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018882864", 
              "https://doi.org/10.1038/nmeth929"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/mmbr.65.1.44-79.2001", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021785027"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1127344", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022108219"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/cmi.12024", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023524709"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10529-010-0422-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024215284", 
              "https://doi.org/10.1007/s10529-010-0422-6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1103/physreve.86.021907", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024747410"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1103/physreve.86.021907", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024747410"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/mmbr.00015-10", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026612882"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.2019", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026706357", 
              "https://doi.org/10.1038/nmeth.2019"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00408143", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027398559", 
              "https://doi.org/10.1007/bf00408143"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00408143", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027398559", 
              "https://doi.org/10.1007/bf00408143"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jsb.2013.05.004", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028756140"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1756-0500-7-274", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029240027", 
              "https://doi.org/10.1186/1756-0500-7-274"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bbamem.2014.02.014", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030701887"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1529/biophysj.106.091116", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032286860"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1080/10409230802122274", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032775763"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.tim.2010.07.007", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032940067"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/bioinformatics/btu202", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033447186"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1077865", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034010488"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0009-2614(89)87342-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036033171"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0609643104", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036737982"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/aem.66.6.2620-2626.2000", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038551419"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.1176", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038882342", 
              "https://doi.org/10.1038/nmeth.1176"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.3528", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038928421", 
              "https://doi.org/10.1038/nmeth.3528"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrmicro3154", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040386061", 
              "https://doi.org/10.1038/nrmicro3154"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.3389/fmicb.2016.00155", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040887187"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0012680", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042319234"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ncomms12568", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042518294", 
              "https://doi.org/10.1038/ncomms12568"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00203-007-0262-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042553310", 
              "https://doi.org/10.1007/s00203-007-0262-5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pgen.1000815", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043406841"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0604503103", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048906758"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.micro.51.1.203", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052635101"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1139/m83-217", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053010462"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.csbj.2016.04.001", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053399333"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.1507592112", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1058212222"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1099/ijs.0.63717-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060386779"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1099/mic.0.000186", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060394090"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1099/mic.0.28971-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060397792"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1081238253", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bpj.2017.02.028", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1084754472"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/anie.201706121", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1090285734"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/anie.201706121", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1090285734"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/acs.jpclett.7b01701", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1091433752"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/s41467-017-00847-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1092170287", 
              "https://doi.org/10.1038/s41467-017-00847-3"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2018-12", 
        "datePublishedReg": "2018-12-01", 
        "description": "Spurious blinking fluorescent spots are often seen in bacteria during single-molecule localization microscopy experiments. Although this 'autoblinking' phenomenon is widespread, its origin remains unclear. In Deinococcus strains, we observed particularly strong autoblinking at the periphery of the bacteria, facilitating its comprehensive characterization. A systematic evaluation of the contributions of different components of the sample environment to autoblinking levels and the in-depth analysis of the photophysical properties of autoblinking molecules indicate that the phenomenon results from transient binding of fluorophores originating mostly from the growth medium to the bacterial cell wall, which produces single-molecule fluorescence through a Point Accumulation for Imaging in Nanoscale Topography (PAINT) mechanism. Our data suggest that the autoblinking molecules preferentially bind to the plasma membrane of bacterial cells. Autoblinking microscopy was used to acquire nanoscale images of live, unlabeled D. radiodurans and could be combined with PALM imaging of PAmCherry-labeled bacteria in two-color experiments. Autoblinking-based super-resolved images provided insight into the formation of septa in dividing bacteria and revealed heterogeneities in the distribution and dynamics of autoblinking molecules within the cell wall.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/s41598-018-32335-z", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isPartOf": [
          {
            "id": "sg:journal.1045337", 
            "issn": [
              "2045-2322"
            ], 
            "name": "Scientific Reports", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "8"
          }
        ], 
        "name": "Bacterial cell wall nanoimaging by autoblinking microscopy", 
        "pagination": "14038", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "11b141d8ff4ab0b0bdd68577c36effa11c92bf6361bb2b111f5045b1f9dd415f"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "30232348"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "101563288"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/s41598-018-32335-z"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1106971227"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/s41598-018-32335-z", 
          "https://app.dimensions.ai/details/publication/pub.1106971227"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T16:05", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8664_00000609.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://www.nature.com/articles/s41598-018-32335-z"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-32335-z'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-32335-z'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-32335-z'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-32335-z'


     

    This table displays all metadata directly associated to this object as RDF triples.

    293 TRIPLES      21 PREDICATES      80 URIs      21 LITERALS      9 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/s41598-018-32335-z schema:about anzsrc-for:06
    2 anzsrc-for:0601
    3 schema:author Nf93e15a62f6b4e94a7141edb047851df
    4 schema:citation sg:pub.10.1007/bf00408143
    5 sg:pub.10.1007/s00203-007-0262-5
    6 sg:pub.10.1007/s10529-010-0422-6
    7 sg:pub.10.1038/ncomms12568
    8 sg:pub.10.1038/nmeth.1176
    9 sg:pub.10.1038/nmeth.2019
    10 sg:pub.10.1038/nmeth.2448
    11 sg:pub.10.1038/nmeth.3528
    12 sg:pub.10.1038/nmeth.3579
    13 sg:pub.10.1038/nmeth929
    14 sg:pub.10.1038/nrmicro3154
    15 sg:pub.10.1038/s41467-017-00847-3
    16 sg:pub.10.1186/1756-0500-7-274
    17 https://app.dimensions.ai/details/publication/pub.1081238253
    18 https://doi.org/10.1002/anie.201706121
    19 https://doi.org/10.1002/cphc.201600035
    20 https://doi.org/10.1016/0009-2614(89)87342-7
    21 https://doi.org/10.1016/j.bbamem.2014.02.014
    22 https://doi.org/10.1016/j.bpj.2013.05.048
    23 https://doi.org/10.1016/j.bpj.2016.01.010
    24 https://doi.org/10.1016/j.bpj.2017.02.028
    25 https://doi.org/10.1016/j.csbj.2016.04.001
    26 https://doi.org/10.1016/j.jsb.2013.05.004
    27 https://doi.org/10.1016/j.tim.2010.07.007
    28 https://doi.org/10.1021/acs.jpclett.7b01701
    29 https://doi.org/10.1073/pnas.0604503103
    30 https://doi.org/10.1073/pnas.0609643104
    31 https://doi.org/10.1073/pnas.1507386112
    32 https://doi.org/10.1073/pnas.1507592112
    33 https://doi.org/10.1080/10409230802122274
    34 https://doi.org/10.1088/2050-6120/3/1/014005
    35 https://doi.org/10.1093/bioinformatics/btu202
    36 https://doi.org/10.1099/ijs.0.63717-0
    37 https://doi.org/10.1099/mic.0.000186
    38 https://doi.org/10.1099/mic.0.28971-0
    39 https://doi.org/10.1103/physreve.86.021907
    40 https://doi.org/10.1111/cmi.12024
    41 https://doi.org/10.1126/science.1077865
    42 https://doi.org/10.1126/science.1127344
    43 https://doi.org/10.1126/science.1153529
    44 https://doi.org/10.1128/aem.66.6.2620-2626.2000
    45 https://doi.org/10.1128/mmbr.00015-10
    46 https://doi.org/10.1128/mmbr.65.1.44-79.2001
    47 https://doi.org/10.1139/m83-217
    48 https://doi.org/10.1146/annurev.micro.51.1.203
    49 https://doi.org/10.1371/journal.pgen.1000815
    50 https://doi.org/10.1371/journal.pone.0012680
    51 https://doi.org/10.1371/journal.pone.0098362
    52 https://doi.org/10.1529/biophysj.106.091116
    53 https://doi.org/10.3389/fmicb.2016.00155
    54 https://doi.org/10.3390/molecules190812116
    55 schema:datePublished 2018-12
    56 schema:datePublishedReg 2018-12-01
    57 schema:description Spurious blinking fluorescent spots are often seen in bacteria during single-molecule localization microscopy experiments. Although this 'autoblinking' phenomenon is widespread, its origin remains unclear. In Deinococcus strains, we observed particularly strong autoblinking at the periphery of the bacteria, facilitating its comprehensive characterization. A systematic evaluation of the contributions of different components of the sample environment to autoblinking levels and the in-depth analysis of the photophysical properties of autoblinking molecules indicate that the phenomenon results from transient binding of fluorophores originating mostly from the growth medium to the bacterial cell wall, which produces single-molecule fluorescence through a Point Accumulation for Imaging in Nanoscale Topography (PAINT) mechanism. Our data suggest that the autoblinking molecules preferentially bind to the plasma membrane of bacterial cells. Autoblinking microscopy was used to acquire nanoscale images of live, unlabeled D. radiodurans and could be combined with PALM imaging of PAmCherry-labeled bacteria in two-color experiments. Autoblinking-based super-resolved images provided insight into the formation of septa in dividing bacteria and revealed heterogeneities in the distribution and dynamics of autoblinking molecules within the cell wall.
    58 schema:genre research_article
    59 schema:inLanguage en
    60 schema:isAccessibleForFree true
    61 schema:isPartOf N2c792c9cc9ff4cd0bcc028326a612711
    62 N2f6c28a841cc47f7a21e9562485eb1ad
    63 sg:journal.1045337
    64 schema:name Bacterial cell wall nanoimaging by autoblinking microscopy
    65 schema:pagination 14038
    66 schema:productId N20de6a1c2179409aae23583a133fba82
    67 N57df783d603e4e43a6f8c04a42f6019b
    68 N939a6aacb0bf49a0ab0af944a0a90cad
    69 Ncab2926d55ce4131bdc2777066e15d42
    70 Nfff171888ec546a3a68456087351ad47
    71 schema:sameAs https://app.dimensions.ai/details/publication/pub.1106971227
    72 https://doi.org/10.1038/s41598-018-32335-z
    73 schema:sdDatePublished 2019-04-10T16:05
    74 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    75 schema:sdPublisher Necc71fafecf6475da5325d2b08bd91f0
    76 schema:url https://www.nature.com/articles/s41598-018-32335-z
    77 sgo:license sg:explorer/license/
    78 sgo:sdDataset articles
    79 rdf:type schema:ScholarlyArticle
    80 N04720bb1a93a4b39a2872d471a8f415b rdf:first sg:person.01342450564.70
    81 rdf:rest rdf:nil
    82 N08ea53b3688247fc8b7d39b67b6e4cbd rdf:first N15e9bbccae0c44d2b0e41293862f1380
    83 rdf:rest N5118e2e617f2489ebb20ec56305e66f5
    84 N0e18fd78c183491c801912c0188a0caa schema:affiliation https://www.grid.ac/institutes/grid.450307.5
    85 schema:familyName Bourgeois
    86 schema:givenName Dominique
    87 rdf:type schema:Person
    88 N15e9bbccae0c44d2b0e41293862f1380 schema:affiliation https://www.grid.ac/institutes/grid.450307.5
    89 schema:familyName Lacroix
    90 schema:givenName Françoise
    91 rdf:type schema:Person
    92 N1d04b97f23b94f16aef4796aa79ee596 schema:affiliation https://www.grid.ac/institutes/grid.450307.5
    93 schema:familyName Barbieri
    94 schema:givenName Liliana
    95 rdf:type schema:Person
    96 N20de6a1c2179409aae23583a133fba82 schema:name dimensions_id
    97 schema:value pub.1106971227
    98 rdf:type schema:PropertyValue
    99 N2c792c9cc9ff4cd0bcc028326a612711 schema:issueNumber 1
    100 rdf:type schema:PublicationIssue
    101 N2f6c28a841cc47f7a21e9562485eb1ad schema:volumeNumber 8
    102 rdf:type schema:PublicationVolume
    103 N4e852944b670440c96e67a355d811ddf rdf:first sg:person.01161736325.08
    104 rdf:rest Nd64d594e59c548dfa46c57ea5209f38b
    105 N5118e2e617f2489ebb20ec56305e66f5 rdf:first N1d04b97f23b94f16aef4796aa79ee596
    106 rdf:rest Na1c24d9bc8d74ff784e9755e45bf3445
    107 N57df783d603e4e43a6f8c04a42f6019b schema:name readcube_id
    108 schema:value 11b141d8ff4ab0b0bdd68577c36effa11c92bf6361bb2b111f5045b1f9dd415f
    109 rdf:type schema:PropertyValue
    110 N907de19bc44f4361811ce6ff0e59cc5a rdf:first sg:person.01247032361.39
    111 rdf:rest N4e852944b670440c96e67a355d811ddf
    112 N939a6aacb0bf49a0ab0af944a0a90cad schema:name pubmed_id
    113 schema:value 30232348
    114 rdf:type schema:PropertyValue
    115 Na1c24d9bc8d74ff784e9755e45bf3445 rdf:first sg:person.01003705465.41
    116 rdf:rest N907de19bc44f4361811ce6ff0e59cc5a
    117 Ncab2926d55ce4131bdc2777066e15d42 schema:name doi
    118 schema:value 10.1038/s41598-018-32335-z
    119 rdf:type schema:PropertyValue
    120 Nd64d594e59c548dfa46c57ea5209f38b rdf:first sg:person.01074756450.42
    121 rdf:rest Ne92588bd8db14b6691833b78355e05b7
    122 Ne92588bd8db14b6691833b78355e05b7 rdf:first N0e18fd78c183491c801912c0188a0caa
    123 rdf:rest N04720bb1a93a4b39a2872d471a8f415b
    124 Necc71fafecf6475da5325d2b08bd91f0 schema:name Springer Nature - SN SciGraph project
    125 rdf:type schema:Organization
    126 Nf93e15a62f6b4e94a7141edb047851df rdf:first sg:person.012567422720.30
    127 rdf:rest N08ea53b3688247fc8b7d39b67b6e4cbd
    128 Nfff171888ec546a3a68456087351ad47 schema:name nlm_unique_id
    129 schema:value 101563288
    130 rdf:type schema:PropertyValue
    131 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    132 schema:name Biological Sciences
    133 rdf:type schema:DefinedTerm
    134 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    135 schema:name Biochemistry and Cell Biology
    136 rdf:type schema:DefinedTerm
    137 sg:journal.1045337 schema:issn 2045-2322
    138 schema:name Scientific Reports
    139 rdf:type schema:Periodical
    140 sg:person.01003705465.41 schema:affiliation https://www.grid.ac/institutes/grid.462411.4
    141 schema:familyName Servant
    142 schema:givenName Pascale
    143 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01003705465.41
    144 rdf:type schema:Person
    145 sg:person.01074756450.42 schema:affiliation https://www.grid.ac/institutes/grid.4444.0
    146 schema:familyName Sibarita
    147 schema:givenName Jean-Baptiste
    148 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01074756450.42
    149 rdf:type schema:Person
    150 sg:person.01161736325.08 schema:affiliation https://www.grid.ac/institutes/grid.4444.0
    151 schema:familyName Butler
    152 schema:givenName Corey
    153 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01161736325.08
    154 rdf:type schema:Person
    155 sg:person.01247032361.39 schema:affiliation https://www.grid.ac/institutes/grid.4444.0
    156 schema:familyName Galland
    157 schema:givenName Remi
    158 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01247032361.39
    159 rdf:type schema:Person
    160 sg:person.012567422720.30 schema:affiliation https://www.grid.ac/institutes/grid.450307.5
    161 schema:familyName Floc’h
    162 schema:givenName Kevin
    163 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012567422720.30
    164 rdf:type schema:Person
    165 sg:person.01342450564.70 schema:affiliation https://www.grid.ac/institutes/grid.450307.5
    166 schema:familyName Timmins
    167 schema:givenName Joanna
    168 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01342450564.70
    169 rdf:type schema:Person
    170 sg:pub.10.1007/bf00408143 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027398559
    171 https://doi.org/10.1007/bf00408143
    172 rdf:type schema:CreativeWork
    173 sg:pub.10.1007/s00203-007-0262-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042553310
    174 https://doi.org/10.1007/s00203-007-0262-5
    175 rdf:type schema:CreativeWork
    176 sg:pub.10.1007/s10529-010-0422-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024215284
    177 https://doi.org/10.1007/s10529-010-0422-6
    178 rdf:type schema:CreativeWork
    179 sg:pub.10.1038/ncomms12568 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042518294
    180 https://doi.org/10.1038/ncomms12568
    181 rdf:type schema:CreativeWork
    182 sg:pub.10.1038/nmeth.1176 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038882342
    183 https://doi.org/10.1038/nmeth.1176
    184 rdf:type schema:CreativeWork
    185 sg:pub.10.1038/nmeth.2019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026706357
    186 https://doi.org/10.1038/nmeth.2019
    187 rdf:type schema:CreativeWork
    188 sg:pub.10.1038/nmeth.2448 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006928937
    189 https://doi.org/10.1038/nmeth.2448
    190 rdf:type schema:CreativeWork
    191 sg:pub.10.1038/nmeth.3528 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038928421
    192 https://doi.org/10.1038/nmeth.3528
    193 rdf:type schema:CreativeWork
    194 sg:pub.10.1038/nmeth.3579 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011454519
    195 https://doi.org/10.1038/nmeth.3579
    196 rdf:type schema:CreativeWork
    197 sg:pub.10.1038/nmeth929 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018882864
    198 https://doi.org/10.1038/nmeth929
    199 rdf:type schema:CreativeWork
    200 sg:pub.10.1038/nrmicro3154 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040386061
    201 https://doi.org/10.1038/nrmicro3154
    202 rdf:type schema:CreativeWork
    203 sg:pub.10.1038/s41467-017-00847-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1092170287
    204 https://doi.org/10.1038/s41467-017-00847-3
    205 rdf:type schema:CreativeWork
    206 sg:pub.10.1186/1756-0500-7-274 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029240027
    207 https://doi.org/10.1186/1756-0500-7-274
    208 rdf:type schema:CreativeWork
    209 https://app.dimensions.ai/details/publication/pub.1081238253 schema:CreativeWork
    210 https://doi.org/10.1002/anie.201706121 schema:sameAs https://app.dimensions.ai/details/publication/pub.1090285734
    211 rdf:type schema:CreativeWork
    212 https://doi.org/10.1002/cphc.201600035 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009284165
    213 rdf:type schema:CreativeWork
    214 https://doi.org/10.1016/0009-2614(89)87342-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036033171
    215 rdf:type schema:CreativeWork
    216 https://doi.org/10.1016/j.bbamem.2014.02.014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030701887
    217 rdf:type schema:CreativeWork
    218 https://doi.org/10.1016/j.bpj.2013.05.048 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011788072
    219 rdf:type schema:CreativeWork
    220 https://doi.org/10.1016/j.bpj.2016.01.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011504110
    221 rdf:type schema:CreativeWork
    222 https://doi.org/10.1016/j.bpj.2017.02.028 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084754472
    223 rdf:type schema:CreativeWork
    224 https://doi.org/10.1016/j.csbj.2016.04.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053399333
    225 rdf:type schema:CreativeWork
    226 https://doi.org/10.1016/j.jsb.2013.05.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028756140
    227 rdf:type schema:CreativeWork
    228 https://doi.org/10.1016/j.tim.2010.07.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032940067
    229 rdf:type schema:CreativeWork
    230 https://doi.org/10.1021/acs.jpclett.7b01701 schema:sameAs https://app.dimensions.ai/details/publication/pub.1091433752
    231 rdf:type schema:CreativeWork
    232 https://doi.org/10.1073/pnas.0604503103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048906758
    233 rdf:type schema:CreativeWork
    234 https://doi.org/10.1073/pnas.0609643104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036737982
    235 rdf:type schema:CreativeWork
    236 https://doi.org/10.1073/pnas.1507386112 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001476982
    237 rdf:type schema:CreativeWork
    238 https://doi.org/10.1073/pnas.1507592112 schema:sameAs https://app.dimensions.ai/details/publication/pub.1058212222
    239 rdf:type schema:CreativeWork
    240 https://doi.org/10.1080/10409230802122274 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032775763
    241 rdf:type schema:CreativeWork
    242 https://doi.org/10.1088/2050-6120/3/1/014005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006672303
    243 rdf:type schema:CreativeWork
    244 https://doi.org/10.1093/bioinformatics/btu202 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033447186
    245 rdf:type schema:CreativeWork
    246 https://doi.org/10.1099/ijs.0.63717-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060386779
    247 rdf:type schema:CreativeWork
    248 https://doi.org/10.1099/mic.0.000186 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060394090
    249 rdf:type schema:CreativeWork
    250 https://doi.org/10.1099/mic.0.28971-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060397792
    251 rdf:type schema:CreativeWork
    252 https://doi.org/10.1103/physreve.86.021907 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024747410
    253 rdf:type schema:CreativeWork
    254 https://doi.org/10.1111/cmi.12024 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023524709
    255 rdf:type schema:CreativeWork
    256 https://doi.org/10.1126/science.1077865 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034010488
    257 rdf:type schema:CreativeWork
    258 https://doi.org/10.1126/science.1127344 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022108219
    259 rdf:type schema:CreativeWork
    260 https://doi.org/10.1126/science.1153529 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005581664
    261 rdf:type schema:CreativeWork
    262 https://doi.org/10.1128/aem.66.6.2620-2626.2000 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038551419
    263 rdf:type schema:CreativeWork
    264 https://doi.org/10.1128/mmbr.00015-10 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026612882
    265 rdf:type schema:CreativeWork
    266 https://doi.org/10.1128/mmbr.65.1.44-79.2001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021785027
    267 rdf:type schema:CreativeWork
    268 https://doi.org/10.1139/m83-217 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053010462
    269 rdf:type schema:CreativeWork
    270 https://doi.org/10.1146/annurev.micro.51.1.203 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052635101
    271 rdf:type schema:CreativeWork
    272 https://doi.org/10.1371/journal.pgen.1000815 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043406841
    273 rdf:type schema:CreativeWork
    274 https://doi.org/10.1371/journal.pone.0012680 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042319234
    275 rdf:type schema:CreativeWork
    276 https://doi.org/10.1371/journal.pone.0098362 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012160586
    277 rdf:type schema:CreativeWork
    278 https://doi.org/10.1529/biophysj.106.091116 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032286860
    279 rdf:type schema:CreativeWork
    280 https://doi.org/10.3389/fmicb.2016.00155 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040887187
    281 rdf:type schema:CreativeWork
    282 https://doi.org/10.3390/molecules190812116 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012032563
    283 rdf:type schema:CreativeWork
    284 https://www.grid.ac/institutes/grid.4444.0 schema:alternateName French National Centre for Scientific Research
    285 schema:name Centre National de la Recherche Scientifique, UMR5297, Bordeaux, France
    286 Institut Interdisciplinaire de Neurosciences, University of Bordeaux, Bordeaux, France
    287 rdf:type schema:Organization
    288 https://www.grid.ac/institutes/grid.450307.5 schema:alternateName Grenoble Alpes University
    289 schema:name Univ. Grenoble Alpes, CEA, CNRS, IBS, F-38000, Grenoble, France
    290 rdf:type schema:Organization
    291 https://www.grid.ac/institutes/grid.462411.4 schema:alternateName Institute of Integrative Biology of the Cell
    292 schema:name Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay, 91198 Gif-sur-Yvette, France
    293 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...