Co-culture models illustrate the digestion of Gemmata spp. by phagocytes View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-12

AUTHORS

Odilon D. Kaboré, Ahmed Loukil, Sylvain Godreuil, Michel Drancourt

ABSTRACT

Gemmata spp. bacteria thrive in the same aquatic environments as free-living amoebae. DNA-based detection of Gemmata spp. sequences in the microbiota of the human digestive tract and blood further questioned the susceptibility of Gemmata spp. to phagocytes. Here, Gemmata obscuriglobus and Gemmata massiliana were co-cultured with the amoebae Acanthamoeba polyphaga, Acanthamoeba castellanii, Acanthamoeba griffini and THP-1 macrophage-like phagocytes. All experiments were performed in five independant replicates. The ratio amoeba/bacteria was 1:20 and the ratio THP-1/bacteria was 1:10. After a 2-hour co-culture, extracellular bacteria were killed by kanamycin or amikacin and eliminated. The intracellular location of Gemmata bacteria was specified by confocal microscopy. Microscopic enumerations and culture-based enumerations of colony-forming units were performed at T = 0, 1, 2, 3, 4, 8, 16, 24, 48 and 72 hours post-infection. Then, Gemmata bacteria were engulfed into the phagocytes' cytoplasmic vacuoles, more than (98 ± 2)% of Gemmata bacteria, compared to controls, were destroyed by phagocytic cells after a 48-h co-culture according to microscopy and culture results, and no positive culture was observed at T = 72-hours. Under our co-culture conditions, Gemmata bacteria were therefore susceptible to the environmental and host phagocytes here investigated. These data suggest that these Acanthamoeba species and THP-1 cells cannot be used to isolate G. massiliana and G. obscuriglobus under the co-culture conditions applied in this study. Although the THP-1 response can point towards potential responses that might occur in vivo, these responses should first bevalidated by in vivo studies to draw definite conclusions. More... »

PAGES

13311

References to SciGraph publications

Journal

TITLE

Scientific Reports

ISSUE

1

VOLUME

8

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-018-31667-0

DOI

http://dx.doi.org/10.1038/s41598-018-31667-0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1106476140

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30190504


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0605", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "name": [
            "Aix Marseille Univ., IRD, MEPHI, IHU M\u00e9diterran\u00e9e Infection, Marseille, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kabor\u00e9", 
        "givenName": "Odilon D.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Aix Marseille Univ., IRD, MEPHI, IHU M\u00e9diterran\u00e9e Infection, Marseille, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Loukil", 
        "givenName": "Ahmed", 
        "id": "sg:person.011045203524.70", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011045203524.70"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Montpellier", 
          "id": "https://www.grid.ac/institutes/grid.121334.6", 
          "name": [
            "Universit\u00e9 de Montpellier UMR 1058 UMR MIVEGEC, UMR IRD 224-CNRS Inserm, 1058, Montpellier, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Godreuil", 
        "givenName": "Sylvain", 
        "id": "sg:person.01033506033.12", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01033506033.12"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Aix Marseille Univ., IRD, MEPHI, IHU M\u00e9diterran\u00e9e Infection, Marseille, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Drancourt", 
        "givenName": "Michel", 
        "id": "sg:person.01106456741.46", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01106456741.46"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1371/journal.pone.0029833", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001443274"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrmicro2578", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001868665", 
          "https://doi.org/10.1038/nrmicro2578"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1758-2229.2010.00176.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002299152"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1758-2229.2010.00176.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002299152"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ijantimicag.2012.10.012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004055102"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.copbio.2006.05.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004611689"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.03765-12", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004975063"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s40793-015-0103-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006573317", 
          "https://doi.org/10.1186/s40793-015-0103-0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.micinf.2016.12.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007415728"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.72.1.854-859.2006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010760000"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00436-016-5271-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012057373", 
          "https://doi.org/10.1007/s00436-016-5271-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00436-016-5271-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012057373", 
          "https://doi.org/10.1007/s00436-016-5271-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1574-6941.2011.01168.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013545521"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jcm.01207-14", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015287109"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1469-0691.2001.00193.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017593178"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1749-6632.2003.tb07437.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020652547"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1749-6632.2003.tb07437.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020652547"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0723-2020(11)80042-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022410864"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10482-013-0003-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023333963", 
          "https://doi.org/10.1007/s10482-013-0003-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10482-013-0003-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023333963", 
          "https://doi.org/10.1007/s10482-013-0003-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.69.7.4316-4319.2003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023688584"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.01332-08", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028485810"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3389/fmicb.2012.00405", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029054147"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.00699-11", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030274682"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/1574-6941.12258", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033003140"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.71.1.20-28.2005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033455941"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jcm.42.12.5493-5501.2004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033831086"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s13062-015-0041-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034470611", 
          "https://doi.org/10.1186/s13062-015-0041-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s13062-015-0041-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034470611", 
          "https://doi.org/10.1186/s13062-015-0041-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/apm.12087", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034657304"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.68.1.417-422.2002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035349305"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/cmr.17.2.413-433.2004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036628596"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1136/jcp.33.12.1179", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039335442"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2217/fmb-2015-0001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040167149"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02342136", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041742554", 
          "https://doi.org/10.1007/bf02342136"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02342136", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041742554", 
          "https://doi.org/10.1007/bf02342136"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/jac/dkq290", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042238044"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/jac/dkq290", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042238044"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.70.9.5094-5101.2004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044239623"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1086/432487", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046307325"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/ijc.2910260208", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048280380"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00436-014-3976-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048766059", 
          "https://doi.org/10.1007/s00436-014-3976-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00436-014-3976-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048766059", 
          "https://doi.org/10.1007/s00436-014-3976-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1469-0691.2009.03011.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051094989"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/es0604257", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055499169"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/es0604257", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055499169"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/13500872-141-7-1493", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060380874"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3354/ame033239", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1071157860"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082060301", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083024132", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.00593-17", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085292782"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2018-12", 
    "datePublishedReg": "2018-12-01", 
    "description": "Gemmata spp. bacteria thrive in the same aquatic environments as free-living amoebae. DNA-based detection of Gemmata spp. sequences in the microbiota of the human digestive tract and blood further questioned the susceptibility of Gemmata spp. to phagocytes. Here, Gemmata obscuriglobus and Gemmata massiliana were co-cultured with the amoebae Acanthamoeba polyphaga, Acanthamoeba castellanii, Acanthamoeba griffini and THP-1 macrophage-like phagocytes. All experiments were performed in five independant replicates. The ratio amoeba/bacteria was 1:20 and the ratio THP-1/bacteria was 1:10. After a 2-hour co-culture, extracellular bacteria were killed by kanamycin or amikacin and eliminated. The intracellular location of Gemmata bacteria was specified by confocal microscopy. Microscopic enumerations and culture-based enumerations of colony-forming units were performed at T\u2009=\u20090, 1, 2, 3, 4, 8, 16, 24, 48 and 72\u2009hours post-infection. Then, Gemmata bacteria were engulfed into the phagocytes' cytoplasmic vacuoles, more than (98\u2009\u00b1\u20092)% of Gemmata bacteria, compared to controls, were destroyed by phagocytic cells after a 48-h co-culture according to microscopy and culture results, and no positive culture was observed at T\u2009=\u200972-hours. Under our co-culture conditions, Gemmata bacteria were therefore susceptible to the environmental and host phagocytes here investigated. These data suggest that these Acanthamoeba species and THP-1 cells cannot be used to isolate G. massiliana and G. obscuriglobus under the co-culture conditions applied in this study. Although the THP-1 response can point towards potential responses that might occur in vivo, these responses should first bevalidated by in vivo studies to draw definite conclusions.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/s41598-018-31667-0", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1045337", 
        "issn": [
          "2045-2322"
        ], 
        "name": "Scientific Reports", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "8"
      }
    ], 
    "name": "Co-culture models illustrate the digestion of Gemmata spp. by phagocytes", 
    "pagination": "13311", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "0a0c9e925e2efd4228a96eb1c1b32af3a191a97c30aa9ee66ab52e7413b4ba63"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30190504"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101563288"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/s41598-018-31667-0"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1106476140"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/s41598-018-31667-0", 
      "https://app.dimensions.ai/details/publication/pub.1106476140"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T17:40", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8672_00000571.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/s41598-018-31667-0"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-31667-0'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-31667-0'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-31667-0'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-31667-0'


 

This table displays all metadata directly associated to this object as RDF triples.

225 TRIPLES      21 PREDICATES      71 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/s41598-018-31667-0 schema:about anzsrc-for:06
2 anzsrc-for:0605
3 schema:author Neb9ebd391bba49fbb1babd95dadc7b68
4 schema:citation sg:pub.10.1007/bf02342136
5 sg:pub.10.1007/s00436-014-3976-8
6 sg:pub.10.1007/s00436-016-5271-3
7 sg:pub.10.1007/s10482-013-0003-5
8 sg:pub.10.1038/nrmicro2578
9 sg:pub.10.1186/s13062-015-0041-1
10 sg:pub.10.1186/s40793-015-0103-0
11 https://app.dimensions.ai/details/publication/pub.1082060301
12 https://app.dimensions.ai/details/publication/pub.1083024132
13 https://doi.org/10.1002/ijc.2910260208
14 https://doi.org/10.1016/j.copbio.2006.05.005
15 https://doi.org/10.1016/j.ijantimicag.2012.10.012
16 https://doi.org/10.1016/j.micinf.2016.12.005
17 https://doi.org/10.1016/s0723-2020(11)80042-1
18 https://doi.org/10.1021/es0604257
19 https://doi.org/10.1046/j.1469-0691.2001.00193.x
20 https://doi.org/10.1086/432487
21 https://doi.org/10.1093/jac/dkq290
22 https://doi.org/10.1099/13500872-141-7-1493
23 https://doi.org/10.1111/1574-6941.12258
24 https://doi.org/10.1111/apm.12087
25 https://doi.org/10.1111/j.1469-0691.2009.03011.x
26 https://doi.org/10.1111/j.1574-6941.2011.01168.x
27 https://doi.org/10.1111/j.1749-6632.2003.tb07437.x
28 https://doi.org/10.1111/j.1758-2229.2010.00176.x
29 https://doi.org/10.1128/aem.00593-17
30 https://doi.org/10.1128/aem.00699-11
31 https://doi.org/10.1128/aem.01332-08
32 https://doi.org/10.1128/aem.03765-12
33 https://doi.org/10.1128/aem.68.1.417-422.2002
34 https://doi.org/10.1128/aem.69.7.4316-4319.2003
35 https://doi.org/10.1128/aem.70.9.5094-5101.2004
36 https://doi.org/10.1128/aem.71.1.20-28.2005
37 https://doi.org/10.1128/aem.72.1.854-859.2006
38 https://doi.org/10.1128/cmr.17.2.413-433.2004
39 https://doi.org/10.1128/jcm.01207-14
40 https://doi.org/10.1128/jcm.42.12.5493-5501.2004
41 https://doi.org/10.1136/jcp.33.12.1179
42 https://doi.org/10.1371/journal.pone.0029833
43 https://doi.org/10.2217/fmb-2015-0001
44 https://doi.org/10.3354/ame033239
45 https://doi.org/10.3389/fmicb.2012.00405
46 schema:datePublished 2018-12
47 schema:datePublishedReg 2018-12-01
48 schema:description Gemmata spp. bacteria thrive in the same aquatic environments as free-living amoebae. DNA-based detection of Gemmata spp. sequences in the microbiota of the human digestive tract and blood further questioned the susceptibility of Gemmata spp. to phagocytes. Here, Gemmata obscuriglobus and Gemmata massiliana were co-cultured with the amoebae Acanthamoeba polyphaga, Acanthamoeba castellanii, Acanthamoeba griffini and THP-1 macrophage-like phagocytes. All experiments were performed in five independant replicates. The ratio amoeba/bacteria was 1:20 and the ratio THP-1/bacteria was 1:10. After a 2-hour co-culture, extracellular bacteria were killed by kanamycin or amikacin and eliminated. The intracellular location of Gemmata bacteria was specified by confocal microscopy. Microscopic enumerations and culture-based enumerations of colony-forming units were performed at T = 0, 1, 2, 3, 4, 8, 16, 24, 48 and 72 hours post-infection. Then, Gemmata bacteria were engulfed into the phagocytes' cytoplasmic vacuoles, more than (98 ± 2)% of Gemmata bacteria, compared to controls, were destroyed by phagocytic cells after a 48-h co-culture according to microscopy and culture results, and no positive culture was observed at T = 72-hours. Under our co-culture conditions, Gemmata bacteria were therefore susceptible to the environmental and host phagocytes here investigated. These data suggest that these Acanthamoeba species and THP-1 cells cannot be used to isolate G. massiliana and G. obscuriglobus under the co-culture conditions applied in this study. Although the THP-1 response can point towards potential responses that might occur in vivo, these responses should first bevalidated by in vivo studies to draw definite conclusions.
49 schema:genre research_article
50 schema:inLanguage en
51 schema:isAccessibleForFree true
52 schema:isPartOf Naea5744b657a4f758a20dc10f118ed5d
53 Nbf10722e10fb405297368b2e89963203
54 sg:journal.1045337
55 schema:name Co-culture models illustrate the digestion of Gemmata spp. by phagocytes
56 schema:pagination 13311
57 schema:productId N30b8d8a4f5614ffea4465c15a810b705
58 N3ff1d19d41b940e29f958c1c9e5d268c
59 N5cc33212ee6247e29556e1e5a00359ab
60 Naf7a04c9a39045afb166cad636f49279
61 Ne6d7b9326f31478192ecff17a5e5b39a
62 schema:sameAs https://app.dimensions.ai/details/publication/pub.1106476140
63 https://doi.org/10.1038/s41598-018-31667-0
64 schema:sdDatePublished 2019-04-10T17:40
65 schema:sdLicense https://scigraph.springernature.com/explorer/license/
66 schema:sdPublisher N76691f989aca4c40898045bc30575967
67 schema:url https://www.nature.com/articles/s41598-018-31667-0
68 sgo:license sg:explorer/license/
69 sgo:sdDataset articles
70 rdf:type schema:ScholarlyArticle
71 N0f5fc546a83a42a1b478cb273f5a2224 rdf:first sg:person.011045203524.70
72 rdf:rest N6bcb2e9f8e5a4cada4904796e3bb0871
73 N30b8d8a4f5614ffea4465c15a810b705 schema:name readcube_id
74 schema:value 0a0c9e925e2efd4228a96eb1c1b32af3a191a97c30aa9ee66ab52e7413b4ba63
75 rdf:type schema:PropertyValue
76 N3ff1d19d41b940e29f958c1c9e5d268c schema:name nlm_unique_id
77 schema:value 101563288
78 rdf:type schema:PropertyValue
79 N5cc33212ee6247e29556e1e5a00359ab schema:name pubmed_id
80 schema:value 30190504
81 rdf:type schema:PropertyValue
82 N6bcb2e9f8e5a4cada4904796e3bb0871 rdf:first sg:person.01033506033.12
83 rdf:rest Nd5d3a846bdb54bb680117a27615a4895
84 N76691f989aca4c40898045bc30575967 schema:name Springer Nature - SN SciGraph project
85 rdf:type schema:Organization
86 N9eb3e5d646164bdc90a562a997c7f663 schema:name Aix Marseille Univ., IRD, MEPHI, IHU Méditerranée Infection, Marseille, France
87 rdf:type schema:Organization
88 Naea5744b657a4f758a20dc10f118ed5d schema:issueNumber 1
89 rdf:type schema:PublicationIssue
90 Naf649ceb82884d35aa018458aa4bc169 schema:name Aix Marseille Univ., IRD, MEPHI, IHU Méditerranée Infection, Marseille, France
91 rdf:type schema:Organization
92 Naf7a04c9a39045afb166cad636f49279 schema:name doi
93 schema:value 10.1038/s41598-018-31667-0
94 rdf:type schema:PropertyValue
95 Nbf10722e10fb405297368b2e89963203 schema:volumeNumber 8
96 rdf:type schema:PublicationVolume
97 Nd5d3a846bdb54bb680117a27615a4895 rdf:first sg:person.01106456741.46
98 rdf:rest rdf:nil
99 Ne471a0e0e8754808acb3001bb89e1a31 schema:affiliation N9eb3e5d646164bdc90a562a997c7f663
100 schema:familyName Kaboré
101 schema:givenName Odilon D.
102 rdf:type schema:Person
103 Ne6d7b9326f31478192ecff17a5e5b39a schema:name dimensions_id
104 schema:value pub.1106476140
105 rdf:type schema:PropertyValue
106 Neb9ebd391bba49fbb1babd95dadc7b68 rdf:first Ne471a0e0e8754808acb3001bb89e1a31
107 rdf:rest N0f5fc546a83a42a1b478cb273f5a2224
108 Nfa8ada5bc4464e158e3c3cf84594bf84 schema:name Aix Marseille Univ., IRD, MEPHI, IHU Méditerranée Infection, Marseille, France
109 rdf:type schema:Organization
110 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
111 schema:name Biological Sciences
112 rdf:type schema:DefinedTerm
113 anzsrc-for:0605 schema:inDefinedTermSet anzsrc-for:
114 schema:name Microbiology
115 rdf:type schema:DefinedTerm
116 sg:journal.1045337 schema:issn 2045-2322
117 schema:name Scientific Reports
118 rdf:type schema:Periodical
119 sg:person.01033506033.12 schema:affiliation https://www.grid.ac/institutes/grid.121334.6
120 schema:familyName Godreuil
121 schema:givenName Sylvain
122 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01033506033.12
123 rdf:type schema:Person
124 sg:person.011045203524.70 schema:affiliation Nfa8ada5bc4464e158e3c3cf84594bf84
125 schema:familyName Loukil
126 schema:givenName Ahmed
127 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011045203524.70
128 rdf:type schema:Person
129 sg:person.01106456741.46 schema:affiliation Naf649ceb82884d35aa018458aa4bc169
130 schema:familyName Drancourt
131 schema:givenName Michel
132 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01106456741.46
133 rdf:type schema:Person
134 sg:pub.10.1007/bf02342136 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041742554
135 https://doi.org/10.1007/bf02342136
136 rdf:type schema:CreativeWork
137 sg:pub.10.1007/s00436-014-3976-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048766059
138 https://doi.org/10.1007/s00436-014-3976-8
139 rdf:type schema:CreativeWork
140 sg:pub.10.1007/s00436-016-5271-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012057373
141 https://doi.org/10.1007/s00436-016-5271-3
142 rdf:type schema:CreativeWork
143 sg:pub.10.1007/s10482-013-0003-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023333963
144 https://doi.org/10.1007/s10482-013-0003-5
145 rdf:type schema:CreativeWork
146 sg:pub.10.1038/nrmicro2578 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001868665
147 https://doi.org/10.1038/nrmicro2578
148 rdf:type schema:CreativeWork
149 sg:pub.10.1186/s13062-015-0041-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034470611
150 https://doi.org/10.1186/s13062-015-0041-1
151 rdf:type schema:CreativeWork
152 sg:pub.10.1186/s40793-015-0103-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006573317
153 https://doi.org/10.1186/s40793-015-0103-0
154 rdf:type schema:CreativeWork
155 https://app.dimensions.ai/details/publication/pub.1082060301 schema:CreativeWork
156 https://app.dimensions.ai/details/publication/pub.1083024132 schema:CreativeWork
157 https://doi.org/10.1002/ijc.2910260208 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048280380
158 rdf:type schema:CreativeWork
159 https://doi.org/10.1016/j.copbio.2006.05.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004611689
160 rdf:type schema:CreativeWork
161 https://doi.org/10.1016/j.ijantimicag.2012.10.012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004055102
162 rdf:type schema:CreativeWork
163 https://doi.org/10.1016/j.micinf.2016.12.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007415728
164 rdf:type schema:CreativeWork
165 https://doi.org/10.1016/s0723-2020(11)80042-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022410864
166 rdf:type schema:CreativeWork
167 https://doi.org/10.1021/es0604257 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055499169
168 rdf:type schema:CreativeWork
169 https://doi.org/10.1046/j.1469-0691.2001.00193.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1017593178
170 rdf:type schema:CreativeWork
171 https://doi.org/10.1086/432487 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046307325
172 rdf:type schema:CreativeWork
173 https://doi.org/10.1093/jac/dkq290 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042238044
174 rdf:type schema:CreativeWork
175 https://doi.org/10.1099/13500872-141-7-1493 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060380874
176 rdf:type schema:CreativeWork
177 https://doi.org/10.1111/1574-6941.12258 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033003140
178 rdf:type schema:CreativeWork
179 https://doi.org/10.1111/apm.12087 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034657304
180 rdf:type schema:CreativeWork
181 https://doi.org/10.1111/j.1469-0691.2009.03011.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1051094989
182 rdf:type schema:CreativeWork
183 https://doi.org/10.1111/j.1574-6941.2011.01168.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1013545521
184 rdf:type schema:CreativeWork
185 https://doi.org/10.1111/j.1749-6632.2003.tb07437.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1020652547
186 rdf:type schema:CreativeWork
187 https://doi.org/10.1111/j.1758-2229.2010.00176.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1002299152
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1128/aem.00593-17 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085292782
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1128/aem.00699-11 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030274682
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1128/aem.01332-08 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028485810
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1128/aem.03765-12 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004975063
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1128/aem.68.1.417-422.2002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035349305
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1128/aem.69.7.4316-4319.2003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023688584
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1128/aem.70.9.5094-5101.2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044239623
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1128/aem.71.1.20-28.2005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033455941
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1128/aem.72.1.854-859.2006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010760000
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1128/cmr.17.2.413-433.2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036628596
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1128/jcm.01207-14 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015287109
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1128/jcm.42.12.5493-5501.2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033831086
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1136/jcp.33.12.1179 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039335442
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1371/journal.pone.0029833 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001443274
216 rdf:type schema:CreativeWork
217 https://doi.org/10.2217/fmb-2015-0001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040167149
218 rdf:type schema:CreativeWork
219 https://doi.org/10.3354/ame033239 schema:sameAs https://app.dimensions.ai/details/publication/pub.1071157860
220 rdf:type schema:CreativeWork
221 https://doi.org/10.3389/fmicb.2012.00405 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029054147
222 rdf:type schema:CreativeWork
223 https://www.grid.ac/institutes/grid.121334.6 schema:alternateName University of Montpellier
224 schema:name Université de Montpellier UMR 1058 UMR MIVEGEC, UMR IRD 224-CNRS Inserm, 1058, Montpellier, France
225 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...