Biochemical analyses reveal amino acid residues critical for cell cycle-dependent phosphorylation of human Cdc14A phosphatase by cyclin-dependent kinase 1 View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-08-08

AUTHORS

Sara Ovejero, Patricia Ayala, Marcos Malumbres, Felipe X. Pimentel-Muiños, Avelino Bueno, María P. Sacristán

ABSTRACT

Cdc14 enzymes compose a family of highly conserved phosphatases that are present in a wide range of organisms, including yeast and humans, and that preferentially reverse the phosphorylation of Cyclin-Dependent Kinase (Cdk) substrates. The budding yeast Cdc14 orthologue has essential functions in the control of late mitosis and cytokinesis. In mammals, however, the two Cdc14 homologues, Cdc14A and Cdc14B, do not play a prominent role in controlling late mitotic events, suggesting that some Cdc14 functions are not conserved across species. Moreover, in yeast, Cdc14 is regulated by changes in its subcellular location and by phosphorylation events. In contrast, little is known about the regulation of human Cdc14 phosphatases. Here, we have studied how the human Cdc14A orthologue is regulated during the cell cycle. We found that Cdc14A is phosphorylated on Ser411, Ser453 and Ser549 by Cdk1 early in mitosis and becomes dephosphorylated during late mitotic stages. Interestingly, in vivo and in vitro experiments revealed that, unlike in yeast, Cdk1-mediated phosphorylation of human Cdc14A did not control its catalytic activity but likely modulated its interaction with other proteins in early mitosis. These findings point to differences in Cdk1-mediated mechanisms of regulation between human and yeast Cdc14 orthologues. More... »

PAGES

11871

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-018-30253-8

DOI

http://dx.doi.org/10.1038/s41598-018-30253-8

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1105982861

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30089874


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acids", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biochemical Phenomena", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "CDC2 Protein Kinase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Cycle", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Cycle Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line, Tumor", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cytokinesis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fungal Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "HEK293 Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "HeLa Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mitosis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phosphoric Monoester Hydrolases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phosphorylation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Tyrosine Phosphatases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Yeasts", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Present Address: Institute of Human Genetics, CNRS, Universit\u00e9 de Montpellier, Montpellier, France", 
          "id": "http://www.grid.ac/institutes/grid.121334.6", 
          "name": [
            "Instituto de Biolog\u00eda Molecular y Celular del C\u00e1ncer (IBMCC), Universidad de Salamanca-CSIC, Campus Miguel de Unamuno, 37007 Salamanca, Spain", 
            "Present Address: Institute of Human Genetics, CNRS, Universit\u00e9 de Montpellier, Montpellier, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ovejero", 
        "givenName": "Sara", 
        "id": "sg:person.0755220460.95", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0755220460.95"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Instituto de Biolog\u00eda Molecular y Celular del C\u00e1ncer (IBMCC), Universidad de Salamanca-CSIC, Campus Miguel de Unamuno, 37007 Salamanca, Spain", 
          "id": "http://www.grid.ac/institutes/grid.428472.f", 
          "name": [
            "Instituto de Biolog\u00eda Molecular y Celular del C\u00e1ncer (IBMCC), Universidad de Salamanca-CSIC, Campus Miguel de Unamuno, 37007 Salamanca, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ayala", 
        "givenName": "Patricia", 
        "id": "sg:person.01023333660.31", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01023333660.31"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Centro Nacional de Investigaciones Oncol\u00f3gicas (CNIO), E-28029 Madrid, Spain", 
          "id": "http://www.grid.ac/institutes/grid.7719.8", 
          "name": [
            "Centro Nacional de Investigaciones Oncol\u00f3gicas (CNIO), E-28029 Madrid, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Malumbres", 
        "givenName": "Marcos", 
        "id": "sg:person.0716321250.19", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0716321250.19"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Instituto de Biolog\u00eda Molecular y Celular del C\u00e1ncer (IBMCC), Universidad de Salamanca-CSIC, Campus Miguel de Unamuno, 37007 Salamanca, Spain", 
          "id": "http://www.grid.ac/institutes/grid.428472.f", 
          "name": [
            "Instituto de Biolog\u00eda Molecular y Celular del C\u00e1ncer (IBMCC), Universidad de Salamanca-CSIC, Campus Miguel de Unamuno, 37007 Salamanca, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pimentel-Mui\u00f1os", 
        "givenName": "Felipe X.", 
        "id": "sg:person.0634423732.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0634423732.07"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Departamento de Microbiolog\u00eda y Gen\u00e9tica, Universidad de Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, Spain", 
          "id": "http://www.grid.ac/institutes/grid.11762.33", 
          "name": [
            "Instituto de Biolog\u00eda Molecular y Celular del C\u00e1ncer (IBMCC), Universidad de Salamanca-CSIC, Campus Miguel de Unamuno, 37007 Salamanca, Spain", 
            "Departamento de Microbiolog\u00eda y Gen\u00e9tica, Universidad de Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bueno", 
        "givenName": "Avelino", 
        "id": "sg:person.01304046071.63", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01304046071.63"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Departamento de Microbiolog\u00eda y Gen\u00e9tica, Universidad de Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, Spain", 
          "id": "http://www.grid.ac/institutes/grid.11762.33", 
          "name": [
            "Instituto de Biolog\u00eda Molecular y Celular del C\u00e1ncer (IBMCC), Universidad de Salamanca-CSIC, Campus Miguel de Unamuno, 37007 Salamanca, Spain", 
            "Departamento de Microbiolog\u00eda y Gen\u00e9tica, Universidad de Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sacrist\u00e1n", 
        "givenName": "Mar\u00eda P.", 
        "id": "sg:person.01137562260.56", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01137562260.56"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/srep00189", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047028605", 
          "https://doi.org/10.1038/srep00189"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb2092", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024640130", 
          "https://doi.org/10.1038/ncb2092"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb777", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000490799", 
          "https://doi.org/10.1038/ncb777"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2121-5-27", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007368139", 
          "https://doi.org/10.1186/1471-2121-5-27"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb2365", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013108375", 
          "https://doi.org/10.1038/ncb2365"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncomms10215", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006195590", 
          "https://doi.org/10.1038/ncomms10215"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2018-08-08", 
    "datePublishedReg": "2018-08-08", 
    "description": "Cdc14 enzymes compose a family of highly conserved phosphatases that are present in a wide range of organisms, including yeast and humans, and that preferentially reverse the phosphorylation of Cyclin-Dependent Kinase (Cdk) substrates. The budding yeast Cdc14 orthologue has essential functions in the control of late mitosis and cytokinesis. In mammals, however, the two Cdc14 homologues, Cdc14A and Cdc14B, do not play a prominent role in controlling late mitotic events, suggesting that some Cdc14 functions are not conserved across species. Moreover, in yeast, Cdc14 is regulated by changes in its subcellular location and by phosphorylation events. In contrast, little is known about the regulation of human Cdc14 phosphatases. Here, we have studied how the human Cdc14A orthologue is regulated during the cell cycle. We found that Cdc14A is phosphorylated on Ser411, Ser453 and Ser549 by Cdk1 early in mitosis and becomes dephosphorylated during late mitotic stages. Interestingly, in vivo and in vitro experiments revealed that, unlike in yeast, Cdk1-mediated phosphorylation of human Cdc14A did not control its catalytic activity but likely modulated its interaction with other proteins in early mitosis. These findings point to differences in Cdk1-mediated mechanisms of regulation between human and yeast Cdc14 orthologues.", 
    "genre": "article", 
    "id": "sg:pub.10.1038/s41598-018-30253-8", 
    "inLanguage": "en", 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1045337", 
        "issn": [
          "2045-2322"
        ], 
        "name": "Scientific Reports", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "8"
      }
    ], 
    "keywords": [
      "cell cycle-dependent phosphorylation", 
      "late mitotic events", 
      "cyclin-dependent kinase 1", 
      "late mitotic stages", 
      "cyclin-dependent kinases", 
      "mechanism of regulation", 
      "amino acid residues", 
      "human Cdc14", 
      "yeast Cdc14", 
      "Cdc14A phosphatase", 
      "human Cdc14A", 
      "phosphorylation events", 
      "late mitosis", 
      "Cdc14 function", 
      "Cdc14", 
      "subcellular location", 
      "early mitosis", 
      "mitotic events", 
      "essential functions", 
      "Cdc14A", 
      "kinase 1", 
      "cell cycle", 
      "mitotic stages", 
      "acid residues", 
      "CDK1", 
      "mitosis", 
      "yeast", 
      "phosphorylation", 
      "orthologues", 
      "biochemical analysis", 
      "phosphatase", 
      "regulation", 
      "Cdc14B", 
      "Ser453", 
      "cytokinesis", 
      "Ser549", 
      "Ser411", 
      "mammals", 
      "homologues", 
      "kinase", 
      "prominent role", 
      "organisms", 
      "species", 
      "protein", 
      "residues", 
      "humans", 
      "catalytic activity", 
      "family", 
      "vivo", 
      "function", 
      "wide range", 
      "events", 
      "mechanism", 
      "role", 
      "interaction", 
      "activity", 
      "cycle", 
      "contrast", 
      "stage", 
      "changes", 
      "analysis", 
      "location", 
      "experiments", 
      "control", 
      "findings", 
      "differences", 
      "range", 
      "yeast Cdc14 orthologue", 
      "Cdc14 orthologue", 
      "Cdc14 homologues", 
      "human Cdc14A orthologue", 
      "Cdc14A orthologue", 
      "cycle-dependent phosphorylation", 
      "human Cdc14A phosphatase"
    ], 
    "name": "Biochemical analyses reveal amino acid residues critical for cell cycle-dependent phosphorylation of human Cdc14A phosphatase by cyclin-dependent kinase 1", 
    "pagination": "11871", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1105982861"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/s41598-018-30253-8"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30089874"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/s41598-018-30253-8", 
      "https://app.dimensions.ai/details/publication/pub.1105982861"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-01-01T18:50", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_784.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1038/s41598-018-30253-8"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-30253-8'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-30253-8'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-30253-8'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-30253-8'


 

This table displays all metadata directly associated to this object as RDF triples.

273 TRIPLES      22 PREDICATES      123 URIs      109 LITERALS      24 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/s41598-018-30253-8 schema:about N15554423fa984b5ebb8cd8380bcd78b3
2 N17c743c3aa4b482e8cc3e294c0931a44
3 N1bf6c0b31bbe4a2a818beaa0ce6ec559
4 N2d625c7269f24d838b1286844955465a
5 N2e2309a8b6b04437acaec5281d610a1c
6 N313de25435a44d8d8f7fd4202d8c7df3
7 N4dffa57a5648463ea2661a1a2a7f4a2b
8 N5ed366f592ae484888597688e032ee88
9 N6b3be52dc4e24b0a8cce99907c83614f
10 N82f87447cb484e2a885663645ffe70e3
11 N89c975143c0a4bd681a6e1021b2137a1
12 Na05ead07db4e4bc48522e2642d9e41f6
13 Nb238fd9c83be4e7fa050194aabbd6696
14 Nca3baa81e9e24308889aea827180810a
15 Ndab2dfd7388146be9f364af3d69c0bf4
16 Ndf55a0e770ac49aabe3e785da63591bb
17 Nf896c1def8c548d386f3c619a45858b4
18 anzsrc-for:06
19 anzsrc-for:0601
20 schema:author N2ebda76059e34ba28c0413a560f5affc
21 schema:citation sg:pub.10.1038/ncb2092
22 sg:pub.10.1038/ncb2365
23 sg:pub.10.1038/ncb777
24 sg:pub.10.1038/ncomms10215
25 sg:pub.10.1038/srep00189
26 sg:pub.10.1186/1471-2121-5-27
27 schema:datePublished 2018-08-08
28 schema:datePublishedReg 2018-08-08
29 schema:description Cdc14 enzymes compose a family of highly conserved phosphatases that are present in a wide range of organisms, including yeast and humans, and that preferentially reverse the phosphorylation of Cyclin-Dependent Kinase (Cdk) substrates. The budding yeast Cdc14 orthologue has essential functions in the control of late mitosis and cytokinesis. In mammals, however, the two Cdc14 homologues, Cdc14A and Cdc14B, do not play a prominent role in controlling late mitotic events, suggesting that some Cdc14 functions are not conserved across species. Moreover, in yeast, Cdc14 is regulated by changes in its subcellular location and by phosphorylation events. In contrast, little is known about the regulation of human Cdc14 phosphatases. Here, we have studied how the human Cdc14A orthologue is regulated during the cell cycle. We found that Cdc14A is phosphorylated on Ser411, Ser453 and Ser549 by Cdk1 early in mitosis and becomes dephosphorylated during late mitotic stages. Interestingly, in vivo and in vitro experiments revealed that, unlike in yeast, Cdk1-mediated phosphorylation of human Cdc14A did not control its catalytic activity but likely modulated its interaction with other proteins in early mitosis. These findings point to differences in Cdk1-mediated mechanisms of regulation between human and yeast Cdc14 orthologues.
30 schema:genre article
31 schema:inLanguage en
32 schema:isAccessibleForFree true
33 schema:isPartOf N5ad8e674fc8e46d19717645953d54e29
34 N866caabcc02e4f7b8ad6f7d740f9379e
35 sg:journal.1045337
36 schema:keywords CDK1
37 Cdc14
38 Cdc14 function
39 Cdc14 homologues
40 Cdc14 orthologue
41 Cdc14A
42 Cdc14A orthologue
43 Cdc14A phosphatase
44 Cdc14B
45 Ser411
46 Ser453
47 Ser549
48 acid residues
49 activity
50 amino acid residues
51 analysis
52 biochemical analysis
53 catalytic activity
54 cell cycle
55 cell cycle-dependent phosphorylation
56 changes
57 contrast
58 control
59 cycle
60 cycle-dependent phosphorylation
61 cyclin-dependent kinase 1
62 cyclin-dependent kinases
63 cytokinesis
64 differences
65 early mitosis
66 essential functions
67 events
68 experiments
69 family
70 findings
71 function
72 homologues
73 human Cdc14
74 human Cdc14A
75 human Cdc14A orthologue
76 human Cdc14A phosphatase
77 humans
78 interaction
79 kinase
80 kinase 1
81 late mitosis
82 late mitotic events
83 late mitotic stages
84 location
85 mammals
86 mechanism
87 mechanism of regulation
88 mitosis
89 mitotic events
90 mitotic stages
91 organisms
92 orthologues
93 phosphatase
94 phosphorylation
95 phosphorylation events
96 prominent role
97 protein
98 range
99 regulation
100 residues
101 role
102 species
103 stage
104 subcellular location
105 vivo
106 wide range
107 yeast
108 yeast Cdc14
109 yeast Cdc14 orthologue
110 schema:name Biochemical analyses reveal amino acid residues critical for cell cycle-dependent phosphorylation of human Cdc14A phosphatase by cyclin-dependent kinase 1
111 schema:pagination 11871
112 schema:productId N259bb85f9bec4648a0f0212f75c8ca7b
113 N38832f40b78f49369b32972c67d365e7
114 N54da24a0f4a34a6baa49f5aed5d9113a
115 schema:sameAs https://app.dimensions.ai/details/publication/pub.1105982861
116 https://doi.org/10.1038/s41598-018-30253-8
117 schema:sdDatePublished 2022-01-01T18:50
118 schema:sdLicense https://scigraph.springernature.com/explorer/license/
119 schema:sdPublisher Na59ec933551243a4a836491b4f9a198d
120 schema:url https://doi.org/10.1038/s41598-018-30253-8
121 sgo:license sg:explorer/license/
122 sgo:sdDataset articles
123 rdf:type schema:ScholarlyArticle
124 N15554423fa984b5ebb8cd8380bcd78b3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Cell Cycle
126 rdf:type schema:DefinedTerm
127 N17c743c3aa4b482e8cc3e294c0931a44 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name Biochemical Phenomena
129 rdf:type schema:DefinedTerm
130 N1bf6c0b31bbe4a2a818beaa0ce6ec559 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Yeasts
132 rdf:type schema:DefinedTerm
133 N249bd9834baa4b809e2ca755d16404bd rdf:first sg:person.01137562260.56
134 rdf:rest rdf:nil
135 N259bb85f9bec4648a0f0212f75c8ca7b schema:name doi
136 schema:value 10.1038/s41598-018-30253-8
137 rdf:type schema:PropertyValue
138 N2d625c7269f24d838b1286844955465a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
139 schema:name HeLa Cells
140 rdf:type schema:DefinedTerm
141 N2e2309a8b6b04437acaec5281d610a1c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name Phosphoric Monoester Hydrolases
143 rdf:type schema:DefinedTerm
144 N2ebda76059e34ba28c0413a560f5affc rdf:first sg:person.0755220460.95
145 rdf:rest Ncad60cd525794014bdb99b25efd543c4
146 N313de25435a44d8d8f7fd4202d8c7df3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Fungal Proteins
148 rdf:type schema:DefinedTerm
149 N38832f40b78f49369b32972c67d365e7 schema:name dimensions_id
150 schema:value pub.1105982861
151 rdf:type schema:PropertyValue
152 N4528220c9178499fa519c1fe72743041 rdf:first sg:person.0634423732.07
153 rdf:rest N59ee98a2f7ed413a9d9ee73bfade4f7f
154 N4dffa57a5648463ea2661a1a2a7f4a2b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
155 schema:name Phosphorylation
156 rdf:type schema:DefinedTerm
157 N54da24a0f4a34a6baa49f5aed5d9113a schema:name pubmed_id
158 schema:value 30089874
159 rdf:type schema:PropertyValue
160 N59ee98a2f7ed413a9d9ee73bfade4f7f rdf:first sg:person.01304046071.63
161 rdf:rest N249bd9834baa4b809e2ca755d16404bd
162 N5ad8e674fc8e46d19717645953d54e29 schema:volumeNumber 8
163 rdf:type schema:PublicationVolume
164 N5ed366f592ae484888597688e032ee88 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
165 schema:name Protein Tyrosine Phosphatases
166 rdf:type schema:DefinedTerm
167 N6b3be52dc4e24b0a8cce99907c83614f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
168 schema:name Mitosis
169 rdf:type schema:DefinedTerm
170 N82f87447cb484e2a885663645ffe70e3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
171 schema:name Cell Line, Tumor
172 rdf:type schema:DefinedTerm
173 N866caabcc02e4f7b8ad6f7d740f9379e schema:issueNumber 1
174 rdf:type schema:PublicationIssue
175 N89c975143c0a4bd681a6e1021b2137a1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
176 schema:name Humans
177 rdf:type schema:DefinedTerm
178 Na05ead07db4e4bc48522e2642d9e41f6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
179 schema:name Amino Acids
180 rdf:type schema:DefinedTerm
181 Na59ec933551243a4a836491b4f9a198d schema:name Springer Nature - SN SciGraph project
182 rdf:type schema:Organization
183 Nb238fd9c83be4e7fa050194aabbd6696 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
184 schema:name Cytokinesis
185 rdf:type schema:DefinedTerm
186 Nca3baa81e9e24308889aea827180810a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
187 schema:name HEK293 Cells
188 rdf:type schema:DefinedTerm
189 Ncad60cd525794014bdb99b25efd543c4 rdf:first sg:person.01023333660.31
190 rdf:rest Ne039282344da4290ab4a69c42a5235c5
191 Ndab2dfd7388146be9f364af3d69c0bf4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
192 schema:name CDC2 Protein Kinase
193 rdf:type schema:DefinedTerm
194 Ndf55a0e770ac49aabe3e785da63591bb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
195 schema:name Cell Line
196 rdf:type schema:DefinedTerm
197 Ne039282344da4290ab4a69c42a5235c5 rdf:first sg:person.0716321250.19
198 rdf:rest N4528220c9178499fa519c1fe72743041
199 Nf896c1def8c548d386f3c619a45858b4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
200 schema:name Cell Cycle Proteins
201 rdf:type schema:DefinedTerm
202 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
203 schema:name Biological Sciences
204 rdf:type schema:DefinedTerm
205 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
206 schema:name Biochemistry and Cell Biology
207 rdf:type schema:DefinedTerm
208 sg:journal.1045337 schema:issn 2045-2322
209 schema:name Scientific Reports
210 schema:publisher Springer Nature
211 rdf:type schema:Periodical
212 sg:person.01023333660.31 schema:affiliation grid-institutes:grid.428472.f
213 schema:familyName Ayala
214 schema:givenName Patricia
215 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01023333660.31
216 rdf:type schema:Person
217 sg:person.01137562260.56 schema:affiliation grid-institutes:grid.11762.33
218 schema:familyName Sacristán
219 schema:givenName María P.
220 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01137562260.56
221 rdf:type schema:Person
222 sg:person.01304046071.63 schema:affiliation grid-institutes:grid.11762.33
223 schema:familyName Bueno
224 schema:givenName Avelino
225 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01304046071.63
226 rdf:type schema:Person
227 sg:person.0634423732.07 schema:affiliation grid-institutes:grid.428472.f
228 schema:familyName Pimentel-Muiños
229 schema:givenName Felipe X.
230 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0634423732.07
231 rdf:type schema:Person
232 sg:person.0716321250.19 schema:affiliation grid-institutes:grid.7719.8
233 schema:familyName Malumbres
234 schema:givenName Marcos
235 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0716321250.19
236 rdf:type schema:Person
237 sg:person.0755220460.95 schema:affiliation grid-institutes:grid.121334.6
238 schema:familyName Ovejero
239 schema:givenName Sara
240 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0755220460.95
241 rdf:type schema:Person
242 sg:pub.10.1038/ncb2092 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024640130
243 https://doi.org/10.1038/ncb2092
244 rdf:type schema:CreativeWork
245 sg:pub.10.1038/ncb2365 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013108375
246 https://doi.org/10.1038/ncb2365
247 rdf:type schema:CreativeWork
248 sg:pub.10.1038/ncb777 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000490799
249 https://doi.org/10.1038/ncb777
250 rdf:type schema:CreativeWork
251 sg:pub.10.1038/ncomms10215 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006195590
252 https://doi.org/10.1038/ncomms10215
253 rdf:type schema:CreativeWork
254 sg:pub.10.1038/srep00189 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047028605
255 https://doi.org/10.1038/srep00189
256 rdf:type schema:CreativeWork
257 sg:pub.10.1186/1471-2121-5-27 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007368139
258 https://doi.org/10.1186/1471-2121-5-27
259 rdf:type schema:CreativeWork
260 grid-institutes:grid.11762.33 schema:alternateName Departamento de Microbiología y Genética, Universidad de Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, Spain
261 schema:name Departamento de Microbiología y Genética, Universidad de Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, Spain
262 Instituto de Biología Molecular y Celular del Cáncer (IBMCC), Universidad de Salamanca-CSIC, Campus Miguel de Unamuno, 37007 Salamanca, Spain
263 rdf:type schema:Organization
264 grid-institutes:grid.121334.6 schema:alternateName Present Address: Institute of Human Genetics, CNRS, Université de Montpellier, Montpellier, France
265 schema:name Instituto de Biología Molecular y Celular del Cáncer (IBMCC), Universidad de Salamanca-CSIC, Campus Miguel de Unamuno, 37007 Salamanca, Spain
266 Present Address: Institute of Human Genetics, CNRS, Université de Montpellier, Montpellier, France
267 rdf:type schema:Organization
268 grid-institutes:grid.428472.f schema:alternateName Instituto de Biología Molecular y Celular del Cáncer (IBMCC), Universidad de Salamanca-CSIC, Campus Miguel de Unamuno, 37007 Salamanca, Spain
269 schema:name Instituto de Biología Molecular y Celular del Cáncer (IBMCC), Universidad de Salamanca-CSIC, Campus Miguel de Unamuno, 37007 Salamanca, Spain
270 rdf:type schema:Organization
271 grid-institutes:grid.7719.8 schema:alternateName Centro Nacional de Investigaciones Oncológicas (CNIO), E-28029 Madrid, Spain
272 schema:name Centro Nacional de Investigaciones Oncológicas (CNIO), E-28029 Madrid, Spain
273 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...