PLVAP and GKN3 Are Two Critical Host Cell Receptors Which Facilitate Japanese Encephalitis Virus Entry Into Neurons View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-12

AUTHORS

Sriparna Mukherjee, Nabonita Sengupta, Ankur Chaudhuri, Irshad Akbar, Noopur Singh, Sibani Chakraborty, Amol Ratnakar Suryawanshi, Arindam Bhattacharyya, Anirban Basu

ABSTRACT

Japanese Encephalitis Virus (JEV), a globally important pathogen, belongs to the family Flaviviridae, is transmitted between vertebrate hosts by mosquitoes, principally by Culex tritaeniorhynchus. The E-glycoprotein of the virus mediates its attachment to the host cell receptors. In this study, we cloned and purified JEV E-glycoprotein in pET28a vector using E. coli BL21 (DE3) cells. A pull down assay was performed using plasma membrane fraction of BALB/c mouse brain and E-glycoprotein as a bait protein. 2-Dimensional Gel Electrophoresis based separation of the interacting proteins was analyzed by mass spectrometry. Among all the identified partners of E-glycoprotein, PLVAP (Plasmalemma vesicle associated protein) and GKN3 (Gastrokine3) showed significant up-regulation in both JEV infected mouse brain and neuro2a cells. In-silico studies also predicted significant interaction of these receptors with E-glycoprotein. Additionally, overexperssion and silencing of these receptors resulted in increase and reduction in viral load respectively, suggesting them as two critical cellular receptors governing JEV entry and propagation in neurons. In support, we observed significant expression of PLVAP but not GKN3 in post-mortem autopsied human brain tissue. Our results establish two novel receptor proteins in neurons in case of JEV infection, thus providing potential targets for antiviral research. More... »

PAGES

11784

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-018-30054-z

DOI

http://dx.doi.org/10.1038/s41598-018-30054-z

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1105928938

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30082709


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Calcutta", 
          "id": "https://www.grid.ac/institutes/grid.59056.3f", 
          "name": [
            "National Brain Research Centre, 122052, Manesar, Haryana, India", 
            "Immunology Lab, Department of Zoology, University of Calcutta, 35, Ballygunge Circular Road, 700019, Kolkata, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mukherjee", 
        "givenName": "Sriparna", 
        "id": "sg:person.0605761546.99", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0605761546.99"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Indian Institute of Science Bangalore", 
          "id": "https://www.grid.ac/institutes/grid.34980.36", 
          "name": [
            "National Brain Research Centre, 122052, Manesar, Haryana, India", 
            "Microbiology and Cell Biology, Indian Institute of Science, CV Raman Avenue, 560012, Bangalore, Karnataka, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sengupta", 
        "givenName": "Nabonita", 
        "id": "sg:person.01345457340.13", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01345457340.13"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "West Bengal State University", 
          "id": "https://www.grid.ac/institutes/grid.419478.7", 
          "name": [
            "West Bengal State University, North 24 Parganas, Barasat, 700126, Kolkata, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chaudhuri", 
        "givenName": "Ankur", 
        "id": "sg:person.01152234460.59", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01152234460.59"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Brain Research Centre", 
          "id": "https://www.grid.ac/institutes/grid.250277.5", 
          "name": [
            "National Brain Research Centre, 122052, Manesar, Haryana, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Akbar", 
        "givenName": "Irshad", 
        "id": "sg:person.013667404666.56", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013667404666.56"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Brain Research Centre", 
          "id": "https://www.grid.ac/institutes/grid.250277.5", 
          "name": [
            "National Brain Research Centre, 122052, Manesar, Haryana, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Singh", 
        "givenName": "Noopur", 
        "id": "sg:person.015712361313.54", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015712361313.54"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "West Bengal State University", 
          "id": "https://www.grid.ac/institutes/grid.419478.7", 
          "name": [
            "West Bengal State University, North 24 Parganas, Barasat, 700126, Kolkata, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chakraborty", 
        "givenName": "Sibani", 
        "id": "sg:person.01266463060.51", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01266463060.51"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Life Sciences", 
          "id": "https://www.grid.ac/institutes/grid.418782.0", 
          "name": [
            "Clinical Proteomics, Institute of Life Sciences, 751023, Bhubaneswar, Odisha, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Suryawanshi", 
        "givenName": "Amol Ratnakar", 
        "id": "sg:person.0636736703.09", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0636736703.09"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Calcutta", 
          "id": "https://www.grid.ac/institutes/grid.59056.3f", 
          "name": [
            "Immunology Lab, Department of Zoology, University of Calcutta, 35, Ballygunge Circular Road, 700019, Kolkata, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bhattacharyya", 
        "givenName": "Arindam", 
        "id": "sg:person.0765211606.93", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0765211606.93"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Brain Research Centre", 
          "id": "https://www.grid.ac/institutes/grid.250277.5", 
          "name": [
            "National Brain Research Centre, 122052, Manesar, Haryana, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Basu", 
        "givenName": "Anirban", 
        "id": "sg:person.0703222001.31", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0703222001.31"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1002/prot.10389", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000099680"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/ajpgi.00374.2012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001227809"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.06072-11", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002746715"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pntd.0000437", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004390586"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0090211", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010559461"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.01399-12", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011796623"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2202-9-29", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013443095", 
          "https://doi.org/10.1186/1471-2202-9-29"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.biophys.29.1.291", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013615308"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ar000033j", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017409579"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ar000033j", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017409579"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0075188", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022454895"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1053/j.gastro.2010.08.043", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024976051"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/path.1805", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026057429"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/path.1805", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026057429"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.neuint.2009.07.006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026830599"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1053/j.gastro.2010.01.050", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029615264"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jmbi.1993.1626", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030443070"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/pmic.201200227", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030714079"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m410208200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030750438"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/prot.10460", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031248046"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/375291a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032366356", 
          "https://doi.org/10.1038/375291a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/physiolgenomics.00169.2012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033219156"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jprot.2012.01.016", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036218556"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/pmic.201000809", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037114667"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1471-4159.2010.06951.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037576573"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1471-4159.2010.06951.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037576573"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jmv.23248", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040806001"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1743-422x-8-128", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041755117", 
          "https://doi.org/10.1186/1743-422x-8-128"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s12974-016-0486-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042081468", 
          "https://doi.org/10.1186/s12974-016-0486-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.virol.2006.01.022", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045304049"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.virol.2008.10.025", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045956259"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/cddis.2016.394", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046283415", 
          "https://doi.org/10.1038/cddis.2016.394"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1471-4159.2008.05511.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048819927"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.virusres.2011.06.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048986569"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/1097-0142(19931115)72:10<3061::aid-cncr2820721031>3.0.co;2-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051271346"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.75.16.7769-7773.2001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052908790"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/rmv.1868", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053337571"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkh468", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053719590"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/vir.0.034637-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060398857"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/vir.0.18883-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060399805"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.02274-16", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062713038"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2471/blt.10.085233", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070837773"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2018-12", 
    "datePublishedReg": "2018-12-01", 
    "description": "Japanese Encephalitis Virus (JEV), a globally important pathogen, belongs to the family Flaviviridae, is transmitted between vertebrate hosts by mosquitoes, principally by Culex tritaeniorhynchus. The E-glycoprotein of the virus mediates its attachment to the host cell receptors. In this study, we cloned and purified JEV E-glycoprotein in pET28a vector using E. coli BL21 (DE3) cells. A pull down assay was performed using plasma membrane fraction of BALB/c mouse brain and E-glycoprotein as a bait protein. 2-Dimensional Gel Electrophoresis based separation of the interacting proteins was analyzed by mass spectrometry. Among all the identified partners of E-glycoprotein, PLVAP (Plasmalemma vesicle associated protein) and GKN3 (Gastrokine3) showed significant up-regulation in both JEV infected mouse brain and neuro2a cells. In-silico studies also predicted significant interaction of these receptors with E-glycoprotein. Additionally, overexperssion and silencing of these receptors resulted in increase and reduction in viral load respectively, suggesting them as two critical cellular receptors governing JEV entry and propagation in neurons. In support, we observed significant expression of PLVAP but not GKN3 in post-mortem autopsied human brain tissue. Our results establish two novel receptor proteins in neurons in case of JEV infection, thus providing potential targets for antiviral research.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/s41598-018-30054-z", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1045337", 
        "issn": [
          "2045-2322"
        ], 
        "name": "Scientific Reports", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "8"
      }
    ], 
    "name": "PLVAP and GKN3 Are Two Critical Host Cell Receptors Which Facilitate Japanese Encephalitis Virus Entry Into Neurons", 
    "pagination": "11784", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "b1ecc2af6f00d83f0ff58857bdcafa63d10aeec9b4f1072f9d9325a070fcbec3"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30082709"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101563288"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/s41598-018-30054-z"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1105928938"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/s41598-018-30054-z", 
      "https://app.dimensions.ai/details/publication/pub.1105928938"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T20:55", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8684_00000571.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/s41598-018-30054-z"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-30054-z'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-30054-z'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-30054-z'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-30054-z'


 

This table displays all metadata directly associated to this object as RDF triples.

260 TRIPLES      21 PREDICATES      68 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/s41598-018-30054-z schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author Ndc8c8dfcb621443e849296ad227f34cc
4 schema:citation sg:pub.10.1038/375291a0
5 sg:pub.10.1038/cddis.2016.394
6 sg:pub.10.1186/1471-2202-9-29
7 sg:pub.10.1186/1743-422x-8-128
8 sg:pub.10.1186/s12974-016-0486-x
9 https://doi.org/10.1002/1097-0142(19931115)72:10<3061::aid-cncr2820721031>3.0.co;2-6
10 https://doi.org/10.1002/jmv.23248
11 https://doi.org/10.1002/path.1805
12 https://doi.org/10.1002/pmic.201000809
13 https://doi.org/10.1002/pmic.201200227
14 https://doi.org/10.1002/prot.10389
15 https://doi.org/10.1002/prot.10460
16 https://doi.org/10.1002/rmv.1868
17 https://doi.org/10.1006/jmbi.1993.1626
18 https://doi.org/10.1016/j.jprot.2012.01.016
19 https://doi.org/10.1016/j.neuint.2009.07.006
20 https://doi.org/10.1016/j.virol.2006.01.022
21 https://doi.org/10.1016/j.virol.2008.10.025
22 https://doi.org/10.1016/j.virusres.2011.06.008
23 https://doi.org/10.1021/ar000033j
24 https://doi.org/10.1053/j.gastro.2010.01.050
25 https://doi.org/10.1053/j.gastro.2010.08.043
26 https://doi.org/10.1074/jbc.m410208200
27 https://doi.org/10.1093/nar/gkh468
28 https://doi.org/10.1099/vir.0.034637-0
29 https://doi.org/10.1099/vir.0.18883-0
30 https://doi.org/10.1111/j.1471-4159.2008.05511.x
31 https://doi.org/10.1111/j.1471-4159.2010.06951.x
32 https://doi.org/10.1128/jvi.01399-12
33 https://doi.org/10.1128/jvi.02274-16
34 https://doi.org/10.1128/jvi.06072-11
35 https://doi.org/10.1128/jvi.75.16.7769-7773.2001
36 https://doi.org/10.1146/annurev.biophys.29.1.291
37 https://doi.org/10.1152/ajpgi.00374.2012
38 https://doi.org/10.1152/physiolgenomics.00169.2012
39 https://doi.org/10.1371/journal.pntd.0000437
40 https://doi.org/10.1371/journal.pone.0075188
41 https://doi.org/10.1371/journal.pone.0090211
42 https://doi.org/10.2471/blt.10.085233
43 schema:datePublished 2018-12
44 schema:datePublishedReg 2018-12-01
45 schema:description Japanese Encephalitis Virus (JEV), a globally important pathogen, belongs to the family Flaviviridae, is transmitted between vertebrate hosts by mosquitoes, principally by Culex tritaeniorhynchus. The E-glycoprotein of the virus mediates its attachment to the host cell receptors. In this study, we cloned and purified JEV E-glycoprotein in pET28a vector using E. coli BL21 (DE3) cells. A pull down assay was performed using plasma membrane fraction of BALB/c mouse brain and E-glycoprotein as a bait protein. 2-Dimensional Gel Electrophoresis based separation of the interacting proteins was analyzed by mass spectrometry. Among all the identified partners of E-glycoprotein, PLVAP (Plasmalemma vesicle associated protein) and GKN3 (Gastrokine3) showed significant up-regulation in both JEV infected mouse brain and neuro2a cells. In-silico studies also predicted significant interaction of these receptors with E-glycoprotein. Additionally, overexperssion and silencing of these receptors resulted in increase and reduction in viral load respectively, suggesting them as two critical cellular receptors governing JEV entry and propagation in neurons. In support, we observed significant expression of PLVAP but not GKN3 in post-mortem autopsied human brain tissue. Our results establish two novel receptor proteins in neurons in case of JEV infection, thus providing potential targets for antiviral research.
46 schema:genre research_article
47 schema:inLanguage en
48 schema:isAccessibleForFree true
49 schema:isPartOf Ndb37a607163a4809a662307d0edea309
50 Ndd36ee2fddef4d1caa690fc17a39da4f
51 sg:journal.1045337
52 schema:name PLVAP and GKN3 Are Two Critical Host Cell Receptors Which Facilitate Japanese Encephalitis Virus Entry Into Neurons
53 schema:pagination 11784
54 schema:productId N11054c023de94b46aaeced903abfe0a9
55 N735dda63e3ec4ec9a7e2f27524e3c1b9
56 Nadde206aec1b41db94b375859b7ff7d7
57 Naedb07b915de4fd4999ee1b6ca057ebf
58 Nef4a33e8777d43739778582c565e85d5
59 schema:sameAs https://app.dimensions.ai/details/publication/pub.1105928938
60 https://doi.org/10.1038/s41598-018-30054-z
61 schema:sdDatePublished 2019-04-10T20:55
62 schema:sdLicense https://scigraph.springernature.com/explorer/license/
63 schema:sdPublisher N8d6cef192eb1445c8a029f64037b4700
64 schema:url https://www.nature.com/articles/s41598-018-30054-z
65 sgo:license sg:explorer/license/
66 sgo:sdDataset articles
67 rdf:type schema:ScholarlyArticle
68 N11054c023de94b46aaeced903abfe0a9 schema:name dimensions_id
69 schema:value pub.1105928938
70 rdf:type schema:PropertyValue
71 N1ea22403f9d14d7a8b479531de0e63d8 rdf:first sg:person.0765211606.93
72 rdf:rest N21ca026e68ed41709a928a56686ec1f1
73 N21ca026e68ed41709a928a56686ec1f1 rdf:first sg:person.0703222001.31
74 rdf:rest rdf:nil
75 N6c66d975a29345ff903fb2fc5c0e6077 rdf:first sg:person.015712361313.54
76 rdf:rest Nd288eb88fd3f4cddb8afdc0a6f81c7d1
77 N735dda63e3ec4ec9a7e2f27524e3c1b9 schema:name pubmed_id
78 schema:value 30082709
79 rdf:type schema:PropertyValue
80 N82eb83f74cff44c697d5188d4957e1ea rdf:first sg:person.0636736703.09
81 rdf:rest N1ea22403f9d14d7a8b479531de0e63d8
82 N8d6cef192eb1445c8a029f64037b4700 schema:name Springer Nature - SN SciGraph project
83 rdf:type schema:Organization
84 Na77f5330aeee499ea3f1e850b054ba7f rdf:first sg:person.01345457340.13
85 rdf:rest Nbf9974e264484d60a02c87aa481ab354
86 Nadde206aec1b41db94b375859b7ff7d7 schema:name doi
87 schema:value 10.1038/s41598-018-30054-z
88 rdf:type schema:PropertyValue
89 Naedb07b915de4fd4999ee1b6ca057ebf schema:name readcube_id
90 schema:value b1ecc2af6f00d83f0ff58857bdcafa63d10aeec9b4f1072f9d9325a070fcbec3
91 rdf:type schema:PropertyValue
92 Nbf9974e264484d60a02c87aa481ab354 rdf:first sg:person.01152234460.59
93 rdf:rest Ncaba2c40a270456e8612451bfed4ee5f
94 Ncaba2c40a270456e8612451bfed4ee5f rdf:first sg:person.013667404666.56
95 rdf:rest N6c66d975a29345ff903fb2fc5c0e6077
96 Nd288eb88fd3f4cddb8afdc0a6f81c7d1 rdf:first sg:person.01266463060.51
97 rdf:rest N82eb83f74cff44c697d5188d4957e1ea
98 Ndb37a607163a4809a662307d0edea309 schema:issueNumber 1
99 rdf:type schema:PublicationIssue
100 Ndc8c8dfcb621443e849296ad227f34cc rdf:first sg:person.0605761546.99
101 rdf:rest Na77f5330aeee499ea3f1e850b054ba7f
102 Ndd36ee2fddef4d1caa690fc17a39da4f schema:volumeNumber 8
103 rdf:type schema:PublicationVolume
104 Nef4a33e8777d43739778582c565e85d5 schema:name nlm_unique_id
105 schema:value 101563288
106 rdf:type schema:PropertyValue
107 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
108 schema:name Biological Sciences
109 rdf:type schema:DefinedTerm
110 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
111 schema:name Biochemistry and Cell Biology
112 rdf:type schema:DefinedTerm
113 sg:journal.1045337 schema:issn 2045-2322
114 schema:name Scientific Reports
115 rdf:type schema:Periodical
116 sg:person.01152234460.59 schema:affiliation https://www.grid.ac/institutes/grid.419478.7
117 schema:familyName Chaudhuri
118 schema:givenName Ankur
119 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01152234460.59
120 rdf:type schema:Person
121 sg:person.01266463060.51 schema:affiliation https://www.grid.ac/institutes/grid.419478.7
122 schema:familyName Chakraborty
123 schema:givenName Sibani
124 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01266463060.51
125 rdf:type schema:Person
126 sg:person.01345457340.13 schema:affiliation https://www.grid.ac/institutes/grid.34980.36
127 schema:familyName Sengupta
128 schema:givenName Nabonita
129 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01345457340.13
130 rdf:type schema:Person
131 sg:person.013667404666.56 schema:affiliation https://www.grid.ac/institutes/grid.250277.5
132 schema:familyName Akbar
133 schema:givenName Irshad
134 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013667404666.56
135 rdf:type schema:Person
136 sg:person.015712361313.54 schema:affiliation https://www.grid.ac/institutes/grid.250277.5
137 schema:familyName Singh
138 schema:givenName Noopur
139 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015712361313.54
140 rdf:type schema:Person
141 sg:person.0605761546.99 schema:affiliation https://www.grid.ac/institutes/grid.59056.3f
142 schema:familyName Mukherjee
143 schema:givenName Sriparna
144 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0605761546.99
145 rdf:type schema:Person
146 sg:person.0636736703.09 schema:affiliation https://www.grid.ac/institutes/grid.418782.0
147 schema:familyName Suryawanshi
148 schema:givenName Amol Ratnakar
149 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0636736703.09
150 rdf:type schema:Person
151 sg:person.0703222001.31 schema:affiliation https://www.grid.ac/institutes/grid.250277.5
152 schema:familyName Basu
153 schema:givenName Anirban
154 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0703222001.31
155 rdf:type schema:Person
156 sg:person.0765211606.93 schema:affiliation https://www.grid.ac/institutes/grid.59056.3f
157 schema:familyName Bhattacharyya
158 schema:givenName Arindam
159 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0765211606.93
160 rdf:type schema:Person
161 sg:pub.10.1038/375291a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032366356
162 https://doi.org/10.1038/375291a0
163 rdf:type schema:CreativeWork
164 sg:pub.10.1038/cddis.2016.394 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046283415
165 https://doi.org/10.1038/cddis.2016.394
166 rdf:type schema:CreativeWork
167 sg:pub.10.1186/1471-2202-9-29 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013443095
168 https://doi.org/10.1186/1471-2202-9-29
169 rdf:type schema:CreativeWork
170 sg:pub.10.1186/1743-422x-8-128 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041755117
171 https://doi.org/10.1186/1743-422x-8-128
172 rdf:type schema:CreativeWork
173 sg:pub.10.1186/s12974-016-0486-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1042081468
174 https://doi.org/10.1186/s12974-016-0486-x
175 rdf:type schema:CreativeWork
176 https://doi.org/10.1002/1097-0142(19931115)72:10<3061::aid-cncr2820721031>3.0.co;2-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051271346
177 rdf:type schema:CreativeWork
178 https://doi.org/10.1002/jmv.23248 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040806001
179 rdf:type schema:CreativeWork
180 https://doi.org/10.1002/path.1805 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026057429
181 rdf:type schema:CreativeWork
182 https://doi.org/10.1002/pmic.201000809 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037114667
183 rdf:type schema:CreativeWork
184 https://doi.org/10.1002/pmic.201200227 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030714079
185 rdf:type schema:CreativeWork
186 https://doi.org/10.1002/prot.10389 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000099680
187 rdf:type schema:CreativeWork
188 https://doi.org/10.1002/prot.10460 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031248046
189 rdf:type schema:CreativeWork
190 https://doi.org/10.1002/rmv.1868 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053337571
191 rdf:type schema:CreativeWork
192 https://doi.org/10.1006/jmbi.1993.1626 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030443070
193 rdf:type schema:CreativeWork
194 https://doi.org/10.1016/j.jprot.2012.01.016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036218556
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1016/j.neuint.2009.07.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026830599
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1016/j.virol.2006.01.022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045304049
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1016/j.virol.2008.10.025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045956259
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1016/j.virusres.2011.06.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048986569
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1021/ar000033j schema:sameAs https://app.dimensions.ai/details/publication/pub.1017409579
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1053/j.gastro.2010.01.050 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029615264
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1053/j.gastro.2010.08.043 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024976051
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1074/jbc.m410208200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030750438
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1093/nar/gkh468 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053719590
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1099/vir.0.034637-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060398857
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1099/vir.0.18883-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060399805
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1111/j.1471-4159.2008.05511.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1048819927
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1111/j.1471-4159.2010.06951.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1037576573
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1128/jvi.01399-12 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011796623
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1128/jvi.02274-16 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062713038
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1128/jvi.06072-11 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002746715
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1128/jvi.75.16.7769-7773.2001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052908790
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1146/annurev.biophys.29.1.291 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013615308
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1152/ajpgi.00374.2012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001227809
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1152/physiolgenomics.00169.2012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033219156
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1371/journal.pntd.0000437 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004390586
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1371/journal.pone.0075188 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022454895
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1371/journal.pone.0090211 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010559461
241 rdf:type schema:CreativeWork
242 https://doi.org/10.2471/blt.10.085233 schema:sameAs https://app.dimensions.ai/details/publication/pub.1070837773
243 rdf:type schema:CreativeWork
244 https://www.grid.ac/institutes/grid.250277.5 schema:alternateName National Brain Research Centre
245 schema:name National Brain Research Centre, 122052, Manesar, Haryana, India
246 rdf:type schema:Organization
247 https://www.grid.ac/institutes/grid.34980.36 schema:alternateName Indian Institute of Science Bangalore
248 schema:name Microbiology and Cell Biology, Indian Institute of Science, CV Raman Avenue, 560012, Bangalore, Karnataka, India
249 National Brain Research Centre, 122052, Manesar, Haryana, India
250 rdf:type schema:Organization
251 https://www.grid.ac/institutes/grid.418782.0 schema:alternateName Institute of Life Sciences
252 schema:name Clinical Proteomics, Institute of Life Sciences, 751023, Bhubaneswar, Odisha, India
253 rdf:type schema:Organization
254 https://www.grid.ac/institutes/grid.419478.7 schema:alternateName West Bengal State University
255 schema:name West Bengal State University, North 24 Parganas, Barasat, 700126, Kolkata, India
256 rdf:type schema:Organization
257 https://www.grid.ac/institutes/grid.59056.3f schema:alternateName University of Calcutta
258 schema:name Immunology Lab, Department of Zoology, University of Calcutta, 35, Ballygunge Circular Road, 700019, Kolkata, India
259 National Brain Research Centre, 122052, Manesar, Haryana, India
260 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...