Ontology type: schema:ScholarlyArticle Open Access: True
2018-12
AUTHORSSriparna Mukherjee, Nabonita Sengupta, Ankur Chaudhuri, Irshad Akbar, Noopur Singh, Sibani Chakraborty, Amol Ratnakar Suryawanshi, Arindam Bhattacharyya, Anirban Basu
ABSTRACTJapanese Encephalitis Virus (JEV), a globally important pathogen, belongs to the family Flaviviridae, is transmitted between vertebrate hosts by mosquitoes, principally by Culex tritaeniorhynchus. The E-glycoprotein of the virus mediates its attachment to the host cell receptors. In this study, we cloned and purified JEV E-glycoprotein in pET28a vector using E. coli BL21 (DE3) cells. A pull down assay was performed using plasma membrane fraction of BALB/c mouse brain and E-glycoprotein as a bait protein. 2-Dimensional Gel Electrophoresis based separation of the interacting proteins was analyzed by mass spectrometry. Among all the identified partners of E-glycoprotein, PLVAP (Plasmalemma vesicle associated protein) and GKN3 (Gastrokine3) showed significant up-regulation in both JEV infected mouse brain and neuro2a cells. In-silico studies also predicted significant interaction of these receptors with E-glycoprotein. Additionally, overexperssion and silencing of these receptors resulted in increase and reduction in viral load respectively, suggesting them as two critical cellular receptors governing JEV entry and propagation in neurons. In support, we observed significant expression of PLVAP but not GKN3 in post-mortem autopsied human brain tissue. Our results establish two novel receptor proteins in neurons in case of JEV infection, thus providing potential targets for antiviral research. More... »
PAGES11784
http://scigraph.springernature.com/pub.10.1038/s41598-018-30054-z
DOIhttp://dx.doi.org/10.1038/s41598-018-30054-z
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1105928938
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/30082709
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biochemistry and Cell Biology",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "University of Calcutta",
"id": "https://www.grid.ac/institutes/grid.59056.3f",
"name": [
"National Brain Research Centre, 122052, Manesar, Haryana, India",
"Immunology Lab, Department of Zoology, University of Calcutta, 35, Ballygunge Circular Road, 700019, Kolkata, India"
],
"type": "Organization"
},
"familyName": "Mukherjee",
"givenName": "Sriparna",
"id": "sg:person.0605761546.99",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0605761546.99"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Indian Institute of Science Bangalore",
"id": "https://www.grid.ac/institutes/grid.34980.36",
"name": [
"National Brain Research Centre, 122052, Manesar, Haryana, India",
"Microbiology and Cell Biology, Indian Institute of Science, CV Raman Avenue, 560012, Bangalore, Karnataka, India"
],
"type": "Organization"
},
"familyName": "Sengupta",
"givenName": "Nabonita",
"id": "sg:person.01345457340.13",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01345457340.13"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "West Bengal State University",
"id": "https://www.grid.ac/institutes/grid.419478.7",
"name": [
"West Bengal State University, North 24 Parganas, Barasat, 700126, Kolkata, India"
],
"type": "Organization"
},
"familyName": "Chaudhuri",
"givenName": "Ankur",
"id": "sg:person.01152234460.59",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01152234460.59"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "National Brain Research Centre",
"id": "https://www.grid.ac/institutes/grid.250277.5",
"name": [
"National Brain Research Centre, 122052, Manesar, Haryana, India"
],
"type": "Organization"
},
"familyName": "Akbar",
"givenName": "Irshad",
"id": "sg:person.013667404666.56",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013667404666.56"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "National Brain Research Centre",
"id": "https://www.grid.ac/institutes/grid.250277.5",
"name": [
"National Brain Research Centre, 122052, Manesar, Haryana, India"
],
"type": "Organization"
},
"familyName": "Singh",
"givenName": "Noopur",
"id": "sg:person.015712361313.54",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015712361313.54"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "West Bengal State University",
"id": "https://www.grid.ac/institutes/grid.419478.7",
"name": [
"West Bengal State University, North 24 Parganas, Barasat, 700126, Kolkata, India"
],
"type": "Organization"
},
"familyName": "Chakraborty",
"givenName": "Sibani",
"id": "sg:person.01266463060.51",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01266463060.51"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Institute of Life Sciences",
"id": "https://www.grid.ac/institutes/grid.418782.0",
"name": [
"Clinical Proteomics, Institute of Life Sciences, 751023, Bhubaneswar, Odisha, India"
],
"type": "Organization"
},
"familyName": "Suryawanshi",
"givenName": "Amol Ratnakar",
"id": "sg:person.0636736703.09",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0636736703.09"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Calcutta",
"id": "https://www.grid.ac/institutes/grid.59056.3f",
"name": [
"Immunology Lab, Department of Zoology, University of Calcutta, 35, Ballygunge Circular Road, 700019, Kolkata, India"
],
"type": "Organization"
},
"familyName": "Bhattacharyya",
"givenName": "Arindam",
"id": "sg:person.0765211606.93",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0765211606.93"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "National Brain Research Centre",
"id": "https://www.grid.ac/institutes/grid.250277.5",
"name": [
"National Brain Research Centre, 122052, Manesar, Haryana, India"
],
"type": "Organization"
},
"familyName": "Basu",
"givenName": "Anirban",
"id": "sg:person.0703222001.31",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0703222001.31"
],
"type": "Person"
}
],
"citation": [
{
"id": "https://doi.org/10.1002/prot.10389",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000099680"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1152/ajpgi.00374.2012",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001227809"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/jvi.06072-11",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1002746715"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1371/journal.pntd.0000437",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004390586"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1371/journal.pone.0090211",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010559461"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/jvi.01399-12",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1011796623"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/1471-2202-9-29",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1013443095",
"https://doi.org/10.1186/1471-2202-9-29"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1146/annurev.biophys.29.1.291",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1013615308"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/ar000033j",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1017409579"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/ar000033j",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1017409579"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1371/journal.pone.0075188",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022454895"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1053/j.gastro.2010.08.043",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1024976051"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/path.1805",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026057429"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/path.1805",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026057429"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.neuint.2009.07.006",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026830599"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1053/j.gastro.2010.01.050",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1029615264"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1006/jmbi.1993.1626",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1030443070"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/pmic.201200227",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1030714079"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1074/jbc.m410208200",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1030750438"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/prot.10460",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1031248046"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/375291a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1032366356",
"https://doi.org/10.1038/375291a0"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1152/physiolgenomics.00169.2012",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1033219156"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.jprot.2012.01.016",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036218556"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/pmic.201000809",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1037114667"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1471-4159.2010.06951.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1037576573"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1471-4159.2010.06951.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1037576573"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/jmv.23248",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1040806001"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/1743-422x-8-128",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1041755117",
"https://doi.org/10.1186/1743-422x-8-128"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/s12974-016-0486-x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1042081468",
"https://doi.org/10.1186/s12974-016-0486-x"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.virol.2006.01.022",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045304049"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.virol.2008.10.025",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045956259"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/cddis.2016.394",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1046283415",
"https://doi.org/10.1038/cddis.2016.394"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1471-4159.2008.05511.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1048819927"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.virusres.2011.06.008",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1048986569"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/1097-0142(19931115)72:10<3061::aid-cncr2820721031>3.0.co;2-6",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1051271346"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/jvi.75.16.7769-7773.2001",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1052908790"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/rmv.1868",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1053337571"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/nar/gkh468",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1053719590"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1099/vir.0.034637-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1060398857"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1099/vir.0.18883-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1060399805"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/jvi.02274-16",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1062713038"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.2471/blt.10.085233",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1070837773"
],
"type": "CreativeWork"
}
],
"datePublished": "2018-12",
"datePublishedReg": "2018-12-01",
"description": "Japanese Encephalitis Virus (JEV), a globally important pathogen, belongs to the family Flaviviridae, is transmitted between vertebrate hosts by mosquitoes, principally by Culex tritaeniorhynchus. The E-glycoprotein of the virus mediates its attachment to the host cell receptors. In this study, we cloned and purified JEV E-glycoprotein in pET28a vector using E. coli BL21 (DE3) cells. A pull down assay was performed using plasma membrane fraction of BALB/c mouse brain and E-glycoprotein as a bait protein. 2-Dimensional Gel Electrophoresis based separation of the interacting proteins was analyzed by mass spectrometry. Among all the identified partners of E-glycoprotein, PLVAP (Plasmalemma vesicle associated protein) and GKN3 (Gastrokine3) showed significant up-regulation in both JEV infected mouse brain and neuro2a cells. In-silico studies also predicted significant interaction of these receptors with E-glycoprotein. Additionally, overexperssion and silencing of these receptors resulted in increase and reduction in viral load respectively, suggesting them as two critical cellular receptors governing JEV entry and propagation in neurons. In support, we observed significant expression of PLVAP but not GKN3 in post-mortem autopsied human brain tissue. Our results establish two novel receptor proteins in neurons in case of JEV infection, thus providing potential targets for antiviral research.",
"genre": "research_article",
"id": "sg:pub.10.1038/s41598-018-30054-z",
"inLanguage": [
"en"
],
"isAccessibleForFree": true,
"isPartOf": [
{
"id": "sg:journal.1045337",
"issn": [
"2045-2322"
],
"name": "Scientific Reports",
"type": "Periodical"
},
{
"issueNumber": "1",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "8"
}
],
"name": "PLVAP and GKN3 Are Two Critical Host Cell Receptors Which Facilitate Japanese Encephalitis Virus Entry Into Neurons",
"pagination": "11784",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"b1ecc2af6f00d83f0ff58857bdcafa63d10aeec9b4f1072f9d9325a070fcbec3"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"30082709"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"101563288"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1038/s41598-018-30054-z"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1105928938"
]
}
],
"sameAs": [
"https://doi.org/10.1038/s41598-018-30054-z",
"https://app.dimensions.ai/details/publication/pub.1105928938"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-10T20:55",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8684_00000571.jsonl",
"type": "ScholarlyArticle",
"url": "https://www.nature.com/articles/s41598-018-30054-z"
}
]
Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-30054-z'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-30054-z'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-30054-z'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-30054-z'
This table displays all metadata directly associated to this object as RDF triples.
260 TRIPLES
21 PREDICATES
68 URIs
21 LITERALS
9 BLANK NODES