Viscoelastic properties of Pseudomonas aeruginosa variant biofilms View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-06-26

AUTHORS

Erin S. Gloag, Guy K. German, Paul Stoodley, Daniel J. Wozniak

ABSTRACT

Pseudomonas aeruginosa evolves during chronic pulmonary infections of cystic fibrosis (CF) patients, forming pathoadapted variants that are persistent. Mucoid and rugose small-colony variants (RSCVs) are typically isolated from sputum of CF patients. These variants overproduce exopolysaccharides in the biofilm extracellular polymeric substance (EPS). Currently, changes to the biophysical properties of RSCV and mucoid biofilms due to variations in EPS are not well understood. This knowledge may reveal how lung infections resist host clearance mechanisms. Here, we used mechanical indentation and shear rheometry to analyse the viscoelasticity of RSCV and mucoid colony-biofilms compared to their isogenic parent at 2-, 4-, and 6-d. While the viscoelasticity of parental colony-biofilms underwent fluctuating temporal changes, in contrast, RSCV and mucoid colony-biofilms showed a gradual progression to more elastic-solid behaviour. Theoretical indices of mucociliary and cough clearance predict that mature 6-d parental and RSCV biofilms may show reduced cough clearance from the lung, while early mucoid biofilms may show reduced clearance by both mechanisms. We propose that viscoelasticity be considered a virulence property of biofilms. More... »

PAGES

9691

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-018-28009-5

DOI

http://dx.doi.org/10.1038/s41598-018-28009-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1105019208

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29946126


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