The intrinsically disordered E-domains regulate the IGF-1 prohormones stability, subcellular localisation and secretion View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-12

AUTHORS

Giosuè Annibalini, Serena Contarelli, Mauro De Santi, Roberta Saltarelli, Laura Di Patria, Michele Guescini, Anna Villarini, Giorgio Brandi, Vilberto Stocchi, Elena Barbieri

ABSTRACT

Insulin-like growth factor-1 (IGF-1) is synthesised as a prohormone (proIGF-1) requiring enzymatic activity to yield the mature IGF-1. Three proIGF-1s are encoded by alternatively spliced IGF-1 mRNAs: proIGF-1Ea, proIGF-1Eb and proIGF-1Ec. These proIGF-1s have a common IGF-1 mature sequence but different E-domains. The structure of the E-domains has not been resolved, and their molecular functions are still unclear. Here, we show that E-domains are Intrinsically Disordered Regions that have distinct regulatory functions on proIGF-1s production. In particular, we identified a highly conserved N-glycosylation site in the Ea-domain, which regulated intracellular proIGF-1Ea level preventing its proteasome-mediated degradation. The inhibition of N-glycosylation by tunicamycin or glucose starvation markedly reduced proIGF-1Ea and mature IGF-1 production. Interestingly, 2-deoxyglucose, a glucose and mannose analogue, increased proIGF-1Ea and mature IGF-1 levels, probably leading to an accumulation of an under-glycosylated proIGF-1Ea that was still stable and efficiently secreted. The proIGF-1Eb and proIGF-1Ec were devoid of N-glycosylation sites, and hence their production was unaffected by N-glycosylation inhibitors. Moreover, we demonstrated that alternative Eb- and Ec-domains controlled the subcellular localisation of proIGF-1s, leading to the nuclear accumulation of both proIGF-1Eb and proIGF-1Ec. Our results demonstrated that E-domains are regulatory elements that control IGF-1 production and secretion. More... »

PAGES

8919

References to SciGraph publications

  • 2015-12. The role of the insulin-like growth factor-1 system in breast cancer in MOLECULAR CANCER
  • 2016-09. Looking at the carcinogenicity of human insulin analogues via the intrinsic disorder prism in SCIENTIFIC REPORTS
  • 2017-09. To be disordered or not to be disordered: is that still a question for proteins in the cell? in CELLULAR AND MOLECULAR LIFE SCIENCES
  • 2011-03-02. Analyzing mRNA Localization to the Endoplasmic Reticulum via Cell Fractionation in RNA DETECTION AND VISUALIZATION
  • 2012. Identifying Disordered Regions in Proteins by Limited Proteolysis in INTRINSICALLY DISORDERED PROTEIN ANALYSIS
  • 2002-04. Retro-translocation of proteins from the endoplasmic reticulum into the cytosol in NATURE REVIEWS MOLECULAR CELL BIOLOGY
  • 2016-04. Human IGF1 pro-forms induce breast cancer cell proliferation via the IGF1 receptor in CELLULAR ONCOLOGY
  • 2012-10. The nuclear localization of MGF receptor in osteoblasts under mechanical stimulation in MOLECULAR AND CELLULAR BIOCHEMISTRY
  • 2015-12. Finite mixture clustering of human tissues with different levels of IGF-1 splice variants mRNA transcripts in BMC BIOINFORMATICS
  • 2013-07. Conversion of 2-deoxyglucose-induced growth inhibition to cell death in normoxic tumor cells in CANCER CHEMOTHERAPY AND PHARMACOLOGY
  • 2007-03. RIZ1 repression is associated with insulin-like growth factor-1 signaling activation in chronic myeloid leukemia cell lines in ONCOGENE
  • 2014-01. The Complexity of the IGF1 Gene Splicing, Posttranslational Modification and Bioactivity in MOLECULAR MEDICINE
  • 2002-12. Nucleolar localization of an isoform of the IGF-I precursor in BMC CELL BIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41598-018-27233-3

    DOI

    http://dx.doi.org/10.1038/s41598-018-27233-3

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1104412667

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/29891966


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