A novel SNP in the 5’ regulatory region of organic anion transporter 1 is associated with chronic kidney disease View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-12

AUTHORS

Chiao-Yin Sun, Mai-Szu Wu, Chin-Chan Lee, Shu-Hong Chen, Kang-Chieh Lo, Yau-Hung Chen

ABSTRACT

We aimed to analyze the associations of single nucleotide polymorphisms (SNP) in the 5' regulatory region of the human organic anion transporter 1 (OAT1) gene with chronic kidney disease (CKD). A case-control study including age- and sex-matched groups of normal subjects and patients with CKD (n = 162 each) was designed. Direct sequencing of the 5' regulatory region (+88 to -1196 region) showed that patients with CKD had a higher frequency of the -475 SNP (T > T/G) than normal subjects (14/162 vs. 2/162). The luciferase activity assay results indicated that the -475G SNP had a higher promoter efficiency than the -475T SNP. Chromatin immunoprecipitation (ChIP) and LC/MS/MS analyses showed that the -475G SNP up-regulated 26 proteins and down-regulated 74 proteins. The Southwestern blot assay results revealed that the -475G SNP decreased the binding of Hepatoma-derived growth factor (HDGF), a transcription repressor, compared to the -475T SNP. Overexpression of HDGF significantly down-regulated OAT1 in renal tubular cells. Moreover, a zebrafish animal model showed that HDGF-knockdown zebrafish embryos had higher rates of kidney malformation than wild-type controls [18/78 (23.1%) vs. 1/30 (3.3%)]. In conclusion, our results suggest that an OAT1 SNP might be clinically associated with CKD. Renal tubular cells with the -475 SNP had increased OAT1 expression, which resulted in increased transportation of organic anion toxins into cells. Cellular accumulation of organic anion toxins caused cytotoxicity and resulted in CKD. More... »

PAGES

8085

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-018-26460-y

DOI

http://dx.doi.org/10.1038/s41598-018-26460-y

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1104162296

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29795395


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Chang Gung University", 
          "id": "https://www.grid.ac/institutes/grid.145695.a", 
          "name": [
            "Department of Nephrology, Keelung Chang Gung Memorial Hospital, Keelung, Taiwan", 
            "College of Medicine, Chang Gung University, Taoyuan, Taiwan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sun", 
        "givenName": "Chiao-Yin", 
        "id": "sg:person.01362560634.50", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01362560634.50"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Taipei Medical University Hospital", 
          "id": "https://www.grid.ac/institutes/grid.412897.1", 
          "name": [
            "Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan", 
            "Division of Nephrology, Department of Internal Medicine, Taipei Medical University Hospital, Taipei, Taiwan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wu", 
        "givenName": "Mai-Szu", 
        "id": "sg:person.0611075560.67", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0611075560.67"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chang Gung University", 
          "id": "https://www.grid.ac/institutes/grid.145695.a", 
          "name": [
            "Department of Nephrology, Keelung Chang Gung Memorial Hospital, Keelung, Taiwan", 
            "College of Medicine, Chang Gung University, Taoyuan, Taiwan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lee", 
        "givenName": "Chin-Chan", 
        "id": "sg:person.01251547704.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01251547704.00"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Keelung Chang Gung Memorial Hospital", 
          "id": "https://www.grid.ac/institutes/grid.454209.e", 
          "name": [
            "Department of Nephrology, Keelung Chang Gung Memorial Hospital, Keelung, Taiwan", 
            "Medical Research Center, Keelung Chang Gung Memorial Hospital, Keelung, Taiwan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chen", 
        "givenName": "Shu-Hong", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tamkang University", 
          "id": "https://www.grid.ac/institutes/grid.264580.d", 
          "name": [
            "Department of Chemistry, Tamkang University, New Taipei City, Tamsui, Taiwan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lo", 
        "givenName": "Kang-Chieh", 
        "id": "sg:person.07717776445.96", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07717776445.96"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tamkang University", 
          "id": "https://www.grid.ac/institutes/grid.264580.d", 
          "name": [
            "Department of Chemistry, Tamkang University, New Taipei City, Tamsui, Taiwan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chen", 
        "givenName": "Yau-Hung", 
        "id": "sg:person.01215567645.14", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01215567645.14"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/s10495-015-1200-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003129894", 
          "https://doi.org/10.1007/s10495-015-1200-7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.ki.5002104", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005887591", 
          "https://doi.org/10.1038/sj.ki.5002104"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10038-006-0398-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012773805", 
          "https://doi.org/10.1007/s10038-006-0398-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1053/j.jrn.2006.04.020", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013635933"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1111/j.1523-1755.2005.00612.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017520224", 
          "https://doi.org/10.1111/j.1523-1755.2005.00612.x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1523-1755.2005.00612.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017520224"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/aja.1002030302", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017782944"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1744-9987.2007.00515.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019326036"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1124/jpet.105.084301", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019759954"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/dvdy.21589", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022937134"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/ndt/gfq580", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023412763"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2215/cjn.02440314", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024267513"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2215/cjn.02440314", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024267513"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10157-005-0368-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025967831", 
          "https://doi.org/10.1007/s10157-005-0368-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10157-005-0368-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025967831", 
          "https://doi.org/10.1007/s10157-005-0368-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bdd.693", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026392242"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bdd.693", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026392242"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/labinvest.2011.48", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028270177", 
          "https://doi.org/10.1038/labinvest.2011.48"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jmb.2008.12.080", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029636207"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ki.2009.402", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029852657", 
          "https://doi.org/10.1038/ki.2009.402"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/sj.bjp.0704482", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031637228"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m111.249292", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031860822"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12032-009-9362-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032932872", 
          "https://doi.org/10.1007/s12032-009-9362-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12032-009-9362-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032932872", 
          "https://doi.org/10.1007/s12032-009-9362-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/ndt/gfq785", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033551905"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ydbio.2007.06.022", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037209447"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2215/cjn.00300113", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037617045"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2199-8-101", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044885503", 
          "https://doi.org/10.1186/1471-2199-8-101"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1253/circj.cj-14-0401", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046308286"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/ajprenal.00439.2004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049721472"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/mp100418u", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056212499"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/mp100418u", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056212499"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00008571-200309000-00007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060290071"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00008571-200309000-00007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060290071"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00008571-200309000-00007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060290071"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/01.asn.0000022017.96399.b2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060318835"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/01213011-200504000-00003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060333040"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/01213011-200504000-00003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060333040"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/01213011-200504000-00003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060333040"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074507381", 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/s41598-017-04949-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1090523180", 
          "https://doi.org/10.1038/s41598-017-04949-2"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2018-12", 
    "datePublishedReg": "2018-12-01", 
    "description": "We aimed to analyze the associations of single nucleotide polymorphisms (SNP) in the 5' regulatory region of the human organic anion transporter 1 (OAT1) gene with chronic kidney disease (CKD). A case-control study including age- and sex-matched groups of normal subjects and patients with CKD (n\u2009=\u2009162 each) was designed. Direct sequencing of the 5' regulatory region (+88 to -1196 region) showed that patients with CKD had a higher frequency of the -475 SNP (T\u2009>\u2009T/G) than normal subjects (14/162 vs. 2/162). The luciferase activity assay results indicated that the -475G SNP had a higher promoter efficiency than the -475T SNP. Chromatin immunoprecipitation (ChIP) and LC/MS/MS analyses showed that the -475G SNP up-regulated 26 proteins and down-regulated 74 proteins. The Southwestern blot assay results revealed that the -475G SNP decreased the binding of Hepatoma-derived growth factor (HDGF), a transcription repressor, compared to the -475T SNP. Overexpression of HDGF significantly down-regulated OAT1 in renal tubular cells. Moreover, a zebrafish animal model showed that HDGF-knockdown zebrafish embryos had higher rates of kidney malformation than wild-type controls [18/78 (23.1%) vs. 1/30 (3.3%)]. In conclusion, our results suggest that an OAT1 SNP might be clinically associated with CKD. Renal tubular cells with the -475 SNP had increased OAT1 expression, which resulted in increased transportation of organic anion toxins into cells. Cellular accumulation of organic anion toxins caused cytotoxicity and resulted in CKD.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/s41598-018-26460-y", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1045337", 
        "issn": [
          "2045-2322"
        ], 
        "name": "Scientific Reports", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "8"
      }
    ], 
    "name": "A novel SNP in the 5\u2019 regulatory region of organic anion transporter 1 is associated with chronic kidney disease", 
    "pagination": "8085", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "dcecf9edf3f8755debc0fb21d69a307f78efc9e0b708b8e2ce2c8b836382287d"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "29795395"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101563288"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/s41598-018-26460-y"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1104162296"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/s41598-018-26460-y", 
      "https://app.dimensions.ai/details/publication/pub.1104162296"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T23:34", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8693_00000571.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/s41598-018-26460-y"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-26460-y'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-26460-y'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-26460-y'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-26460-y'


 

This table displays all metadata directly associated to this object as RDF triples.

219 TRIPLES      21 PREDICATES      61 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/s41598-018-26460-y schema:about anzsrc-for:06
2 anzsrc-for:0604
3 schema:author N0da72bbfa0694a9bbf0a235b3fb2bca5
4 schema:citation sg:pub.10.1007/s10038-006-0398-1
5 sg:pub.10.1007/s10157-005-0368-5
6 sg:pub.10.1007/s10495-015-1200-7
7 sg:pub.10.1007/s12032-009-9362-1
8 sg:pub.10.1038/ki.2009.402
9 sg:pub.10.1038/labinvest.2011.48
10 sg:pub.10.1038/s41598-017-04949-2
11 sg:pub.10.1038/sj.ki.5002104
12 sg:pub.10.1111/j.1523-1755.2005.00612.x
13 sg:pub.10.1186/1471-2199-8-101
14 https://app.dimensions.ai/details/publication/pub.1074507381
15 https://doi.org/10.1002/aja.1002030302
16 https://doi.org/10.1002/bdd.693
17 https://doi.org/10.1002/dvdy.21589
18 https://doi.org/10.1016/j.jmb.2008.12.080
19 https://doi.org/10.1016/j.ydbio.2007.06.022
20 https://doi.org/10.1021/mp100418u
21 https://doi.org/10.1038/sj.bjp.0704482
22 https://doi.org/10.1053/j.jrn.2006.04.020
23 https://doi.org/10.1074/jbc.m111.249292
24 https://doi.org/10.1093/ndt/gfq580
25 https://doi.org/10.1093/ndt/gfq785
26 https://doi.org/10.1097/00008571-200309000-00007
27 https://doi.org/10.1097/01.asn.0000022017.96399.b2
28 https://doi.org/10.1097/01213011-200504000-00003
29 https://doi.org/10.1111/j.1523-1755.2005.00612.x
30 https://doi.org/10.1111/j.1744-9987.2007.00515.x
31 https://doi.org/10.1124/jpet.105.084301
32 https://doi.org/10.1152/ajprenal.00439.2004
33 https://doi.org/10.1253/circj.cj-14-0401
34 https://doi.org/10.2215/cjn.00300113
35 https://doi.org/10.2215/cjn.02440314
36 schema:datePublished 2018-12
37 schema:datePublishedReg 2018-12-01
38 schema:description We aimed to analyze the associations of single nucleotide polymorphisms (SNP) in the 5' regulatory region of the human organic anion transporter 1 (OAT1) gene with chronic kidney disease (CKD). A case-control study including age- and sex-matched groups of normal subjects and patients with CKD (n = 162 each) was designed. Direct sequencing of the 5' regulatory region (+88 to -1196 region) showed that patients with CKD had a higher frequency of the -475 SNP (T > T/G) than normal subjects (14/162 vs. 2/162). The luciferase activity assay results indicated that the -475G SNP had a higher promoter efficiency than the -475T SNP. Chromatin immunoprecipitation (ChIP) and LC/MS/MS analyses showed that the -475G SNP up-regulated 26 proteins and down-regulated 74 proteins. The Southwestern blot assay results revealed that the -475G SNP decreased the binding of Hepatoma-derived growth factor (HDGF), a transcription repressor, compared to the -475T SNP. Overexpression of HDGF significantly down-regulated OAT1 in renal tubular cells. Moreover, a zebrafish animal model showed that HDGF-knockdown zebrafish embryos had higher rates of kidney malformation than wild-type controls [18/78 (23.1%) vs. 1/30 (3.3%)]. In conclusion, our results suggest that an OAT1 SNP might be clinically associated with CKD. Renal tubular cells with the -475 SNP had increased OAT1 expression, which resulted in increased transportation of organic anion toxins into cells. Cellular accumulation of organic anion toxins caused cytotoxicity and resulted in CKD.
39 schema:genre research_article
40 schema:inLanguage en
41 schema:isAccessibleForFree true
42 schema:isPartOf N7a20dc13349f47a5b56589ca5dffeda3
43 Neda2f4aa0ecd4027995f055a15340bc5
44 sg:journal.1045337
45 schema:name A novel SNP in the 5’ regulatory region of organic anion transporter 1 is associated with chronic kidney disease
46 schema:pagination 8085
47 schema:productId N0958a43d11c44359b8fe987f7f8eea27
48 N106ce1812e9a4f389e6b8ccdde68cc22
49 N1414e3afacb64d0fa91a9d433da32038
50 N1b543a8097714fe3ab9c088e973d4b6d
51 N8a95f7f38fe04fc18d8ca9970a76987f
52 schema:sameAs https://app.dimensions.ai/details/publication/pub.1104162296
53 https://doi.org/10.1038/s41598-018-26460-y
54 schema:sdDatePublished 2019-04-10T23:34
55 schema:sdLicense https://scigraph.springernature.com/explorer/license/
56 schema:sdPublisher N1b04afc9e7c0410d913bb57a6bd80868
57 schema:url https://www.nature.com/articles/s41598-018-26460-y
58 sgo:license sg:explorer/license/
59 sgo:sdDataset articles
60 rdf:type schema:ScholarlyArticle
61 N0958a43d11c44359b8fe987f7f8eea27 schema:name pubmed_id
62 schema:value 29795395
63 rdf:type schema:PropertyValue
64 N0da72bbfa0694a9bbf0a235b3fb2bca5 rdf:first sg:person.01362560634.50
65 rdf:rest Nd9a7d3fce8144d068944a5954aee0dec
66 N106ce1812e9a4f389e6b8ccdde68cc22 schema:name dimensions_id
67 schema:value pub.1104162296
68 rdf:type schema:PropertyValue
69 N1414e3afacb64d0fa91a9d433da32038 schema:name doi
70 schema:value 10.1038/s41598-018-26460-y
71 rdf:type schema:PropertyValue
72 N1b04afc9e7c0410d913bb57a6bd80868 schema:name Springer Nature - SN SciGraph project
73 rdf:type schema:Organization
74 N1b543a8097714fe3ab9c088e973d4b6d schema:name readcube_id
75 schema:value dcecf9edf3f8755debc0fb21d69a307f78efc9e0b708b8e2ce2c8b836382287d
76 rdf:type schema:PropertyValue
77 N365fd1775ea94cdbb693560c4fe4febb rdf:first Nccf4783ebc6444b6a10226d65a61786e
78 rdf:rest N7ad7c307f76547d18815976b4ac11e5b
79 N3ee45ffc33b54101b56d5c25b4430e1e rdf:first sg:person.01215567645.14
80 rdf:rest rdf:nil
81 N7a20dc13349f47a5b56589ca5dffeda3 schema:issueNumber 1
82 rdf:type schema:PublicationIssue
83 N7ad7c307f76547d18815976b4ac11e5b rdf:first sg:person.07717776445.96
84 rdf:rest N3ee45ffc33b54101b56d5c25b4430e1e
85 N8a95f7f38fe04fc18d8ca9970a76987f schema:name nlm_unique_id
86 schema:value 101563288
87 rdf:type schema:PropertyValue
88 Nccf4783ebc6444b6a10226d65a61786e schema:affiliation https://www.grid.ac/institutes/grid.454209.e
89 schema:familyName Chen
90 schema:givenName Shu-Hong
91 rdf:type schema:Person
92 Nd9a7d3fce8144d068944a5954aee0dec rdf:first sg:person.0611075560.67
93 rdf:rest Ne2e1f9ab2ce04f4b9098ecfdd9dea9ea
94 Ne2e1f9ab2ce04f4b9098ecfdd9dea9ea rdf:first sg:person.01251547704.00
95 rdf:rest N365fd1775ea94cdbb693560c4fe4febb
96 Neda2f4aa0ecd4027995f055a15340bc5 schema:volumeNumber 8
97 rdf:type schema:PublicationVolume
98 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
99 schema:name Biological Sciences
100 rdf:type schema:DefinedTerm
101 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
102 schema:name Genetics
103 rdf:type schema:DefinedTerm
104 sg:journal.1045337 schema:issn 2045-2322
105 schema:name Scientific Reports
106 rdf:type schema:Periodical
107 sg:person.01215567645.14 schema:affiliation https://www.grid.ac/institutes/grid.264580.d
108 schema:familyName Chen
109 schema:givenName Yau-Hung
110 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01215567645.14
111 rdf:type schema:Person
112 sg:person.01251547704.00 schema:affiliation https://www.grid.ac/institutes/grid.145695.a
113 schema:familyName Lee
114 schema:givenName Chin-Chan
115 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01251547704.00
116 rdf:type schema:Person
117 sg:person.01362560634.50 schema:affiliation https://www.grid.ac/institutes/grid.145695.a
118 schema:familyName Sun
119 schema:givenName Chiao-Yin
120 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01362560634.50
121 rdf:type schema:Person
122 sg:person.0611075560.67 schema:affiliation https://www.grid.ac/institutes/grid.412897.1
123 schema:familyName Wu
124 schema:givenName Mai-Szu
125 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0611075560.67
126 rdf:type schema:Person
127 sg:person.07717776445.96 schema:affiliation https://www.grid.ac/institutes/grid.264580.d
128 schema:familyName Lo
129 schema:givenName Kang-Chieh
130 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07717776445.96
131 rdf:type schema:Person
132 sg:pub.10.1007/s10038-006-0398-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012773805
133 https://doi.org/10.1007/s10038-006-0398-1
134 rdf:type schema:CreativeWork
135 sg:pub.10.1007/s10157-005-0368-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025967831
136 https://doi.org/10.1007/s10157-005-0368-5
137 rdf:type schema:CreativeWork
138 sg:pub.10.1007/s10495-015-1200-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003129894
139 https://doi.org/10.1007/s10495-015-1200-7
140 rdf:type schema:CreativeWork
141 sg:pub.10.1007/s12032-009-9362-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032932872
142 https://doi.org/10.1007/s12032-009-9362-1
143 rdf:type schema:CreativeWork
144 sg:pub.10.1038/ki.2009.402 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029852657
145 https://doi.org/10.1038/ki.2009.402
146 rdf:type schema:CreativeWork
147 sg:pub.10.1038/labinvest.2011.48 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028270177
148 https://doi.org/10.1038/labinvest.2011.48
149 rdf:type schema:CreativeWork
150 sg:pub.10.1038/s41598-017-04949-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1090523180
151 https://doi.org/10.1038/s41598-017-04949-2
152 rdf:type schema:CreativeWork
153 sg:pub.10.1038/sj.ki.5002104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005887591
154 https://doi.org/10.1038/sj.ki.5002104
155 rdf:type schema:CreativeWork
156 sg:pub.10.1111/j.1523-1755.2005.00612.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1017520224
157 https://doi.org/10.1111/j.1523-1755.2005.00612.x
158 rdf:type schema:CreativeWork
159 sg:pub.10.1186/1471-2199-8-101 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044885503
160 https://doi.org/10.1186/1471-2199-8-101
161 rdf:type schema:CreativeWork
162 https://app.dimensions.ai/details/publication/pub.1074507381 schema:CreativeWork
163 https://doi.org/10.1002/aja.1002030302 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017782944
164 rdf:type schema:CreativeWork
165 https://doi.org/10.1002/bdd.693 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026392242
166 rdf:type schema:CreativeWork
167 https://doi.org/10.1002/dvdy.21589 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022937134
168 rdf:type schema:CreativeWork
169 https://doi.org/10.1016/j.jmb.2008.12.080 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029636207
170 rdf:type schema:CreativeWork
171 https://doi.org/10.1016/j.ydbio.2007.06.022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037209447
172 rdf:type schema:CreativeWork
173 https://doi.org/10.1021/mp100418u schema:sameAs https://app.dimensions.ai/details/publication/pub.1056212499
174 rdf:type schema:CreativeWork
175 https://doi.org/10.1038/sj.bjp.0704482 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031637228
176 rdf:type schema:CreativeWork
177 https://doi.org/10.1053/j.jrn.2006.04.020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013635933
178 rdf:type schema:CreativeWork
179 https://doi.org/10.1074/jbc.m111.249292 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031860822
180 rdf:type schema:CreativeWork
181 https://doi.org/10.1093/ndt/gfq580 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023412763
182 rdf:type schema:CreativeWork
183 https://doi.org/10.1093/ndt/gfq785 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033551905
184 rdf:type schema:CreativeWork
185 https://doi.org/10.1097/00008571-200309000-00007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060290071
186 rdf:type schema:CreativeWork
187 https://doi.org/10.1097/01.asn.0000022017.96399.b2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060318835
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1097/01213011-200504000-00003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060333040
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1111/j.1523-1755.2005.00612.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1017520224
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1111/j.1744-9987.2007.00515.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1019326036
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1124/jpet.105.084301 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019759954
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1152/ajprenal.00439.2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049721472
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1253/circj.cj-14-0401 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046308286
200 rdf:type schema:CreativeWork
201 https://doi.org/10.2215/cjn.00300113 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037617045
202 rdf:type schema:CreativeWork
203 https://doi.org/10.2215/cjn.02440314 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024267513
204 rdf:type schema:CreativeWork
205 https://www.grid.ac/institutes/grid.145695.a schema:alternateName Chang Gung University
206 schema:name College of Medicine, Chang Gung University, Taoyuan, Taiwan
207 Department of Nephrology, Keelung Chang Gung Memorial Hospital, Keelung, Taiwan
208 rdf:type schema:Organization
209 https://www.grid.ac/institutes/grid.264580.d schema:alternateName Tamkang University
210 schema:name Department of Chemistry, Tamkang University, New Taipei City, Tamsui, Taiwan
211 rdf:type schema:Organization
212 https://www.grid.ac/institutes/grid.412897.1 schema:alternateName Taipei Medical University Hospital
213 schema:name Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan
214 Division of Nephrology, Department of Internal Medicine, Taipei Medical University Hospital, Taipei, Taiwan
215 rdf:type schema:Organization
216 https://www.grid.ac/institutes/grid.454209.e schema:alternateName Keelung Chang Gung Memorial Hospital
217 schema:name Department of Nephrology, Keelung Chang Gung Memorial Hospital, Keelung, Taiwan
218 Medical Research Center, Keelung Chang Gung Memorial Hospital, Keelung, Taiwan
219 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...