Novel erythrocyte clumps revealed by an orphan gene Newtic1 in circulating blood and regenerating limbs of the adult newt View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-05-10

AUTHORS

Roman M. Casco-Robles, Akihiko Watanabe, Ko Eto, Kazuhito Takeshima, Shuichi Obata, Tsutomu Kinoshita, Takashi Ariizumi, Kei Nakatani, Tomoaki Nakada, Panagiotis A. Tsonis, Martin M. Casco-Robles, Keisuke Sakurai, Kensuke Yahata, Fumiaki Maruo, Fubito Toyama, Chikafumi Chiba

ABSTRACT

The newt, a group of urodele amphibians, has outstanding ability to repeatedly regenerate various body parts, even in the terrestrial life-stage. In this animal, when the limb is amputated, a cell mass named the blastema appears on the stump and eventually gives rise to a new functional limb. Erythrocytes (red blood cells) in most non-mammalian vertebrates, including the newt, preserve their nucleus throughout their life-span, although physiological roles of such nucleated erythrocytes, other than oxygen delivery, are not known. Here we report novel behavior of erythrocytes in the newt. We identified an orphan gene Newtic1, whose transcripts significantly increased in the blastema. Newtic1 was expressed in a subset of erythrocytes that formed a novel clump (EryC). EryC formed a complex with monocytes and was circulating throughout the body. When the limb was amputated, EryCs were newly generated in the stump and accumulated into a distal portion of the growing blastema. Our data suggested that the newt erythrocytes carried multiple secretory molecules including growth factors and matrix metalloproteases, and were capable of delivering these molecules into the blastema as a form of EryCs. This study provides insight into regulations and roles of nucleated erythrocytes, that are independent of oxygen delivery. More... »

PAGES

7455

Journal

TITLE

Scientific Reports

ISSUE

1

VOLUME

8

Author Affiliations

  • Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, 305-8572, Tsukuba, Ibaraki, Japan
  • Biological Division, Faculty of Science, Yamagata University, Kojirakawa 1-4-12, 990-8560, Yamagata, Japan
  • Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Kurokami 2-39-1, 860-8555, Chuo-ku, Kumamoto, Japan
  • Radioisotope Research Center, Nagoya University, Furo-cho, 464-8602, Chikusa-ku, Nagoya, Japan
  • Department of Anatomical Sciences, Faculty of Allied Health Sciences, Kitasato University, Kitasato 1-15-1, Minami-ku, 252-0373, Sagamihara, Kanagawa, Japan
  • Department of Life Science, Faculty of Science, Rikkyo University, Nishi-Ikebukuro 3-34-1, 171-8501, Toshima-ku, Tokyo, Japan
  • Department of Agri-Production Sciences, College of Agriculture, Tamagawa University, Tamagawagakuen 6-1-1, 194-8610, Machida, Tokyo, Japan
  • Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, 305-8572, Tsukuba, Ibaraki, Japan
  • Department of Comparative and Behavioral Medicine, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University, Kyonan-cho 1-7-1, 180-8602, Musashino, Tokyo, Japan
  • Department of Biology and Center for Tissue Regeneration and Engineering at Dayton, University of Dayton, 45469-2320, Dayton, Ohio, USA
  • Department of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, 305-8572, Tsukuba, Ibaraki, Japan
  • Graduate School of Engineering, Utsunomiya University, Yoto 7-1-2, Utsunomiya, 321-8585, Tochigi, Japan
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41598-018-25867-x

    DOI

    http://dx.doi.org/10.1038/s41598-018-25867-x

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1103817496

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/29748592


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