Adeno-associated virus 2/9 delivery of Cre recombinase in mouse primary afferents View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-12

AUTHORS

Khaled Abdallah, Francis Nadeau, Francis Bergeron, Sylvie Blouin, Véronique Blais, Kelly M. Bradbury, Christine L. Lavoie, Jean-Luc Parent, Louis Gendron

ABSTRACT

Genetically-modified animal models have significantly increased our understanding of the complex central nervous system circuits. Among these models, inducible transgenic mice whose specific gene expression can be modulated through a Cre recombinase/LoxP system are useful to study the role of specific peptides and proteins in a given population of cells. In the present study, we describe an efficient approach to selectively deliver a Cre-GFP to dorsal root ganglia (DRG) neurons. First, mice of different ages were injected in both hindpaws with a recombinant adeno-associated virus (rAAV2/9-CBA-Cre-GFP). Using this route of injection in mice at 5 days of age, we report that approximately 20% of all DRG neurons express GFP, 6 to 8 weeks after the infection. The level of infection was reduced by 50% when the virus was administered at 2 weeks of age. Additionally, the virus-mediated delivery of the Cre-GFP was also investigated via the intrathecal route. When injected intrathecally, the rAAV2/9-CBA-Cre-GFP virus infected a much higher proportion of DRG neurons than the intraplantar injection, with up to 51.6% of infected lumbar DRG neurons. Noteworthy, both routes of injection predominantly transduced DRG neurons over spinal and brain neurons. More... »

PAGES

7321

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-018-25626-y

DOI

http://dx.doi.org/10.1038/s41598-018-25626-y

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1103798239

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29743652


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