Chitosan and cloxacillin combination improve antibiotic efficacy against different lifestyle of coagulase-negative Staphylococcus isolates from chronic bovine mastitis View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-03-23

AUTHORS

María L. Breser, Verónica Felipe, Luciana P. Bohl, María S. Orellano, Paula Isaac, Agustín Conesa, Virginia E. Rivero, Silvia G. Correa, Ismael D. Bianco, Carina Porporatto

ABSTRACT

Bovine mastitis affects the health of dairy cows and the profitability of herds worldwide. Coagulase-negative staphylococci (CNS) are the most frequently isolated pathogens in bovine intramammary infection. Based on the wide range of antimicrobial, mucoadhesive and immunostimulant properties demonstrated by chitosan, we have evaluated therapy efficiency of chitosan incorporation to cloxacillin antibiotic as well as its effect against different bacterial lifestyles of seven CNS isolates from chronic intramammary infections. The therapeutic effects of combinations were evaluated on planktonic cultures, bacterial biofilms and intracellular growth in mammary epithelial cells. We found that biofilms and intracellular growth forms offered a strong protection against antibiotic therapy. On the other hand, we found that chitosan addition to cloxacillin efficiently reduced the antibiotic concentration necessary for bacterial killing in different lifestyle. Remarkably, the combined treatment was not only able to inhibit bacterial biofilm establishment and increase preformed biofilm eradication, but it also reduced intracellular bacterial viability while it increased IL-6 secretion by infected epithelial cells. These findings provide a new approach to prophylactic drying therapy that could help to improve conventional antimicrobial treatment against different forms of bacterial growth in an efficient, safer and greener manner reducing multiresistant bacteria generation and spread. More... »

PAGES

5081

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-018-23521-0

DOI

http://dx.doi.org/10.1038/s41598-018-23521-0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1101698180

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29572457


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25 schema:description Bovine mastitis affects the health of dairy cows and the profitability of herds worldwide. Coagulase-negative staphylococci (CNS) are the most frequently isolated pathogens in bovine intramammary infection. Based on the wide range of antimicrobial, mucoadhesive and immunostimulant properties demonstrated by chitosan, we have evaluated therapy efficiency of chitosan incorporation to cloxacillin antibiotic as well as its effect against different bacterial lifestyles of seven CNS isolates from chronic intramammary infections. The therapeutic effects of combinations were evaluated on planktonic cultures, bacterial biofilms and intracellular growth in mammary epithelial cells. We found that biofilms and intracellular growth forms offered a strong protection against antibiotic therapy. On the other hand, we found that chitosan addition to cloxacillin efficiently reduced the antibiotic concentration necessary for bacterial killing in different lifestyle. Remarkably, the combined treatment was not only able to inhibit bacterial biofilm establishment and increase preformed biofilm eradication, but it also reduced intracellular bacterial viability while it increased IL-6 secretion by infected epithelial cells. These findings provide a new approach to prophylactic drying therapy that could help to improve conventional antimicrobial treatment against different forms of bacterial growth in an efficient, safer and greener manner reducing multiresistant bacteria generation and spread.
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32 schema:keywords IL-6 secretion
33 addition
34 antibiotic concentrations
35 antibiotic efficacy
36 antibiotic therapy
37 antibiotics
38 antimicrobial treatment
39 approach
40 bacteria generation
41 bacterial biofilm establishment
42 bacterial biofilms
43 bacterial growth
44 bacterial killing
45 bacterial lifestyle
46 bacterial viability
47 biofilm eradication
48 biofilm establishment
49 biofilms
50 bovine intramammary infections
51 bovine mastitis
52 cells
53 chitosan
54 chitosan addition
55 chitosan incorporation
56 chronic bovine mastitis
57 chronic intramammary infections
58 cloxacillin
59 cloxacillin antibiotics
60 cloxacillin combination
61 coagulase-negative staphylococci
62 combination
63 concentration
64 conventional antimicrobial treatment
65 cows
66 culture
67 dairy cows
68 different bacterial lifestyles
69 different forms
70 different lifestyles
71 drying therapy
72 effect
73 efficacy
74 efficiency
75 epithelial cells
76 eradication
77 establishment
78 findings
79 form
80 generation
81 green manner
82 growth
83 growth forms
84 hand
85 health
86 herds
87 immunostimulant properties
88 incorporation
89 infected epithelial cells
90 infection
91 intracellular bacterial viability
92 intracellular growth
93 intracellular growth forms
94 intramammary infections
95 killing
96 lifestyle
97 mammary epithelial cells
98 manner
99 mastitis
100 multiresistant bacteria generation
101 new approach
102 pathogens
103 planktonic cultures
104 profitability
105 profitability of herds
106 properties
107 prophylactic drying therapy
108 protection
109 range
110 secretion
111 spread
112 staphylococci
113 strong protection
114 therapeutic effect
115 therapy
116 therapy efficiency
117 treatment
118 viability
119 wide range
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