Reduced background autofluorescence for cell imaging using nanodiamonds and lanthanide chelates View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-03-14

AUTHORS

Nicole M. Cordina, Nima Sayyadi, Lindsay M. Parker, Arun Everest-Dass, Louise J. Brown, Nicolle H. Packer

ABSTRACT

Bio-imaging is a key technique in tracking and monitoring important biological processes and fundamental biomolecular interactions, however the interference of background autofluorescence with targeted fluorophores is problematic for many bio-imaging applications. This study reports on two novel methods for reducing interference with cellular autofluorescence for bio-imaging. The first method uses fluorescent nanodiamonds (FNDs), containing nitrogen vacancy centers. FNDs emit at near-infrared wavelengths typically higher than most cellular autofluorescence; and when appropriately functionalized, can be used for background-free imaging of targeted biomolecules. The second method uses europium-chelating tags with long fluorescence lifetimes. These europium-chelating tags enhance background-free imaging due to the short fluorescent lifetimes of cellular autofluorescence. In this study, we used both methods to target E-selectin, a transmembrane glycoprotein that is activated by inflammation, to demonstrate background-free fluorescent staining in fixed endothelial cells. Our findings indicate that both FND and Europium based staining can improve fluorescent bio-imaging capabilities by reducing competition with cellular autofluorescence. 30 nm nanodiamonds coated with the E-selectin antibody was found to enable the most sensitive detective of E-selectin in inflamed cells, with a 40-fold increase in intensity detected. More... »

PAGES

4521

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-018-22702-1

DOI

http://dx.doi.org/10.1038/s41598-018-22702-1

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1101481638

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29540838


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/02", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Physical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0299", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Other Physical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biomarkers", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chelating Agents", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fluorescence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fluorescent Dyes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lanthanoid Series Elements", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microscopy, Fluorescence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Imaging", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nanodiamonds", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Binding", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Signal-To-Noise Ratio", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "ARC Centre of Excellence for Nanoscale BioPhotonics and Department of Molecular Sciences, Macquarie University, Sydney, NSW Australia", 
          "id": "http://www.grid.ac/institutes/grid.1004.5", 
          "name": [
            "ARC Centre of Excellence for Nanoscale BioPhotonics and Department of Molecular Sciences, Macquarie University, Sydney, NSW Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cordina", 
        "givenName": "Nicole M.", 
        "id": "sg:person.01300135607.83", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300135607.83"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "ARC Centre of Excellence for Nanoscale BioPhotonics and Department of Molecular Sciences, Macquarie University, Sydney, NSW Australia", 
          "id": "http://www.grid.ac/institutes/grid.1004.5", 
          "name": [
            "ARC Centre of Excellence for Nanoscale BioPhotonics and Department of Molecular Sciences, Macquarie University, Sydney, NSW Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sayyadi", 
        "givenName": "Nima", 
        "id": "sg:person.01307655317.34", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01307655317.34"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "ARC Centre of Excellence for Nanoscale BioPhotonics and Department of Molecular Sciences, Macquarie University, Sydney, NSW Australia", 
          "id": "http://www.grid.ac/institutes/grid.1004.5", 
          "name": [
            "ARC Centre of Excellence for Nanoscale BioPhotonics and Department of Molecular Sciences, Macquarie University, Sydney, NSW Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Parker", 
        "givenName": "Lindsay M.", 
        "id": "sg:person.0753006127.18", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0753006127.18"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute for Glycomics, Griffith University, Gold Coast, Queensland Australia", 
          "id": "http://www.grid.ac/institutes/grid.1022.1", 
          "name": [
            "ARC Centre of Excellence for Nanoscale BioPhotonics and Department of Molecular Sciences, Macquarie University, Sydney, NSW Australia", 
            "Institute for Glycomics, Griffith University, Gold Coast, Queensland Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Everest-Dass", 
        "givenName": "Arun", 
        "id": "sg:person.01275500346.77", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01275500346.77"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "ARC Centre of Excellence for Nanoscale BioPhotonics and Department of Molecular Sciences, Macquarie University, Sydney, NSW Australia", 
          "id": "http://www.grid.ac/institutes/grid.1004.5", 
          "name": [
            "ARC Centre of Excellence for Nanoscale BioPhotonics and Department of Molecular Sciences, Macquarie University, Sydney, NSW Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Brown", 
        "givenName": "Louise J.", 
        "id": "sg:person.01015752674.40", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01015752674.40"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute for Glycomics, Griffith University, Gold Coast, Queensland Australia", 
          "id": "http://www.grid.ac/institutes/grid.1022.1", 
          "name": [
            "ARC Centre of Excellence for Nanoscale BioPhotonics and Department of Molecular Sciences, Macquarie University, Sydney, NSW Australia", 
            "Institute for Glycomics, Griffith University, Gold Coast, Queensland Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Packer", 
        "givenName": "Nicolle H.", 
        "id": "sg:person.0657131714.23", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0657131714.23"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/s004410051311", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024338886", 
          "https://doi.org/10.1007/s004410051311"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nnano.2011.209", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006955585", 
          "https://doi.org/10.1038/nnano.2011.209"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-1-60327-198-1_6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013677522", 
          "https://doi.org/10.1007/978-1-60327-198-1_6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-1-4939-2257-4_23", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011063028", 
          "https://doi.org/10.1007/978-1-4939-2257-4_23"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nnano.2016.260", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008806736", 
          "https://doi.org/10.1038/nnano.2016.260"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1006963717646", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026365101", 
          "https://doi.org/10.1023/a:1006963717646"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nnano.2010.56", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046195235", 
          "https://doi.org/10.1038/nnano.2010.56"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12551-011-0056-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013128822", 
          "https://doi.org/10.1007/s12551-011-0056-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nnano.2008.99", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010920389", 
          "https://doi.org/10.1038/nnano.2008.99"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2018-03-14", 
    "datePublishedReg": "2018-03-14", 
    "description": "Bio-imaging is a key technique in tracking and monitoring important biological processes and fundamental biomolecular interactions, however the interference of background autofluorescence with targeted fluorophores is problematic for many bio-imaging applications. This study reports on two novel methods for reducing interference with cellular autofluorescence for bio-imaging. The first method uses fluorescent nanodiamonds (FNDs), containing nitrogen vacancy centers. FNDs emit at near-infrared wavelengths typically higher than most cellular autofluorescence; and when appropriately functionalized, can be used for background-free imaging of targeted biomolecules. The second method uses europium-chelating tags with long fluorescence lifetimes. These europium-chelating tags enhance background-free imaging due to the short fluorescent lifetimes of cellular autofluorescence. In this study, we used both methods to target E-selectin, a transmembrane glycoprotein that is activated by inflammation, to demonstrate background-free fluorescent staining in fixed endothelial cells. Our findings indicate that both FND and Europium based staining can improve fluorescent bio-imaging capabilities by reducing competition with cellular autofluorescence. 30\u2009nm nanodiamonds coated with the E-selectin antibody was found to enable the most sensitive detective of E-selectin in inflamed cells, with a 40-fold increase in intensity detected.", 
    "genre": "article", 
    "id": "sg:pub.10.1038/s41598-018-22702-1", 
    "inLanguage": "en", 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.3930688", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1045337", 
        "issn": [
          "2045-2322"
        ], 
        "name": "Scientific Reports", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "8"
      }
    ], 
    "keywords": [
      "fluorescent nanodiamonds", 
      "background-free imaging", 
      "bio-imaging applications", 
      "long fluorescence lifetime", 
      "short fluorescent lifetime", 
      "biomolecular interactions", 
      "lanthanide chelates", 
      "background autofluorescence", 
      "cellular autofluorescence", 
      "fluorescent lifetime", 
      "cell imaging", 
      "fluorescence lifetime", 
      "nanodiamonds", 
      "nitrogen-vacancy centers", 
      "important biological processes", 
      "vacancy centers", 
      "biomolecules", 
      "chelates", 
      "fluorophores", 
      "europium", 
      "key techniques", 
      "biological processes", 
      "interference", 
      "inflamed cells", 
      "lifetime", 
      "fluorescent", 
      "tags", 
      "novel method", 
      "autofluorescence", 
      "first method", 
      "second method", 
      "method", 
      "interaction", 
      "wavelength", 
      "applications", 
      "imaging", 
      "capability", 
      "intensity", 
      "process", 
      "glycoprotein", 
      "technique", 
      "cells", 
      "study", 
      "center", 
      "antibodies", 
      "increase", 
      "tracking", 
      "endothelial cells", 
      "competition", 
      "selectin antibody", 
      "transmembrane glycoprotein", 
      "staining", 
      "selectin", 
      "findings", 
      "detective", 
      "inflammation", 
      "fundamental biomolecular interactions", 
      "targeted fluorophores", 
      "most cellular autofluorescence", 
      "europium-chelating tags", 
      "background-free fluorescent", 
      "fluorescent bio-imaging capabilities", 
      "bio-imaging capabilities", 
      "sensitive detective", 
      "Reduced background autofluorescence"
    ], 
    "name": "Reduced background autofluorescence for cell imaging using nanodiamonds and lanthanide chelates", 
    "pagination": "4521", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1101481638"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/s41598-018-22702-1"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "29540838"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/s41598-018-22702-1", 
      "https://app.dimensions.ai/details/publication/pub.1101481638"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2021-11-01T18:35", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20211101/entities/gbq_results/article/article_788.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1038/s41598-018-22702-1"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-22702-1'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-22702-1'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-22702-1'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-22702-1'


 

This table displays all metadata directly associated to this object as RDF triples.

243 TRIPLES      22 PREDICATES      110 URIs      93 LITERALS      17 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/s41598-018-22702-1 schema:about N1687adcd45734a3b9c659645b62ba7e2
2 N1df96704ac86407bb1e28f9e85a6efe2
3 N2ca584f2637c480d8eb02baa230458c0
4 N2d796764ea0b47fab3d1fb68974daa44
5 N6fdfad409afb4bb4ab6fb50af4c94007
6 N75b979e1d0be4470869b77bd808a2d10
7 N7b1cb41a057a4e26ba495896d091ee6c
8 N90193dff357f426bafe0066aaa8b7ea8
9 N9f09a26df87b40ef82554552fbfcc51b
10 Nd371e21762ac4333bca986dc9cc5814c
11 anzsrc-for:02
12 anzsrc-for:0299
13 schema:author N62b99a68d3ce4a7aa72d8f8a308f66ed
14 schema:citation sg:pub.10.1007/978-1-4939-2257-4_23
15 sg:pub.10.1007/978-1-60327-198-1_6
16 sg:pub.10.1007/s004410051311
17 sg:pub.10.1007/s12551-011-0056-5
18 sg:pub.10.1023/a:1006963717646
19 sg:pub.10.1038/nnano.2008.99
20 sg:pub.10.1038/nnano.2010.56
21 sg:pub.10.1038/nnano.2011.209
22 sg:pub.10.1038/nnano.2016.260
23 schema:datePublished 2018-03-14
24 schema:datePublishedReg 2018-03-14
25 schema:description Bio-imaging is a key technique in tracking and monitoring important biological processes and fundamental biomolecular interactions, however the interference of background autofluorescence with targeted fluorophores is problematic for many bio-imaging applications. This study reports on two novel methods for reducing interference with cellular autofluorescence for bio-imaging. The first method uses fluorescent nanodiamonds (FNDs), containing nitrogen vacancy centers. FNDs emit at near-infrared wavelengths typically higher than most cellular autofluorescence; and when appropriately functionalized, can be used for background-free imaging of targeted biomolecules. The second method uses europium-chelating tags with long fluorescence lifetimes. These europium-chelating tags enhance background-free imaging due to the short fluorescent lifetimes of cellular autofluorescence. In this study, we used both methods to target E-selectin, a transmembrane glycoprotein that is activated by inflammation, to demonstrate background-free fluorescent staining in fixed endothelial cells. Our findings indicate that both FND and Europium based staining can improve fluorescent bio-imaging capabilities by reducing competition with cellular autofluorescence. 30 nm nanodiamonds coated with the E-selectin antibody was found to enable the most sensitive detective of E-selectin in inflamed cells, with a 40-fold increase in intensity detected.
26 schema:genre article
27 schema:inLanguage en
28 schema:isAccessibleForFree true
29 schema:isPartOf N6e16126ecded437190535d4a820205b5
30 Nd5519e52468e4c63909b7c53e498f3c4
31 sg:journal.1045337
32 schema:keywords Reduced background autofluorescence
33 antibodies
34 applications
35 autofluorescence
36 background autofluorescence
37 background-free fluorescent
38 background-free imaging
39 bio-imaging applications
40 bio-imaging capabilities
41 biological processes
42 biomolecular interactions
43 biomolecules
44 capability
45 cell imaging
46 cells
47 cellular autofluorescence
48 center
49 chelates
50 competition
51 detective
52 endothelial cells
53 europium
54 europium-chelating tags
55 findings
56 first method
57 fluorescence lifetime
58 fluorescent
59 fluorescent bio-imaging capabilities
60 fluorescent lifetime
61 fluorescent nanodiamonds
62 fluorophores
63 fundamental biomolecular interactions
64 glycoprotein
65 imaging
66 important biological processes
67 increase
68 inflamed cells
69 inflammation
70 intensity
71 interaction
72 interference
73 key techniques
74 lanthanide chelates
75 lifetime
76 long fluorescence lifetime
77 method
78 most cellular autofluorescence
79 nanodiamonds
80 nitrogen-vacancy centers
81 novel method
82 process
83 second method
84 selectin
85 selectin antibody
86 sensitive detective
87 short fluorescent lifetime
88 staining
89 study
90 tags
91 targeted fluorophores
92 technique
93 tracking
94 transmembrane glycoprotein
95 vacancy centers
96 wavelength
97 schema:name Reduced background autofluorescence for cell imaging using nanodiamonds and lanthanide chelates
98 schema:pagination 4521
99 schema:productId N321def9faef84c3fb20c3ac07c0a4e51
100 N6e3dabe72e4f4f118da37c64640e1257
101 N994d4c0a842242cab2ea7ed1d4c29676
102 schema:sameAs https://app.dimensions.ai/details/publication/pub.1101481638
103 https://doi.org/10.1038/s41598-018-22702-1
104 schema:sdDatePublished 2021-11-01T18:35
105 schema:sdLicense https://scigraph.springernature.com/explorer/license/
106 schema:sdPublisher Nf56f8443ced348a2895dd6a89d403e92
107 schema:url https://doi.org/10.1038/s41598-018-22702-1
108 sgo:license sg:explorer/license/
109 sgo:sdDataset articles
110 rdf:type schema:ScholarlyArticle
111 N1687adcd45734a3b9c659645b62ba7e2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Nanodiamonds
113 rdf:type schema:DefinedTerm
114 N1df96704ac86407bb1e28f9e85a6efe2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Protein Binding
116 rdf:type schema:DefinedTerm
117 N2ca584f2637c480d8eb02baa230458c0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Signal-To-Noise Ratio
119 rdf:type schema:DefinedTerm
120 N2d796764ea0b47fab3d1fb68974daa44 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Biomarkers
122 rdf:type schema:DefinedTerm
123 N321def9faef84c3fb20c3ac07c0a4e51 schema:name pubmed_id
124 schema:value 29540838
125 rdf:type schema:PropertyValue
126 N4badeb01cde347039305e21096549ad7 rdf:first sg:person.01015752674.40
127 rdf:rest Neeb2b8a118204dcabb897cedd961e2ba
128 N4de0fa0b51964a9a88a7bf6e6badb3a3 rdf:first sg:person.01307655317.34
129 rdf:rest Nb1d0203d45384e658878ff6a90a08036
130 N62b99a68d3ce4a7aa72d8f8a308f66ed rdf:first sg:person.01300135607.83
131 rdf:rest N4de0fa0b51964a9a88a7bf6e6badb3a3
132 N6e16126ecded437190535d4a820205b5 schema:volumeNumber 8
133 rdf:type schema:PublicationVolume
134 N6e3dabe72e4f4f118da37c64640e1257 schema:name doi
135 schema:value 10.1038/s41598-018-22702-1
136 rdf:type schema:PropertyValue
137 N6e5e773bdd334cf782db2006dc18bf62 rdf:first sg:person.01275500346.77
138 rdf:rest N4badeb01cde347039305e21096549ad7
139 N6fdfad409afb4bb4ab6fb50af4c94007 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Chelating Agents
141 rdf:type schema:DefinedTerm
142 N75b979e1d0be4470869b77bd808a2d10 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name Fluorescence
144 rdf:type schema:DefinedTerm
145 N7b1cb41a057a4e26ba495896d091ee6c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name Lanthanoid Series Elements
147 rdf:type schema:DefinedTerm
148 N90193dff357f426bafe0066aaa8b7ea8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
149 schema:name Fluorescent Dyes
150 rdf:type schema:DefinedTerm
151 N994d4c0a842242cab2ea7ed1d4c29676 schema:name dimensions_id
152 schema:value pub.1101481638
153 rdf:type schema:PropertyValue
154 N9f09a26df87b40ef82554552fbfcc51b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
155 schema:name Molecular Imaging
156 rdf:type schema:DefinedTerm
157 Nb1d0203d45384e658878ff6a90a08036 rdf:first sg:person.0753006127.18
158 rdf:rest N6e5e773bdd334cf782db2006dc18bf62
159 Nd371e21762ac4333bca986dc9cc5814c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
160 schema:name Microscopy, Fluorescence
161 rdf:type schema:DefinedTerm
162 Nd5519e52468e4c63909b7c53e498f3c4 schema:issueNumber 1
163 rdf:type schema:PublicationIssue
164 Neeb2b8a118204dcabb897cedd961e2ba rdf:first sg:person.0657131714.23
165 rdf:rest rdf:nil
166 Nf56f8443ced348a2895dd6a89d403e92 schema:name Springer Nature - SN SciGraph project
167 rdf:type schema:Organization
168 anzsrc-for:02 schema:inDefinedTermSet anzsrc-for:
169 schema:name Physical Sciences
170 rdf:type schema:DefinedTerm
171 anzsrc-for:0299 schema:inDefinedTermSet anzsrc-for:
172 schema:name Other Physical Sciences
173 rdf:type schema:DefinedTerm
174 sg:grant.3930688 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-018-22702-1
175 rdf:type schema:MonetaryGrant
176 sg:journal.1045337 schema:issn 2045-2322
177 schema:name Scientific Reports
178 schema:publisher Springer Nature
179 rdf:type schema:Periodical
180 sg:person.01015752674.40 schema:affiliation grid-institutes:grid.1004.5
181 schema:familyName Brown
182 schema:givenName Louise J.
183 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01015752674.40
184 rdf:type schema:Person
185 sg:person.01275500346.77 schema:affiliation grid-institutes:grid.1022.1
186 schema:familyName Everest-Dass
187 schema:givenName Arun
188 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01275500346.77
189 rdf:type schema:Person
190 sg:person.01300135607.83 schema:affiliation grid-institutes:grid.1004.5
191 schema:familyName Cordina
192 schema:givenName Nicole M.
193 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300135607.83
194 rdf:type schema:Person
195 sg:person.01307655317.34 schema:affiliation grid-institutes:grid.1004.5
196 schema:familyName Sayyadi
197 schema:givenName Nima
198 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01307655317.34
199 rdf:type schema:Person
200 sg:person.0657131714.23 schema:affiliation grid-institutes:grid.1022.1
201 schema:familyName Packer
202 schema:givenName Nicolle H.
203 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0657131714.23
204 rdf:type schema:Person
205 sg:person.0753006127.18 schema:affiliation grid-institutes:grid.1004.5
206 schema:familyName Parker
207 schema:givenName Lindsay M.
208 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0753006127.18
209 rdf:type schema:Person
210 sg:pub.10.1007/978-1-4939-2257-4_23 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011063028
211 https://doi.org/10.1007/978-1-4939-2257-4_23
212 rdf:type schema:CreativeWork
213 sg:pub.10.1007/978-1-60327-198-1_6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013677522
214 https://doi.org/10.1007/978-1-60327-198-1_6
215 rdf:type schema:CreativeWork
216 sg:pub.10.1007/s004410051311 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024338886
217 https://doi.org/10.1007/s004410051311
218 rdf:type schema:CreativeWork
219 sg:pub.10.1007/s12551-011-0056-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013128822
220 https://doi.org/10.1007/s12551-011-0056-5
221 rdf:type schema:CreativeWork
222 sg:pub.10.1023/a:1006963717646 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026365101
223 https://doi.org/10.1023/a:1006963717646
224 rdf:type schema:CreativeWork
225 sg:pub.10.1038/nnano.2008.99 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010920389
226 https://doi.org/10.1038/nnano.2008.99
227 rdf:type schema:CreativeWork
228 sg:pub.10.1038/nnano.2010.56 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046195235
229 https://doi.org/10.1038/nnano.2010.56
230 rdf:type schema:CreativeWork
231 sg:pub.10.1038/nnano.2011.209 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006955585
232 https://doi.org/10.1038/nnano.2011.209
233 rdf:type schema:CreativeWork
234 sg:pub.10.1038/nnano.2016.260 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008806736
235 https://doi.org/10.1038/nnano.2016.260
236 rdf:type schema:CreativeWork
237 grid-institutes:grid.1004.5 schema:alternateName ARC Centre of Excellence for Nanoscale BioPhotonics and Department of Molecular Sciences, Macquarie University, Sydney, NSW Australia
238 schema:name ARC Centre of Excellence for Nanoscale BioPhotonics and Department of Molecular Sciences, Macquarie University, Sydney, NSW Australia
239 rdf:type schema:Organization
240 grid-institutes:grid.1022.1 schema:alternateName Institute for Glycomics, Griffith University, Gold Coast, Queensland Australia
241 schema:name ARC Centre of Excellence for Nanoscale BioPhotonics and Department of Molecular Sciences, Macquarie University, Sydney, NSW Australia
242 Institute for Glycomics, Griffith University, Gold Coast, Queensland Australia
243 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...