Ontology type: schema:ScholarlyArticle Open Access: True
2018-12
AUTHORSJian-Min Lv, Shou-Qin Lü, Zu-Pei Liu, Juan Zhang, Bo-Xuan Gao, Zhen-Yu Yao, Yue-Xin Wu, Lawrence A. Potempa, Shang-Rong Ji, Mian Long, Yi Wu
ABSTRACTThe causal relationship between conformational folding and disulfide bonding in protein oxidative folding remains incompletely defined. Here we show a stage-dependent interplay between the two events in oxidative folding of C-reactive protein (CRP) in live cells. CRP is composed of five identical subunits, which first fold spontaneously to a near-native core with a correctly positioned C-terminal helix. This process drives the formation of the intra-subunit disulfide bond between Cys36 and Cys97. The second stage of subunit folding, however, is a non-spontaneous process with extensive restructuring driven instead by the intra-subunit disulfide bond and guided by calcium binding-mediated anchoring. With the folded subunits, pentamer assembly ensues. Our results argue that folding spontaneity is the major determinant that dictates which event acts as the driver. The stepwise folding pathway of CRP further suggests that one major route might be selected out of the many in theory for efficient folding in the cellular environment. More... »
PAGES1494
http://scigraph.springernature.com/pub.10.1038/s41598-018-20014-y
DOIhttp://dx.doi.org/10.1038/s41598-018-20014-y
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