Hydrolytic activity determination of Tail Tubular Protein A of Klebsiella pneumoniae bacteriophages towards saccharide substrates View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-12

AUTHORS

Ewa Brzozowska, Anna Pyra, Krzysztof Pawlik, Monika Janik, Sabina Górska, Natalia Urbańska, Zuzanna Drulis-Kawa, Andrzej Gamian

ABSTRACT

In this paper, the enzymatic activity, substrate specificity and antibiofilm feature of bacteriophage dual-function tail proteins are presented. So far, tail tubular proteins A-TTPAgp31 and TTPAgp44-have been considered as structural proteins of Klebsiella pneumoniae bacteriophages KP32 and KP34, respectively. Our results show that TTPAgp31 is able to hydrolyze maltose as well as Red-starch. The activity of 1 µM of the protein was calculated as 47.6 milli-Units/assay relating to the α-amylase activity. It degrades capsular polysaccharides (cPS), slime polysaccharides (sPS) and lipopolysaccharide (LPS) of K. pneumoniae PCM 2713 and shows antibiofilm reactivity towards S. aureus PCM 519 and E. faecalis PCM 2673. TTPAgp44 hydrolyses trehalose and cPS of E. faecium PCM 1859. TTPAgp44's activity was also observed in the antibiofilm test against P. aeruginosa PCM 2710 and B. subtilis PCM 2021. TTPAgp31 has been identified as α-1,4-glucosidase whereas, TTPAgp44 exhibits trehalase-like activity. Both proteins contain aspartate and glutamate residues in the β-stranded region which are essential for catalytic activity of glycoside hydrolases. The significant novelty of our results is that for the first time the bacteriophage tubular proteins are described as the unique enzymes displaying no similarity to any known phage hydrolases. They can be used as antibacterial agents directed against bacterial strains producing exopolysaccharides and forming a biofilm. More... »

PAGES

18048

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-017-18096-1

DOI

http://dx.doi.org/10.1038/s41598-017-18096-1

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1099865007

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29273737


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Ludwik Hirszfeld Institute of Immunology and Experimental Therapy", 
          "id": "https://www.grid.ac/institutes/grid.418769.5", 
          "name": [
            "Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 12 R. Weigl, 53\u2013114, Wroclaw, Poland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Brzozowska", 
        "givenName": "Ewa", 
        "id": "sg:person.01346222761.39", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01346222761.39"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Wroc\u0142aw", 
          "id": "https://www.grid.ac/institutes/grid.8505.8", 
          "name": [
            "Faculty of Chemistry, Department of Crystallography, University of Wroclaw, 14 F. Joliot-Curie, 50\u2013383, Wroclaw, Poland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pyra", 
        "givenName": "Anna", 
        "id": "sg:person.015145136257.88", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015145136257.88"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ludwik Hirszfeld Institute of Immunology and Experimental Therapy", 
          "id": "https://www.grid.ac/institutes/grid.418769.5", 
          "name": [
            "Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 12 R. Weigl, 53\u2013114, Wroclaw, Poland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pawlik", 
        "givenName": "Krzysztof", 
        "id": "sg:person.01354022723.79", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01354022723.79"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ludwik Hirszfeld Institute of Immunology and Experimental Therapy", 
          "id": "https://www.grid.ac/institutes/grid.418769.5", 
          "name": [
            "Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 12 R. Weigl, 53\u2013114, Wroclaw, Poland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Janik", 
        "givenName": "Monika", 
        "id": "sg:person.01361236253.77", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01361236253.77"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ludwik Hirszfeld Institute of Immunology and Experimental Therapy", 
          "id": "https://www.grid.ac/institutes/grid.418769.5", 
          "name": [
            "Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 12 R. Weigl, 53\u2013114, Wroclaw, Poland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "G\u00f3rska", 
        "givenName": "Sabina", 
        "id": "sg:person.0666227561.14", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0666227561.14"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Wroc\u0142aw", 
          "id": "https://www.grid.ac/institutes/grid.8505.8", 
          "name": [
            "Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 12 R. Weigl, 53\u2013114, Wroclaw, Poland", 
            "Department of Biological Science, University of Wroclaw, 35 Kuznicza, 50\u2013138, Wroclaw, Poland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Urba\u0144ska", 
        "givenName": "Natalia", 
        "id": "sg:person.014634445134.29", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014634445134.29"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Wroc\u0142aw", 
          "id": "https://www.grid.ac/institutes/grid.8505.8", 
          "name": [
            "Department of Pathogen Biology and Immunology, Institute of Genetics and Microbiology, University of Wroclaw, 63\u201377 Przybyszewskiego, 51\u2013148, Wroclaw, Poland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Drulis-Kawa", 
        "givenName": "Zuzanna", 
        "id": "sg:person.0703677125.39", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0703677125.39"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ludwik Hirszfeld Institute of Immunology and Experimental Therapy", 
          "id": "https://www.grid.ac/institutes/grid.418769.5", 
          "name": [
            "Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 12 R. Weigl, 53\u2013114, Wroclaw, Poland", 
            "Wroclaw Research Center EIT+, 147 Stablowicka, 54\u2013066, Wroclaw, Poland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gamian", 
        "givenName": "Andrzej", 
        "id": "sg:person.01231331576.36", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01231331576.36"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/j.jmb.2007.05.083", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001470489"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0042-6822(81)90162-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003084053"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/0892701021000034418", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006752307"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-2958.2003.03894.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007883873"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.186.20.6891-6901.2004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008551852"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gki408", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010393487"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/227680a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010419937", 
          "https://doi.org/10.1038/227680a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(85)90442-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011366143"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0966-842x(01)02012-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015058776"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0042-6822(56)90054-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016972978"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mmbr.00041-08", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018381537"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/1040841x.2016.1208146", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022371425"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-94-015-9702-9_15", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023062357", 
          "https://doi.org/10.1007/978-94-015-9702-9_15"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-016-7928-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024453982", 
          "https://doi.org/10.1007/s00253-016-7928-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-016-7928-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024453982", 
          "https://doi.org/10.1007/s00253-016-7928-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.vaccine.2011.11.027", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025125036"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-015-7247-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026232622", 
          "https://doi.org/10.1007/s00253-015-7247-0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nprot.2009.2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029454980", 
          "https://doi.org/10.1038/nprot.2009.2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3390/molecules14072535", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034832192"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tim.2008.11.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036771218"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3390/v7041804", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038502439"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.93.20.10584", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040371574"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1355-0306(06)71580-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042862253"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0969-2126(01)00220-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045093526"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0067950", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048416673"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.carres.2013.05.012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048846115"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2217/fmb.12.97", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049195842"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.2478/s11756-008-0169-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049266535", 
          "https://doi.org/10.2478/s11756-008-0169-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aac.45.3.649-659.2001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049592961"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1743-422x-10-100", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053687424", 
          "https://doi.org/10.1186/1743-422x-10-100"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/0022-1317-50-5-396", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060353984"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/00221287-144-11-3039", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060369695"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.5604/17322693.936090", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1073046236"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074763769", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079216948", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079752702", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079759273", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079759274", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082568716", 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-017-8224-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084020075", 
          "https://doi.org/10.1007/s00253-017-8224-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-017-8224-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084020075", 
          "https://doi.org/10.1007/s00253-017-8224-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/s41598-017-02451-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085460196", 
          "https://doi.org/10.1038/s41598-017-02451-3"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2017-12", 
    "datePublishedReg": "2017-12-01", 
    "description": "In this paper, the enzymatic activity, substrate specificity and antibiofilm feature of bacteriophage dual-function tail proteins are presented. So far, tail tubular proteins A-TTPAgp31 and TTPAgp44-have been considered as structural proteins of Klebsiella pneumoniae bacteriophages KP32 and KP34, respectively. Our results show that TTPAgp31 is able to hydrolyze maltose as well as Red-starch. The activity of 1\u2009\u00b5M of the protein was calculated as 47.6 milli-Units/assay relating to the \u03b1-amylase activity. It degrades capsular polysaccharides (cPS), slime polysaccharides (sPS) and lipopolysaccharide (LPS) of K. pneumoniae PCM 2713 and shows antibiofilm reactivity towards S. aureus PCM 519 and E. faecalis PCM 2673. TTPAgp44 hydrolyses trehalose and cPS of E. faecium PCM 1859. TTPAgp44's activity was also observed in the antibiofilm test against P. aeruginosa PCM 2710 and B. subtilis PCM 2021. TTPAgp31 has been identified as \u03b1-1,4-glucosidase whereas, TTPAgp44 exhibits trehalase-like activity. Both proteins contain aspartate and glutamate residues in the \u03b2-stranded region which are essential for catalytic activity of glycoside hydrolases. The significant novelty of our results is that for the first time the bacteriophage tubular proteins are described as the unique enzymes displaying no similarity to any known phage hydrolases. They can be used as antibacterial agents directed against bacterial strains producing exopolysaccharides and forming a biofilm.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/s41598-017-18096-1", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1045337", 
        "issn": [
          "2045-2322"
        ], 
        "name": "Scientific Reports", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "7"
      }
    ], 
    "name": "Hydrolytic activity determination of Tail Tubular Protein A of Klebsiella pneumoniae bacteriophages towards saccharide substrates", 
    "pagination": "18048", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "bd8e871a47bb1d8168dbe47e2bc46fc6585ccc920b7b03d5ff0ba7c5e8b07e39"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "29273737"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101563288"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/s41598-017-18096-1"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1099865007"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/s41598-017-18096-1", 
      "https://app.dimensions.ai/details/publication/pub.1099865007"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T20:04", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8681_00000552.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/s41598-017-18096-1"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-017-18096-1'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-017-18096-1'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-017-18096-1'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-017-18096-1'


 

This table displays all metadata directly associated to this object as RDF triples.

247 TRIPLES      21 PREDICATES      69 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/s41598-017-18096-1 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author Ne8c79457505d45cea92f983e4565c57c
4 schema:citation sg:pub.10.1007/978-94-015-9702-9_15
5 sg:pub.10.1007/s00253-015-7247-0
6 sg:pub.10.1007/s00253-016-7928-3
7 sg:pub.10.1007/s00253-017-8224-6
8 sg:pub.10.1038/227680a0
9 sg:pub.10.1038/nprot.2009.2
10 sg:pub.10.1038/s41598-017-02451-3
11 sg:pub.10.1186/1743-422x-10-100
12 sg:pub.10.2478/s11756-008-0169-x
13 https://app.dimensions.ai/details/publication/pub.1074763769
14 https://app.dimensions.ai/details/publication/pub.1079216948
15 https://app.dimensions.ai/details/publication/pub.1079752702
16 https://app.dimensions.ai/details/publication/pub.1079759273
17 https://app.dimensions.ai/details/publication/pub.1079759274
18 https://app.dimensions.ai/details/publication/pub.1082568716
19 https://doi.org/10.1016/0003-2697(85)90442-7
20 https://doi.org/10.1016/0042-6822(56)90054-x
21 https://doi.org/10.1016/0042-6822(81)90162-8
22 https://doi.org/10.1016/j.carres.2013.05.012
23 https://doi.org/10.1016/j.jmb.2007.05.083
24 https://doi.org/10.1016/j.tim.2008.11.002
25 https://doi.org/10.1016/j.vaccine.2011.11.027
26 https://doi.org/10.1016/s0966-842x(01)02012-1
27 https://doi.org/10.1016/s0969-2126(01)00220-9
28 https://doi.org/10.1016/s1355-0306(06)71580-5
29 https://doi.org/10.1046/j.1365-2958.2003.03894.x
30 https://doi.org/10.1073/pnas.93.20.10584
31 https://doi.org/10.1080/0892701021000034418
32 https://doi.org/10.1080/1040841x.2016.1208146
33 https://doi.org/10.1093/nar/gki408
34 https://doi.org/10.1099/0022-1317-50-5-396
35 https://doi.org/10.1099/00221287-144-11-3039
36 https://doi.org/10.1128/aac.45.3.649-659.2001
37 https://doi.org/10.1128/jb.186.20.6891-6901.2004
38 https://doi.org/10.1128/mmbr.00041-08
39 https://doi.org/10.1371/journal.pone.0067950
40 https://doi.org/10.2217/fmb.12.97
41 https://doi.org/10.3390/molecules14072535
42 https://doi.org/10.3390/v7041804
43 https://doi.org/10.5604/17322693.936090
44 schema:datePublished 2017-12
45 schema:datePublishedReg 2017-12-01
46 schema:description In this paper, the enzymatic activity, substrate specificity and antibiofilm feature of bacteriophage dual-function tail proteins are presented. So far, tail tubular proteins A-TTPAgp31 and TTPAgp44-have been considered as structural proteins of Klebsiella pneumoniae bacteriophages KP32 and KP34, respectively. Our results show that TTPAgp31 is able to hydrolyze maltose as well as Red-starch. The activity of 1 µM of the protein was calculated as 47.6 milli-Units/assay relating to the α-amylase activity. It degrades capsular polysaccharides (cPS), slime polysaccharides (sPS) and lipopolysaccharide (LPS) of K. pneumoniae PCM 2713 and shows antibiofilm reactivity towards S. aureus PCM 519 and E. faecalis PCM 2673. TTPAgp44 hydrolyses trehalose and cPS of E. faecium PCM 1859. TTPAgp44's activity was also observed in the antibiofilm test against P. aeruginosa PCM 2710 and B. subtilis PCM 2021. TTPAgp31 has been identified as α-1,4-glucosidase whereas, TTPAgp44 exhibits trehalase-like activity. Both proteins contain aspartate and glutamate residues in the β-stranded region which are essential for catalytic activity of glycoside hydrolases. The significant novelty of our results is that for the first time the bacteriophage tubular proteins are described as the unique enzymes displaying no similarity to any known phage hydrolases. They can be used as antibacterial agents directed against bacterial strains producing exopolysaccharides and forming a biofilm.
47 schema:genre research_article
48 schema:inLanguage en
49 schema:isAccessibleForFree true
50 schema:isPartOf N399a1b5c680e4f62b66fcc3d0997bdd3
51 N42183d86620d4d4bbc660084de958b1d
52 sg:journal.1045337
53 schema:name Hydrolytic activity determination of Tail Tubular Protein A of Klebsiella pneumoniae bacteriophages towards saccharide substrates
54 schema:pagination 18048
55 schema:productId N00ec49cf027345dba6b057083482ae16
56 Nb1024d1cdec54887a7770aea8499e861
57 Ncb7b231034e041859ad2764dc89cf8e3
58 Ne823e33db17e4d3c87980b8be8199a54
59 Nf444ef5cbb2847d78992b3279c249385
60 schema:sameAs https://app.dimensions.ai/details/publication/pub.1099865007
61 https://doi.org/10.1038/s41598-017-18096-1
62 schema:sdDatePublished 2019-04-10T20:04
63 schema:sdLicense https://scigraph.springernature.com/explorer/license/
64 schema:sdPublisher N300e54e65a19432e9af9aeafd8fd9a2d
65 schema:url https://www.nature.com/articles/s41598-017-18096-1
66 sgo:license sg:explorer/license/
67 sgo:sdDataset articles
68 rdf:type schema:ScholarlyArticle
69 N00ec49cf027345dba6b057083482ae16 schema:name readcube_id
70 schema:value bd8e871a47bb1d8168dbe47e2bc46fc6585ccc920b7b03d5ff0ba7c5e8b07e39
71 rdf:type schema:PropertyValue
72 N0c24cd45e2a7454facee3357207dc430 rdf:first sg:person.01354022723.79
73 rdf:rest N87f4f40ee0f84ef2a29e4b0b9dbc270a
74 N300e54e65a19432e9af9aeafd8fd9a2d schema:name Springer Nature - SN SciGraph project
75 rdf:type schema:Organization
76 N399a1b5c680e4f62b66fcc3d0997bdd3 schema:issueNumber 1
77 rdf:type schema:PublicationIssue
78 N42183d86620d4d4bbc660084de958b1d schema:volumeNumber 7
79 rdf:type schema:PublicationVolume
80 N556e300fade6477f89e46840334cdbf1 rdf:first sg:person.01231331576.36
81 rdf:rest rdf:nil
82 N56d5149b974a46ccaea3b49c50f085ab rdf:first sg:person.0703677125.39
83 rdf:rest N556e300fade6477f89e46840334cdbf1
84 N572535ddc85540acac630f232f37ca7b rdf:first sg:person.015145136257.88
85 rdf:rest N0c24cd45e2a7454facee3357207dc430
86 N87f4f40ee0f84ef2a29e4b0b9dbc270a rdf:first sg:person.01361236253.77
87 rdf:rest Nbd1dc0b098d2499a9a79426377e55865
88 Nb1024d1cdec54887a7770aea8499e861 schema:name dimensions_id
89 schema:value pub.1099865007
90 rdf:type schema:PropertyValue
91 Nbd1dc0b098d2499a9a79426377e55865 rdf:first sg:person.0666227561.14
92 rdf:rest Ne6c8f4dc64054b0db72b29c1d87bcd40
93 Ncb7b231034e041859ad2764dc89cf8e3 schema:name pubmed_id
94 schema:value 29273737
95 rdf:type schema:PropertyValue
96 Ne6c8f4dc64054b0db72b29c1d87bcd40 rdf:first sg:person.014634445134.29
97 rdf:rest N56d5149b974a46ccaea3b49c50f085ab
98 Ne823e33db17e4d3c87980b8be8199a54 schema:name doi
99 schema:value 10.1038/s41598-017-18096-1
100 rdf:type schema:PropertyValue
101 Ne8c79457505d45cea92f983e4565c57c rdf:first sg:person.01346222761.39
102 rdf:rest N572535ddc85540acac630f232f37ca7b
103 Nf444ef5cbb2847d78992b3279c249385 schema:name nlm_unique_id
104 schema:value 101563288
105 rdf:type schema:PropertyValue
106 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
107 schema:name Biological Sciences
108 rdf:type schema:DefinedTerm
109 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
110 schema:name Biochemistry and Cell Biology
111 rdf:type schema:DefinedTerm
112 sg:journal.1045337 schema:issn 2045-2322
113 schema:name Scientific Reports
114 rdf:type schema:Periodical
115 sg:person.01231331576.36 schema:affiliation https://www.grid.ac/institutes/grid.418769.5
116 schema:familyName Gamian
117 schema:givenName Andrzej
118 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01231331576.36
119 rdf:type schema:Person
120 sg:person.01346222761.39 schema:affiliation https://www.grid.ac/institutes/grid.418769.5
121 schema:familyName Brzozowska
122 schema:givenName Ewa
123 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01346222761.39
124 rdf:type schema:Person
125 sg:person.01354022723.79 schema:affiliation https://www.grid.ac/institutes/grid.418769.5
126 schema:familyName Pawlik
127 schema:givenName Krzysztof
128 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01354022723.79
129 rdf:type schema:Person
130 sg:person.01361236253.77 schema:affiliation https://www.grid.ac/institutes/grid.418769.5
131 schema:familyName Janik
132 schema:givenName Monika
133 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01361236253.77
134 rdf:type schema:Person
135 sg:person.014634445134.29 schema:affiliation https://www.grid.ac/institutes/grid.8505.8
136 schema:familyName Urbańska
137 schema:givenName Natalia
138 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014634445134.29
139 rdf:type schema:Person
140 sg:person.015145136257.88 schema:affiliation https://www.grid.ac/institutes/grid.8505.8
141 schema:familyName Pyra
142 schema:givenName Anna
143 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015145136257.88
144 rdf:type schema:Person
145 sg:person.0666227561.14 schema:affiliation https://www.grid.ac/institutes/grid.418769.5
146 schema:familyName Górska
147 schema:givenName Sabina
148 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0666227561.14
149 rdf:type schema:Person
150 sg:person.0703677125.39 schema:affiliation https://www.grid.ac/institutes/grid.8505.8
151 schema:familyName Drulis-Kawa
152 schema:givenName Zuzanna
153 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0703677125.39
154 rdf:type schema:Person
155 sg:pub.10.1007/978-94-015-9702-9_15 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023062357
156 https://doi.org/10.1007/978-94-015-9702-9_15
157 rdf:type schema:CreativeWork
158 sg:pub.10.1007/s00253-015-7247-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026232622
159 https://doi.org/10.1007/s00253-015-7247-0
160 rdf:type schema:CreativeWork
161 sg:pub.10.1007/s00253-016-7928-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024453982
162 https://doi.org/10.1007/s00253-016-7928-3
163 rdf:type schema:CreativeWork
164 sg:pub.10.1007/s00253-017-8224-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084020075
165 https://doi.org/10.1007/s00253-017-8224-6
166 rdf:type schema:CreativeWork
167 sg:pub.10.1038/227680a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010419937
168 https://doi.org/10.1038/227680a0
169 rdf:type schema:CreativeWork
170 sg:pub.10.1038/nprot.2009.2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029454980
171 https://doi.org/10.1038/nprot.2009.2
172 rdf:type schema:CreativeWork
173 sg:pub.10.1038/s41598-017-02451-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085460196
174 https://doi.org/10.1038/s41598-017-02451-3
175 rdf:type schema:CreativeWork
176 sg:pub.10.1186/1743-422x-10-100 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053687424
177 https://doi.org/10.1186/1743-422x-10-100
178 rdf:type schema:CreativeWork
179 sg:pub.10.2478/s11756-008-0169-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1049266535
180 https://doi.org/10.2478/s11756-008-0169-x
181 rdf:type schema:CreativeWork
182 https://app.dimensions.ai/details/publication/pub.1074763769 schema:CreativeWork
183 https://app.dimensions.ai/details/publication/pub.1079216948 schema:CreativeWork
184 https://app.dimensions.ai/details/publication/pub.1079752702 schema:CreativeWork
185 https://app.dimensions.ai/details/publication/pub.1079759273 schema:CreativeWork
186 https://app.dimensions.ai/details/publication/pub.1079759274 schema:CreativeWork
187 https://app.dimensions.ai/details/publication/pub.1082568716 schema:CreativeWork
188 https://doi.org/10.1016/0003-2697(85)90442-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011366143
189 rdf:type schema:CreativeWork
190 https://doi.org/10.1016/0042-6822(56)90054-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1016972978
191 rdf:type schema:CreativeWork
192 https://doi.org/10.1016/0042-6822(81)90162-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003084053
193 rdf:type schema:CreativeWork
194 https://doi.org/10.1016/j.carres.2013.05.012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048846115
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1016/j.jmb.2007.05.083 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001470489
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1016/j.tim.2008.11.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036771218
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1016/j.vaccine.2011.11.027 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025125036
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1016/s0966-842x(01)02012-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015058776
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1016/s0969-2126(01)00220-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045093526
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1016/s1355-0306(06)71580-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042862253
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1046/j.1365-2958.2003.03894.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1007883873
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1073/pnas.93.20.10584 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040371574
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1080/0892701021000034418 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006752307
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1080/1040841x.2016.1208146 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022371425
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1093/nar/gki408 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010393487
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1099/0022-1317-50-5-396 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060353984
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1099/00221287-144-11-3039 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060369695
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1128/aac.45.3.649-659.2001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049592961
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1128/jb.186.20.6891-6901.2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008551852
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1128/mmbr.00041-08 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018381537
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1371/journal.pone.0067950 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048416673
229 rdf:type schema:CreativeWork
230 https://doi.org/10.2217/fmb.12.97 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049195842
231 rdf:type schema:CreativeWork
232 https://doi.org/10.3390/molecules14072535 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034832192
233 rdf:type schema:CreativeWork
234 https://doi.org/10.3390/v7041804 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038502439
235 rdf:type schema:CreativeWork
236 https://doi.org/10.5604/17322693.936090 schema:sameAs https://app.dimensions.ai/details/publication/pub.1073046236
237 rdf:type schema:CreativeWork
238 https://www.grid.ac/institutes/grid.418769.5 schema:alternateName Ludwik Hirszfeld Institute of Immunology and Experimental Therapy
239 schema:name Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 12 R. Weigl, 53–114, Wroclaw, Poland
240 Wroclaw Research Center EIT+, 147 Stablowicka, 54–066, Wroclaw, Poland
241 rdf:type schema:Organization
242 https://www.grid.ac/institutes/grid.8505.8 schema:alternateName University of Wrocław
243 schema:name Department of Biological Science, University of Wroclaw, 35 Kuznicza, 50–138, Wroclaw, Poland
244 Department of Pathogen Biology and Immunology, Institute of Genetics and Microbiology, University of Wroclaw, 63–77 Przybyszewskiego, 51–148, Wroclaw, Poland
245 Faculty of Chemistry, Department of Crystallography, University of Wroclaw, 14 F. Joliot-Curie, 50–383, Wroclaw, Poland
246 Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 12 R. Weigl, 53–114, Wroclaw, Poland
247 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...