Ontology type: schema:ScholarlyArticle Open Access: True
2017-12
AUTHORSDuygu Sag, Müge Özkan, Mitchell Kronenberg, Gerhard Wingender
ABSTRACTInvariant Natural killer T (iNKT) cells rapidly produce copious amounts of multiple cytokines after in vivo activation, allowing for the direct detection of a number of cytokines directly ex vivo. However, for some cytokines this approach is suboptimal. Here, we report technical variations that allow the improved detection of IL-4, IL-10, IL-13 and IL-17A ex vivo. Furthermore, we describe an alternative approach for stimulation of iNKT cells in vitro that allows a significantly improved detection of cytokines produced by iNKT cells. Together, these protocols allow the detection of iNKT cell cytokines ex vivo and in vitro with increased sensitivity. More... »
PAGES16607
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DOIhttp://dx.doi.org/10.1038/s41598-017-16832-1
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