Profiling of different pancreatic cancer cells used as models for metastatic behaviour shows large variation in their N-glycosylation View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-12

AUTHORS

Stephanie Holst, Ana I. Belo, Elisa Giovannetti, Irma van Die, Manfred Wuhrer

ABSTRACT

To characterise pancreatic cancer cells from different sources which are used as model systems to study the metastatic behaviour in pancreatic ductal adenocarcinoma (PDAC), we compared the N-glycan imprint of four PDAC cells which were previously shown to differ in their galectin-4 expression and metastatic potential in vivo. Next to the sister cell lines Pa-Tu-8988S and Pa-Tu-8988T, which were isolated from the same liver metastasis of a PDAC, this included two primary PDAC cell cultures, PDAC1 and PDAC2. Additionally, we extended the N-glycan profiling to a normal, immortalized pancreatic duct cell line. Our results revealed major differences in the N-glycosylation of the different PDAC cells as well as compared to the control cell line, suggesting changes of the N-glycosylation in PDAC. The N-glycan profiles of the PDAC cells, however, differed vastly as well and demonstrate the diversity of PDAC model systems, which ultimately affects the interpretation of functional studies. The results from this study form the basis for further biological evaluation of the role of protein glycosylation in PDAC and highlight that conclusions from one cell line cannot be generalised, but should be regarded in the context of the corresponding phenotype. More... »

PAGES

16623

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-017-16811-6

DOI

http://dx.doi.org/10.1038/s41598-017-16811-6

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1092999963

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29192278


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