Rapid Detection of Prunus Necrotic Ringspot Virus by Reverse Transcription-cross-priming Amplification Coupled with Nucleic Acid Test Strip Cassette View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-12

AUTHORS

Ya-Yun Huo, Gui-Fen Li, Yan-Hong Qiu, Wei-Min Li, Yong-Jiang Zhang

ABSTRACT

Prunus necrotic ringspot virus (PNRSV) is one of the most devastating viruses to Prunus spp. In this study, we developed a diagnostic system RT-CPA-NATSC, wherein reverse transcription-cross-priming amplification (RT-CPA) is coupled with nucleic acid test strip cassette (NATSC), a vertical flow (VF) visualization, for PNRSV detection. The RT-CPA-NATSC assay targets the encoding gene of the PNRSV coat protein with a limit of detection of 72 copies per reaction and no cross-reaction with the known Prunus pathogenic viruses and viroids, demonstrating high sensitivity and specificity. The reaction is performed on 60 °C and can be completed less than 90 min with the prepared template RNA. Field sample test confirmed the reliability of RT-CPA-NATSC, indicating the potential application of this simple and rapid detection method in routine test of PNRSV. More... »

PAGES

16175

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-017-16536-6

DOI

http://dx.doi.org/10.1038/s41598-017-16536-6

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1092756249

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29170535


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