Character-based DNA barcoding for authentication and conservation of IUCN Red listed threatened species of genus Decalepis (Apocynaceae) View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-11-02

AUTHORS

Priyanka Mishra, Amit Kumar, Gokul Sivaraman, Ashutosh K. Shukla, Ravikumar Kaliamoorthy, Adrian Slater, Sundaresan Velusamy

ABSTRACT

The steno-endemic species of genus Decalepis are highly threatened by destructive wild harvesting. The medicinally important fleshy tuberous roots of Decalepis hamiltonii are traded as substitute, to meet the international market demand of Hemidesmus indicus. In addition, the tuberous roots of all three species of Decalepis possess similar exudates and texture, which challenges the ability of conventional techniques alone to perform accurate species authentication. This study was undertaken to generate DNA barcodes that could be utilized in monitoring and curtailing the illegal trade of these endangered species. The DNA barcode reference library was developed in BOLD database platform for candidate barcodes rbcL, matK, psbA-trnH, ITS and ITS2. The average intra-specific variations (0–0.27%) were less than the distance to nearest neighbour (0.4–11.67%) with matK and ITS. Anchoring the coding region rbcL in multigene tiered approach, the combination rbcL + matK + ITS yielded 100% species resolution, using the least number of loci combinations either with PAUP or BLOG methods to support a character-based approach. Species-specific SNP position (230 bp) in the matK region that is characteristic of D. hamiltonii could be used to design specific assays, enhancing its applicability for direct use in CITES enforcement for distinguishing it from H. indicus. More... »

PAGES

14910

References to SciGraph publications

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  • 2014-05-27. Molecular profiling of fungal assemblages in the healthy and infected roots of Decalepis arayalpathra (J. Joseph & V. Chandras) Venter, an endemic and endangered ethnomedicinal plant from Western Ghats, India in ANNALS OF MICROBIOLOGY
  • 2007-03-07. Does the DNA barcoding gap exist? – a case study in blue butterflies (Lepidoptera: Lycaenidae) in FRONTIERS IN ZOOLOGY
  • 2015-05-13. Population Dynamics and Conservation Implications of Decalepis arayalpathra (J. Joseph and V. Chandras.) Venter., a Steno Endemic Species of Western Ghats, India in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 2009-11-10. Learning to classify species with barcodes in BMC BIOINFORMATICS
  • 2015-02-09. Survey of commercial Rhodiola products revealed species diversity and potential safety issues in SCIENTIFIC REPORTS
  • 1999-03. Rapid Isolation of DNA from Dry and Fresh Samples of Plants Producing Large Amounts of Secondary Metabolites and Essential Oils in PLANT MOLECULAR BIOLOGY REPORTER
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  • 2000-01. Contributions of plant molecular systematics to studies of molecular evolution in PLANT MOLECULAR BIOLOGY
  • 2000-02. Biodiversity hotspots for conservation priorities in NATURE
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    URI

    http://scigraph.springernature.com/pub.10.1038/s41598-017-14887-8

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    http://dx.doi.org/10.1038/s41598-017-14887-8

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1092429291

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/29097709


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