Strain-dependent neutralization reveals antigenic variation of human parechovirus 3 View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-09-21

AUTHORS

Eveliina Karelehto, Sabine van der Sanden, James A. Geraets, Aušra Domanska, Lonneke van der Linden, Dionne Hoogendoorn, Gerrit Koen, Hetty van Eijk, Shabih Shakeel, Tim Beaumont, Menno de Jong, Dasja Pajkrt, Sarah J. Butcher, Katja C. Wolthers

ABSTRACT

Human parechovirus 3 (HPeV3), a member of the Picornavirus family, is frequently detected worldwide. However, the observed seropositivity rates for HPeV3 neutralizing antibodies (nAbs) vary from high in Japan to low in the Netherlands and Finland. To study if this can be explained by technical differences or antigenic diversity among HPeV3 strains included in the serological studies, we determined the neutralizing activity of Japanese and Dutch intravenous immunoglobulin batches (IVIG), a rabbit HPeV3 hyperimmune polyclonal serum, and a human HPeV3-specific monoclonal antibody (mAb) AT12-015, against the HPeV3 A308/99 prototype strain and clinical isolates from Japan, the Netherlands and Australia, collected between 1989 and 2015. The rabbit antiserum neutralized all HPeV3 isolates whereas the neutralization capacity of the IVIG batches varied, and the mAb exclusively neutralized the A308/99 strain. Mapping of the amino acid variation among a subset of the HPeV3 strains on an HPeV3 capsid structure revealed that the majority of the surface-exposed amino acid variation was located in the VP1. Furthermore, amino acid mutations in a mAb AT12-015-resistant HPeV3 A308/99 variant indicated the location for potential antigenic determinants. Virus aggregation and the observed antigenic diversity in HPeV3 can explain the varying levels of nAb seropositivity reported in previous studies. More... »

PAGES

12075

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-017-12458-5

DOI

http://dx.doi.org/10.1038/s41598-017-12458-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1091807536

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28935894


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antibodies, Neutralizing", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antigenic Variation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Capsid Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Immune Sera", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Japan", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Netherlands", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Neutralization Tests", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Parechovirus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Picornaviridae Infections", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rabbits", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Homology, Amino Acid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Species Specificity", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Medical Microbiology, Laboratory of Clinical Virology, Academic Medical Center, University of Amsterdam, 1105 AZ, Amsterdam, The Netherlands", 
          "id": "http://www.grid.ac/institutes/grid.7177.6", 
          "name": [
            "Department of Medical Microbiology, Laboratory of Clinical Virology, Academic Medical Center, University of Amsterdam, 1105 AZ, Amsterdam, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Karelehto", 
        "givenName": "Eveliina", 
        "id": "sg:person.01005044364.39", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01005044364.39"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Medical Microbiology, Laboratory of Clinical Virology, Academic Medical Center, University of Amsterdam, 1105 AZ, Amsterdam, The Netherlands", 
          "id": "http://www.grid.ac/institutes/grid.7177.6", 
          "name": [
            "Department of Medical Microbiology, Laboratory of Clinical Virology, Academic Medical Center, University of Amsterdam, 1105 AZ, Amsterdam, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "van der Sanden", 
        "givenName": "Sabine", 
        "id": "sg:person.010267367514.54", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010267367514.54"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Biotechnology & Department of Biosciences, University of Helsinki, 00014, Helsinki, Finland", 
          "id": "http://www.grid.ac/institutes/grid.7737.4", 
          "name": [
            "Institute of Biotechnology & Department of Biosciences, University of Helsinki, 00014, Helsinki, Finland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Geraets", 
        "givenName": "James A.", 
        "id": "sg:person.01340162316.43", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01340162316.43"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Biotechnology & Department of Biosciences, University of Helsinki, 00014, Helsinki, Finland", 
          "id": "http://www.grid.ac/institutes/grid.7737.4", 
          "name": [
            "Institute of Biotechnology & Department of Biosciences, University of Helsinki, 00014, Helsinki, Finland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Domanska", 
        "givenName": "Au\u0161ra", 
        "id": "sg:person.01132276400.03", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01132276400.03"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Medical Microbiology, Laboratory of Clinical Virology, Academic Medical Center, University of Amsterdam, 1105 AZ, Amsterdam, The Netherlands", 
          "id": "http://www.grid.ac/institutes/grid.7177.6", 
          "name": [
            "Department of Medical Microbiology, Laboratory of Clinical Virology, Academic Medical Center, University of Amsterdam, 1105 AZ, Amsterdam, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "van der Linden", 
        "givenName": "Lonneke", 
        "id": "sg:person.0770462260.82", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0770462260.82"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Medical Microbiology, Laboratory of Clinical Virology, Academic Medical Center, University of Amsterdam, 1105 AZ, Amsterdam, The Netherlands", 
          "id": "http://www.grid.ac/institutes/grid.7177.6", 
          "name": [
            "Department of Medical Microbiology, Laboratory of Clinical Virology, Academic Medical Center, University of Amsterdam, 1105 AZ, Amsterdam, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hoogendoorn", 
        "givenName": "Dionne", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Medical Microbiology, Laboratory of Clinical Virology, Academic Medical Center, University of Amsterdam, 1105 AZ, Amsterdam, The Netherlands", 
          "id": "http://www.grid.ac/institutes/grid.7177.6", 
          "name": [
            "Department of Medical Microbiology, Laboratory of Clinical Virology, Academic Medical Center, University of Amsterdam, 1105 AZ, Amsterdam, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Koen", 
        "givenName": "Gerrit", 
        "id": "sg:person.0722541767.97", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0722541767.97"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Medical Microbiology, Laboratory of Clinical Virology, Academic Medical Center, University of Amsterdam, 1105 AZ, Amsterdam, The Netherlands", 
          "id": "http://www.grid.ac/institutes/grid.7177.6", 
          "name": [
            "Department of Medical Microbiology, Laboratory of Clinical Virology, Academic Medical Center, University of Amsterdam, 1105 AZ, Amsterdam, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "van Eijk", 
        "givenName": "Hetty", 
        "id": "sg:person.01205246423.28", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01205246423.28"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Biotechnology & Department of Biosciences, University of Helsinki, 00014, Helsinki, Finland", 
          "id": "http://www.grid.ac/institutes/grid.7737.4", 
          "name": [
            "Institute of Biotechnology & Department of Biosciences, University of Helsinki, 00014, Helsinki, Finland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shakeel", 
        "givenName": "Shabih", 
        "id": "sg:person.01230701371.98", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01230701371.98"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "AIMM Therapeutics, Academic Medical Center, 1105 AZ, Amsterdam, The Netherlands", 
          "id": "http://www.grid.ac/institutes/grid.5650.6", 
          "name": [
            "AIMM Therapeutics, Academic Medical Center, 1105 AZ, Amsterdam, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Beaumont", 
        "givenName": "Tim", 
        "id": "sg:person.01240720571.73", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01240720571.73"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Medical Microbiology, Laboratory of Clinical Virology, Academic Medical Center, University of Amsterdam, 1105 AZ, Amsterdam, The Netherlands", 
          "id": "http://www.grid.ac/institutes/grid.7177.6", 
          "name": [
            "Department of Medical Microbiology, Laboratory of Clinical Virology, Academic Medical Center, University of Amsterdam, 1105 AZ, Amsterdam, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "de Jong", 
        "givenName": "Menno", 
        "id": "sg:person.016651406163.77", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016651406163.77"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Pediatric Infectious Diseases, Emma Children\u2019s Hospital, Academic Medical Center, 1105 AZ, Amsterdam, The Netherlands", 
          "id": "http://www.grid.ac/institutes/grid.5650.6", 
          "name": [
            "Department of Pediatric Infectious Diseases, Emma Children\u2019s Hospital, Academic Medical Center, 1105 AZ, Amsterdam, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pajkrt", 
        "givenName": "Dasja", 
        "id": "sg:person.0762472470.03", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0762472470.03"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Biotechnology & Department of Biosciences, University of Helsinki, 00014, Helsinki, Finland", 
          "id": "http://www.grid.ac/institutes/grid.7737.4", 
          "name": [
            "Institute of Biotechnology & Department of Biosciences, University of Helsinki, 00014, Helsinki, Finland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Butcher", 
        "givenName": "Sarah J.", 
        "id": "sg:person.01304461674.81", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01304461674.81"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Medical Microbiology, Laboratory of Clinical Virology, Academic Medical Center, University of Amsterdam, 1105 AZ, Amsterdam, The Netherlands", 
          "id": "http://www.grid.ac/institutes/grid.7177.6", 
          "name": [
            "Department of Medical Microbiology, Laboratory of Clinical Virology, Academic Medical Center, University of Amsterdam, 1105 AZ, Amsterdam, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wolthers", 
        "givenName": "Katja C.", 
        "id": "sg:person.01322117344.51", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01322117344.51"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1186/1471-2105-7-339", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040847479", 
          "https://doi.org/10.1186/1471-2105-7-339"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/srep44423", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084132313", 
          "https://doi.org/10.1038/srep44423"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10096-013-1906-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034700468", 
          "https://doi.org/10.1007/s10096-013-1906-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.3213", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026949821", 
          "https://doi.org/10.1038/nmeth.3213"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncomms11387", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006367616", 
          "https://doi.org/10.1038/ncomms11387"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1743-422x-10-146", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010693573", 
          "https://doi.org/10.1186/1743-422x-10-146"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2017-09-21", 
    "datePublishedReg": "2017-09-21", 
    "description": "Human parechovirus 3 (HPeV3), a member of the Picornavirus family, is frequently detected worldwide. However, the observed seropositivity rates for HPeV3 neutralizing antibodies (nAbs) vary from high in Japan to low in the Netherlands and Finland. To study if this can be explained by technical differences or antigenic diversity among HPeV3 strains included in the serological studies, we determined the neutralizing activity of Japanese and Dutch intravenous immunoglobulin batches (IVIG), a rabbit HPeV3 hyperimmune polyclonal serum, and a human HPeV3-specific monoclonal antibody (mAb) AT12-015, against the HPeV3 A308/99 prototype strain and clinical isolates from Japan, the Netherlands and Australia, collected between 1989 and 2015. The rabbit antiserum neutralized all HPeV3 isolates whereas the neutralization capacity of the IVIG batches varied, and the mAb exclusively neutralized the A308/99 strain. Mapping of the amino acid variation among a subset of the HPeV3 strains on an HPeV3 capsid structure revealed that the majority of the surface-exposed amino acid variation was located in the VP1. Furthermore, amino acid mutations in a mAb AT12-015-resistant HPeV3 A308/99 variant indicated the location for potential antigenic determinants. Virus aggregation and the observed antigenic diversity in HPeV3 can explain the varying levels of nAb seropositivity reported in previous studies.", 
    "genre": "article", 
    "id": "sg:pub.10.1038/s41598-017-12458-5", 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.4251502", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.3796473", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1045337", 
        "issn": [
          "2045-2322"
        ], 
        "name": "Scientific Reports", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "7"
      }
    ], 
    "keywords": [
      "human parechovirus 3", 
      "antigenic diversity", 
      "amino acid variations", 
      "seropositivity rate", 
      "IVIG batches", 
      "immunoglobulin batches", 
      "serological studies", 
      "clinical isolates", 
      "HPeV3", 
      "potential antigenic determinants", 
      "antigenic determinants", 
      "acid variations", 
      "prototype strain", 
      "picornavirus family", 
      "polyclonal sera", 
      "rabbit antiserum", 
      "amino acid mutations", 
      "antigenic variation", 
      "acid mutations", 
      "neutralization capacity", 
      "previous studies", 
      "seropositivity", 
      "serum", 
      "antibodies", 
      "mAbs", 
      "virus aggregation", 
      "technical differences", 
      "study", 
      "antiserum", 
      "strains", 
      "Netherlands", 
      "isolates", 
      "VP1", 
      "majority", 
      "mutations", 
      "neutralization", 
      "subset", 
      "determinants", 
      "differences", 
      "levels", 
      "activity", 
      "variants", 
      "rate", 
      "Japan", 
      "family", 
      "capsid structure", 
      "Finland", 
      "members", 
      "capacity", 
      "Australia", 
      "variation", 
      "aggregation", 
      "location", 
      "batch", 
      "diversity", 
      "mapping", 
      "structure"
    ], 
    "name": "Strain-dependent neutralization reveals antigenic variation of human parechovirus 3", 
    "pagination": "12075", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1091807536"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/s41598-017-12458-5"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "28935894"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/s41598-017-12458-5", 
      "https://app.dimensions.ai/details/publication/pub.1091807536"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-09-02T16:00", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220902/entities/gbq_results/article/article_724.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1038/s41598-017-12458-5"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-017-12458-5'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-017-12458-5'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-017-12458-5'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-017-12458-5'


 

This table displays all metadata directly associated to this object as RDF triples.

307 TRIPLES      21 PREDICATES      104 URIs      90 LITERALS      23 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/s41598-017-12458-5 schema:about N10e7a41d3d8c4661a93072d5038e4c8d
2 N167d9a20781e4b78b2c11bea497ad4a4
3 N2afd7b435e674bcd808648e0e9bd7c05
4 N2f345ca9bfc3408b8bd279eafa946fb1
5 N334eeb6a7c3448488fbb83ce0770d644
6 N3673a539588e464e8cd50e63d29cbc52
7 N4d7626888dfc4c3f889714910267d6fa
8 N5248681516af407a8b2191635c0d24bf
9 N59cae9d568fc4bfe958b80c99f1038a1
10 N64a0ebf23b134fec82a1c1541a2ab0b4
11 N698b4efb0de44de6ba60525d2631ff7b
12 Naf6b2535c0724f0fbf8dcea7d46b6274
13 Nc705a8a78dcf4900bc5667cf382c9c5e
14 Nd52295e8a9394fc6ab6d2e2cc137e5f2
15 Nde0ca5d4ee6f43908209b1673cfc9123
16 Ne76d4b18d20740b0bdfb7289de0a9bb9
17 anzsrc-for:11
18 anzsrc-for:1108
19 schema:author N317ef2e4b516490ba0488dc00afb2ff2
20 schema:citation sg:pub.10.1007/s10096-013-1906-9
21 sg:pub.10.1038/ncomms11387
22 sg:pub.10.1038/nmeth.3213
23 sg:pub.10.1038/srep44423
24 sg:pub.10.1186/1471-2105-7-339
25 sg:pub.10.1186/1743-422x-10-146
26 schema:datePublished 2017-09-21
27 schema:datePublishedReg 2017-09-21
28 schema:description Human parechovirus 3 (HPeV3), a member of the Picornavirus family, is frequently detected worldwide. However, the observed seropositivity rates for HPeV3 neutralizing antibodies (nAbs) vary from high in Japan to low in the Netherlands and Finland. To study if this can be explained by technical differences or antigenic diversity among HPeV3 strains included in the serological studies, we determined the neutralizing activity of Japanese and Dutch intravenous immunoglobulin batches (IVIG), a rabbit HPeV3 hyperimmune polyclonal serum, and a human HPeV3-specific monoclonal antibody (mAb) AT12-015, against the HPeV3 A308/99 prototype strain and clinical isolates from Japan, the Netherlands and Australia, collected between 1989 and 2015. The rabbit antiserum neutralized all HPeV3 isolates whereas the neutralization capacity of the IVIG batches varied, and the mAb exclusively neutralized the A308/99 strain. Mapping of the amino acid variation among a subset of the HPeV3 strains on an HPeV3 capsid structure revealed that the majority of the surface-exposed amino acid variation was located in the VP1. Furthermore, amino acid mutations in a mAb AT12-015-resistant HPeV3 A308/99 variant indicated the location for potential antigenic determinants. Virus aggregation and the observed antigenic diversity in HPeV3 can explain the varying levels of nAb seropositivity reported in previous studies.
29 schema:genre article
30 schema:isAccessibleForFree true
31 schema:isPartOf N07766498048d4f9d88a3437a64508780
32 Na9ecebc3434549539ba0faeb5dc12cb4
33 sg:journal.1045337
34 schema:keywords Australia
35 Finland
36 HPeV3
37 IVIG batches
38 Japan
39 Netherlands
40 VP1
41 acid mutations
42 acid variations
43 activity
44 aggregation
45 amino acid mutations
46 amino acid variations
47 antibodies
48 antigenic determinants
49 antigenic diversity
50 antigenic variation
51 antiserum
52 batch
53 capacity
54 capsid structure
55 clinical isolates
56 determinants
57 differences
58 diversity
59 family
60 human parechovirus 3
61 immunoglobulin batches
62 isolates
63 levels
64 location
65 mAbs
66 majority
67 mapping
68 members
69 mutations
70 neutralization
71 neutralization capacity
72 picornavirus family
73 polyclonal sera
74 potential antigenic determinants
75 previous studies
76 prototype strain
77 rabbit antiserum
78 rate
79 serological studies
80 seropositivity
81 seropositivity rate
82 serum
83 strains
84 structure
85 study
86 subset
87 technical differences
88 variants
89 variation
90 virus aggregation
91 schema:name Strain-dependent neutralization reveals antigenic variation of human parechovirus 3
92 schema:pagination 12075
93 schema:productId N8050299c46ff4349bfd3c62f27499620
94 Ndea118b1947948f38fc7cbf764e7be59
95 Nf08cf6a6143343338a69b78296d7d9e9
96 schema:sameAs https://app.dimensions.ai/details/publication/pub.1091807536
97 https://doi.org/10.1038/s41598-017-12458-5
98 schema:sdDatePublished 2022-09-02T16:00
99 schema:sdLicense https://scigraph.springernature.com/explorer/license/
100 schema:sdPublisher Nfc37748017dd47b0bf1ad150d73f82b7
101 schema:url https://doi.org/10.1038/s41598-017-12458-5
102 sgo:license sg:explorer/license/
103 sgo:sdDataset articles
104 rdf:type schema:ScholarlyArticle
105 N01c0a869c21a495a99858de17f06339c rdf:first sg:person.01132276400.03
106 rdf:rest Ndbba9b49c92a4bb09a05686756b65523
107 N07766498048d4f9d88a3437a64508780 schema:volumeNumber 7
108 rdf:type schema:PublicationVolume
109 N10e7a41d3d8c4661a93072d5038e4c8d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
110 schema:name Picornaviridae Infections
111 rdf:type schema:DefinedTerm
112 N167d9a20781e4b78b2c11bea497ad4a4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Immune Sera
114 rdf:type schema:DefinedTerm
115 N2afd7b435e674bcd808648e0e9bd7c05 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Humans
117 rdf:type schema:DefinedTerm
118 N2f345ca9bfc3408b8bd279eafa946fb1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name Neutralization Tests
120 rdf:type schema:DefinedTerm
121 N317ef2e4b516490ba0488dc00afb2ff2 rdf:first sg:person.01005044364.39
122 rdf:rest N31917694c12248deb2ca1bdb4035413f
123 N31917694c12248deb2ca1bdb4035413f rdf:first sg:person.010267367514.54
124 rdf:rest N844964695d80402cbcb10e31303b2eec
125 N334eeb6a7c3448488fbb83ce0770d644 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
126 schema:name Antigenic Variation
127 rdf:type schema:DefinedTerm
128 N3673a539588e464e8cd50e63d29cbc52 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name Japan
130 rdf:type schema:DefinedTerm
131 N373c9a47e8554cc99e7cbfe6a6f58a95 rdf:first sg:person.01205246423.28
132 rdf:rest N43a9d573073a4902804af105e39ef772
133 N43a9d573073a4902804af105e39ef772 rdf:first sg:person.01230701371.98
134 rdf:rest N56218036903a4c27951e1f622b942dd9
135 N4d7626888dfc4c3f889714910267d6fa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
136 schema:name Mutation
137 rdf:type schema:DefinedTerm
138 N5248681516af407a8b2191635c0d24bf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
139 schema:name Amino Acid Sequence
140 rdf:type schema:DefinedTerm
141 N56218036903a4c27951e1f622b942dd9 rdf:first sg:person.01240720571.73
142 rdf:rest N6b566f1f08854bb49d04f968b4c47ed8
143 N59cae9d568fc4bfe958b80c99f1038a1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Netherlands
145 rdf:type schema:DefinedTerm
146 N64a0ebf23b134fec82a1c1541a2ab0b4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Capsid Proteins
148 rdf:type schema:DefinedTerm
149 N698b4efb0de44de6ba60525d2631ff7b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
150 schema:name Animals
151 rdf:type schema:DefinedTerm
152 N6b566f1f08854bb49d04f968b4c47ed8 rdf:first sg:person.016651406163.77
153 rdf:rest Nd8f1cd170a7d47b88e5479bb0bf14e91
154 N8050299c46ff4349bfd3c62f27499620 schema:name pubmed_id
155 schema:value 28935894
156 rdf:type schema:PropertyValue
157 N844964695d80402cbcb10e31303b2eec rdf:first sg:person.01340162316.43
158 rdf:rest N01c0a869c21a495a99858de17f06339c
159 N97d442690eff41629313b29039beb28b schema:affiliation grid-institutes:grid.7177.6
160 schema:familyName Hoogendoorn
161 schema:givenName Dionne
162 rdf:type schema:Person
163 Na9ecebc3434549539ba0faeb5dc12cb4 schema:issueNumber 1
164 rdf:type schema:PublicationIssue
165 Naf6b2535c0724f0fbf8dcea7d46b6274 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
166 schema:name Rabbits
167 rdf:type schema:DefinedTerm
168 Nc705a8a78dcf4900bc5667cf382c9c5e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
169 schema:name Species Specificity
170 rdf:type schema:DefinedTerm
171 Ncd218979949640fd9dc28898a5ddce29 rdf:first sg:person.0722541767.97
172 rdf:rest N373c9a47e8554cc99e7cbfe6a6f58a95
173 Nd52295e8a9394fc6ab6d2e2cc137e5f2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
174 schema:name Parechovirus
175 rdf:type schema:DefinedTerm
176 Nd8f1cd170a7d47b88e5479bb0bf14e91 rdf:first sg:person.0762472470.03
177 rdf:rest Nf6603e488bde4b34a3ccbb1983990fe8
178 Ndbba9b49c92a4bb09a05686756b65523 rdf:first sg:person.0770462260.82
179 rdf:rest Neb9ef3dbbd0f4589adba3febf5207ef9
180 Nde0ca5d4ee6f43908209b1673cfc9123 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
181 schema:name Antibodies, Neutralizing
182 rdf:type schema:DefinedTerm
183 Nde0e173f987d438d9821cff69b7c860f rdf:first sg:person.01322117344.51
184 rdf:rest rdf:nil
185 Ndea118b1947948f38fc7cbf764e7be59 schema:name dimensions_id
186 schema:value pub.1091807536
187 rdf:type schema:PropertyValue
188 Ne76d4b18d20740b0bdfb7289de0a9bb9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
189 schema:name Sequence Homology, Amino Acid
190 rdf:type schema:DefinedTerm
191 Neb9ef3dbbd0f4589adba3febf5207ef9 rdf:first N97d442690eff41629313b29039beb28b
192 rdf:rest Ncd218979949640fd9dc28898a5ddce29
193 Nf08cf6a6143343338a69b78296d7d9e9 schema:name doi
194 schema:value 10.1038/s41598-017-12458-5
195 rdf:type schema:PropertyValue
196 Nf6603e488bde4b34a3ccbb1983990fe8 rdf:first sg:person.01304461674.81
197 rdf:rest Nde0e173f987d438d9821cff69b7c860f
198 Nfc37748017dd47b0bf1ad150d73f82b7 schema:name Springer Nature - SN SciGraph project
199 rdf:type schema:Organization
200 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
201 schema:name Medical and Health Sciences
202 rdf:type schema:DefinedTerm
203 anzsrc-for:1108 schema:inDefinedTermSet anzsrc-for:
204 schema:name Medical Microbiology
205 rdf:type schema:DefinedTerm
206 sg:grant.3796473 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-017-12458-5
207 rdf:type schema:MonetaryGrant
208 sg:grant.4251502 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-017-12458-5
209 rdf:type schema:MonetaryGrant
210 sg:journal.1045337 schema:issn 2045-2322
211 schema:name Scientific Reports
212 schema:publisher Springer Nature
213 rdf:type schema:Periodical
214 sg:person.01005044364.39 schema:affiliation grid-institutes:grid.7177.6
215 schema:familyName Karelehto
216 schema:givenName Eveliina
217 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01005044364.39
218 rdf:type schema:Person
219 sg:person.010267367514.54 schema:affiliation grid-institutes:grid.7177.6
220 schema:familyName van der Sanden
221 schema:givenName Sabine
222 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010267367514.54
223 rdf:type schema:Person
224 sg:person.01132276400.03 schema:affiliation grid-institutes:grid.7737.4
225 schema:familyName Domanska
226 schema:givenName Aušra
227 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01132276400.03
228 rdf:type schema:Person
229 sg:person.01205246423.28 schema:affiliation grid-institutes:grid.7177.6
230 schema:familyName van Eijk
231 schema:givenName Hetty
232 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01205246423.28
233 rdf:type schema:Person
234 sg:person.01230701371.98 schema:affiliation grid-institutes:grid.7737.4
235 schema:familyName Shakeel
236 schema:givenName Shabih
237 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01230701371.98
238 rdf:type schema:Person
239 sg:person.01240720571.73 schema:affiliation grid-institutes:grid.5650.6
240 schema:familyName Beaumont
241 schema:givenName Tim
242 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01240720571.73
243 rdf:type schema:Person
244 sg:person.01304461674.81 schema:affiliation grid-institutes:grid.7737.4
245 schema:familyName Butcher
246 schema:givenName Sarah J.
247 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01304461674.81
248 rdf:type schema:Person
249 sg:person.01322117344.51 schema:affiliation grid-institutes:grid.7177.6
250 schema:familyName Wolthers
251 schema:givenName Katja C.
252 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01322117344.51
253 rdf:type schema:Person
254 sg:person.01340162316.43 schema:affiliation grid-institutes:grid.7737.4
255 schema:familyName Geraets
256 schema:givenName James A.
257 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01340162316.43
258 rdf:type schema:Person
259 sg:person.016651406163.77 schema:affiliation grid-institutes:grid.7177.6
260 schema:familyName de Jong
261 schema:givenName Menno
262 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016651406163.77
263 rdf:type schema:Person
264 sg:person.0722541767.97 schema:affiliation grid-institutes:grid.7177.6
265 schema:familyName Koen
266 schema:givenName Gerrit
267 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0722541767.97
268 rdf:type schema:Person
269 sg:person.0762472470.03 schema:affiliation grid-institutes:grid.5650.6
270 schema:familyName Pajkrt
271 schema:givenName Dasja
272 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0762472470.03
273 rdf:type schema:Person
274 sg:person.0770462260.82 schema:affiliation grid-institutes:grid.7177.6
275 schema:familyName van der Linden
276 schema:givenName Lonneke
277 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0770462260.82
278 rdf:type schema:Person
279 sg:pub.10.1007/s10096-013-1906-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034700468
280 https://doi.org/10.1007/s10096-013-1906-9
281 rdf:type schema:CreativeWork
282 sg:pub.10.1038/ncomms11387 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006367616
283 https://doi.org/10.1038/ncomms11387
284 rdf:type schema:CreativeWork
285 sg:pub.10.1038/nmeth.3213 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026949821
286 https://doi.org/10.1038/nmeth.3213
287 rdf:type schema:CreativeWork
288 sg:pub.10.1038/srep44423 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084132313
289 https://doi.org/10.1038/srep44423
290 rdf:type schema:CreativeWork
291 sg:pub.10.1186/1471-2105-7-339 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040847479
292 https://doi.org/10.1186/1471-2105-7-339
293 rdf:type schema:CreativeWork
294 sg:pub.10.1186/1743-422x-10-146 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010693573
295 https://doi.org/10.1186/1743-422x-10-146
296 rdf:type schema:CreativeWork
297 grid-institutes:grid.5650.6 schema:alternateName AIMM Therapeutics, Academic Medical Center, 1105 AZ, Amsterdam, The Netherlands
298 Department of Pediatric Infectious Diseases, Emma Children’s Hospital, Academic Medical Center, 1105 AZ, Amsterdam, The Netherlands
299 schema:name AIMM Therapeutics, Academic Medical Center, 1105 AZ, Amsterdam, The Netherlands
300 Department of Pediatric Infectious Diseases, Emma Children’s Hospital, Academic Medical Center, 1105 AZ, Amsterdam, The Netherlands
301 rdf:type schema:Organization
302 grid-institutes:grid.7177.6 schema:alternateName Department of Medical Microbiology, Laboratory of Clinical Virology, Academic Medical Center, University of Amsterdam, 1105 AZ, Amsterdam, The Netherlands
303 schema:name Department of Medical Microbiology, Laboratory of Clinical Virology, Academic Medical Center, University of Amsterdam, 1105 AZ, Amsterdam, The Netherlands
304 rdf:type schema:Organization
305 grid-institutes:grid.7737.4 schema:alternateName Institute of Biotechnology & Department of Biosciences, University of Helsinki, 00014, Helsinki, Finland
306 schema:name Institute of Biotechnology & Department of Biosciences, University of Helsinki, 00014, Helsinki, Finland
307 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...