ICM conversion to epiblast by FGF/ERK inhibition is limited in time and requires transcription and protein degradation View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-12

AUTHORS

Sylvain Bessonnard, Sabrina Coqueran, Sandrine Vandormael-Pournin, Alexandre Dufour, Jérôme Artus, Michel Cohen-Tannoudji

ABSTRACT

Inner cell Mass (ICM) specification into epiblast (Epi) and primitive endoderm (PrE) is an asynchronous and progressive process taking place between E3.0 to E3.75 under the control of the Fibroblast Growth Factor (FGF)/Extracellular signal-Regulated Kinase (ERK) signaling pathway. Here, we have analyzed in details the kinetics of specification and found that ICM cell responsiveness to the up and down regulation of FGF signaling activity are temporally distinct. We also showed that PrE progenitors are generated later than Epi progenitors. We further demonstrated that, during this late phase of specification, a 4 hours period of FGF/ERK inhibition prior E3.75 is sufficient to convert ICM cells into Epi. Finally, we showed that ICM conversion into Epi in response to inhibition during this short time window requires both transcription and proteasome degradation. Collectively, our data give new insights into the timing and mechanisms involved in the process of ICM specification. More... »

PAGES

12285

References to SciGraph publications

Journal

TITLE

Scientific Reports

ISSUE

1

VOLUME

7

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-017-12120-0

DOI

http://dx.doi.org/10.1038/s41598-017-12120-0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1091866052

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28947813


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