Functional modulation of LHCSR1 protein from Physcomitrella patens by zeaxanthin binding and low pH View Full Text


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Article Info

DATE

2017-09-11

AUTHORS

Alberta Pinnola, Matteo Ballottari, Ilaria Bargigia, Marcelo Alcocer, Cosimo D’Andrea, Giulio Cerullo, Roberto Bassi

ABSTRACT

Light harvesting for oxygenic photosynthesis is regulated to prevent the formation of harmful photoproducts by activation of photoprotective mechanisms safely dissipating the energy absorbed in excess. Lumen acidification is the trigger for the formation of quenching states in pigment binding complexes. With the aim to uncover the photoprotective functional states responsible for excess energy dissipation in green algae and mosses, we compared the fluorescence dynamic properties of the light-harvesting complex stress-related (LHCSR1) protein, which is essential for fast and reversible regulation of light use efficiency in lower plants, as compared to the major LHCII antenna protein, which mainly fulfills light harvesting function. Both LHCII and LHCSR1 had a chlorophyll fluorescence yield and lifetime strongly dependent on detergent concentration but the transition from long- to short-living states was far more complete and fast in the latter. Low pH and zeaxanthin binding enhanced the relative amplitude of quenched states in LHCSR1, which were characterized by the presence of 80 ps fluorescence decay components with a red-shifted emission spectrum. We suggest that energy dissipation occurs in the chloroplast by the activation of 80 ps quenching sites in LHCSR1 which spill over excitons from the photosystem II antenna system. More... »

PAGES

11158

References to SciGraph publications

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  • 2013-04-18. Light harvesting in photosystem II in PHOTOSYNTHESIS RESEARCH
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  • 2004-03. Crystal structure of spinach major light-harvesting complex at 2.72 Å resolution in NATURE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41598-017-11101-7

    DOI

    http://dx.doi.org/10.1038/s41598-017-11101-7

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1091536554

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/28894198


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    42 activation
    43 aim
    44 algae
    45 amplitude
    46 antenna proteins
    47 antenna system
    48 binding
    49 chlorophyll fluorescence yield
    50 chloroplasts
    51 complexes
    52 components
    53 concentration
    54 detergent concentration
    55 dissipation
    56 dynamic properties
    57 efficiency
    58 emission spectra
    59 energy
    60 energy dissipation
    61 excess
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    63 excitons
    64 fluorescence
    65 fluorescence yield
    66 formation
    67 function
    68 functional modulation
    69 functional state
    70 green algae
    71 harmful photoproducts
    72 harvesting
    73 harvesting function
    74 lifetime
    75 light harvesting
    76 light use efficiency
    77 light-harvesting complex stress-related proteins
    78 light-harvesting function
    79 living states
    80 low pH
    81 lower plants
    82 lumen acidification
    83 mechanism
    84 modulation
    85 mosses
    86 oxygenic photosynthesis
    87 pH
    88 photoproducts
    89 photoprotective mechanisms
    90 photosynthesis
    91 photosystem II antenna system
    92 pigments
    93 plants
    94 presence
    95 properties
    96 protein
    97 quenched state
    98 quenching state
    99 red-shifted emission spectra
    100 regulation
    101 relative amplitude
    102 reversible regulation
    103 sites
    104 spectra
    105 spill
    106 state
    107 stress-related proteins
    108 system
    109 transition
    110 triggers
    111 use efficiency
    112 yield
    113 zeaxanthin binding
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