Functional association of cellular microtubules with viral capsid assembly supports efficient hepatitis B virus replication View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-12

AUTHORS

Masashi Iwamoto, Dawei Cai, Masaya Sugiyama, Ryosuke Suzuki, Hideki Aizaki, Akihide Ryo, Naoko Ohtani, Yasuhito Tanaka, Masashi Mizokami, Takaji Wakita, Haitao Guo, Koichi Watashi

ABSTRACT

Viruses exploit host factors and environment for their efficient replication. The virus-host interaction mechanisms for achieving an optimal hepatitis B virus (HBV) replication have been largely unknown. Here, a single cell cloning revealed that HepAD38 cells, a widely-used HBV-inducible cell line, contain cell clones with diverse permissiveness to HBV replication. The HBV permissiveness was impaired upon treatment with microtubule inhibitor nocodazole, which was identified as an HBV replication inhibitor from a pharmacological screening. In the microtubule-disrupted cells, the efficiency of HBV capsid assembly was remarkably decreased without significant change in pre-assembly process. We further found that HBV core interacted with tubulin and co-localized with microtubule-like fibriforms, but this association was abrogated upon microtubule-disassembly agents, resulting in attenuation of capsid formation. Our data thus suggest a significant role of microtubules in the efficient capsid formation during HBV replication. In line with this, a highly HBV permissive cell clone of HepAD38 cells showed a prominent association of core-microtubule and thus a high capacity to support the capsid formation. These findings provide a new aspect of virus-cell interaction for rendering efficient HBV replication. More... »

PAGES

10620

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-017-11015-4

DOI

http://dx.doi.org/10.1038/s41598-017-11015-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1091456860

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28878350


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Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-017-11015-4'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-017-11015-4'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-017-11015-4'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-017-11015-4'


 

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302 https://www.grid.ac/institutes/grid.410795.e schema:alternateName National Institute of Infectious Diseases
303 schema:name Department of Virology II, National Institute of Infectious Diseases, 162-8640, Tokyo, Japan
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305 https://www.grid.ac/institutes/grid.419082.6 schema:alternateName Japan Science and Technology Agency
306 schema:name CREST, JST, 332-0012, Saitama, Japan
307 Department of Applied Biological Sciences, Tokyo University of Science, 278-8510, Noda, Japan
308 Department of Virology II, National Institute of Infectious Diseases, 162-8640, Tokyo, Japan
309 rdf:type schema:Organization
310 https://www.grid.ac/institutes/grid.45203.30 schema:alternateName National Center For Global Health and Medicine
311 schema:name Genome Medical Sciences Project, National Center for Global Health and Medicine, 272-8516, Ichikawa, Japan
312 rdf:type schema:Organization
 




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