Ontology type: schema:ScholarlyArticle Open Access: True
2017-07-26
AUTHORSHye Sung Kim, Cheol Lee, Woo Ho Kim, Young Hee Maeng, Bo Gun Jang
ABSTRACTThe intestinal epithelium has two distinct two stem cell populations, namely, crypt base columnar (CBC) cells and +4 cells. Several specific markers have been identified for each stem cell population. In this study, we examined the expression profiles of these markers in colitis-associated carcinogenesis (CAC) to investigate whether they can be used as biomarkers for the early detection of dysplasia. The expression of intestinal stem cell (ISC) markers was measured by real-time polymerase chain reaction during CAC that was induced by azoxymethane and dextran sodium sulfate treatment. CBC stem cell markers increased continuously with tumor development, whereas a +4 cell expression profile was not present. CBC stem cell population was suppressed in the acute colitis and then expanded to repopulate the crypts during the regeneration period. Notably, RNA in situ hybridization revealed that all dysplasia and cancer samples showed increased expression of CBC stem cell markers in more than one-third of the tumor height, whereas regenerative glands had CBC stem cell markers confined to the lower one-third of the crypt. These results suggest that CBC stem cell markers could be a useful tool for the early detection of colitis-induced tumors. More... »
PAGES6533
http://scigraph.springernature.com/pub.10.1038/s41598-017-06900-x
DOIhttp://dx.doi.org/10.1038/s41598-017-06900-x
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1090836401
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/28747693
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/10",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Technology",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Genetics",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1004",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Medical Biotechnology",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Animals",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Biomarkers, Tumor",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Carcinogenesis",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Colitis",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Disease Models, Animal",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Epithelial Cells",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Gene Expression Profiling",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Humans",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "In Situ Hybridization",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mice, Inbred C57BL",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Real-Time Polymerase Chain Reaction",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Stem Cells",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Department of Pathology, Jeju National University School of Medicine, Jeju, 690-767 Korea",
"id": "http://www.grid.ac/institutes/grid.411277.6",
"name": [
"Department of Pathology, Jeju National University School of Medicine, Jeju, 690-767 Korea"
],
"type": "Organization"
},
"familyName": "Kim",
"givenName": "Hye Sung",
"id": "sg:person.0771453563.33",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0771453563.33"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Pathology, Seoul National University College of Medicine, Seoul, 110-799 Korea",
"id": "http://www.grid.ac/institutes/grid.31501.36",
"name": [
"Department of Pathology, Seoul National University College of Medicine, Seoul, 110-799 Korea"
],
"type": "Organization"
},
"familyName": "Lee",
"givenName": "Cheol",
"id": "sg:person.0634207106.19",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0634207106.19"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Pathology, Seoul National University College of Medicine, Seoul, 110-799 Korea",
"id": "http://www.grid.ac/institutes/grid.31501.36",
"name": [
"Department of Pathology, Seoul National University College of Medicine, Seoul, 110-799 Korea"
],
"type": "Organization"
},
"familyName": "Kim",
"givenName": "Woo Ho",
"id": "sg:person.016630576674.47",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016630576674.47"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Pathology, Jeju National University School of Medicine, Jeju, 690-767 Korea",
"id": "http://www.grid.ac/institutes/grid.411277.6",
"name": [
"Department of Pathology, Jeju National University School of Medicine, Jeju, 690-767 Korea"
],
"type": "Organization"
},
"familyName": "Maeng",
"givenName": "Young Hee",
"id": "sg:person.0662244573.23",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0662244573.23"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Pathology, Jeju National University School of Medicine, Jeju, 690-767 Korea",
"id": "http://www.grid.ac/institutes/grid.411277.6",
"name": [
"Department of Pathology, Jeju National University School of Medicine, Jeju, 690-767 Korea"
],
"type": "Organization"
},
"familyName": "Jang",
"givenName": "Bo Gun",
"id": "sg:person.0640426775.09",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0640426775.09"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1038/269518a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026891740",
"https://doi.org/10.1038/269518a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nrgastro.2009.221",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022388882",
"https://doi.org/10.1038/nrgastro.2009.221"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature07589",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1037637049",
"https://doi.org/10.1038/nature07589"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nrgastro.2009.232",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1020051006",
"https://doi.org/10.1038/nrgastro.2009.232"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nrgastro.2009.44",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1051881178",
"https://doi.org/10.1038/nrgastro.2009.44"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature07602",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1020963310",
"https://doi.org/10.1038/nature07602"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/srep08654",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1049213053",
"https://doi.org/10.1038/srep08654"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/ncb2384",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1033295795",
"https://doi.org/10.1038/ncb2384"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nrm3721",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1051868900",
"https://doi.org/10.1038/nrm3721"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/ng.165",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1025078309",
"https://doi.org/10.1038/ng.165"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature06196",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050225537",
"https://doi.org/10.1038/nature06196"
],
"type": "CreativeWork"
}
],
"datePublished": "2017-07-26",
"datePublishedReg": "2017-07-26",
"description": "The intestinal epithelium has two distinct two stem cell populations, namely, crypt base columnar (CBC) cells and +4 cells. Several specific markers have been identified for each stem cell population. In this study, we examined the expression profiles of these markers in colitis-associated carcinogenesis (CAC) to investigate whether they can be used as biomarkers for the early detection of dysplasia. The expression of intestinal stem cell (ISC) markers was measured by real-time polymerase chain reaction during CAC that was induced by azoxymethane and dextran sodium sulfate treatment. CBC stem cell markers increased continuously with tumor development, whereas a +4 cell expression profile was not present. CBC stem cell population was suppressed in the acute colitis and then expanded to repopulate the crypts during the regeneration period. Notably, RNA in situ hybridization revealed that all dysplasia and cancer samples showed increased expression of CBC stem cell markers in more than one-third of the tumor height, whereas regenerative glands had CBC stem cell markers confined to the lower one-third of the crypt. These results suggest that CBC stem cell markers could be a useful tool for the early detection of colitis-induced tumors.",
"genre": "article",
"id": "sg:pub.10.1038/s41598-017-06900-x",
"inLanguage": "en",
"isAccessibleForFree": true,
"isPartOf": [
{
"id": "sg:journal.1045337",
"issn": [
"2045-2322"
],
"name": "Scientific Reports",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "1",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "7"
}
],
"keywords": [
"colitis-associated carcinogenesis",
"stem cell markers",
"intestinal stem cell markers",
"cell markers",
"cell populations",
"dextran sodium sulfate (DSS) treatment",
"stem cell population",
"early detection",
"sodium sulfate treatment",
"real-time polymerase chain reaction",
"one-third",
"expression profiles",
"crypt base columnar cells",
"regenerative glands",
"acute colitis",
"tumor height",
"polymerase chain reaction",
"intestinal epithelium",
"tumor development",
"cancer samples",
"columnar cells",
"specific markers",
"chain reaction",
"dysplasia",
"cell expression profiles",
"markers",
"carcinogenesis",
"situ hybridization",
"crypts",
"sulfate treatment",
"detection",
"population",
"colitis",
"cells",
"azoxymethane",
"expression",
"tumors",
"epithelium",
"biomarkers",
"gland",
"useful tool",
"treatment",
"profile",
"regeneration period",
"RNA",
"period",
"study",
"hybridization",
"reaction",
"samples",
"development",
"results",
"tool",
"height",
"base columnar (CBC) cells",
"CBC stem cell markers",
"CBC stem cell population",
"colitis-induced tumors"
],
"name": "Expression profile of intestinal stem cell markers in colitis-associated carcinogenesis",
"pagination": "6533",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1090836401"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1038/s41598-017-06900-x"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"28747693"
]
}
],
"sameAs": [
"https://doi.org/10.1038/s41598-017-06900-x",
"https://app.dimensions.ai/details/publication/pub.1090836401"
],
"sdDataset": "articles",
"sdDatePublished": "2022-01-01T18:45",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_752.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1038/s41598-017-06900-x"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-017-06900-x'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-017-06900-x'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-017-06900-x'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-017-06900-x'
This table displays all metadata directly associated to this object as RDF triples.
250 TRIPLES
22 PREDICATES
109 URIs
88 LITERALS
19 BLANK NODES