Mass spectrometry-based determination of Kallikrein-related peptidase 7 (KLK7) cleavage preferences and subsite dependency View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-12

AUTHORS

Lakmali Munasinghage Silva, Thomas Stoll, Thomas Kryza, Carson Ryan Stephens, Marcus Lachlan Hastie, Helen Frances Irving-Rodgers, Ying Dong, Jeffrey John Gorman, Judith Ann Clements

ABSTRACT

The cleavage preferences of Kallikrein-related peptidase 7 (KLK7) have previously been delineated using synthetic peptide libraries of fixed length, or single protein chains and have suggested that KLK7 exerts a chymotryptic-like cleavage preference. Due to the short length of the peptides utilised, only a limited number of subsites have however been assessed. To determine the subsite preferences of KLK7 in a global setting, we used a mass spectrometry (MS)-based in-depth proteomics approach that utilises human proteome-derived peptide libraries of varying length, termed Proteomic Identification of protease Cleavage Sites (PICS). Consistent with previous findings, KLK7 was found to exert chymotryptic-like cleavage preferences. KLK7 subsite preferences were also characterised in the P2-P2' region, demonstrating a preference for hydrophobic residues in the non-prime and hydrophilic residues in the prime subsites. Interestingly, single catalytic triad mutant KLK7 (mKLK7; S195A) also showed residual catalytic activity (kcat/KM = 7.93 × 102 s-1M-1). Catalytic inactivity of KLK7 was however achieved by additional mutation in this region (D102N). In addition to characterising the cleavage preferences of KLK7, our data thereby also suggests that the use of double catalytic triad mutants should be employed as more appropriate negative controls in future investigations of KLK7, especially when highly sensitive MS-based approaches are employed. More... »

PAGES

6789

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-017-06680-4

DOI

http://dx.doi.org/10.1038/s41598-017-06680-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1090882953

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28754951


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "National Institute of Dental and Craniofacial Research", 
          "id": "https://www.grid.ac/institutes/grid.419633.a", 
          "name": [
            "Queensland University of Technology (QUT), Institute of Health and Biomedical Innovation (IHBI) and School of Biomedical Sciences at the Translational Research Institute, 37 Kent Street, 4102, Woolloongabba, Queensland, Australia", 
            "Proteases and Tissue Remodelling Section, National Institute of Dental and Craniofacial Research, National Institutes of Science, 30 Convent Drive, 20892, Bethesda, Maryland, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Silva", 
        "givenName": "Lakmali Munasinghage", 
        "id": "sg:person.016267143117.67", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016267143117.67"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "QIMR Berghofer Medical Research Institute", 
          "id": "https://www.grid.ac/institutes/grid.1049.c", 
          "name": [
            "QIMR Berghofer Medical Research Institute, 300 Herston Road, 4006, Herston, Queensland, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Stoll", 
        "givenName": "Thomas", 
        "id": "sg:person.0622526127.93", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0622526127.93"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Queensland University of Technology", 
          "id": "https://www.grid.ac/institutes/grid.1024.7", 
          "name": [
            "Queensland University of Technology (QUT), Institute of Health and Biomedical Innovation (IHBI) and School of Biomedical Sciences at the Translational Research Institute, 37 Kent Street, 4102, Woolloongabba, Queensland, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kryza", 
        "givenName": "Thomas", 
        "id": "sg:person.0774665761.20", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0774665761.20"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Queensland University of Technology", 
          "id": "https://www.grid.ac/institutes/grid.1024.7", 
          "name": [
            "Queensland University of Technology (QUT), Institute of Health and Biomedical Innovation (IHBI) and School of Biomedical Sciences at the Translational Research Institute, 37 Kent Street, 4102, Woolloongabba, Queensland, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Stephens", 
        "givenName": "Carson Ryan", 
        "id": "sg:person.01337101662.66", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01337101662.66"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "QIMR Berghofer Medical Research Institute", 
          "id": "https://www.grid.ac/institutes/grid.1049.c", 
          "name": [
            "QIMR Berghofer Medical Research Institute, 300 Herston Road, 4006, Herston, Queensland, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hastie", 
        "givenName": "Marcus Lachlan", 
        "id": "sg:person.0633042373.26", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0633042373.26"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Griffith University", 
          "id": "https://www.grid.ac/institutes/grid.1022.1", 
          "name": [
            "School of Medical Science, Griffith University Gold Coast Campus, Parklands Drive, 4215, Southport, Queensland, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Irving-Rodgers", 
        "givenName": "Helen Frances", 
        "id": "sg:person.01235155730.12", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01235155730.12"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Queensland University of Technology", 
          "id": "https://www.grid.ac/institutes/grid.1024.7", 
          "name": [
            "Queensland University of Technology (QUT), Institute of Health and Biomedical Innovation (IHBI) and School of Biomedical Sciences at the Translational Research Institute, 37 Kent Street, 4102, Woolloongabba, Queensland, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Dong", 
        "givenName": "Ying", 
        "id": "sg:person.01270766462.55", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270766462.55"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "QIMR Berghofer Medical Research Institute", 
          "id": "https://www.grid.ac/institutes/grid.1049.c", 
          "name": [
            "QIMR Berghofer Medical Research Institute, 300 Herston Road, 4006, Herston, Queensland, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gorman", 
        "givenName": "Jeffrey John", 
        "id": "sg:person.011577041157.83", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011577041157.83"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Queensland University of Technology", 
          "id": "https://www.grid.ac/institutes/grid.1024.7", 
          "name": [
            "Queensland University of Technology (QUT), Institute of Health and Biomedical Innovation (IHBI) and School of Biomedical Sciences at the Translational Research Institute, 37 Kent Street, 4102, Woolloongabba, Queensland, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Clements", 
        "givenName": "Judith Ann", 
        "id": "sg:person.015357417702.11", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015357417702.11"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/s1043-2760(99)00225-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000798017"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1515/hsz-2014-0140", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003632205"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/b978-012249340-9/50007-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003745962"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molonc.2013.09.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004009574"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0378-1119(00)00280-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007320987"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ygyno.2012.09.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009219003"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m602372200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009811965"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0707811104", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014415256"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nprot.2010.178", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016176838", 
          "https://doi.org/10.1038/nprot.2010.178"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.271.36.21886", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018802382"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biochi.2010.06.022", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022958545"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m705190200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024181263"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bip.22231", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024362018"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0968-0004(88)90121-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025548374"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0968-0004(88)90121-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025548374"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jid.2016.09.017", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026604669"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1515/bc.2008.075", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027911455"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1515/bc.2011.158", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031440014"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m709493200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034533941"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbapap.2014.10.018", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037842484"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/10408360490471931", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039242130"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1515/bc.2001.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039588010"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/bbrc.1995.1853", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040341241"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/332564a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040472721", 
          "https://doi.org/10.1038/332564a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/0008-5472.can-09-3415", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041440166"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt1408", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041621508", 
          "https://doi.org/10.1038/nbt1408"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0006-291x(67)80055-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042897284"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1470-8744.1999.tb00770.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046776813"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1096/fj.06-6075com", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047616961"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/acschembio.5b01051", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055135131"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja00031a037", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055700574"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.3299704", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062604927"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.3303334", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062606014"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1210/er.2009-0034", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1064285977"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075283508", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2144/04361dd02", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1076629359"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2017-12", 
    "datePublishedReg": "2017-12-01", 
    "description": "The cleavage preferences of Kallikrein-related peptidase 7 (KLK7) have previously been delineated using synthetic peptide libraries of fixed length, or single protein chains and have suggested that KLK7 exerts a chymotryptic-like cleavage preference. Due to the short length of the peptides utilised, only a limited number of subsites have however been assessed. To determine the subsite preferences of KLK7 in a global setting, we used a mass spectrometry (MS)-based in-depth proteomics approach that utilises human proteome-derived peptide libraries of varying length, termed Proteomic Identification of protease Cleavage Sites (PICS). Consistent with previous findings, KLK7 was found to exert chymotryptic-like cleavage preferences. KLK7 subsite preferences were also characterised in the P2-P2' region, demonstrating a preference for hydrophobic residues in the non-prime and hydrophilic residues in the prime subsites. Interestingly, single catalytic triad mutant KLK7 (mKLK7; S195A) also showed residual catalytic activity (kcat/KM\u2009=\u20097.93\u2009\u00d7\u2009102\u2009s-1M-1). Catalytic inactivity of KLK7 was however achieved by additional mutation in this region (D102N). In addition to characterising the cleavage preferences of KLK7, our data thereby also suggests that the use of double catalytic triad mutants should be employed as more appropriate negative controls in future investigations of KLK7, especially when highly sensitive MS-based approaches are employed.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/s41598-017-06680-4", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.6712910", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.6712661", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1045337", 
        "issn": [
          "2045-2322"
        ], 
        "name": "Scientific Reports", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "7"
      }
    ], 
    "name": "Mass spectrometry-based determination of Kallikrein-related peptidase 7 (KLK7) cleavage preferences and subsite dependency", 
    "pagination": "6789", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "9827c278d5d17cfdb53c91008b1cdebc41d625e473341d4f5cd6fe9d319a1285"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "28754951"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101563288"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/s41598-017-06680-4"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1090882953"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/s41598-017-06680-4", 
      "https://app.dimensions.ai/details/publication/pub.1090882953"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T18:16", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8675_00000493.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/s41598-017-06680-4"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-017-06680-4'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-017-06680-4'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-017-06680-4'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-017-06680-4'


 

This table displays all metadata directly associated to this object as RDF triples.

245 TRIPLES      21 PREDICATES      64 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/s41598-017-06680-4 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N70e9a04da0414ddd987ec25137e7da22
4 schema:citation sg:pub.10.1038/332564a0
5 sg:pub.10.1038/nbt1408
6 sg:pub.10.1038/nprot.2010.178
7 https://app.dimensions.ai/details/publication/pub.1075283508
8 https://doi.org/10.1002/bip.22231
9 https://doi.org/10.1006/bbrc.1995.1853
10 https://doi.org/10.1016/0968-0004(88)90121-1
11 https://doi.org/10.1016/b978-012249340-9/50007-3
12 https://doi.org/10.1016/j.bbapap.2014.10.018
13 https://doi.org/10.1016/j.biochi.2010.06.022
14 https://doi.org/10.1016/j.jid.2016.09.017
15 https://doi.org/10.1016/j.molonc.2013.09.003
16 https://doi.org/10.1016/j.ygyno.2012.09.001
17 https://doi.org/10.1016/s0006-291x(67)80055-x
18 https://doi.org/10.1016/s0378-1119(00)00280-8
19 https://doi.org/10.1016/s1043-2760(99)00225-8
20 https://doi.org/10.1021/acschembio.5b01051
21 https://doi.org/10.1021/ja00031a037
22 https://doi.org/10.1073/pnas.0707811104
23 https://doi.org/10.1074/jbc.271.36.21886
24 https://doi.org/10.1074/jbc.m602372200
25 https://doi.org/10.1074/jbc.m705190200
26 https://doi.org/10.1074/jbc.m709493200
27 https://doi.org/10.1080/10408360490471931
28 https://doi.org/10.1096/fj.06-6075com
29 https://doi.org/10.1111/j.1470-8744.1999.tb00770.x
30 https://doi.org/10.1126/science.3299704
31 https://doi.org/10.1126/science.3303334
32 https://doi.org/10.1158/0008-5472.can-09-3415
33 https://doi.org/10.1210/er.2009-0034
34 https://doi.org/10.1515/bc.2001.002
35 https://doi.org/10.1515/bc.2008.075
36 https://doi.org/10.1515/bc.2011.158
37 https://doi.org/10.1515/hsz-2014-0140
38 https://doi.org/10.2144/04361dd02
39 schema:datePublished 2017-12
40 schema:datePublishedReg 2017-12-01
41 schema:description The cleavage preferences of Kallikrein-related peptidase 7 (KLK7) have previously been delineated using synthetic peptide libraries of fixed length, or single protein chains and have suggested that KLK7 exerts a chymotryptic-like cleavage preference. Due to the short length of the peptides utilised, only a limited number of subsites have however been assessed. To determine the subsite preferences of KLK7 in a global setting, we used a mass spectrometry (MS)-based in-depth proteomics approach that utilises human proteome-derived peptide libraries of varying length, termed Proteomic Identification of protease Cleavage Sites (PICS). Consistent with previous findings, KLK7 was found to exert chymotryptic-like cleavage preferences. KLK7 subsite preferences were also characterised in the P2-P2' region, demonstrating a preference for hydrophobic residues in the non-prime and hydrophilic residues in the prime subsites. Interestingly, single catalytic triad mutant KLK7 (mKLK7; S195A) also showed residual catalytic activity (k<sub>cat</sub>/K<sub>M</sub> = 7.93 × 10<sup>2</sup> s<sup>-1</sup>M<sup>-1</sup>). Catalytic inactivity of KLK7 was however achieved by additional mutation in this region (D102N). In addition to characterising the cleavage preferences of KLK7, our data thereby also suggests that the use of double catalytic triad mutants should be employed as more appropriate negative controls in future investigations of KLK7, especially when highly sensitive MS-based approaches are employed.
42 schema:genre research_article
43 schema:inLanguage en
44 schema:isAccessibleForFree true
45 schema:isPartOf N2f12516b109f428aa30b03faf821034b
46 Nb042bf01d5844787be782459f2a00e52
47 sg:journal.1045337
48 schema:name Mass spectrometry-based determination of Kallikrein-related peptidase 7 (KLK7) cleavage preferences and subsite dependency
49 schema:pagination 6789
50 schema:productId N65ba9cbc35ea43738174f5b05affec44
51 N76d77b974c9e462f94bbaf1ddf8f68f5
52 N86972ecfaed5480a95ac1aaf357a0a2f
53 N8aa82f32cbd5455584f05c0b97a1e48d
54 Nc5b586a73c1d43529769bffc66cf8631
55 schema:sameAs https://app.dimensions.ai/details/publication/pub.1090882953
56 https://doi.org/10.1038/s41598-017-06680-4
57 schema:sdDatePublished 2019-04-10T18:16
58 schema:sdLicense https://scigraph.springernature.com/explorer/license/
59 schema:sdPublisher Ne467a43de19a4ad7a894744276e33a3b
60 schema:url https://www.nature.com/articles/s41598-017-06680-4
61 sgo:license sg:explorer/license/
62 sgo:sdDataset articles
63 rdf:type schema:ScholarlyArticle
64 N0bcf3334b9e14b52b36fcdb55321ee18 rdf:first sg:person.01337101662.66
65 rdf:rest N5c57cf3f9ecc42669492a755d0fd66a4
66 N0cda70517dc94e8eaf791d00a605ae3c rdf:first sg:person.0774665761.20
67 rdf:rest N0bcf3334b9e14b52b36fcdb55321ee18
68 N2cb1df782ae142b4b1ec641f49f3040d rdf:first sg:person.01270766462.55
69 rdf:rest Nea683a46317a4be79d07c50f09eab1e6
70 N2f12516b109f428aa30b03faf821034b schema:issueNumber 1
71 rdf:type schema:PublicationIssue
72 N30f45412ca274079b651dd0ddfa31ab5 rdf:first sg:person.01235155730.12
73 rdf:rest N2cb1df782ae142b4b1ec641f49f3040d
74 N5c57cf3f9ecc42669492a755d0fd66a4 rdf:first sg:person.0633042373.26
75 rdf:rest N30f45412ca274079b651dd0ddfa31ab5
76 N65ba9cbc35ea43738174f5b05affec44 schema:name nlm_unique_id
77 schema:value 101563288
78 rdf:type schema:PropertyValue
79 N6cc7df933e764252b06d3489d7e56b00 rdf:first sg:person.015357417702.11
80 rdf:rest rdf:nil
81 N70e9a04da0414ddd987ec25137e7da22 rdf:first sg:person.016267143117.67
82 rdf:rest Nf2927a705d014f03b054a5b07a6228b2
83 N76d77b974c9e462f94bbaf1ddf8f68f5 schema:name doi
84 schema:value 10.1038/s41598-017-06680-4
85 rdf:type schema:PropertyValue
86 N86972ecfaed5480a95ac1aaf357a0a2f schema:name pubmed_id
87 schema:value 28754951
88 rdf:type schema:PropertyValue
89 N8aa82f32cbd5455584f05c0b97a1e48d schema:name dimensions_id
90 schema:value pub.1090882953
91 rdf:type schema:PropertyValue
92 Nb042bf01d5844787be782459f2a00e52 schema:volumeNumber 7
93 rdf:type schema:PublicationVolume
94 Nc5b586a73c1d43529769bffc66cf8631 schema:name readcube_id
95 schema:value 9827c278d5d17cfdb53c91008b1cdebc41d625e473341d4f5cd6fe9d319a1285
96 rdf:type schema:PropertyValue
97 Ne467a43de19a4ad7a894744276e33a3b schema:name Springer Nature - SN SciGraph project
98 rdf:type schema:Organization
99 Nea683a46317a4be79d07c50f09eab1e6 rdf:first sg:person.011577041157.83
100 rdf:rest N6cc7df933e764252b06d3489d7e56b00
101 Nf2927a705d014f03b054a5b07a6228b2 rdf:first sg:person.0622526127.93
102 rdf:rest N0cda70517dc94e8eaf791d00a605ae3c
103 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
104 schema:name Biological Sciences
105 rdf:type schema:DefinedTerm
106 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
107 schema:name Biochemistry and Cell Biology
108 rdf:type schema:DefinedTerm
109 sg:grant.6712661 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-017-06680-4
110 rdf:type schema:MonetaryGrant
111 sg:grant.6712910 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-017-06680-4
112 rdf:type schema:MonetaryGrant
113 sg:journal.1045337 schema:issn 2045-2322
114 schema:name Scientific Reports
115 rdf:type schema:Periodical
116 sg:person.011577041157.83 schema:affiliation https://www.grid.ac/institutes/grid.1049.c
117 schema:familyName Gorman
118 schema:givenName Jeffrey John
119 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011577041157.83
120 rdf:type schema:Person
121 sg:person.01235155730.12 schema:affiliation https://www.grid.ac/institutes/grid.1022.1
122 schema:familyName Irving-Rodgers
123 schema:givenName Helen Frances
124 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01235155730.12
125 rdf:type schema:Person
126 sg:person.01270766462.55 schema:affiliation https://www.grid.ac/institutes/grid.1024.7
127 schema:familyName Dong
128 schema:givenName Ying
129 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270766462.55
130 rdf:type schema:Person
131 sg:person.01337101662.66 schema:affiliation https://www.grid.ac/institutes/grid.1024.7
132 schema:familyName Stephens
133 schema:givenName Carson Ryan
134 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01337101662.66
135 rdf:type schema:Person
136 sg:person.015357417702.11 schema:affiliation https://www.grid.ac/institutes/grid.1024.7
137 schema:familyName Clements
138 schema:givenName Judith Ann
139 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015357417702.11
140 rdf:type schema:Person
141 sg:person.016267143117.67 schema:affiliation https://www.grid.ac/institutes/grid.419633.a
142 schema:familyName Silva
143 schema:givenName Lakmali Munasinghage
144 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016267143117.67
145 rdf:type schema:Person
146 sg:person.0622526127.93 schema:affiliation https://www.grid.ac/institutes/grid.1049.c
147 schema:familyName Stoll
148 schema:givenName Thomas
149 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0622526127.93
150 rdf:type schema:Person
151 sg:person.0633042373.26 schema:affiliation https://www.grid.ac/institutes/grid.1049.c
152 schema:familyName Hastie
153 schema:givenName Marcus Lachlan
154 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0633042373.26
155 rdf:type schema:Person
156 sg:person.0774665761.20 schema:affiliation https://www.grid.ac/institutes/grid.1024.7
157 schema:familyName Kryza
158 schema:givenName Thomas
159 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0774665761.20
160 rdf:type schema:Person
161 sg:pub.10.1038/332564a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040472721
162 https://doi.org/10.1038/332564a0
163 rdf:type schema:CreativeWork
164 sg:pub.10.1038/nbt1408 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041621508
165 https://doi.org/10.1038/nbt1408
166 rdf:type schema:CreativeWork
167 sg:pub.10.1038/nprot.2010.178 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016176838
168 https://doi.org/10.1038/nprot.2010.178
169 rdf:type schema:CreativeWork
170 https://app.dimensions.ai/details/publication/pub.1075283508 schema:CreativeWork
171 https://doi.org/10.1002/bip.22231 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024362018
172 rdf:type schema:CreativeWork
173 https://doi.org/10.1006/bbrc.1995.1853 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040341241
174 rdf:type schema:CreativeWork
175 https://doi.org/10.1016/0968-0004(88)90121-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025548374
176 rdf:type schema:CreativeWork
177 https://doi.org/10.1016/b978-012249340-9/50007-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003745962
178 rdf:type schema:CreativeWork
179 https://doi.org/10.1016/j.bbapap.2014.10.018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037842484
180 rdf:type schema:CreativeWork
181 https://doi.org/10.1016/j.biochi.2010.06.022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022958545
182 rdf:type schema:CreativeWork
183 https://doi.org/10.1016/j.jid.2016.09.017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026604669
184 rdf:type schema:CreativeWork
185 https://doi.org/10.1016/j.molonc.2013.09.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004009574
186 rdf:type schema:CreativeWork
187 https://doi.org/10.1016/j.ygyno.2012.09.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009219003
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1016/s0006-291x(67)80055-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1042897284
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1016/s0378-1119(00)00280-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007320987
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1016/s1043-2760(99)00225-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000798017
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1021/acschembio.5b01051 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055135131
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1021/ja00031a037 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055700574
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1073/pnas.0707811104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014415256
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1074/jbc.271.36.21886 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018802382
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1074/jbc.m602372200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009811965
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1074/jbc.m705190200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024181263
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1074/jbc.m709493200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034533941
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1080/10408360490471931 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039242130
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1096/fj.06-6075com schema:sameAs https://app.dimensions.ai/details/publication/pub.1047616961
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1111/j.1470-8744.1999.tb00770.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1046776813
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1126/science.3299704 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062604927
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1126/science.3303334 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062606014
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1158/0008-5472.can-09-3415 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041440166
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1210/er.2009-0034 schema:sameAs https://app.dimensions.ai/details/publication/pub.1064285977
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1515/bc.2001.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039588010
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1515/bc.2008.075 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027911455
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1515/bc.2011.158 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031440014
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1515/hsz-2014-0140 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003632205
230 rdf:type schema:CreativeWork
231 https://doi.org/10.2144/04361dd02 schema:sameAs https://app.dimensions.ai/details/publication/pub.1076629359
232 rdf:type schema:CreativeWork
233 https://www.grid.ac/institutes/grid.1022.1 schema:alternateName Griffith University
234 schema:name School of Medical Science, Griffith University Gold Coast Campus, Parklands Drive, 4215, Southport, Queensland, Australia
235 rdf:type schema:Organization
236 https://www.grid.ac/institutes/grid.1024.7 schema:alternateName Queensland University of Technology
237 schema:name Queensland University of Technology (QUT), Institute of Health and Biomedical Innovation (IHBI) and School of Biomedical Sciences at the Translational Research Institute, 37 Kent Street, 4102, Woolloongabba, Queensland, Australia
238 rdf:type schema:Organization
239 https://www.grid.ac/institutes/grid.1049.c schema:alternateName QIMR Berghofer Medical Research Institute
240 schema:name QIMR Berghofer Medical Research Institute, 300 Herston Road, 4006, Herston, Queensland, Australia
241 rdf:type schema:Organization
242 https://www.grid.ac/institutes/grid.419633.a schema:alternateName National Institute of Dental and Craniofacial Research
243 schema:name Proteases and Tissue Remodelling Section, National Institute of Dental and Craniofacial Research, National Institutes of Science, 30 Convent Drive, 20892, Bethesda, Maryland, USA
244 Queensland University of Technology (QUT), Institute of Health and Biomedical Innovation (IHBI) and School of Biomedical Sciences at the Translational Research Institute, 37 Kent Street, 4102, Woolloongabba, Queensland, Australia
245 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...