Hypersaline sapropels act as hotspots for microbial dark matter View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-07-21

AUTHORS

Adrian-Ştefan Andrei, Andreea Baricz, Michael Scott Robeson, Manuela Raluca Păuşan, Tudor Tămaş, Cecilia Chiriac, Edina Szekeres, Lucian Barbu-Tudoran, Erika Andrea Levei, Cristian Coman, Mircea Podar, Horia Leonard Banciu

ABSTRACT

Present-day terrestrial analogue sites are crucial ground truth proxies for studying life in geochemical conditions close to those assumed to be present on early Earth or inferred to exist on other celestial bodies (e.g. Mars, Europa). Although hypersaline sapropels are border-of-life habitats with moderate occurrence, their microbiological and physicochemical characterization lags behind. Here, we study the diversity of life under low water activity by describing the prokaryotic communities from two disparate hypersaline sapropels (Transylvanian Basin, Romania) in relation to geochemical milieu and pore water chemistry, while inferring their role in carbon cycling by matching taxa to known taxon-specific biogeochemical functions. The polyphasic approach combined deep coverage SSU rRNA gene amplicon sequencing and bioinformatics with RT-qPCR and physicochemical investigations. We found that sapropels developed an analogous elemental milieu and harbored prokaryotes affiliated with fifty-nine phyla, among which the most abundant were Proteobacteria, Bacteroidetes and Chloroflexi. Containing thirty-two candidate divisions and possibly undocumented prokaryotic lineages, the hypersaline sapropels were found to accommodate one of the most diverse and novel ecosystems reported to date and may contribute to completing the phylogenetic branching of the tree of life. More... »

PAGES

6150

References to SciGraph publications

  • 2010-04-11. QIIME allows analysis of high-throughput community sequencing data in NATURE METHODS
  • 2015-05-01. Contrasting taxonomic stratification of microbial communities in two hypersaline meromictic lakes in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • 2015-04-13. Transient liquid water and water activity at Gale crater on Mars in NATURE GEOSCIENCE
  • 2003-05-13. Simultaneous Measurements of Organic Carbon Mineralization and Bacterial Production in Oxic and Anoxic Lake Sediments in MICROBIAL ECOLOGY
  • 2011-12-01. An improved Greengenes taxonomy with explicit ranks for ecological and evolutionary analyses of bacteria and archaea in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • 2013-08-18. UPARSE: highly accurate OTU sequences from microbial amplicon reads in NATURE METHODS
  • 2011-06-30. Impact of training sets on classification of high-throughput bacterial 16s rRNA gene surveys in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • 2015-02-14. Culturable diversity of aerobic halophilic archaea (Fam. Halobacteriaceae) from hypersaline, meromictic Transylvanian lakes in EXTREMOPHILES
  • 2014-07-27. Diversity of cultivable halophilic archaea and bacteria from superficial hypersaline sediments of Tunisian solar salterns in ANTONIE VAN LEEUWENHOEK
  • 1993-12. The protection and management of saline lakes of therapeutic value in Romania in HYDROBIOLOGIA
  • 2009-01-24. Characterization of Bacterial Communities Associated with Organic Aggregates in a Large, Shallow, Eutrophic Freshwater Lake (Lake Taihu, China) in MICROBIAL ECOLOGY
  • 2013-09-01. Practical innovations for high-throughput amplicon sequencing in NATURE METHODS
  • 2010-02-04. Shifts in microbial community structure along an ecological gradient of hypersaline soils and sediments in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • 2012-03-07. Preservation of organic matter in sediments promoted by iron in NATURE
  • 2013-09-12. Genomic properties of Marine Group A bacteria indicate a role in the marine sulfur cycle in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • 2009-02-22. Microbial dissolution of clay minerals as a source of iron and silica in marine sediments in NATURE GEOSCIENCE
  • 2012-06-28. Salt marsh sediment diversity: a test of the variability of the rare biosphere among environmental replicates in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41598-017-06232-w

    DOI

    http://dx.doi.org/10.1038/s41598-017-06232-w

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1090754905

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/28733590


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/04", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Earth Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0402", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Geochemistry", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Archaea", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Bacteroidetes", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Benzopyrans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Chloroflexi", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA, Bacterial", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA, Ribosomal", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Geologic Sediments", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humic Substances", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Lakes", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Phylogeny", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Proteobacteria", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Romania", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Sequence Analysis, DNA", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Institute of Hydrobiology, Department of Aquatic Microbial Ecology, Biology Center of the Academy of Sciences of the Czech Republic, \u010cesk\u00e9 Bud\u011bjovice, Czech Republic", 
              "id": "http://www.grid.ac/institutes/grid.448010.9", 
              "name": [
                "Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babe\u015f-Bolyai University, Cluj-Napoca, Romania", 
                "Institute of Hydrobiology, Department of Aquatic Microbial Ecology, Biology Center of the Academy of Sciences of the Czech Republic, \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Andrei", 
            "givenName": "Adrian-\u015etefan", 
            "id": "sg:person.01003205261.19", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01003205261.19"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "National Institute of Research and Development for Biological Sciences (NIRDBS), Institute of Biological Research, Cluj-Napoca, Romania", 
              "id": "http://www.grid.ac/institutes/grid.435400.6", 
              "name": [
                "National Institute of Research and Development for Biological Sciences (NIRDBS), Institute of Biological Research, Cluj-Napoca, Romania"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Baricz", 
            "givenName": "Andreea", 
            "id": "sg:person.01153051752.31", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01153051752.31"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Interstitial Genomics, LLC, Longmont, 80501 Colorado USA", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee USA", 
                "Interstitial Genomics, LLC, Longmont, 80501 Colorado USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Robeson", 
            "givenName": "Michael Scott", 
            "id": "sg:person.01040621745.31", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01040621745.31"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department for Internal Medicine, Medical University of Graz, Graz, Austria", 
              "id": "http://www.grid.ac/institutes/grid.11598.34", 
              "name": [
                "Department for Internal Medicine, Medical University of Graz, Graz, Austria"
              ], 
              "type": "Organization"
            }, 
            "familyName": "P\u0103u\u015fan", 
            "givenName": "Manuela Raluca", 
            "id": "sg:person.0660122652.37", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0660122652.37"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Geology, Faculty of Biology and Geology, Babe\u015f-Bolyai University, Cluj-Napoca, Romania", 
              "id": "http://www.grid.ac/institutes/grid.7399.4", 
              "name": [
                "Department of Geology, Faculty of Biology and Geology, Babe\u015f-Bolyai University, Cluj-Napoca, Romania"
              ], 
              "type": "Organization"
            }, 
            "familyName": "T\u0103ma\u015f", 
            "givenName": "Tudor", 
            "id": "sg:person.014656472653.37", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014656472653.37"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "National Institute of Research and Development for Biological Sciences (NIRDBS), Institute of Biological Research, Cluj-Napoca, Romania", 
              "id": "http://www.grid.ac/institutes/grid.435400.6", 
              "name": [
                "Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babe\u015f-Bolyai University, Cluj-Napoca, Romania", 
                "National Institute of Research and Development for Biological Sciences (NIRDBS), Institute of Biological Research, Cluj-Napoca, Romania"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Chiriac", 
            "givenName": "Cecilia", 
            "id": "sg:person.01113764741.65", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01113764741.65"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "National Institute of Research and Development for Biological Sciences (NIRDBS), Institute of Biological Research, Cluj-Napoca, Romania", 
              "id": "http://www.grid.ac/institutes/grid.435400.6", 
              "name": [
                "Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babe\u015f-Bolyai University, Cluj-Napoca, Romania", 
                "National Institute of Research and Development for Biological Sciences (NIRDBS), Institute of Biological Research, Cluj-Napoca, Romania"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Szekeres", 
            "givenName": "Edina", 
            "id": "sg:person.01151242114.36", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01151242114.36"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babe\u015f-Bolyai University, Cluj-Napoca, Romania", 
              "id": "http://www.grid.ac/institutes/grid.7399.4", 
              "name": [
                "Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babe\u015f-Bolyai University, Cluj-Napoca, Romania"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Barbu-Tudoran", 
            "givenName": "Lucian", 
            "id": "sg:person.01165653363.64", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01165653363.64"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "INCDO-INOE 2000, Research Institute for Analytical Instrumentation, Cluj-Napoca, Romania", 
              "id": "http://www.grid.ac/institutes/grid.483232.c", 
              "name": [
                "INCDO-INOE 2000, Research Institute for Analytical Instrumentation, Cluj-Napoca, Romania"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Levei", 
            "givenName": "Erika Andrea", 
            "id": "sg:person.01037500277.10", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01037500277.10"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "National Institute of Research and Development for Biological Sciences (NIRDBS), Institute of Biological Research, Cluj-Napoca, Romania", 
              "id": "http://www.grid.ac/institutes/grid.435400.6", 
              "name": [
                "National Institute of Research and Development for Biological Sciences (NIRDBS), Institute of Biological Research, Cluj-Napoca, Romania"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Coman", 
            "givenName": "Cristian", 
            "id": "sg:person.0614207202.10", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0614207202.10"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee USA", 
              "id": "http://www.grid.ac/institutes/grid.135519.a", 
              "name": [
                "Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Podar", 
            "givenName": "Mircea", 
            "id": "sg:person.01334660037.45", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01334660037.45"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babe\u015f-Bolyai University, Cluj-Napoca, Romania", 
              "id": "http://www.grid.ac/institutes/grid.7399.4", 
              "name": [
                "Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babe\u015f-Bolyai University, Cluj-Napoca, Romania"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Banciu", 
            "givenName": "Horia Leonard", 
            "id": "sg:person.01170717014.39", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01170717014.39"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1007/s00248-008-9482-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038922548", 
              "https://doi.org/10.1007/s00248-008-9482-8"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.2634", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039971505", 
              "https://doi.org/10.1038/nmeth.2634"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ismej.2012.47", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044196707", 
              "https://doi.org/10.1038/ismej.2012.47"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00792-015-0738-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051387523", 
              "https://doi.org/10.1007/s00792-015-0738-1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10482-014-0238-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006165818", 
              "https://doi.org/10.1007/s10482-014-0238-9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ngeo441", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039147523", 
              "https://doi.org/10.1038/ngeo441"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ismej.2010.3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019352157", 
              "https://doi.org/10.1038/ismej.2010.3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00248-002-1061-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034162851", 
              "https://doi.org/10.1007/s00248-002-1061-9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.f.303", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009032055", 
              "https://doi.org/10.1038/nmeth.f.303"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ismej.2013.152", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035056123", 
              "https://doi.org/10.1038/ismej.2013.152"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.2604", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027743851", 
              "https://doi.org/10.1038/nmeth.2604"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ismej.2011.139", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051863807", 
              "https://doi.org/10.1038/ismej.2011.139"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02905908", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017837825", 
              "https://doi.org/10.1007/bf02905908"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ismej.2015.60", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015256313", 
              "https://doi.org/10.1038/ismej.2015.60"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature10855", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032561330", 
              "https://doi.org/10.1038/nature10855"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ismej.2011.82", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033211760", 
              "https://doi.org/10.1038/ismej.2011.82"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ngeo2412", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029203295", 
              "https://doi.org/10.1038/ngeo2412"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2017-07-21", 
        "datePublishedReg": "2017-07-21", 
        "description": "Present-day terrestrial analogue sites are crucial ground truth proxies for studying life in geochemical conditions close to those assumed to be present on early Earth or inferred to exist on other celestial bodies (e.g. Mars, Europa). Although hypersaline sapropels are border-of-life habitats with moderate occurrence, their microbiological and physicochemical characterization lags behind. Here, we study the diversity of life under low water activity by describing the prokaryotic communities from two disparate hypersaline sapropels (Transylvanian Basin, Romania) in relation to geochemical milieu and pore water chemistry, while inferring their role in carbon cycling by matching taxa to known taxon-specific biogeochemical functions. The polyphasic approach combined deep coverage SSU rRNA gene amplicon sequencing and bioinformatics with RT-qPCR and physicochemical investigations. We found that sapropels developed an analogous elemental milieu and harbored prokaryotes affiliated with fifty-nine phyla, among which the most abundant were Proteobacteria, Bacteroidetes and Chloroflexi. Containing thirty-two candidate divisions and possibly undocumented prokaryotic lineages, the hypersaline sapropels were found to accommodate one of the most diverse and novel ecosystems reported to date and may contribute to completing the phylogenetic branching of the tree of life.", 
        "genre": "article", 
        "id": "sg:pub.10.1038/s41598-017-06232-w", 
        "inLanguage": "en", 
        "isAccessibleForFree": true, 
        "isPartOf": [
          {
            "id": "sg:journal.1045337", 
            "issn": [
              "2045-2322"
            ], 
            "name": "Scientific Reports", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "7"
          }
        ], 
        "keywords": [
          "terrestrial analog sites", 
          "pore water chemistry", 
          "microbial dark matter", 
          "tree of life", 
          "rRNA gene amplicon sequencing", 
          "diversity of life", 
          "gene amplicon sequencing", 
          "geochemical conditions", 
          "early Earth", 
          "analogue site", 
          "water chemistry", 
          "biogeochemical functions", 
          "prokaryotic lineages", 
          "carbon cycling", 
          "phylogenetic branching", 
          "sapropels", 
          "life habitat", 
          "polyphasic approach", 
          "candidate divisions", 
          "prokaryotic communities", 
          "novel ecosystems", 
          "amplicon sequencing", 
          "low water activity", 
          "RT-qPCR", 
          "celestial bodies", 
          "moderate occurrence", 
          "prokaryotes", 
          "phyla", 
          "Chloroflexi", 
          "habitats", 
          "Earth", 
          "taxa", 
          "Proteobacteria", 
          "lineages", 
          "proxy", 
          "Bacteroidetes", 
          "sequencing", 
          "ecosystems", 
          "bioinformatics", 
          "diversity", 
          "cycling", 
          "chemistry", 
          "hotspots", 
          "trees", 
          "water activity", 
          "occurrence", 
          "matter", 
          "division", 
          "branching", 
          "sites", 
          "role", 
          "border", 
          "characterization", 
          "activity", 
          "community", 
          "milieu", 
          "physicochemical investigations", 
          "date", 
          "function", 
          "conditions", 
          "body", 
          "investigation", 
          "relation", 
          "life", 
          "physicochemical characterization", 
          "approach", 
          "dark matter", 
          "Present-day terrestrial analogue sites", 
          "crucial ground truth proxies", 
          "ground truth proxies", 
          "truth proxies", 
          "hypersaline sapropels", 
          "disparate hypersaline sapropels", 
          "taxon-specific biogeochemical functions", 
          "deep coverage SSU rRNA gene amplicon sequencing", 
          "coverage SSU rRNA gene amplicon sequencing", 
          "SSU rRNA gene amplicon sequencing", 
          "analogous elemental milieu", 
          "elemental milieu", 
          "harbored prokaryotes", 
          "undocumented prokaryotic lineages"
        ], 
        "name": "Hypersaline sapropels act as hotspots for microbial dark matter", 
        "pagination": "6150", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1090754905"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/s41598-017-06232-w"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "28733590"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/s41598-017-06232-w", 
          "https://app.dimensions.ai/details/publication/pub.1090754905"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2021-11-01T18:28", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20211101/entities/gbq_results/article/article_735.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1038/s41598-017-06232-w"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-017-06232-w'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-017-06232-w'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-017-06232-w'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-017-06232-w'


     

    This table displays all metadata directly associated to this object as RDF triples.

    362 TRIPLES      22 PREDICATES      137 URIs      112 LITERALS      20 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/s41598-017-06232-w schema:about N3f5176701eba4df99c8350d3c4c12ae9
    2 N417dbc9a5f974ed5ae644129ca8e231a
    3 N466c866e51f14f2489b4fdef1fe63c33
    4 N4ca6a22df5d64323895882f1e014cda0
    5 N9949247b7df849debf694fa7c523f6d6
    6 N9aac1db9eb544678885f59d519c5df68
    7 Nacaec7cb987243c1a558d3f47121b17f
    8 Nd695b5b3d6d04ec9b63514260f7aab6b
    9 Nde8342ec3f2742bf89cf78d8f4761cad
    10 Ne26566a103ae40c1aa2cec893b6327af
    11 Ne46b15d773dd46ffbb34e6f6a91a919d
    12 Nf258fce683c440289fb309949dbfceea
    13 Nffcf176e811f446ead19b625ffd74d18
    14 anzsrc-for:04
    15 anzsrc-for:0402
    16 schema:author Nfe4f57ed50e7460cac0a5bb19e567091
    17 schema:citation sg:pub.10.1007/bf02905908
    18 sg:pub.10.1007/s00248-002-1061-9
    19 sg:pub.10.1007/s00248-008-9482-8
    20 sg:pub.10.1007/s00792-015-0738-1
    21 sg:pub.10.1007/s10482-014-0238-9
    22 sg:pub.10.1038/ismej.2010.3
    23 sg:pub.10.1038/ismej.2011.139
    24 sg:pub.10.1038/ismej.2011.82
    25 sg:pub.10.1038/ismej.2012.47
    26 sg:pub.10.1038/ismej.2013.152
    27 sg:pub.10.1038/ismej.2015.60
    28 sg:pub.10.1038/nature10855
    29 sg:pub.10.1038/ngeo2412
    30 sg:pub.10.1038/ngeo441
    31 sg:pub.10.1038/nmeth.2604
    32 sg:pub.10.1038/nmeth.2634
    33 sg:pub.10.1038/nmeth.f.303
    34 schema:datePublished 2017-07-21
    35 schema:datePublishedReg 2017-07-21
    36 schema:description Present-day terrestrial analogue sites are crucial ground truth proxies for studying life in geochemical conditions close to those assumed to be present on early Earth or inferred to exist on other celestial bodies (e.g. Mars, Europa). Although hypersaline sapropels are border-of-life habitats with moderate occurrence, their microbiological and physicochemical characterization lags behind. Here, we study the diversity of life under low water activity by describing the prokaryotic communities from two disparate hypersaline sapropels (Transylvanian Basin, Romania) in relation to geochemical milieu and pore water chemistry, while inferring their role in carbon cycling by matching taxa to known taxon-specific biogeochemical functions. The polyphasic approach combined deep coverage SSU rRNA gene amplicon sequencing and bioinformatics with RT-qPCR and physicochemical investigations. We found that sapropels developed an analogous elemental milieu and harbored prokaryotes affiliated with fifty-nine phyla, among which the most abundant were Proteobacteria, Bacteroidetes and Chloroflexi. Containing thirty-two candidate divisions and possibly undocumented prokaryotic lineages, the hypersaline sapropels were found to accommodate one of the most diverse and novel ecosystems reported to date and may contribute to completing the phylogenetic branching of the tree of life.
    37 schema:genre article
    38 schema:inLanguage en
    39 schema:isAccessibleForFree true
    40 schema:isPartOf N326012073d7a43dda74f4609674b4adf
    41 N7a722e84bea84477baba527bcfe4679c
    42 sg:journal.1045337
    43 schema:keywords Bacteroidetes
    44 Chloroflexi
    45 Earth
    46 Present-day terrestrial analogue sites
    47 Proteobacteria
    48 RT-qPCR
    49 SSU rRNA gene amplicon sequencing
    50 activity
    51 amplicon sequencing
    52 analogous elemental milieu
    53 analogue site
    54 approach
    55 biogeochemical functions
    56 bioinformatics
    57 body
    58 border
    59 branching
    60 candidate divisions
    61 carbon cycling
    62 celestial bodies
    63 characterization
    64 chemistry
    65 community
    66 conditions
    67 coverage SSU rRNA gene amplicon sequencing
    68 crucial ground truth proxies
    69 cycling
    70 dark matter
    71 date
    72 deep coverage SSU rRNA gene amplicon sequencing
    73 disparate hypersaline sapropels
    74 diversity
    75 diversity of life
    76 division
    77 early Earth
    78 ecosystems
    79 elemental milieu
    80 function
    81 gene amplicon sequencing
    82 geochemical conditions
    83 ground truth proxies
    84 habitats
    85 harbored prokaryotes
    86 hotspots
    87 hypersaline sapropels
    88 investigation
    89 life
    90 life habitat
    91 lineages
    92 low water activity
    93 matter
    94 microbial dark matter
    95 milieu
    96 moderate occurrence
    97 novel ecosystems
    98 occurrence
    99 phyla
    100 phylogenetic branching
    101 physicochemical characterization
    102 physicochemical investigations
    103 polyphasic approach
    104 pore water chemistry
    105 prokaryotes
    106 prokaryotic communities
    107 prokaryotic lineages
    108 proxy
    109 rRNA gene amplicon sequencing
    110 relation
    111 role
    112 sapropels
    113 sequencing
    114 sites
    115 taxa
    116 taxon-specific biogeochemical functions
    117 terrestrial analog sites
    118 tree of life
    119 trees
    120 truth proxies
    121 undocumented prokaryotic lineages
    122 water activity
    123 water chemistry
    124 schema:name Hypersaline sapropels act as hotspots for microbial dark matter
    125 schema:pagination 6150
    126 schema:productId Nceff6cb6455a4209b516cc48811c1d7d
    127 Ne842d4bfd69c4e17af03835610231a83
    128 Nfd9fb83df7a64c9fa7141c382934991e
    129 schema:sameAs https://app.dimensions.ai/details/publication/pub.1090754905
    130 https://doi.org/10.1038/s41598-017-06232-w
    131 schema:sdDatePublished 2021-11-01T18:28
    132 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    133 schema:sdPublisher Ne0193e4a11b6479d99969ac556de6474
    134 schema:url https://doi.org/10.1038/s41598-017-06232-w
    135 sgo:license sg:explorer/license/
    136 sgo:sdDataset articles
    137 rdf:type schema:ScholarlyArticle
    138 N20d359d3826942108a76e8a7efa4f0ce rdf:first sg:person.01170717014.39
    139 rdf:rest rdf:nil
    140 N326012073d7a43dda74f4609674b4adf schema:volumeNumber 7
    141 rdf:type schema:PublicationVolume
    142 N360b4ab197e64c64ac743429b4c8d715 rdf:first sg:person.014656472653.37
    143 rdf:rest Ne646b7c779374af39d1da526d28831e3
    144 N3f5176701eba4df99c8350d3c4c12ae9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    145 schema:name DNA, Ribosomal
    146 rdf:type schema:DefinedTerm
    147 N417dbc9a5f974ed5ae644129ca8e231a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    148 schema:name Lakes
    149 rdf:type schema:DefinedTerm
    150 N466c866e51f14f2489b4fdef1fe63c33 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    151 schema:name Archaea
    152 rdf:type schema:DefinedTerm
    153 N4ca6a22df5d64323895882f1e014cda0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    154 schema:name Humic Substances
    155 rdf:type schema:DefinedTerm
    156 N4d4bfe52edbc4aeca6c98c6775b46c27 rdf:first sg:person.01153051752.31
    157 rdf:rest Nfa5693cf30a540a2bdb4da6eb57677b2
    158 N50f15db76bb64f4895e2a2a5cb3c6782 rdf:first sg:person.01037500277.10
    159 rdf:rest N9171a4ea1f4c4ca5b2c4665265d6b962
    160 N7a722e84bea84477baba527bcfe4679c schema:issueNumber 1
    161 rdf:type schema:PublicationIssue
    162 N8203719d136d4ad6bcb32530c2055ce6 rdf:first sg:person.0660122652.37
    163 rdf:rest N360b4ab197e64c64ac743429b4c8d715
    164 N8dcb9a25539643d4b4e66f413a5e03f8 rdf:first sg:person.01334660037.45
    165 rdf:rest N20d359d3826942108a76e8a7efa4f0ce
    166 N9171a4ea1f4c4ca5b2c4665265d6b962 rdf:first sg:person.0614207202.10
    167 rdf:rest N8dcb9a25539643d4b4e66f413a5e03f8
    168 N92a177b059c04368b748ccde54a0e1ed rdf:first sg:person.01165653363.64
    169 rdf:rest N50f15db76bb64f4895e2a2a5cb3c6782
    170 N9949247b7df849debf694fa7c523f6d6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    171 schema:name Proteobacteria
    172 rdf:type schema:DefinedTerm
    173 N9aac1db9eb544678885f59d519c5df68 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    174 schema:name Geologic Sediments
    175 rdf:type schema:DefinedTerm
    176 Nacaec7cb987243c1a558d3f47121b17f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    177 schema:name DNA, Bacterial
    178 rdf:type schema:DefinedTerm
    179 Nc22a604cded74ffe9781887bc8a9c4a1 rdf:first sg:person.01151242114.36
    180 rdf:rest N92a177b059c04368b748ccde54a0e1ed
    181 Nceff6cb6455a4209b516cc48811c1d7d schema:name dimensions_id
    182 schema:value pub.1090754905
    183 rdf:type schema:PropertyValue
    184 Nd695b5b3d6d04ec9b63514260f7aab6b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    185 schema:name Benzopyrans
    186 rdf:type schema:DefinedTerm
    187 Nde8342ec3f2742bf89cf78d8f4761cad schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    188 schema:name Sequence Analysis, DNA
    189 rdf:type schema:DefinedTerm
    190 Ne0193e4a11b6479d99969ac556de6474 schema:name Springer Nature - SN SciGraph project
    191 rdf:type schema:Organization
    192 Ne26566a103ae40c1aa2cec893b6327af schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    193 schema:name Phylogeny
    194 rdf:type schema:DefinedTerm
    195 Ne46b15d773dd46ffbb34e6f6a91a919d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    196 schema:name Chloroflexi
    197 rdf:type schema:DefinedTerm
    198 Ne646b7c779374af39d1da526d28831e3 rdf:first sg:person.01113764741.65
    199 rdf:rest Nc22a604cded74ffe9781887bc8a9c4a1
    200 Ne842d4bfd69c4e17af03835610231a83 schema:name pubmed_id
    201 schema:value 28733590
    202 rdf:type schema:PropertyValue
    203 Nf258fce683c440289fb309949dbfceea schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    204 schema:name Romania
    205 rdf:type schema:DefinedTerm
    206 Nfa5693cf30a540a2bdb4da6eb57677b2 rdf:first sg:person.01040621745.31
    207 rdf:rest N8203719d136d4ad6bcb32530c2055ce6
    208 Nfd9fb83df7a64c9fa7141c382934991e schema:name doi
    209 schema:value 10.1038/s41598-017-06232-w
    210 rdf:type schema:PropertyValue
    211 Nfe4f57ed50e7460cac0a5bb19e567091 rdf:first sg:person.01003205261.19
    212 rdf:rest N4d4bfe52edbc4aeca6c98c6775b46c27
    213 Nffcf176e811f446ead19b625ffd74d18 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    214 schema:name Bacteroidetes
    215 rdf:type schema:DefinedTerm
    216 anzsrc-for:04 schema:inDefinedTermSet anzsrc-for:
    217 schema:name Earth Sciences
    218 rdf:type schema:DefinedTerm
    219 anzsrc-for:0402 schema:inDefinedTermSet anzsrc-for:
    220 schema:name Geochemistry
    221 rdf:type schema:DefinedTerm
    222 sg:journal.1045337 schema:issn 2045-2322
    223 schema:name Scientific Reports
    224 schema:publisher Springer Nature
    225 rdf:type schema:Periodical
    226 sg:person.01003205261.19 schema:affiliation grid-institutes:grid.448010.9
    227 schema:familyName Andrei
    228 schema:givenName Adrian-Ştefan
    229 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01003205261.19
    230 rdf:type schema:Person
    231 sg:person.01037500277.10 schema:affiliation grid-institutes:grid.483232.c
    232 schema:familyName Levei
    233 schema:givenName Erika Andrea
    234 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01037500277.10
    235 rdf:type schema:Person
    236 sg:person.01040621745.31 schema:affiliation grid-institutes:None
    237 schema:familyName Robeson
    238 schema:givenName Michael Scott
    239 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01040621745.31
    240 rdf:type schema:Person
    241 sg:person.01113764741.65 schema:affiliation grid-institutes:grid.435400.6
    242 schema:familyName Chiriac
    243 schema:givenName Cecilia
    244 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01113764741.65
    245 rdf:type schema:Person
    246 sg:person.01151242114.36 schema:affiliation grid-institutes:grid.435400.6
    247 schema:familyName Szekeres
    248 schema:givenName Edina
    249 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01151242114.36
    250 rdf:type schema:Person
    251 sg:person.01153051752.31 schema:affiliation grid-institutes:grid.435400.6
    252 schema:familyName Baricz
    253 schema:givenName Andreea
    254 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01153051752.31
    255 rdf:type schema:Person
    256 sg:person.01165653363.64 schema:affiliation grid-institutes:grid.7399.4
    257 schema:familyName Barbu-Tudoran
    258 schema:givenName Lucian
    259 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01165653363.64
    260 rdf:type schema:Person
    261 sg:person.01170717014.39 schema:affiliation grid-institutes:grid.7399.4
    262 schema:familyName Banciu
    263 schema:givenName Horia Leonard
    264 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01170717014.39
    265 rdf:type schema:Person
    266 sg:person.01334660037.45 schema:affiliation grid-institutes:grid.135519.a
    267 schema:familyName Podar
    268 schema:givenName Mircea
    269 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01334660037.45
    270 rdf:type schema:Person
    271 sg:person.014656472653.37 schema:affiliation grid-institutes:grid.7399.4
    272 schema:familyName Tămaş
    273 schema:givenName Tudor
    274 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014656472653.37
    275 rdf:type schema:Person
    276 sg:person.0614207202.10 schema:affiliation grid-institutes:grid.435400.6
    277 schema:familyName Coman
    278 schema:givenName Cristian
    279 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0614207202.10
    280 rdf:type schema:Person
    281 sg:person.0660122652.37 schema:affiliation grid-institutes:grid.11598.34
    282 schema:familyName Păuşan
    283 schema:givenName Manuela Raluca
    284 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0660122652.37
    285 rdf:type schema:Person
    286 sg:pub.10.1007/bf02905908 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017837825
    287 https://doi.org/10.1007/bf02905908
    288 rdf:type schema:CreativeWork
    289 sg:pub.10.1007/s00248-002-1061-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034162851
    290 https://doi.org/10.1007/s00248-002-1061-9
    291 rdf:type schema:CreativeWork
    292 sg:pub.10.1007/s00248-008-9482-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038922548
    293 https://doi.org/10.1007/s00248-008-9482-8
    294 rdf:type schema:CreativeWork
    295 sg:pub.10.1007/s00792-015-0738-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051387523
    296 https://doi.org/10.1007/s00792-015-0738-1
    297 rdf:type schema:CreativeWork
    298 sg:pub.10.1007/s10482-014-0238-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006165818
    299 https://doi.org/10.1007/s10482-014-0238-9
    300 rdf:type schema:CreativeWork
    301 sg:pub.10.1038/ismej.2010.3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019352157
    302 https://doi.org/10.1038/ismej.2010.3
    303 rdf:type schema:CreativeWork
    304 sg:pub.10.1038/ismej.2011.139 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051863807
    305 https://doi.org/10.1038/ismej.2011.139
    306 rdf:type schema:CreativeWork
    307 sg:pub.10.1038/ismej.2011.82 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033211760
    308 https://doi.org/10.1038/ismej.2011.82
    309 rdf:type schema:CreativeWork
    310 sg:pub.10.1038/ismej.2012.47 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044196707
    311 https://doi.org/10.1038/ismej.2012.47
    312 rdf:type schema:CreativeWork
    313 sg:pub.10.1038/ismej.2013.152 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035056123
    314 https://doi.org/10.1038/ismej.2013.152
    315 rdf:type schema:CreativeWork
    316 sg:pub.10.1038/ismej.2015.60 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015256313
    317 https://doi.org/10.1038/ismej.2015.60
    318 rdf:type schema:CreativeWork
    319 sg:pub.10.1038/nature10855 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032561330
    320 https://doi.org/10.1038/nature10855
    321 rdf:type schema:CreativeWork
    322 sg:pub.10.1038/ngeo2412 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029203295
    323 https://doi.org/10.1038/ngeo2412
    324 rdf:type schema:CreativeWork
    325 sg:pub.10.1038/ngeo441 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039147523
    326 https://doi.org/10.1038/ngeo441
    327 rdf:type schema:CreativeWork
    328 sg:pub.10.1038/nmeth.2604 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027743851
    329 https://doi.org/10.1038/nmeth.2604
    330 rdf:type schema:CreativeWork
    331 sg:pub.10.1038/nmeth.2634 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039971505
    332 https://doi.org/10.1038/nmeth.2634
    333 rdf:type schema:CreativeWork
    334 sg:pub.10.1038/nmeth.f.303 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009032055
    335 https://doi.org/10.1038/nmeth.f.303
    336 rdf:type schema:CreativeWork
    337 grid-institutes:None schema:alternateName Interstitial Genomics, LLC, Longmont, 80501 Colorado USA
    338 schema:name Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee USA
    339 Interstitial Genomics, LLC, Longmont, 80501 Colorado USA
    340 rdf:type schema:Organization
    341 grid-institutes:grid.11598.34 schema:alternateName Department for Internal Medicine, Medical University of Graz, Graz, Austria
    342 schema:name Department for Internal Medicine, Medical University of Graz, Graz, Austria
    343 rdf:type schema:Organization
    344 grid-institutes:grid.135519.a schema:alternateName Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee USA
    345 schema:name Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee USA
    346 rdf:type schema:Organization
    347 grid-institutes:grid.435400.6 schema:alternateName National Institute of Research and Development for Biological Sciences (NIRDBS), Institute of Biological Research, Cluj-Napoca, Romania
    348 schema:name Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babeş-Bolyai University, Cluj-Napoca, Romania
    349 National Institute of Research and Development for Biological Sciences (NIRDBS), Institute of Biological Research, Cluj-Napoca, Romania
    350 rdf:type schema:Organization
    351 grid-institutes:grid.448010.9 schema:alternateName Institute of Hydrobiology, Department of Aquatic Microbial Ecology, Biology Center of the Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic
    352 schema:name Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babeş-Bolyai University, Cluj-Napoca, Romania
    353 Institute of Hydrobiology, Department of Aquatic Microbial Ecology, Biology Center of the Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic
    354 rdf:type schema:Organization
    355 grid-institutes:grid.483232.c schema:alternateName INCDO-INOE 2000, Research Institute for Analytical Instrumentation, Cluj-Napoca, Romania
    356 schema:name INCDO-INOE 2000, Research Institute for Analytical Instrumentation, Cluj-Napoca, Romania
    357 rdf:type schema:Organization
    358 grid-institutes:grid.7399.4 schema:alternateName Department of Geology, Faculty of Biology and Geology, Babeş-Bolyai University, Cluj-Napoca, Romania
    359 Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babeş-Bolyai University, Cluj-Napoca, Romania
    360 schema:name Department of Geology, Faculty of Biology and Geology, Babeş-Bolyai University, Cluj-Napoca, Romania
    361 Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babeş-Bolyai University, Cluj-Napoca, Romania
    362 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...