The importance of N-glycosylation on β3 integrin ligand binding and conformational regulation View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-12

AUTHORS

Xiulei Cai, Aye Myat Myat Thinn, Zhengli Wang, Hu Shan, Jieqing Zhu

ABSTRACT

N-glycosylations can regulate the adhesive function of integrins. Great variations in both the number and distribution of N-glycosylation sites are found in the 18 α and 8 β integrin subunits. Crystal structures of αIIbβ3 and αVβ3 have resolved the precise structural location of each N-glycan site, but the structural consequences of individual N-glycan site on integrin activation remain unclear. By site-directed mutagenesis and structure-guided analyses, we dissected the function of individual N-glycan sites in β3 integrin activation. We found that the N-glycan site, β3-N320 at the headpiece and leg domain interface positively regulates αIIbβ3 but not αVβ3 activation. The β3-N559 N-glycan at the β3-I-EGF3 and αIIb-calf-1 domain interface, and the β3-N654 N-glycan at the β3-β-tail and αIIb-calf-2 domain interface positively regulate the activation of both αIIbβ3 and αVβ3 integrins. In contrast, removal of the β3-N371 N-glycan near the β3 hybrid and I-EGF3 interface, or the β3-N452 N-glycan at the I-EGF1 domain rendered β3 integrin more active than the wild type. We identified one unique N-glycan at the βI domain of β1 subunit that negatively regulates α5β1 activation. Our study suggests that the bulky N-glycans influence the large-scale conformational rearrangement by potentially stabilizing or destabilizing the domain interfaces of integrin. More... »

PAGES

4656

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-017-04844-w

DOI

http://dx.doi.org/10.1038/s41598-017-04844-w

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1090351943

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28680094


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Qingdao Agricultural University", 
          "id": "https://www.grid.ac/institutes/grid.412608.9", 
          "name": [
            "Blood Research Institute, BloodCenter of Wisconsin, 53226, Milwaukee, WI, USA", 
            "College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, 266109, Qingdao, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cai", 
        "givenName": "Xiulei", 
        "id": "sg:person.015601067533.64", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015601067533.64"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Medical College of Wisconsin", 
          "id": "https://www.grid.ac/institutes/grid.30760.32", 
          "name": [
            "Blood Research Institute, BloodCenter of Wisconsin, 53226, Milwaukee, WI, USA", 
            "Department of Biochemistry, Medical College of Wisconsin, 53226, Milwaukee, WI, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Thinn", 
        "givenName": "Aye Myat Myat", 
        "id": "sg:person.01220512435.79", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01220512435.79"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Bloodcenter of Wisconsin", 
          "id": "https://www.grid.ac/institutes/grid.280427.b", 
          "name": [
            "Blood Research Institute, BloodCenter of Wisconsin, 53226, Milwaukee, WI, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wang", 
        "givenName": "Zhengli", 
        "id": "sg:person.07673376733.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07673376733.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Qingdao Agricultural University", 
          "id": "https://www.grid.ac/institutes/grid.412608.9", 
          "name": [
            "College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, 266109, Qingdao, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shan", 
        "givenName": "Hu", 
        "id": "sg:person.0765222014.91", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0765222014.91"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Medical College of Wisconsin", 
          "id": "https://www.grid.ac/institutes/grid.30760.32", 
          "name": [
            "Blood Research Institute, BloodCenter of Wisconsin, 53226, Milwaukee, WI, USA", 
            "Department of Biochemistry, Medical College of Wisconsin, 53226, Milwaukee, WI, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhu", 
        "givenName": "Jieqing", 
        "id": "sg:person.010475616717.67", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010475616717.67"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/j.bbagen.2008.07.011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000874082"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/jcs.137828", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002762059"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m409548200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004193646"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/glycob/3.2.97", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007325615"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/jth.12915", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009289838"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1503262112", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011173411"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature10697", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011638788", 
          "https://doi.org/10.1038/nature10697"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(02)00971-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012945203"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.201212037", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013215261"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1248/bpb.32.780", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013493782"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m607771200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013723129"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/bs.acr.2014.11.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015147022"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tcb.2014.12.006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018995600"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm2871", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020514176", 
          "https://doi.org/10.1038/nrm2871"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm2871", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020514176", 
          "https://doi.org/10.1038/nrm2871"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.5483/bmbrep.2011.44.12.772", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021227613"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0438060100", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024778296"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2009.02.022", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024917927"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1096/fj.201600665r", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025451959"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbamcr.2013.04.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026147803"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/srep33507", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026824713", 
          "https://doi.org/10.1038/srep33507"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/jcs.160663", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027761460"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m807920200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028052944"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m709527200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028072259"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.201111077", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028886423"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ceb.2011.10.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030482205"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m700249200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030532312"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1600-065x.2009.00795.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030597936"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1600-065x.2009.00795.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030597936"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm3383", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030860102", 
          "https://doi.org/10.1038/nrm3383"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/blood-2008-06-077891", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031500433"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m110.139667", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032227291"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbagen.2016.08.007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032246125"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature02976", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032545396", 
          "https://doi.org/10.1038/nature02976"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature02976", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032545396", 
          "https://doi.org/10.1038/nature02976"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci64100", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032622360"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbagen.2010.03.013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033104296"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1349-7006.2008.00839.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033152329"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/prot.22126", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033600897"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.cellbio.21.090704.151217", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033852527"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.yexcr.2014.02.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037077825"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.200905085", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037348986"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2008.11.018", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038611339"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ni.f.203", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039419058", 
          "https://doi.org/10.1038/ni.f.203"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.274.40.28071", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042886020"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m111.311043", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044492297"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci110973", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047131496"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1742-4658.2008.06346.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047411587"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.270.21.12411", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047897770"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/blood-2005-07-2990", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050884851"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.mam.2016.04.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051338123"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m807660200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053320254"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/glycob/cwt014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053515012"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi300734n", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055205242"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bst20140206", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056717761"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bst20140206", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056717761"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m116.756502", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1058215662"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1064535", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062445423"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1086652", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062448313"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.15252/embj.201695803", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074203823"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075198229", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077345066", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078798557", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079070236", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.00558-16", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083687571"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2017-12", 
    "datePublishedReg": "2017-12-01", 
    "description": "N-glycosylations can regulate the adhesive function of integrins. Great variations in both the number and distribution of N-glycosylation sites are found in the 18 \u03b1 and 8 \u03b2 integrin subunits. Crystal structures of \u03b1IIb\u03b23 and \u03b1V\u03b23 have resolved the precise structural location of each N-glycan site, but the structural consequences of individual N-glycan site on integrin activation remain unclear. By site-directed mutagenesis and structure-guided analyses, we dissected the function of individual N-glycan sites in \u03b23 integrin activation. We found that the N-glycan site, \u03b23-N320 at the headpiece and leg domain interface positively regulates \u03b1IIb\u03b23 but not \u03b1V\u03b23 activation. The \u03b23-N559 N-glycan at the \u03b23-I-EGF3 and \u03b1IIb-calf-1 domain interface, and the \u03b23-N654 N-glycan at the \u03b23-\u03b2-tail and \u03b1IIb-calf-2 domain interface positively regulate the activation of both \u03b1IIb\u03b23 and \u03b1V\u03b23 integrins. In contrast, removal of the \u03b23-N371 N-glycan near the \u03b23 hybrid and I-EGF3 interface, or the \u03b23-N452 N-glycan at the I-EGF1 domain rendered \u03b23 integrin more active than the wild type. We identified one unique N-glycan at the \u03b2I domain of \u03b21 subunit that negatively regulates \u03b15\u03b21 activation. Our study suggests that the bulky N-glycans influence the large-scale conformational rearrangement by potentially stabilizing or destabilizing the domain interfaces of integrin.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/s41598-017-04844-w", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.6377566", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.4244632", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1045337", 
        "issn": [
          "2045-2322"
        ], 
        "name": "Scientific Reports", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "7"
      }
    ], 
    "name": "The importance of N-glycosylation on \u03b23 integrin ligand binding and conformational regulation", 
    "pagination": "4656", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "e3095a12595b12b642a7681af68115c35c4d152fd475e3fb92359c0f750abb47"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "28680094"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101563288"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/s41598-017-04844-w"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1090351943"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/s41598-017-04844-w", 
      "https://app.dimensions.ai/details/publication/pub.1090351943"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T23:20", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8693_00000493.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/s41598-017-04844-w"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-017-04844-w'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-017-04844-w'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-017-04844-w'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-017-04844-w'


 

This table displays all metadata directly associated to this object as RDF triples.

293 TRIPLES      21 PREDICATES      90 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/s41598-017-04844-w schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N8bbb163686fd48dfa455cba3d93927e5
4 schema:citation sg:pub.10.1038/nature02976
5 sg:pub.10.1038/nature10697
6 sg:pub.10.1038/ni.f.203
7 sg:pub.10.1038/nrm2871
8 sg:pub.10.1038/nrm3383
9 sg:pub.10.1038/srep33507
10 https://app.dimensions.ai/details/publication/pub.1075198229
11 https://app.dimensions.ai/details/publication/pub.1077345066
12 https://app.dimensions.ai/details/publication/pub.1078798557
13 https://app.dimensions.ai/details/publication/pub.1079070236
14 https://doi.org/10.1002/prot.22126
15 https://doi.org/10.1016/bs.acr.2014.11.001
16 https://doi.org/10.1016/j.bbagen.2008.07.011
17 https://doi.org/10.1016/j.bbagen.2010.03.013
18 https://doi.org/10.1016/j.bbagen.2016.08.007
19 https://doi.org/10.1016/j.bbamcr.2013.04.001
20 https://doi.org/10.1016/j.ceb.2011.10.004
21 https://doi.org/10.1016/j.mam.2016.04.008
22 https://doi.org/10.1016/j.molcel.2008.11.018
23 https://doi.org/10.1016/j.molcel.2009.02.022
24 https://doi.org/10.1016/j.tcb.2014.12.006
25 https://doi.org/10.1016/j.yexcr.2014.02.004
26 https://doi.org/10.1016/s0092-8674(02)00971-6
27 https://doi.org/10.1021/bi300734n
28 https://doi.org/10.1042/bst20140206
29 https://doi.org/10.1073/pnas.0438060100
30 https://doi.org/10.1073/pnas.1503262112
31 https://doi.org/10.1074/jbc.270.21.12411
32 https://doi.org/10.1074/jbc.274.40.28071
33 https://doi.org/10.1074/jbc.m110.139667
34 https://doi.org/10.1074/jbc.m111.311043
35 https://doi.org/10.1074/jbc.m116.756502
36 https://doi.org/10.1074/jbc.m409548200
37 https://doi.org/10.1074/jbc.m607771200
38 https://doi.org/10.1074/jbc.m700249200
39 https://doi.org/10.1074/jbc.m709527200
40 https://doi.org/10.1074/jbc.m807660200
41 https://doi.org/10.1074/jbc.m807920200
42 https://doi.org/10.1083/jcb.200905085
43 https://doi.org/10.1083/jcb.201111077
44 https://doi.org/10.1083/jcb.201212037
45 https://doi.org/10.1093/glycob/3.2.97
46 https://doi.org/10.1093/glycob/cwt014
47 https://doi.org/10.1096/fj.201600665r
48 https://doi.org/10.1111/j.1349-7006.2008.00839.x
49 https://doi.org/10.1111/j.1600-065x.2009.00795.x
50 https://doi.org/10.1111/j.1742-4658.2008.06346.x
51 https://doi.org/10.1111/jth.12915
52 https://doi.org/10.1126/science.1064535
53 https://doi.org/10.1126/science.1086652
54 https://doi.org/10.1128/mcb.00558-16
55 https://doi.org/10.1146/annurev.cellbio.21.090704.151217
56 https://doi.org/10.1172/jci110973
57 https://doi.org/10.1172/jci64100
58 https://doi.org/10.1182/blood-2005-07-2990
59 https://doi.org/10.1182/blood-2008-06-077891
60 https://doi.org/10.1242/jcs.137828
61 https://doi.org/10.1242/jcs.160663
62 https://doi.org/10.1248/bpb.32.780
63 https://doi.org/10.15252/embj.201695803
64 https://doi.org/10.5483/bmbrep.2011.44.12.772
65 schema:datePublished 2017-12
66 schema:datePublishedReg 2017-12-01
67 schema:description N-glycosylations can regulate the adhesive function of integrins. Great variations in both the number and distribution of N-glycosylation sites are found in the 18 α and 8 β integrin subunits. Crystal structures of α<sub>IIb</sub>β<sub>3</sub> and α<sub>V</sub>β<sub>3</sub> have resolved the precise structural location of each N-glycan site, but the structural consequences of individual N-glycan site on integrin activation remain unclear. By site-directed mutagenesis and structure-guided analyses, we dissected the function of individual N-glycan sites in β<sub>3</sub> integrin activation. We found that the N-glycan site, β<sub>3</sub>-N320 at the headpiece and leg domain interface positively regulates α<sub>IIb</sub>β<sub>3</sub> but not α<sub>V</sub>β<sub>3</sub> activation. The β<sub>3</sub>-N559 N-glycan at the β<sub>3</sub>-I-EGF3 and α<sub>IIb</sub>-calf-1 domain interface, and the β<sub>3</sub>-N654 N-glycan at the β<sub>3</sub>-β-tail and α<sub>IIb</sub>-calf-2 domain interface positively regulate the activation of both α<sub>IIb</sub>β<sub>3</sub> and α<sub>V</sub>β<sub>3</sub> integrins. In contrast, removal of the β<sub>3</sub>-N371 N-glycan near the β<sub>3</sub> hybrid and I-EGF3 interface, or the β<sub>3</sub>-N452 N-glycan at the I-EGF1 domain rendered β<sub>3</sub> integrin more active than the wild type. We identified one unique N-glycan at the βI domain of β<sub>1</sub> subunit that negatively regulates α<sub>5</sub>β<sub>1</sub> activation. Our study suggests that the bulky N-glycans influence the large-scale conformational rearrangement by potentially stabilizing or destabilizing the domain interfaces of integrin.
68 schema:genre research_article
69 schema:inLanguage en
70 schema:isAccessibleForFree true
71 schema:isPartOf N71f6ca93688e409982164a8570412565
72 Nb226d9fa9aaf4250ac1108ff499747a0
73 sg:journal.1045337
74 schema:name The importance of N-glycosylation on β3 integrin ligand binding and conformational regulation
75 schema:pagination 4656
76 schema:productId N12f3ffcc32a44a16b8872634f8d853e9
77 N1c3e76b330ca44fd8f3995784c9f9da3
78 N2c14679b36bd4967a0e5531bea91a998
79 N8c35021dcdb442e69dcf0df24b46e920
80 Nfbedb81380bb4e10ab29c5ab437ad962
81 schema:sameAs https://app.dimensions.ai/details/publication/pub.1090351943
82 https://doi.org/10.1038/s41598-017-04844-w
83 schema:sdDatePublished 2019-04-10T23:20
84 schema:sdLicense https://scigraph.springernature.com/explorer/license/
85 schema:sdPublisher Nd260a0064f0f4fd9a2f83acc54c20f9e
86 schema:url https://www.nature.com/articles/s41598-017-04844-w
87 sgo:license sg:explorer/license/
88 sgo:sdDataset articles
89 rdf:type schema:ScholarlyArticle
90 N12f3ffcc32a44a16b8872634f8d853e9 schema:name doi
91 schema:value 10.1038/s41598-017-04844-w
92 rdf:type schema:PropertyValue
93 N192b6299d7184568a5abc40728029248 rdf:first sg:person.01220512435.79
94 rdf:rest N2349c33a767640168bedeacb05421b04
95 N1c3e76b330ca44fd8f3995784c9f9da3 schema:name dimensions_id
96 schema:value pub.1090351943
97 rdf:type schema:PropertyValue
98 N2349c33a767640168bedeacb05421b04 rdf:first sg:person.07673376733.06
99 rdf:rest Nd3f40a18eb2c40e397d4534dbc5e4b3f
100 N2c14679b36bd4967a0e5531bea91a998 schema:name nlm_unique_id
101 schema:value 101563288
102 rdf:type schema:PropertyValue
103 N71f6ca93688e409982164a8570412565 schema:volumeNumber 7
104 rdf:type schema:PublicationVolume
105 N8bbb163686fd48dfa455cba3d93927e5 rdf:first sg:person.015601067533.64
106 rdf:rest N192b6299d7184568a5abc40728029248
107 N8c35021dcdb442e69dcf0df24b46e920 schema:name readcube_id
108 schema:value e3095a12595b12b642a7681af68115c35c4d152fd475e3fb92359c0f750abb47
109 rdf:type schema:PropertyValue
110 Nb226d9fa9aaf4250ac1108ff499747a0 schema:issueNumber 1
111 rdf:type schema:PublicationIssue
112 Nb237fd5ba3ea4fada075fcc078b4cd56 rdf:first sg:person.010475616717.67
113 rdf:rest rdf:nil
114 Nd260a0064f0f4fd9a2f83acc54c20f9e schema:name Springer Nature - SN SciGraph project
115 rdf:type schema:Organization
116 Nd3f40a18eb2c40e397d4534dbc5e4b3f rdf:first sg:person.0765222014.91
117 rdf:rest Nb237fd5ba3ea4fada075fcc078b4cd56
118 Nfbedb81380bb4e10ab29c5ab437ad962 schema:name pubmed_id
119 schema:value 28680094
120 rdf:type schema:PropertyValue
121 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
122 schema:name Biological Sciences
123 rdf:type schema:DefinedTerm
124 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
125 schema:name Biochemistry and Cell Biology
126 rdf:type schema:DefinedTerm
127 sg:grant.4244632 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-017-04844-w
128 rdf:type schema:MonetaryGrant
129 sg:grant.6377566 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-017-04844-w
130 rdf:type schema:MonetaryGrant
131 sg:journal.1045337 schema:issn 2045-2322
132 schema:name Scientific Reports
133 rdf:type schema:Periodical
134 sg:person.010475616717.67 schema:affiliation https://www.grid.ac/institutes/grid.30760.32
135 schema:familyName Zhu
136 schema:givenName Jieqing
137 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010475616717.67
138 rdf:type schema:Person
139 sg:person.01220512435.79 schema:affiliation https://www.grid.ac/institutes/grid.30760.32
140 schema:familyName Thinn
141 schema:givenName Aye Myat Myat
142 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01220512435.79
143 rdf:type schema:Person
144 sg:person.015601067533.64 schema:affiliation https://www.grid.ac/institutes/grid.412608.9
145 schema:familyName Cai
146 schema:givenName Xiulei
147 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015601067533.64
148 rdf:type schema:Person
149 sg:person.0765222014.91 schema:affiliation https://www.grid.ac/institutes/grid.412608.9
150 schema:familyName Shan
151 schema:givenName Hu
152 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0765222014.91
153 rdf:type schema:Person
154 sg:person.07673376733.06 schema:affiliation https://www.grid.ac/institutes/grid.280427.b
155 schema:familyName Wang
156 schema:givenName Zhengli
157 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07673376733.06
158 rdf:type schema:Person
159 sg:pub.10.1038/nature02976 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032545396
160 https://doi.org/10.1038/nature02976
161 rdf:type schema:CreativeWork
162 sg:pub.10.1038/nature10697 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011638788
163 https://doi.org/10.1038/nature10697
164 rdf:type schema:CreativeWork
165 sg:pub.10.1038/ni.f.203 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039419058
166 https://doi.org/10.1038/ni.f.203
167 rdf:type schema:CreativeWork
168 sg:pub.10.1038/nrm2871 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020514176
169 https://doi.org/10.1038/nrm2871
170 rdf:type schema:CreativeWork
171 sg:pub.10.1038/nrm3383 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030860102
172 https://doi.org/10.1038/nrm3383
173 rdf:type schema:CreativeWork
174 sg:pub.10.1038/srep33507 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026824713
175 https://doi.org/10.1038/srep33507
176 rdf:type schema:CreativeWork
177 https://app.dimensions.ai/details/publication/pub.1075198229 schema:CreativeWork
178 https://app.dimensions.ai/details/publication/pub.1077345066 schema:CreativeWork
179 https://app.dimensions.ai/details/publication/pub.1078798557 schema:CreativeWork
180 https://app.dimensions.ai/details/publication/pub.1079070236 schema:CreativeWork
181 https://doi.org/10.1002/prot.22126 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033600897
182 rdf:type schema:CreativeWork
183 https://doi.org/10.1016/bs.acr.2014.11.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015147022
184 rdf:type schema:CreativeWork
185 https://doi.org/10.1016/j.bbagen.2008.07.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000874082
186 rdf:type schema:CreativeWork
187 https://doi.org/10.1016/j.bbagen.2010.03.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033104296
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1016/j.bbagen.2016.08.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032246125
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1016/j.bbamcr.2013.04.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026147803
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1016/j.ceb.2011.10.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030482205
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1016/j.mam.2016.04.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051338123
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1016/j.molcel.2008.11.018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038611339
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1016/j.molcel.2009.02.022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024917927
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1016/j.tcb.2014.12.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018995600
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1016/j.yexcr.2014.02.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037077825
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1016/s0092-8674(02)00971-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012945203
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1021/bi300734n schema:sameAs https://app.dimensions.ai/details/publication/pub.1055205242
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1042/bst20140206 schema:sameAs https://app.dimensions.ai/details/publication/pub.1056717761
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1073/pnas.0438060100 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024778296
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1073/pnas.1503262112 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011173411
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1074/jbc.270.21.12411 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047897770
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1074/jbc.274.40.28071 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042886020
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1074/jbc.m110.139667 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032227291
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1074/jbc.m111.311043 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044492297
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1074/jbc.m116.756502 schema:sameAs https://app.dimensions.ai/details/publication/pub.1058215662
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1074/jbc.m409548200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004193646
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1074/jbc.m607771200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013723129
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1074/jbc.m700249200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030532312
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1074/jbc.m709527200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028072259
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1074/jbc.m807660200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053320254
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1074/jbc.m807920200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028052944
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1083/jcb.200905085 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037348986
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1083/jcb.201111077 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028886423
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1083/jcb.201212037 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013215261
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1093/glycob/3.2.97 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007325615
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1093/glycob/cwt014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053515012
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1096/fj.201600665r schema:sameAs https://app.dimensions.ai/details/publication/pub.1025451959
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1111/j.1349-7006.2008.00839.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1033152329
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1111/j.1600-065x.2009.00795.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1030597936
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1111/j.1742-4658.2008.06346.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1047411587
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1111/jth.12915 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009289838
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1126/science.1064535 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062445423
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1126/science.1086652 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062448313
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1128/mcb.00558-16 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083687571
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1146/annurev.cellbio.21.090704.151217 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033852527
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1172/jci110973 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047131496
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1172/jci64100 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032622360
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1182/blood-2005-07-2990 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050884851
270 rdf:type schema:CreativeWork
271 https://doi.org/10.1182/blood-2008-06-077891 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031500433
272 rdf:type schema:CreativeWork
273 https://doi.org/10.1242/jcs.137828 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002762059
274 rdf:type schema:CreativeWork
275 https://doi.org/10.1242/jcs.160663 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027761460
276 rdf:type schema:CreativeWork
277 https://doi.org/10.1248/bpb.32.780 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013493782
278 rdf:type schema:CreativeWork
279 https://doi.org/10.15252/embj.201695803 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074203823
280 rdf:type schema:CreativeWork
281 https://doi.org/10.5483/bmbrep.2011.44.12.772 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021227613
282 rdf:type schema:CreativeWork
283 https://www.grid.ac/institutes/grid.280427.b schema:alternateName Bloodcenter of Wisconsin
284 schema:name Blood Research Institute, BloodCenter of Wisconsin, 53226, Milwaukee, WI, USA
285 rdf:type schema:Organization
286 https://www.grid.ac/institutes/grid.30760.32 schema:alternateName Medical College of Wisconsin
287 schema:name Blood Research Institute, BloodCenter of Wisconsin, 53226, Milwaukee, WI, USA
288 Department of Biochemistry, Medical College of Wisconsin, 53226, Milwaukee, WI, USA
289 rdf:type schema:Organization
290 https://www.grid.ac/institutes/grid.412608.9 schema:alternateName Qingdao Agricultural University
291 schema:name Blood Research Institute, BloodCenter of Wisconsin, 53226, Milwaukee, WI, USA
292 College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, 266109, Qingdao, China
293 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...