Eigen-analysis reveals components supporting super-resolution imaging of blinking fluorophores View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-12

AUTHORS

Krishna Agarwal, Dilip K. Prasad

ABSTRACT

This paper presents eigen-analysis of image stack of blinking fluorophores to identify the components that enable super-resolved imaging of blinking fluorophores. Eigen-analysis reveals that the contributions of spatial distribution of fluorophores and their temporal photon emission characteristics can be completely separated. While cross-emitter cross-pixel information of spatial distribution that permits super-resolution is encoded in two matrices, temporal statistics weigh the contribution of these matrices to the measured data. The properties and conditions of exploitation of these matrices are investigated. Con-temporary super-resolution imaging methods that use blinking for super-resolution are studied in the context of the presented analysis. Besides providing insight into the capabilities and limitations of existing super-resolution methods, the analysis shall help in designing better super-resolution techniques that directly exploit these matrices. More... »

PAGES

4445

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-017-04544-5

DOI

http://dx.doi.org/10.1038/s41598-017-04544-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1087305658

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28667336


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