Controlled functional expression of the bacteriocins pediocin PA-1 and bactofencin A in Escherichia coli View Full Text


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Article Info

DATE

2017-06-08

AUTHORS

Beatriz Mesa-Pereira, Paula M. O’Connor, Mary C. Rea, Paul D. Cotter, Colin Hill, R. Paul Ross

ABSTRACT

The bacteriocins bactofencin A (class IId) and pediocin PA-1 (class IIa) are encoded by operons with a similarly clustered gene organization including a structural peptide, an immunity protein, an ABC transporter and accessory bacteriocin transporter protein. Cloning of these operons in E. coli TunerTM (DE3) on a pETcoco-2 derived vector resulted in successful secretion of both bacteriocins. A corresponding approach, involving the construction of vectors containing different combinations of these genes, revealed that the structural and the transporter genes alone are sufficient to permit heterologous production and secretion in this host. Even though the accessory protein, usually associated with optimal disulfide bond formation, was not required for bacteriocin synthesis, its presence did result in greater pediocin PA-1 production. The simplicity of the system and the fact that the associated bacteriocins could be recovered from the extracellular medium provides an opportunity to facilitate protein engineering and the overproduction of biologically-active bacteriocins at industrial scale. Additionally, this system could enable the characterization of new bacteriocin operons where genetic tools are not available for the native producers. More... »

PAGES

3069

References to SciGraph publications

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  • 2013-06-22. Cloning, production, and functional expression of the bacteriocin sakacin A (SakA) and two SakA-derived chimeras in lactic acid bacteria (LAB) and the yeasts Pichia pastoris and Kluyveromyces lactis in JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
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  • 2007-06-27. Cloning, production and expression of the bacteriocin enterocin A produced by Enterococcus faecium PLBC21 in Lactococcus lactis in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2012-12-24. Bacteriocins — a viable alternative to antibiotics? in NATURE REVIEWS MICROBIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41598-017-02868-w

    DOI

    http://dx.doi.org/10.1038/s41598-017-02868-w

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1085846235

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/28596555


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    78 operon
    79 opportunities
    80 optimal disulfide bond formation
    81 organization
    82 overproduction
    83 pediocin PA-1
    84 pediocin PA-1 production
    85 peptides
    86 presence
    87 producers
    88 production
    89 protein
    90 protein engineering
    91 scale
    92 secretion
    93 simplicity
    94 structural peptides
    95 successful secretion
    96 sup
    97 synthesis
    98 system
    99 tool
    100 transporter gene
    101 transporter proteins
    102 transporters
    103 tuner
    104 vector
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