Enhancing the genome editing toolbox: genome wide CRISPR arrayed libraries View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-12

AUTHORS

Emmanouil Metzakopian, Alex Strong, Vivek Iyer, Alex Hodgkins, Konstantinos Tzelepis, Liliana Antunes, Mathias J Friedrich, Qiaohua Kang, Teresa Davidson, Jacob Lamberth, Christina Hoffmann, Gregory D. Davis, George S. Vassiliou, William C. Skarnes, Allan Bradley

ABSTRACT

CRISPR-Cas9 technology has accelerated biological research becoming routine for many laboratories. It is rapidly replacing conventional gene editing techniques and has high utility for both genome-wide and gene-focussed applications. Here we present the first individually cloned CRISPR-Cas9 genome wide arrayed sgRNA libraries covering 17,166 human and 20,430 mouse genes at a complexity of 34,332 sgRNAs for human and 40,860 sgRNAs for the mouse genome. For flexibility in generating stable cell lines the sgRNAs have been cloned in a lentivirus backbone containing PiggyBac transposase recognition elements together with fluorescent and drug selection markers. Over 95% of tested sgRNA induced specific DNA cleavage as measured by CEL-1 assays. Furthermore, sgRNA targeting GPI anchor protein pathway genes induced loss of function mutations in human and mouse cell lines measured by FLAER labelling. These arrayed libraries offer the prospect for performing screens on individual genes, combinations as well as larger gene sets. They also facilitate rapid deconvolution of signals from genome-wide screens. This set of vectors provide an organized comprehensive gene editing toolbox of considerable scientific value. More... »

PAGES

2244

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-017-01766-5

DOI

http://dx.doi.org/10.1038/s41598-017-01766-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1085458232

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28533524


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Wellcome Sanger Institute", 
          "id": "https://www.grid.ac/institutes/grid.10306.34", 
          "name": [
            "Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, CB10 1SA, Hinxton, Cambridge, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Metzakopian", 
        "givenName": "Emmanouil", 
        "id": "sg:person.01102423657.32", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01102423657.32"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Wellcome Sanger Institute", 
          "id": "https://www.grid.ac/institutes/grid.10306.34", 
          "name": [
            "Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, CB10 1SA, Hinxton, Cambridge, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Strong", 
        "givenName": "Alex", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Wellcome Sanger Institute", 
          "id": "https://www.grid.ac/institutes/grid.10306.34", 
          "name": [
            "Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, CB10 1SA, Hinxton, Cambridge, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Iyer", 
        "givenName": "Vivek", 
        "id": "sg:person.01153622414.26", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01153622414.26"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Wellcome Sanger Institute", 
          "id": "https://www.grid.ac/institutes/grid.10306.34", 
          "name": [
            "Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, CB10 1SA, Hinxton, Cambridge, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hodgkins", 
        "givenName": "Alex", 
        "id": "sg:person.01105507214.37", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01105507214.37"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Wellcome Sanger Institute", 
          "id": "https://www.grid.ac/institutes/grid.10306.34", 
          "name": [
            "Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, CB10 1SA, Hinxton, Cambridge, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tzelepis", 
        "givenName": "Konstantinos", 
        "id": "sg:person.011774672457.67", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011774672457.67"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Wellcome Sanger Institute", 
          "id": "https://www.grid.ac/institutes/grid.10306.34", 
          "name": [
            "Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, CB10 1SA, Hinxton, Cambridge, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Antunes", 
        "givenName": "Liliana", 
        "id": "sg:person.012572253057.75", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012572253057.75"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Wellcome Sanger Institute", 
          "id": "https://www.grid.ac/institutes/grid.10306.34", 
          "name": [
            "Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, CB10 1SA, Hinxton, Cambridge, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Friedrich", 
        "givenName": "Mathias J", 
        "id": "sg:person.07463350015.19", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07463350015.19"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "MilliporeSigma St. Louis, 2909 Laclede Ave, Missouri, USA", 
            "A Business of Merck KGaA, 64293, Darmstadt, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kang", 
        "givenName": "Qiaohua", 
        "id": "sg:person.01244046112.36", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01244046112.36"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "MilliporeSigma St. Louis, 2909 Laclede Ave, Missouri, USA", 
            "A Business of Merck KGaA, 64293, Darmstadt, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Davidson", 
        "givenName": "Teresa", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "MilliporeSigma St. Louis, 2909 Laclede Ave, Missouri, USA", 
            "A Business of Merck KGaA, 64293, Darmstadt, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lamberth", 
        "givenName": "Jacob", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "MilliporeSigma St. Louis, 2909 Laclede Ave, Missouri, USA", 
            "A Business of Merck KGaA, 64293, Darmstadt, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hoffmann", 
        "givenName": "Christina", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "MilliporeSigma St. Louis, 2909 Laclede Ave, Missouri, USA", 
            "A Business of Merck KGaA, 64293, Darmstadt, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Davis", 
        "givenName": "Gregory D.", 
        "id": "sg:person.0645256326.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0645256326.35"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Wellcome Sanger Institute", 
          "id": "https://www.grid.ac/institutes/grid.10306.34", 
          "name": [
            "Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, CB10 1SA, Hinxton, Cambridge, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Vassiliou", 
        "givenName": "George S.", 
        "id": "sg:person.01222767006.45", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01222767006.45"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Wellcome Sanger Institute", 
          "id": "https://www.grid.ac/institutes/grid.10306.34", 
          "name": [
            "Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, CB10 1SA, Hinxton, Cambridge, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Skarnes", 
        "givenName": "William C.", 
        "id": "sg:person.01241067364.28", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01241067364.28"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Wellcome Sanger Institute", 
          "id": "https://www.grid.ac/institutes/grid.10306.34", 
          "name": [
            "Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, CB10 1SA, Hinxton, Cambridge, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bradley", 
        "givenName": "Allan", 
        "id": "sg:person.01241363521.63", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01241363521.63"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/0076-6879(93)25054-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001008319"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tcb.2015.07.009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002077844"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/bioinformatics/btv308", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002798696"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0801017105", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006009044"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.7554/elife.12677", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006228897"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1247005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007966801"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1247005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007966801"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0138553", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009997150"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2105-6-31", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010551629", 
          "https://doi.org/10.1186/1471-2105-6-31"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2105-6-31", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010551629", 
          "https://doi.org/10.1186/1471-2105-6-31"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrg3899", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013619504", 
          "https://doi.org/10.1038/nrg3899"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1003674107", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015499030"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.celrep.2016.09.079", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015921711"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.1342", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018119119", 
          "https://doi.org/10.1038/nmeth.1342"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.1342", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018119119", 
          "https://doi.org/10.1038/nmeth.1342"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.21.1.289-297.2001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019605954"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/2159-8290.cd-16-0154", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022690314"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3389/fgene.2015.00362", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023533917"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.7554/elife.00471", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023633935"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.leu.2400768", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029179614", 
          "https://doi.org/10.1038/sj.leu.2400768"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.leu.2400768", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029179614", 
          "https://doi.org/10.1038/sj.leu.2400768"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/mic.0.023960-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034551304"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1138140", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036312168"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.062630599", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037130124"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2005.07.013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039880587"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3389/fgene.2015.00300", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040025592"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1216473109", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040822123"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature10413", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041325874", 
          "https://doi.org/10.1038/nature10413"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.leu.2402740", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041582176", 
          "https://doi.org/10.1038/sj.leu.2402740"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.leu.2402740", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041582176", 
          "https://doi.org/10.1038/sj.leu.2402740"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt.2800", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041802825", 
          "https://doi.org/10.1038/nbt.2800"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1225829", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041850060"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1008322108", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044126439"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkr764", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045552446"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1756-0500-1-28", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045919762", 
          "https://doi.org/10.1186/1756-0500-1-28"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/cyto.b.20151", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048279842"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/jb/mvn090", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059745959"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4161/cc.7.18.6679", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1072299623"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075091342", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2017-12", 
    "datePublishedReg": "2017-12-01", 
    "description": "CRISPR-Cas9 technology has accelerated biological research becoming routine for many laboratories. It is rapidly replacing conventional gene editing techniques and has high utility for both genome-wide and gene-focussed applications. Here we present the first individually cloned CRISPR-Cas9 genome wide arrayed sgRNA libraries covering 17,166 human and 20,430 mouse genes at a complexity of 34,332 sgRNAs for human and 40,860 sgRNAs for the mouse genome. For flexibility in generating stable cell lines the sgRNAs have been cloned in a lentivirus backbone containing PiggyBac transposase recognition elements together with fluorescent and drug selection markers. Over 95% of tested sgRNA induced specific DNA cleavage as measured by CEL-1 assays. Furthermore, sgRNA targeting GPI anchor protein pathway genes induced loss of function mutations in human and mouse cell lines measured by FLAER labelling. These arrayed libraries offer the prospect for performing screens on individual genes, combinations as well as larger gene sets. They also facilitate rapid deconvolution of signals from genome-wide screens. This set of vectors provide an organized comprehensive gene editing toolbox of considerable scientific value.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/s41598-017-01766-5", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.3498582", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1045337", 
        "issn": [
          "2045-2322"
        ], 
        "name": "Scientific Reports", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "7"
      }
    ], 
    "name": "Enhancing the genome editing toolbox: genome wide CRISPR arrayed libraries", 
    "pagination": "2244", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "16bb8297de477dce7940b07aec7cd252313eda03a2f7b76520bd86c0f0f82c2a"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "28533524"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101563288"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/s41598-017-01766-5"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1085458232"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/s41598-017-01766-5", 
      "https://app.dimensions.ai/details/publication/pub.1085458232"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T17:27", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8672_00000492.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/s41598-017-01766-5"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-017-01766-5'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-017-01766-5'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-017-01766-5'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-017-01766-5'


 

This table displays all metadata directly associated to this object as RDF triples.

288 TRIPLES      21 PREDICATES      63 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/s41598-017-01766-5 schema:about anzsrc-for:06
2 anzsrc-for:0604
3 schema:author Nd22ec0721a6d494b9bf2a7494a067052
4 schema:citation sg:pub.10.1038/nature10413
5 sg:pub.10.1038/nbt.2800
6 sg:pub.10.1038/nmeth.1342
7 sg:pub.10.1038/nrg3899
8 sg:pub.10.1038/sj.leu.2400768
9 sg:pub.10.1038/sj.leu.2402740
10 sg:pub.10.1186/1471-2105-6-31
11 sg:pub.10.1186/1756-0500-1-28
12 https://app.dimensions.ai/details/publication/pub.1075091342
13 https://doi.org/10.1002/cyto.b.20151
14 https://doi.org/10.1016/0076-6879(93)25054-6
15 https://doi.org/10.1016/j.cell.2005.07.013
16 https://doi.org/10.1016/j.celrep.2016.09.079
17 https://doi.org/10.1016/j.tcb.2015.07.009
18 https://doi.org/10.1073/pnas.062630599
19 https://doi.org/10.1073/pnas.0801017105
20 https://doi.org/10.1073/pnas.1003674107
21 https://doi.org/10.1073/pnas.1008322108
22 https://doi.org/10.1073/pnas.1216473109
23 https://doi.org/10.1093/bioinformatics/btv308
24 https://doi.org/10.1093/jb/mvn090
25 https://doi.org/10.1093/nar/gkr764
26 https://doi.org/10.1099/mic.0.023960-0
27 https://doi.org/10.1126/science.1138140
28 https://doi.org/10.1126/science.1225829
29 https://doi.org/10.1126/science.1247005
30 https://doi.org/10.1128/mcb.21.1.289-297.2001
31 https://doi.org/10.1158/2159-8290.cd-16-0154
32 https://doi.org/10.1371/journal.pone.0138553
33 https://doi.org/10.3389/fgene.2015.00300
34 https://doi.org/10.3389/fgene.2015.00362
35 https://doi.org/10.4161/cc.7.18.6679
36 https://doi.org/10.7554/elife.00471
37 https://doi.org/10.7554/elife.12677
38 schema:datePublished 2017-12
39 schema:datePublishedReg 2017-12-01
40 schema:description CRISPR-Cas9 technology has accelerated biological research becoming routine for many laboratories. It is rapidly replacing conventional gene editing techniques and has high utility for both genome-wide and gene-focussed applications. Here we present the first individually cloned CRISPR-Cas9 genome wide arrayed sgRNA libraries covering 17,166 human and 20,430 mouse genes at a complexity of 34,332 sgRNAs for human and 40,860 sgRNAs for the mouse genome. For flexibility in generating stable cell lines the sgRNAs have been cloned in a lentivirus backbone containing PiggyBac transposase recognition elements together with fluorescent and drug selection markers. Over 95% of tested sgRNA induced specific DNA cleavage as measured by CEL-1 assays. Furthermore, sgRNA targeting GPI anchor protein pathway genes induced loss of function mutations in human and mouse cell lines measured by FLAER labelling. These arrayed libraries offer the prospect for performing screens on individual genes, combinations as well as larger gene sets. They also facilitate rapid deconvolution of signals from genome-wide screens. This set of vectors provide an organized comprehensive gene editing toolbox of considerable scientific value.
41 schema:genre research_article
42 schema:inLanguage en
43 schema:isAccessibleForFree true
44 schema:isPartOf N41574046fc9f49e9a7dd984fd4969fb6
45 N5b7abe52269442c88ae632b2b28c1bc6
46 sg:journal.1045337
47 schema:name Enhancing the genome editing toolbox: genome wide CRISPR arrayed libraries
48 schema:pagination 2244
49 schema:productId N20fcf346bc5e4e72ab49392ecc766af3
50 N29ac4fd704334c73aa94e1f92db41834
51 N488d23ff923348f8afef0ea9458161a9
52 N78420225f59d4ad6a9eea732d47bd255
53 N9338717efbf147ed823674f01fad3a21
54 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085458232
55 https://doi.org/10.1038/s41598-017-01766-5
56 schema:sdDatePublished 2019-04-10T17:27
57 schema:sdLicense https://scigraph.springernature.com/explorer/license/
58 schema:sdPublisher N4ebc072f10554f6dbd4710c217458f04
59 schema:url https://www.nature.com/articles/s41598-017-01766-5
60 sgo:license sg:explorer/license/
61 sgo:sdDataset articles
62 rdf:type schema:ScholarlyArticle
63 N1843bcebd48a4722ba2a9defdbfd1b40 rdf:first sg:person.01105507214.37
64 rdf:rest N2b4c6bd8b67245cf898c99defbd9004c
65 N1d9ec7f8526b4e489cd702ea983a6665 schema:name A Business of Merck KGaA, 64293, Darmstadt, Germany
66 MilliporeSigma St. Louis, 2909 Laclede Ave, Missouri, USA
67 rdf:type schema:Organization
68 N1e670aa52e074847ad3f974002207431 schema:name A Business of Merck KGaA, 64293, Darmstadt, Germany
69 MilliporeSigma St. Louis, 2909 Laclede Ave, Missouri, USA
70 rdf:type schema:Organization
71 N20fcf346bc5e4e72ab49392ecc766af3 schema:name pubmed_id
72 schema:value 28533524
73 rdf:type schema:PropertyValue
74 N21398fb2ae804856bbb6eebbc6ae0731 rdf:first sg:person.01153622414.26
75 rdf:rest N1843bcebd48a4722ba2a9defdbfd1b40
76 N29ac4fd704334c73aa94e1f92db41834 schema:name nlm_unique_id
77 schema:value 101563288
78 rdf:type schema:PropertyValue
79 N2b4c6bd8b67245cf898c99defbd9004c rdf:first sg:person.011774672457.67
80 rdf:rest Ncd13d575754045ea8a03afc4647f91e5
81 N34ce5a0b09ce4ceaa2378832ef6d8adb rdf:first Na19de42ee5334053baec4137357a6d92
82 rdf:rest N21398fb2ae804856bbb6eebbc6ae0731
83 N3c448dec0fe9410ab7f19c17b8715f40 rdf:first Ne67e38bc5a4f4432b3082bcc1cc12aa1
84 rdf:rest N9aba055177c44cb68d9656acd417a016
85 N405cdce0f58448269671d3b5a949dde6 rdf:first sg:person.0645256326.35
86 rdf:rest Nef7e25732ae64e8aa7ede70e9230d081
87 N41574046fc9f49e9a7dd984fd4969fb6 schema:issueNumber 1
88 rdf:type schema:PublicationIssue
89 N488d23ff923348f8afef0ea9458161a9 schema:name dimensions_id
90 schema:value pub.1085458232
91 rdf:type schema:PropertyValue
92 N4ebc072f10554f6dbd4710c217458f04 schema:name Springer Nature - SN SciGraph project
93 rdf:type schema:Organization
94 N55fae29a4a634a2c883120612a6b9912 rdf:first sg:person.07463350015.19
95 rdf:rest Nf59cea5578ca45a590dc76a8f185808f
96 N5b7abe52269442c88ae632b2b28c1bc6 schema:volumeNumber 7
97 rdf:type schema:PublicationVolume
98 N624a050341a14d49b57872a05024a1e8 rdf:first sg:person.01241363521.63
99 rdf:rest rdf:nil
100 N6a09ac09b5234c738e46c099574fd899 schema:affiliation N93418c74127943eb93302dd75a3bcff6
101 schema:familyName Davidson
102 schema:givenName Teresa
103 rdf:type schema:Person
104 N78420225f59d4ad6a9eea732d47bd255 schema:name readcube_id
105 schema:value 16bb8297de477dce7940b07aec7cd252313eda03a2f7b76520bd86c0f0f82c2a
106 rdf:type schema:PropertyValue
107 N9338717efbf147ed823674f01fad3a21 schema:name doi
108 schema:value 10.1038/s41598-017-01766-5
109 rdf:type schema:PropertyValue
110 N93418c74127943eb93302dd75a3bcff6 schema:name A Business of Merck KGaA, 64293, Darmstadt, Germany
111 MilliporeSigma St. Louis, 2909 Laclede Ave, Missouri, USA
112 rdf:type schema:Organization
113 N9aba055177c44cb68d9656acd417a016 rdf:first Nd316cafb8b7a441eb25fb6450d4077ab
114 rdf:rest N405cdce0f58448269671d3b5a949dde6
115 N9c495b522d21436bb86e886f7a88af7e schema:name A Business of Merck KGaA, 64293, Darmstadt, Germany
116 MilliporeSigma St. Louis, 2909 Laclede Ave, Missouri, USA
117 rdf:type schema:Organization
118 Na19de42ee5334053baec4137357a6d92 schema:affiliation https://www.grid.ac/institutes/grid.10306.34
119 schema:familyName Strong
120 schema:givenName Alex
121 rdf:type schema:Person
122 Nc0eb41381e3647b0a2449ef4c222a836 rdf:first N6a09ac09b5234c738e46c099574fd899
123 rdf:rest N3c448dec0fe9410ab7f19c17b8715f40
124 Ncd13d575754045ea8a03afc4647f91e5 rdf:first sg:person.012572253057.75
125 rdf:rest N55fae29a4a634a2c883120612a6b9912
126 Ncd921230873d4508b6686a580f11f084 schema:name A Business of Merck KGaA, 64293, Darmstadt, Germany
127 MilliporeSigma St. Louis, 2909 Laclede Ave, Missouri, USA
128 rdf:type schema:Organization
129 Nd22ec0721a6d494b9bf2a7494a067052 rdf:first sg:person.01102423657.32
130 rdf:rest N34ce5a0b09ce4ceaa2378832ef6d8adb
131 Nd316cafb8b7a441eb25fb6450d4077ab schema:affiliation Ncd921230873d4508b6686a580f11f084
132 schema:familyName Hoffmann
133 schema:givenName Christina
134 rdf:type schema:Person
135 Ne67e38bc5a4f4432b3082bcc1cc12aa1 schema:affiliation N1d9ec7f8526b4e489cd702ea983a6665
136 schema:familyName Lamberth
137 schema:givenName Jacob
138 rdf:type schema:Person
139 Nef7e25732ae64e8aa7ede70e9230d081 rdf:first sg:person.01222767006.45
140 rdf:rest Nf667ddb66e9a4606ad04e1253b8f2ea6
141 Nf59cea5578ca45a590dc76a8f185808f rdf:first sg:person.01244046112.36
142 rdf:rest Nc0eb41381e3647b0a2449ef4c222a836
143 Nf667ddb66e9a4606ad04e1253b8f2ea6 rdf:first sg:person.01241067364.28
144 rdf:rest N624a050341a14d49b57872a05024a1e8
145 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
146 schema:name Biological Sciences
147 rdf:type schema:DefinedTerm
148 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
149 schema:name Genetics
150 rdf:type schema:DefinedTerm
151 sg:grant.3498582 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-017-01766-5
152 rdf:type schema:MonetaryGrant
153 sg:journal.1045337 schema:issn 2045-2322
154 schema:name Scientific Reports
155 rdf:type schema:Periodical
156 sg:person.01102423657.32 schema:affiliation https://www.grid.ac/institutes/grid.10306.34
157 schema:familyName Metzakopian
158 schema:givenName Emmanouil
159 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01102423657.32
160 rdf:type schema:Person
161 sg:person.01105507214.37 schema:affiliation https://www.grid.ac/institutes/grid.10306.34
162 schema:familyName Hodgkins
163 schema:givenName Alex
164 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01105507214.37
165 rdf:type schema:Person
166 sg:person.01153622414.26 schema:affiliation https://www.grid.ac/institutes/grid.10306.34
167 schema:familyName Iyer
168 schema:givenName Vivek
169 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01153622414.26
170 rdf:type schema:Person
171 sg:person.011774672457.67 schema:affiliation https://www.grid.ac/institutes/grid.10306.34
172 schema:familyName Tzelepis
173 schema:givenName Konstantinos
174 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011774672457.67
175 rdf:type schema:Person
176 sg:person.01222767006.45 schema:affiliation https://www.grid.ac/institutes/grid.10306.34
177 schema:familyName Vassiliou
178 schema:givenName George S.
179 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01222767006.45
180 rdf:type schema:Person
181 sg:person.01241067364.28 schema:affiliation https://www.grid.ac/institutes/grid.10306.34
182 schema:familyName Skarnes
183 schema:givenName William C.
184 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01241067364.28
185 rdf:type schema:Person
186 sg:person.01241363521.63 schema:affiliation https://www.grid.ac/institutes/grid.10306.34
187 schema:familyName Bradley
188 schema:givenName Allan
189 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01241363521.63
190 rdf:type schema:Person
191 sg:person.01244046112.36 schema:affiliation N1e670aa52e074847ad3f974002207431
192 schema:familyName Kang
193 schema:givenName Qiaohua
194 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01244046112.36
195 rdf:type schema:Person
196 sg:person.012572253057.75 schema:affiliation https://www.grid.ac/institutes/grid.10306.34
197 schema:familyName Antunes
198 schema:givenName Liliana
199 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012572253057.75
200 rdf:type schema:Person
201 sg:person.0645256326.35 schema:affiliation N9c495b522d21436bb86e886f7a88af7e
202 schema:familyName Davis
203 schema:givenName Gregory D.
204 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0645256326.35
205 rdf:type schema:Person
206 sg:person.07463350015.19 schema:affiliation https://www.grid.ac/institutes/grid.10306.34
207 schema:familyName Friedrich
208 schema:givenName Mathias J
209 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07463350015.19
210 rdf:type schema:Person
211 sg:pub.10.1038/nature10413 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041325874
212 https://doi.org/10.1038/nature10413
213 rdf:type schema:CreativeWork
214 sg:pub.10.1038/nbt.2800 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041802825
215 https://doi.org/10.1038/nbt.2800
216 rdf:type schema:CreativeWork
217 sg:pub.10.1038/nmeth.1342 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018119119
218 https://doi.org/10.1038/nmeth.1342
219 rdf:type schema:CreativeWork
220 sg:pub.10.1038/nrg3899 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013619504
221 https://doi.org/10.1038/nrg3899
222 rdf:type schema:CreativeWork
223 sg:pub.10.1038/sj.leu.2400768 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029179614
224 https://doi.org/10.1038/sj.leu.2400768
225 rdf:type schema:CreativeWork
226 sg:pub.10.1038/sj.leu.2402740 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041582176
227 https://doi.org/10.1038/sj.leu.2402740
228 rdf:type schema:CreativeWork
229 sg:pub.10.1186/1471-2105-6-31 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010551629
230 https://doi.org/10.1186/1471-2105-6-31
231 rdf:type schema:CreativeWork
232 sg:pub.10.1186/1756-0500-1-28 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045919762
233 https://doi.org/10.1186/1756-0500-1-28
234 rdf:type schema:CreativeWork
235 https://app.dimensions.ai/details/publication/pub.1075091342 schema:CreativeWork
236 https://doi.org/10.1002/cyto.b.20151 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048279842
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1016/0076-6879(93)25054-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001008319
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1016/j.cell.2005.07.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039880587
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1016/j.celrep.2016.09.079 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015921711
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1016/j.tcb.2015.07.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002077844
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1073/pnas.062630599 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037130124
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1073/pnas.0801017105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006009044
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1073/pnas.1003674107 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015499030
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1073/pnas.1008322108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044126439
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1073/pnas.1216473109 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040822123
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1093/bioinformatics/btv308 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002798696
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1093/jb/mvn090 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059745959
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1093/nar/gkr764 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045552446
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1099/mic.0.023960-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034551304
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1126/science.1138140 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036312168
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1126/science.1225829 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041850060
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1126/science.1247005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007966801
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1128/mcb.21.1.289-297.2001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019605954
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1158/2159-8290.cd-16-0154 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022690314
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1371/journal.pone.0138553 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009997150
275 rdf:type schema:CreativeWork
276 https://doi.org/10.3389/fgene.2015.00300 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040025592
277 rdf:type schema:CreativeWork
278 https://doi.org/10.3389/fgene.2015.00362 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023533917
279 rdf:type schema:CreativeWork
280 https://doi.org/10.4161/cc.7.18.6679 schema:sameAs https://app.dimensions.ai/details/publication/pub.1072299623
281 rdf:type schema:CreativeWork
282 https://doi.org/10.7554/elife.00471 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023633935
283 rdf:type schema:CreativeWork
284 https://doi.org/10.7554/elife.12677 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006228897
285 rdf:type schema:CreativeWork
286 https://www.grid.ac/institutes/grid.10306.34 schema:alternateName Wellcome Sanger Institute
287 schema:name Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, CB10 1SA, Hinxton, Cambridge, UK
288 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...