Holographic two-photon activation for synthetic optogenetics View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2019-03

AUTHORS

Ido Carmi, Marco De Battista, Laura Maddalena, Elizabeth C. Carroll, Michael A. Kienzler, Shai Berlin

ABSTRACT

Optogenetic tools provide users the ability to photocontrol the activity of cells. Commonly, activation is achieved by expression of proteins from photosynthetic organisms, for example, microbial opsins (e.g., ChR2). Alternatively, a sister approach, synthetic optogenetics, enables photocontrol over proteins of mammalian origin by use of photoswitches, visible light (typically), and genetic modification. Thus, synthetic optogenetics facilitates interrogation of native neuronal signaling mechanisms. However, the poor tissue penetration of visible wavelengths impedes the use of the technique in tissue, as two-photon excitation (2PE) is typically required to access the near-infrared window. Here, we describe an alternative technique that uses 2PE-compatible photoswitches (section 1) for photoactivation of genetically modified glutamate receptors (section 2). Furthermore, for fast, multi-region photoactivation, we describe the use of 2P-digital holography (2P-DH) (section 3). We detail how to combine 2P-DH and synthetic optogenetics with electrophysiology, or with red fluorescence Ca2+ recordings, for all-optical neural interrogation. The time required to complete the methods, aside from obtaining the necessary reagents and illumination equipment, is ~3 weeks. More... »

PAGES

864-900

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41596-018-0118-2

DOI

http://dx.doi.org/10.1038/s41596-018-0118-2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1112365553

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30804570


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