Ontology type: schema:ScholarlyArticle Open Access: True
2021-04-29
AUTHORSHa V. Dang, Robert W. Cross, Viktoriya Borisevich, Zachary A. Bornholdt, Brandyn R. West, Yee-Peng Chan, Chad E. Mire, Sofia Cheliout Da Silva, Antony S. Dimitrov, Lianying Yan, Moushimi Amaya, Chanakha K. Navaratnarajah, Larry Zeitlin, Thomas W. Geisbert, Christopher C. Broder, David Veesler
ABSTRACTHendra virus (HeV) and Nipah virus (NiV) are henipaviruses (HNVs) causing respiratory illness and severe encephalitis in humans, with fatality rates of 50–100%. There are no licensed therapeutics or vaccines to protect humans. HeV and NiV use a receptor-binding glycoprotein (G) and a fusion glycoprotein (F) to enter host cells. HNV F and G are the main targets of the humoral immune response, and the presence of neutralizing antibodies is a correlate of protection against NiV and HeV in experimentally infected animals. We describe here two cross-reactive F-specific antibodies, 1F5 and 12B2, that neutralize NiV and HeV through inhibition of membrane fusion. Cryo-electron microscopy structures reveal that 1F5 and 12B2 recognize distinct prefusion-specific, conserved quaternary epitopes and lock F in its prefusion conformation. We provide proof-of-concept for using antibody cocktails for neutralizing NiV and HeV and define a roadmap for developing effective countermeasures against these highly pathogenic viruses. More... »
PAGES426-434
http://scigraph.springernature.com/pub.10.1038/s41594-021-00584-8
DOIhttp://dx.doi.org/10.1038/s41594-021-00584-8
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1137603226
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/33927387
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Medical and Health Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1107",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Immunology",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Medical Microbiology",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Animals",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Antibodies, Monoclonal, Humanized",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Antibodies, Viral",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Broadly Neutralizing Antibodies",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "CHO Cells",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cricetulus",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cross Reactions",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "HEK293 Cells",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Hendra Virus",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Henipavirus Infections",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Humans",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mice",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Nipah Virus",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Viral Fusion Proteins",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Virus Internalization",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Department of Biochemistry, University of Washington, Seattle, WA, USA",
"id": "http://www.grid.ac/institutes/grid.34477.33",
"name": [
"Department of Biochemistry, University of Washington, Seattle, WA, USA"
],
"type": "Organization"
},
"familyName": "Dang",
"givenName": "Ha V.",
"id": "sg:person.012756216443.47",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012756216443.47"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Galveston National Laboratory, University of Texas Medical Branch, Galveston, TX, USA",
"id": "http://www.grid.ac/institutes/grid.176731.5",
"name": [
"Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA",
"Galveston National Laboratory, University of Texas Medical Branch, Galveston, TX, USA"
],
"type": "Organization"
},
"familyName": "Cross",
"givenName": "Robert W.",
"id": "sg:person.01321077225.18",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01321077225.18"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA",
"id": "http://www.grid.ac/institutes/grid.176731.5",
"name": [
"Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA"
],
"type": "Organization"
},
"familyName": "Borisevich",
"givenName": "Viktoriya",
"id": "sg:person.013226655707.49",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013226655707.49"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Mapp Biopharmaceutical, Inc., San Diego, CA, USA",
"id": "http://www.grid.ac/institutes/grid.421122.6",
"name": [
"Mapp Biopharmaceutical, Inc., San Diego, CA, USA"
],
"type": "Organization"
},
"familyName": "Bornholdt",
"givenName": "Zachary A.",
"id": "sg:person.01361432261.18",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01361432261.18"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Mapp Biopharmaceutical, Inc., San Diego, CA, USA",
"id": "http://www.grid.ac/institutes/grid.421122.6",
"name": [
"Mapp Biopharmaceutical, Inc., San Diego, CA, USA"
],
"type": "Organization"
},
"familyName": "West",
"givenName": "Brandyn R.",
"id": "sg:person.010005727760.88",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010005727760.88"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA",
"id": "http://www.grid.ac/institutes/grid.265436.0",
"name": [
"Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA"
],
"type": "Organization"
},
"familyName": "Chan",
"givenName": "Yee-Peng",
"id": "sg:person.01200117555.35",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01200117555.35"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Galveston National Laboratory, University of Texas Medical Branch, Galveston, TX, USA",
"id": "http://www.grid.ac/institutes/grid.176731.5",
"name": [
"Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA",
"Galveston National Laboratory, University of Texas Medical Branch, Galveston, TX, USA"
],
"type": "Organization"
},
"familyName": "Mire",
"givenName": "Chad E.",
"id": "sg:person.0705360330.66",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0705360330.66"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA",
"id": "http://www.grid.ac/institutes/grid.265436.0",
"name": [
"Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA"
],
"type": "Organization"
},
"familyName": "Da Silva",
"givenName": "Sofia Cheliout",
"id": "sg:person.014561463261.50",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014561463261.50"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA",
"id": "http://www.grid.ac/institutes/grid.265436.0",
"name": [
"Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA"
],
"type": "Organization"
},
"familyName": "Dimitrov",
"givenName": "Antony S.",
"id": "sg:person.01327700701.29",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01327700701.29"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA",
"id": "http://www.grid.ac/institutes/grid.265436.0",
"name": [
"Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA"
],
"type": "Organization"
},
"familyName": "Yan",
"givenName": "Lianying",
"id": "sg:person.0647037406.00",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0647037406.00"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA",
"id": "http://www.grid.ac/institutes/grid.265436.0",
"name": [
"Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA"
],
"type": "Organization"
},
"familyName": "Amaya",
"givenName": "Moushimi",
"id": "sg:person.0630057102.41",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0630057102.41"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Molecular Medicine, Mayo Clinic, Rochester, MN, USA",
"id": "http://www.grid.ac/institutes/grid.66875.3a",
"name": [
"Department of Molecular Medicine, Mayo Clinic, Rochester, MN, USA"
],
"type": "Organization"
},
"familyName": "Navaratnarajah",
"givenName": "Chanakha K.",
"id": "sg:person.0606553260.52",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0606553260.52"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Mapp Biopharmaceutical, Inc., San Diego, CA, USA",
"id": "http://www.grid.ac/institutes/grid.421122.6",
"name": [
"Mapp Biopharmaceutical, Inc., San Diego, CA, USA"
],
"type": "Organization"
},
"familyName": "Zeitlin",
"givenName": "Larry",
"id": "sg:person.01175422023.99",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01175422023.99"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Galveston National Laboratory, University of Texas Medical Branch, Galveston, TX, USA",
"id": "http://www.grid.ac/institutes/grid.176731.5",
"name": [
"Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA",
"Galveston National Laboratory, University of Texas Medical Branch, Galveston, TX, USA"
],
"type": "Organization"
},
"familyName": "Geisbert",
"givenName": "Thomas W.",
"id": "sg:person.014466116617.16",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014466116617.16"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA",
"id": "http://www.grid.ac/institutes/grid.265436.0",
"name": [
"Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA"
],
"type": "Organization"
},
"familyName": "Broder",
"givenName": "Christopher C.",
"id": "sg:person.0614131467.47",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0614131467.47"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Biochemistry, University of Washington, Seattle, WA, USA",
"id": "http://www.grid.ac/institutes/grid.34477.33",
"name": [
"Department of Biochemistry, University of Washington, Seattle, WA, USA"
],
"type": "Organization"
},
"familyName": "Veesler",
"givenName": "David",
"id": "sg:person.01002414777.95",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01002414777.95"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1038/nmeth.3541",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004871597",
"https://doi.org/10.1038/nmeth.3541"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nmeth.4193",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1084129286",
"https://doi.org/10.1038/nmeth.4193"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/ncomms6342",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1028480583",
"https://doi.org/10.1038/ncomms6342"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nmeth.2115",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1041024070",
"https://doi.org/10.1038/nmeth.2115"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/s41586-020-2349-y",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1127684942",
"https://doi.org/10.1038/s41586-020-2349-y"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/82_2012_207",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1007852348",
"https://doi.org/10.1007/82_2012_207"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/s12985-018-0964-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1101776899",
"https://doi.org/10.1186/s12985-018-0964-0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/ncomms1796",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1043718269",
"https://doi.org/10.1038/ncomms1796"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/s41594-019-0308-9",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1121383201",
"https://doi.org/10.1038/s41594-019-0308-9"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nrmicro1323",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1021039329",
"https://doi.org/10.1038/nrmicro1323"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nmeth.4169",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1083686092",
"https://doi.org/10.1038/nmeth.4169"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature03838",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1031943292",
"https://doi.org/10.1038/nature03838"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nmeth.3286",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1015110469",
"https://doi.org/10.1038/nmeth.3286"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/978-3-540-72146-8_3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1015864377",
"https://doi.org/10.1007/978-3-540-72146-8_3"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nsmb.3115",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010755414",
"https://doi.org/10.1038/nsmb.3115"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nsmb.1435",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044123311",
"https://doi.org/10.1038/nsmb.1435"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/s41467-017-00863-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1092015759",
"https://doi.org/10.1038/s41467-017-00863-3"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/s41592-020-00990-8",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1132982567",
"https://doi.org/10.1038/s41592-020-00990-8"
],
"type": "CreativeWork"
}
],
"datePublished": "2021-04-29",
"datePublishedReg": "2021-04-29",
"description": "Hendra virus (HeV) and Nipah virus (NiV) are henipaviruses (HNVs) causing respiratory illness and severe encephalitis in humans, with fatality rates of 50\u2013100%. There are no licensed therapeutics or vaccines to protect humans. HeV and NiV use a receptor-binding glycoprotein (G) and a fusion glycoprotein (F) to enter host cells. HNV F and G are the main targets of the humoral immune response, and the presence of neutralizing antibodies is a correlate of protection against NiV and HeV in experimentally infected animals. We describe here two cross-reactive F-specific antibodies, 1F5 and 12B2, that neutralize NiV and HeV through inhibition of membrane fusion. Cryo-electron microscopy structures reveal that 1F5 and 12B2 recognize distinct prefusion-specific, conserved quaternary epitopes and lock F in its prefusion conformation. We provide proof-of-concept for using antibody cocktails for neutralizing NiV and HeV and define a roadmap for developing effective countermeasures against these highly pathogenic viruses.",
"genre": "article",
"id": "sg:pub.10.1038/s41594-021-00584-8",
"inLanguage": "en",
"isAccessibleForFree": true,
"isFundedItemOf": [
{
"id": "sg:grant.7169995",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.2688023",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.2456178",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.9534495",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.2704770",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.5503638",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.6803473",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.8387643",
"type": "MonetaryGrant"
}
],
"isPartOf": [
{
"id": "sg:journal.1295033",
"issn": [
"1545-9993",
"2331-365X"
],
"name": "Nature Structural & Molecular Biology",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "5",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "28"
}
],
"keywords": [
"antibody cocktail",
"correlates of protection",
"Nipah virus",
"humoral immune response",
"licensed therapeutics",
"respiratory illness",
"severe encephalitis",
"fusion glycoproteins",
"immune response",
"quaternary epitopes",
"fatality rate",
"specific antibodies",
"virus",
"NIV",
"receptor-binding glycoprotein",
"prefusion conformation",
"Hendra virus",
"pathogenic viruses",
"host cells",
"antibodies",
"henipaviruses",
"HEV",
"glycoprotein",
"encephalitis",
"humans",
"vaccine",
"illness",
"cocktail",
"main target",
"epitopes",
"therapeutics",
"inhibition",
"animals",
"correlates",
"effective countermeasures",
"cells",
"response",
"membrane fusion",
"target",
"rate",
"cryo-electron microscopy structure",
"presence",
"protection",
"fusion",
"microscopy structure",
"countermeasures",
"roadmap",
"proof",
"concept",
"structure",
"conformation"
],
"name": "Broadly neutralizing antibody cocktails targeting Nipah virus and Hendra virus fusion glycoproteins",
"pagination": "426-434",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1137603226"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1038/s41594-021-00584-8"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"33927387"
]
}
],
"sameAs": [
"https://doi.org/10.1038/s41594-021-00584-8",
"https://app.dimensions.ai/details/publication/pub.1137603226"
],
"sdDataset": "articles",
"sdDatePublished": "2022-06-01T22:24",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220601/entities/gbq_results/article/article_911.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1038/s41594-021-00584-8"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41594-021-00584-8'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41594-021-00584-8'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41594-021-00584-8'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41594-021-00584-8'
This table displays all metadata directly associated to this object as RDF triples.
384 TRIPLES
22 PREDICATES
111 URIs
84 LITERALS
22 BLANK NODES