Broadly neutralizing antibody cocktails targeting Nipah virus and Hendra virus fusion glycoproteins View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2021-04-29

AUTHORS

Ha V. Dang, Robert W. Cross, Viktoriya Borisevich, Zachary A. Bornholdt, Brandyn R. West, Yee-Peng Chan, Chad E. Mire, Sofia Cheliout Da Silva, Antony S. Dimitrov, Lianying Yan, Moushimi Amaya, Chanakha K. Navaratnarajah, Larry Zeitlin, Thomas W. Geisbert, Christopher C. Broder, David Veesler

ABSTRACT

Hendra virus (HeV) and Nipah virus (NiV) are henipaviruses (HNVs) causing respiratory illness and severe encephalitis in humans, with fatality rates of 50–100%. There are no licensed therapeutics or vaccines to protect humans. HeV and NiV use a receptor-binding glycoprotein (G) and a fusion glycoprotein (F) to enter host cells. HNV F and G are the main targets of the humoral immune response, and the presence of neutralizing antibodies is a correlate of protection against NiV and HeV in experimentally infected animals. We describe here two cross-reactive F-specific antibodies, 1F5 and 12B2, that neutralize NiV and HeV through inhibition of membrane fusion. Cryo-electron microscopy structures reveal that 1F5 and 12B2 recognize distinct prefusion-specific, conserved quaternary epitopes and lock F in its prefusion conformation. We provide proof-of-concept for using antibody cocktails for neutralizing NiV and HeV and define a roadmap for developing effective countermeasures against these highly pathogenic viruses. More... »

PAGES

426-434

References to SciGraph publications

  • 2012-07-29. Prevention of overfitting in cryo-EM structure determination in NATURE METHODS
  • 2019-09-30. An antibody against the F glycoprotein inhibits Nipah and Hendra virus infections in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • 2020-11-30. Non-uniform refinement: adaptive regularization improves single-particle cryo-EM reconstruction in NATURE METHODS
  • 2018-03-27. Rescue and characterization of recombinant cedar virus, a non-pathogenic Henipavirus species in VIROLOGY JOURNAL
  • 2017-02-06. cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination in NATURE METHODS
  • 2005-07-06. EphrinB2 is the entry receptor for Nipah virus, an emergent deadly paramyxovirus in NATURE
  • 2008-05-18. Structural basis of Nipah and Hendra virus attachment to their cell-surface receptor ephrin-B2 in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • 2017-02-27. MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy in NATURE METHODS
  • 2012-07-01. Epidemiology of Henipavirus Disease in Humans in HENIPAVIRUS
  • 2017-10-03. Monomeric ephrinB2 binding induces allosteric changes in Nipah virus G that precede its full activation in NATURE COMMUNICATIONS
  • 2015-08-17. EMRinger: side chain–directed model and map validation for 3D cryo-electron microscopy in NATURE METHODS
  • 2015-11-04. Privateer: software for the conformational validation of carbohydrate structures in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • 2008. Human Monoclonal Antibody and Vaccine Approaches to Prevent Human Rabies in HUMAN ANTIBODY THERAPEUTICS FOR VIRAL DISEASE
  • 2014-11-18. Evidence for henipavirus spillover into human populations in Africa in NATURE COMMUNICATIONS
  • 2006-01. Hendra and Nipah viruses: different and dangerous in NATURE REVIEWS MICROBIOLOGY
  • 2020-05-18. Cross-neutralization of SARS-CoV-2 by a human monoclonal SARS-CoV antibody in NATURE
  • 2015-02-23. Atomic-accuracy models from 4.5-Å cryo-electron microscopy data with density-guided iterative local refinement in NATURE METHODS
  • 2012-01. Bats host major mammalian paramyxoviruses in NATURE COMMUNICATIONS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41594-021-00584-8

    DOI

    http://dx.doi.org/10.1038/s41594-021-00584-8

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1137603226

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/33927387


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical and Health Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1107", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Immunology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical Microbiology", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Antibodies, Monoclonal, Humanized", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Antibodies, Viral", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Broadly Neutralizing Antibodies", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "CHO Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cricetulus", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cross Reactions", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "HEK293 Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Hendra Virus", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Henipavirus Infections", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Nipah Virus", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Viral Fusion Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Virus Internalization", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Department of Biochemistry, University of Washington, Seattle, WA, USA", 
              "id": "http://www.grid.ac/institutes/grid.34477.33", 
              "name": [
                "Department of Biochemistry, University of Washington, Seattle, WA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Dang", 
            "givenName": "Ha V.", 
            "id": "sg:person.012756216443.47", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012756216443.47"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Galveston National Laboratory, University of Texas Medical Branch, Galveston, TX, USA", 
              "id": "http://www.grid.ac/institutes/grid.176731.5", 
              "name": [
                "Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA", 
                "Galveston National Laboratory, University of Texas Medical Branch, Galveston, TX, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Cross", 
            "givenName": "Robert W.", 
            "id": "sg:person.01321077225.18", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01321077225.18"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA", 
              "id": "http://www.grid.ac/institutes/grid.176731.5", 
              "name": [
                "Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Borisevich", 
            "givenName": "Viktoriya", 
            "id": "sg:person.013226655707.49", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013226655707.49"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Mapp Biopharmaceutical, Inc., San Diego, CA, USA", 
              "id": "http://www.grid.ac/institutes/grid.421122.6", 
              "name": [
                "Mapp Biopharmaceutical, Inc., San Diego, CA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Bornholdt", 
            "givenName": "Zachary A.", 
            "id": "sg:person.01361432261.18", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01361432261.18"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Mapp Biopharmaceutical, Inc., San Diego, CA, USA", 
              "id": "http://www.grid.ac/institutes/grid.421122.6", 
              "name": [
                "Mapp Biopharmaceutical, Inc., San Diego, CA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "West", 
            "givenName": "Brandyn R.", 
            "id": "sg:person.010005727760.88", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010005727760.88"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA", 
              "id": "http://www.grid.ac/institutes/grid.265436.0", 
              "name": [
                "Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Chan", 
            "givenName": "Yee-Peng", 
            "id": "sg:person.01200117555.35", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01200117555.35"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Galveston National Laboratory, University of Texas Medical Branch, Galveston, TX, USA", 
              "id": "http://www.grid.ac/institutes/grid.176731.5", 
              "name": [
                "Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA", 
                "Galveston National Laboratory, University of Texas Medical Branch, Galveston, TX, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Mire", 
            "givenName": "Chad E.", 
            "id": "sg:person.0705360330.66", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0705360330.66"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA", 
              "id": "http://www.grid.ac/institutes/grid.265436.0", 
              "name": [
                "Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Da Silva", 
            "givenName": "Sofia Cheliout", 
            "id": "sg:person.014561463261.50", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014561463261.50"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA", 
              "id": "http://www.grid.ac/institutes/grid.265436.0", 
              "name": [
                "Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Dimitrov", 
            "givenName": "Antony S.", 
            "id": "sg:person.01327700701.29", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01327700701.29"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA", 
              "id": "http://www.grid.ac/institutes/grid.265436.0", 
              "name": [
                "Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Yan", 
            "givenName": "Lianying", 
            "id": "sg:person.0647037406.00", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0647037406.00"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA", 
              "id": "http://www.grid.ac/institutes/grid.265436.0", 
              "name": [
                "Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Amaya", 
            "givenName": "Moushimi", 
            "id": "sg:person.0630057102.41", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0630057102.41"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Molecular Medicine, Mayo Clinic, Rochester, MN, USA", 
              "id": "http://www.grid.ac/institutes/grid.66875.3a", 
              "name": [
                "Department of Molecular Medicine, Mayo Clinic, Rochester, MN, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Navaratnarajah", 
            "givenName": "Chanakha K.", 
            "id": "sg:person.0606553260.52", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0606553260.52"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Mapp Biopharmaceutical, Inc., San Diego, CA, USA", 
              "id": "http://www.grid.ac/institutes/grid.421122.6", 
              "name": [
                "Mapp Biopharmaceutical, Inc., San Diego, CA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zeitlin", 
            "givenName": "Larry", 
            "id": "sg:person.01175422023.99", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01175422023.99"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Galveston National Laboratory, University of Texas Medical Branch, Galveston, TX, USA", 
              "id": "http://www.grid.ac/institutes/grid.176731.5", 
              "name": [
                "Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA", 
                "Galveston National Laboratory, University of Texas Medical Branch, Galveston, TX, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Geisbert", 
            "givenName": "Thomas W.", 
            "id": "sg:person.014466116617.16", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014466116617.16"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA", 
              "id": "http://www.grid.ac/institutes/grid.265436.0", 
              "name": [
                "Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Broder", 
            "givenName": "Christopher C.", 
            "id": "sg:person.0614131467.47", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0614131467.47"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Biochemistry, University of Washington, Seattle, WA, USA", 
              "id": "http://www.grid.ac/institutes/grid.34477.33", 
              "name": [
                "Department of Biochemistry, University of Washington, Seattle, WA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Veesler", 
            "givenName": "David", 
            "id": "sg:person.01002414777.95", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01002414777.95"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/nmeth.3541", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004871597", 
              "https://doi.org/10.1038/nmeth.3541"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.4193", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1084129286", 
              "https://doi.org/10.1038/nmeth.4193"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ncomms6342", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028480583", 
              "https://doi.org/10.1038/ncomms6342"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.2115", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041024070", 
              "https://doi.org/10.1038/nmeth.2115"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/s41586-020-2349-y", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1127684942", 
              "https://doi.org/10.1038/s41586-020-2349-y"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/82_2012_207", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007852348", 
              "https://doi.org/10.1007/82_2012_207"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12985-018-0964-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1101776899", 
              "https://doi.org/10.1186/s12985-018-0964-0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ncomms1796", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043718269", 
              "https://doi.org/10.1038/ncomms1796"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/s41594-019-0308-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1121383201", 
              "https://doi.org/10.1038/s41594-019-0308-9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrmicro1323", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021039329", 
              "https://doi.org/10.1038/nrmicro1323"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.4169", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1083686092", 
              "https://doi.org/10.1038/nmeth.4169"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature03838", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031943292", 
              "https://doi.org/10.1038/nature03838"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.3286", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015110469", 
              "https://doi.org/10.1038/nmeth.3286"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-3-540-72146-8_3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015864377", 
              "https://doi.org/10.1007/978-3-540-72146-8_3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nsmb.3115", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010755414", 
              "https://doi.org/10.1038/nsmb.3115"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nsmb.1435", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044123311", 
              "https://doi.org/10.1038/nsmb.1435"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/s41467-017-00863-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1092015759", 
              "https://doi.org/10.1038/s41467-017-00863-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/s41592-020-00990-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1132982567", 
              "https://doi.org/10.1038/s41592-020-00990-8"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2021-04-29", 
        "datePublishedReg": "2021-04-29", 
        "description": "Hendra virus (HeV) and Nipah virus (NiV) are henipaviruses (HNVs) causing respiratory illness and severe encephalitis in humans, with fatality rates of 50\u2013100%. There are no licensed therapeutics or vaccines to protect humans. HeV and NiV use a receptor-binding glycoprotein (G) and a fusion glycoprotein (F) to enter host cells. HNV F and G are the main targets of the humoral immune response, and the presence of neutralizing antibodies is a correlate of protection against NiV and HeV in experimentally infected animals. We describe here two cross-reactive F-specific antibodies, 1F5 and 12B2, that neutralize NiV and HeV through inhibition of membrane fusion. Cryo-electron microscopy structures reveal that 1F5 and 12B2 recognize distinct prefusion-specific, conserved quaternary epitopes and lock F in its prefusion conformation. We provide proof-of-concept for using antibody cocktails for neutralizing NiV and HeV and define a roadmap for developing effective countermeasures against these highly pathogenic viruses.", 
        "genre": "article", 
        "id": "sg:pub.10.1038/s41594-021-00584-8", 
        "inLanguage": "en", 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.7169995", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2688023", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2456178", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.9534495", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2704770", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.5503638", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.6803473", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.8387643", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1295033", 
            "issn": [
              "1545-9993", 
              "2331-365X"
            ], 
            "name": "Nature Structural & Molecular Biology", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "5", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "28"
          }
        ], 
        "keywords": [
          "antibody cocktail", 
          "correlates of protection", 
          "Nipah virus", 
          "humoral immune response", 
          "licensed therapeutics", 
          "respiratory illness", 
          "severe encephalitis", 
          "fusion glycoproteins", 
          "immune response", 
          "quaternary epitopes", 
          "fatality rate", 
          "specific antibodies", 
          "virus", 
          "NIV", 
          "receptor-binding glycoprotein", 
          "prefusion conformation", 
          "Hendra virus", 
          "pathogenic viruses", 
          "host cells", 
          "antibodies", 
          "henipaviruses", 
          "HEV", 
          "glycoprotein", 
          "encephalitis", 
          "humans", 
          "vaccine", 
          "illness", 
          "cocktail", 
          "main target", 
          "epitopes", 
          "therapeutics", 
          "inhibition", 
          "animals", 
          "correlates", 
          "effective countermeasures", 
          "cells", 
          "response", 
          "membrane fusion", 
          "target", 
          "rate", 
          "cryo-electron microscopy structure", 
          "presence", 
          "protection", 
          "fusion", 
          "microscopy structure", 
          "countermeasures", 
          "roadmap", 
          "proof", 
          "concept", 
          "structure", 
          "conformation"
        ], 
        "name": "Broadly neutralizing antibody cocktails targeting Nipah virus and Hendra virus fusion glycoproteins", 
        "pagination": "426-434", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1137603226"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/s41594-021-00584-8"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "33927387"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/s41594-021-00584-8", 
          "https://app.dimensions.ai/details/publication/pub.1137603226"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-06-01T22:24", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20220601/entities/gbq_results/article/article_911.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1038/s41594-021-00584-8"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41594-021-00584-8'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41594-021-00584-8'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41594-021-00584-8'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41594-021-00584-8'


     

    This table displays all metadata directly associated to this object as RDF triples.

    384 TRIPLES      22 PREDICATES      111 URIs      84 LITERALS      22 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/s41594-021-00584-8 schema:about N04fcd4d656e349bbbd26d42cc861146a
    2 N167b7546e6f64efb93c161861b1a7774
    3 N21fd34d74ec943dab9e3dd7654768d64
    4 N2fc5bb76748d4456a360a94a4d9199eb
    5 N448cd0d714b347cdbc8da82f7b2d2a56
    6 N4bd006621c444f018053daf603b642df
    7 N6e8f949ecdde4b229fc4d1cf0bad3027
    8 N8e6f643f5a11419588521de763da8259
    9 N909803e523d14592bac9f6f682e8e597
    10 N92c70dc8643548f8afe6424b80085082
    11 Nb1d358e33271456ca026f524d5517a79
    12 Nb33ae07c38744c3aa4117c12581326e0
    13 Nb778557f35564364a0ecd7f3c99611a0
    14 Nbaef3fe53bff4e86884d348832b81b77
    15 Ndd65c16011be49968e9da9c9ef7da93d
    16 anzsrc-for:11
    17 anzsrc-for:1107
    18 anzsrc-for:1108
    19 schema:author N3129fff0dda943a3bca68a6589daef79
    20 schema:citation sg:pub.10.1007/82_2012_207
    21 sg:pub.10.1007/978-3-540-72146-8_3
    22 sg:pub.10.1038/nature03838
    23 sg:pub.10.1038/ncomms1796
    24 sg:pub.10.1038/ncomms6342
    25 sg:pub.10.1038/nmeth.2115
    26 sg:pub.10.1038/nmeth.3286
    27 sg:pub.10.1038/nmeth.3541
    28 sg:pub.10.1038/nmeth.4169
    29 sg:pub.10.1038/nmeth.4193
    30 sg:pub.10.1038/nrmicro1323
    31 sg:pub.10.1038/nsmb.1435
    32 sg:pub.10.1038/nsmb.3115
    33 sg:pub.10.1038/s41467-017-00863-3
    34 sg:pub.10.1038/s41586-020-2349-y
    35 sg:pub.10.1038/s41592-020-00990-8
    36 sg:pub.10.1038/s41594-019-0308-9
    37 sg:pub.10.1186/s12985-018-0964-0
    38 schema:datePublished 2021-04-29
    39 schema:datePublishedReg 2021-04-29
    40 schema:description Hendra virus (HeV) and Nipah virus (NiV) are henipaviruses (HNVs) causing respiratory illness and severe encephalitis in humans, with fatality rates of 50–100%. There are no licensed therapeutics or vaccines to protect humans. HeV and NiV use a receptor-binding glycoprotein (G) and a fusion glycoprotein (F) to enter host cells. HNV F and G are the main targets of the humoral immune response, and the presence of neutralizing antibodies is a correlate of protection against NiV and HeV in experimentally infected animals. We describe here two cross-reactive F-specific antibodies, 1F5 and 12B2, that neutralize NiV and HeV through inhibition of membrane fusion. Cryo-electron microscopy structures reveal that 1F5 and 12B2 recognize distinct prefusion-specific, conserved quaternary epitopes and lock F in its prefusion conformation. We provide proof-of-concept for using antibody cocktails for neutralizing NiV and HeV and define a roadmap for developing effective countermeasures against these highly pathogenic viruses.
    41 schema:genre article
    42 schema:inLanguage en
    43 schema:isAccessibleForFree true
    44 schema:isPartOf N77117d493cf54ac58df072eaca86d17c
    45 Nbeef81381e684078992c22cdcb6510f8
    46 sg:journal.1295033
    47 schema:keywords HEV
    48 Hendra virus
    49 NIV
    50 Nipah virus
    51 animals
    52 antibodies
    53 antibody cocktail
    54 cells
    55 cocktail
    56 concept
    57 conformation
    58 correlates
    59 correlates of protection
    60 countermeasures
    61 cryo-electron microscopy structure
    62 effective countermeasures
    63 encephalitis
    64 epitopes
    65 fatality rate
    66 fusion
    67 fusion glycoproteins
    68 glycoprotein
    69 henipaviruses
    70 host cells
    71 humans
    72 humoral immune response
    73 illness
    74 immune response
    75 inhibition
    76 licensed therapeutics
    77 main target
    78 membrane fusion
    79 microscopy structure
    80 pathogenic viruses
    81 prefusion conformation
    82 presence
    83 proof
    84 protection
    85 quaternary epitopes
    86 rate
    87 receptor-binding glycoprotein
    88 respiratory illness
    89 response
    90 roadmap
    91 severe encephalitis
    92 specific antibodies
    93 structure
    94 target
    95 therapeutics
    96 vaccine
    97 virus
    98 schema:name Broadly neutralizing antibody cocktails targeting Nipah virus and Hendra virus fusion glycoproteins
    99 schema:pagination 426-434
    100 schema:productId N2a6974b34f3a443fa633eb21705482bc
    101 N9d156d572e654daea287c7e412900591
    102 Ne3616c7d8ff84df29a5a2614ccec11bf
    103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1137603226
    104 https://doi.org/10.1038/s41594-021-00584-8
    105 schema:sdDatePublished 2022-06-01T22:24
    106 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    107 schema:sdPublisher Ne62e54bfbdcc43b996890549321e6049
    108 schema:url https://doi.org/10.1038/s41594-021-00584-8
    109 sgo:license sg:explorer/license/
    110 sgo:sdDataset articles
    111 rdf:type schema:ScholarlyArticle
    112 N04fcd4d656e349bbbd26d42cc861146a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    113 schema:name Cross Reactions
    114 rdf:type schema:DefinedTerm
    115 N112e303dff5e473ea35db07d206e6ef2 rdf:first sg:person.0705360330.66
    116 rdf:rest Nb3c35912a925414181b3dc0768097e53
    117 N167b7546e6f64efb93c161861b1a7774 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    118 schema:name Viral Fusion Proteins
    119 rdf:type schema:DefinedTerm
    120 N1706a1704d144d9c82000a398fc22702 rdf:first sg:person.013226655707.49
    121 rdf:rest N657a8c855c784ad6a4a7753bba432872
    122 N21fd34d74ec943dab9e3dd7654768d64 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    123 schema:name Humans
    124 rdf:type schema:DefinedTerm
    125 N2a6974b34f3a443fa633eb21705482bc schema:name pubmed_id
    126 schema:value 33927387
    127 rdf:type schema:PropertyValue
    128 N2fc5bb76748d4456a360a94a4d9199eb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    129 schema:name Broadly Neutralizing Antibodies
    130 rdf:type schema:DefinedTerm
    131 N3129fff0dda943a3bca68a6589daef79 rdf:first sg:person.012756216443.47
    132 rdf:rest N47cf2a7348ee44d28aacb5114dd08d39
    133 N3de0f9ae3e034d0ebf91149baba4c2e2 rdf:first sg:person.0630057102.41
    134 rdf:rest N3e3c848995da41efa6dd97abfbe5a695
    135 N3e3c848995da41efa6dd97abfbe5a695 rdf:first sg:person.0606553260.52
    136 rdf:rest N89bf1ca3286c4b6a878af16a29e3f804
    137 N448cd0d714b347cdbc8da82f7b2d2a56 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    138 schema:name Antibodies, Monoclonal, Humanized
    139 rdf:type schema:DefinedTerm
    140 N47cf2a7348ee44d28aacb5114dd08d39 rdf:first sg:person.01321077225.18
    141 rdf:rest N1706a1704d144d9c82000a398fc22702
    142 N47d5b8e1472f4ed9b8241cb39b5120be rdf:first sg:person.014466116617.16
    143 rdf:rest N94ce74dad3294ac4a708f3d63559e360
    144 N4878e7b085dd429c863f89f09c8941ee rdf:first sg:person.01327700701.29
    145 rdf:rest N879ea63e38584aadb9c72325f49dafd7
    146 N4bd006621c444f018053daf603b642df schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    147 schema:name Antibodies, Viral
    148 rdf:type schema:DefinedTerm
    149 N657a8c855c784ad6a4a7753bba432872 rdf:first sg:person.01361432261.18
    150 rdf:rest N6844a3d3e78a4a7095f29cdbf3d55943
    151 N6844a3d3e78a4a7095f29cdbf3d55943 rdf:first sg:person.010005727760.88
    152 rdf:rest Nda494553945442528fe06a95ed86d4b1
    153 N6e8f949ecdde4b229fc4d1cf0bad3027 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    154 schema:name Animals
    155 rdf:type schema:DefinedTerm
    156 N74a65525e7df4e679e9c5a7b3d21127a rdf:first sg:person.01002414777.95
    157 rdf:rest rdf:nil
    158 N77117d493cf54ac58df072eaca86d17c schema:issueNumber 5
    159 rdf:type schema:PublicationIssue
    160 N879ea63e38584aadb9c72325f49dafd7 rdf:first sg:person.0647037406.00
    161 rdf:rest N3de0f9ae3e034d0ebf91149baba4c2e2
    162 N89bf1ca3286c4b6a878af16a29e3f804 rdf:first sg:person.01175422023.99
    163 rdf:rest N47d5b8e1472f4ed9b8241cb39b5120be
    164 N8e6f643f5a11419588521de763da8259 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    165 schema:name HEK293 Cells
    166 rdf:type schema:DefinedTerm
    167 N909803e523d14592bac9f6f682e8e597 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    168 schema:name Virus Internalization
    169 rdf:type schema:DefinedTerm
    170 N92c70dc8643548f8afe6424b80085082 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    171 schema:name Mice
    172 rdf:type schema:DefinedTerm
    173 N94ce74dad3294ac4a708f3d63559e360 rdf:first sg:person.0614131467.47
    174 rdf:rest N74a65525e7df4e679e9c5a7b3d21127a
    175 N9d156d572e654daea287c7e412900591 schema:name doi
    176 schema:value 10.1038/s41594-021-00584-8
    177 rdf:type schema:PropertyValue
    178 Nb1d358e33271456ca026f524d5517a79 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    179 schema:name CHO Cells
    180 rdf:type schema:DefinedTerm
    181 Nb33ae07c38744c3aa4117c12581326e0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    182 schema:name Nipah Virus
    183 rdf:type schema:DefinedTerm
    184 Nb3c35912a925414181b3dc0768097e53 rdf:first sg:person.014561463261.50
    185 rdf:rest N4878e7b085dd429c863f89f09c8941ee
    186 Nb778557f35564364a0ecd7f3c99611a0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    187 schema:name Henipavirus Infections
    188 rdf:type schema:DefinedTerm
    189 Nbaef3fe53bff4e86884d348832b81b77 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    190 schema:name Cricetulus
    191 rdf:type schema:DefinedTerm
    192 Nbeef81381e684078992c22cdcb6510f8 schema:volumeNumber 28
    193 rdf:type schema:PublicationVolume
    194 Nda494553945442528fe06a95ed86d4b1 rdf:first sg:person.01200117555.35
    195 rdf:rest N112e303dff5e473ea35db07d206e6ef2
    196 Ndd65c16011be49968e9da9c9ef7da93d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    197 schema:name Hendra Virus
    198 rdf:type schema:DefinedTerm
    199 Ne3616c7d8ff84df29a5a2614ccec11bf schema:name dimensions_id
    200 schema:value pub.1137603226
    201 rdf:type schema:PropertyValue
    202 Ne62e54bfbdcc43b996890549321e6049 schema:name Springer Nature - SN SciGraph project
    203 rdf:type schema:Organization
    204 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
    205 schema:name Medical and Health Sciences
    206 rdf:type schema:DefinedTerm
    207 anzsrc-for:1107 schema:inDefinedTermSet anzsrc-for:
    208 schema:name Immunology
    209 rdf:type schema:DefinedTerm
    210 anzsrc-for:1108 schema:inDefinedTermSet anzsrc-for:
    211 schema:name Medical Microbiology
    212 rdf:type schema:DefinedTerm
    213 sg:grant.2456178 http://pending.schema.org/fundedItem sg:pub.10.1038/s41594-021-00584-8
    214 rdf:type schema:MonetaryGrant
    215 sg:grant.2688023 http://pending.schema.org/fundedItem sg:pub.10.1038/s41594-021-00584-8
    216 rdf:type schema:MonetaryGrant
    217 sg:grant.2704770 http://pending.schema.org/fundedItem sg:pub.10.1038/s41594-021-00584-8
    218 rdf:type schema:MonetaryGrant
    219 sg:grant.5503638 http://pending.schema.org/fundedItem sg:pub.10.1038/s41594-021-00584-8
    220 rdf:type schema:MonetaryGrant
    221 sg:grant.6803473 http://pending.schema.org/fundedItem sg:pub.10.1038/s41594-021-00584-8
    222 rdf:type schema:MonetaryGrant
    223 sg:grant.7169995 http://pending.schema.org/fundedItem sg:pub.10.1038/s41594-021-00584-8
    224 rdf:type schema:MonetaryGrant
    225 sg:grant.8387643 http://pending.schema.org/fundedItem sg:pub.10.1038/s41594-021-00584-8
    226 rdf:type schema:MonetaryGrant
    227 sg:grant.9534495 http://pending.schema.org/fundedItem sg:pub.10.1038/s41594-021-00584-8
    228 rdf:type schema:MonetaryGrant
    229 sg:journal.1295033 schema:issn 1545-9993
    230 2331-365X
    231 schema:name Nature Structural & Molecular Biology
    232 schema:publisher Springer Nature
    233 rdf:type schema:Periodical
    234 sg:person.010005727760.88 schema:affiliation grid-institutes:grid.421122.6
    235 schema:familyName West
    236 schema:givenName Brandyn R.
    237 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010005727760.88
    238 rdf:type schema:Person
    239 sg:person.01002414777.95 schema:affiliation grid-institutes:grid.34477.33
    240 schema:familyName Veesler
    241 schema:givenName David
    242 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01002414777.95
    243 rdf:type schema:Person
    244 sg:person.01175422023.99 schema:affiliation grid-institutes:grid.421122.6
    245 schema:familyName Zeitlin
    246 schema:givenName Larry
    247 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01175422023.99
    248 rdf:type schema:Person
    249 sg:person.01200117555.35 schema:affiliation grid-institutes:grid.265436.0
    250 schema:familyName Chan
    251 schema:givenName Yee-Peng
    252 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01200117555.35
    253 rdf:type schema:Person
    254 sg:person.012756216443.47 schema:affiliation grid-institutes:grid.34477.33
    255 schema:familyName Dang
    256 schema:givenName Ha V.
    257 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012756216443.47
    258 rdf:type schema:Person
    259 sg:person.01321077225.18 schema:affiliation grid-institutes:grid.176731.5
    260 schema:familyName Cross
    261 schema:givenName Robert W.
    262 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01321077225.18
    263 rdf:type schema:Person
    264 sg:person.013226655707.49 schema:affiliation grid-institutes:grid.176731.5
    265 schema:familyName Borisevich
    266 schema:givenName Viktoriya
    267 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013226655707.49
    268 rdf:type schema:Person
    269 sg:person.01327700701.29 schema:affiliation grid-institutes:grid.265436.0
    270 schema:familyName Dimitrov
    271 schema:givenName Antony S.
    272 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01327700701.29
    273 rdf:type schema:Person
    274 sg:person.01361432261.18 schema:affiliation grid-institutes:grid.421122.6
    275 schema:familyName Bornholdt
    276 schema:givenName Zachary A.
    277 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01361432261.18
    278 rdf:type schema:Person
    279 sg:person.014466116617.16 schema:affiliation grid-institutes:grid.176731.5
    280 schema:familyName Geisbert
    281 schema:givenName Thomas W.
    282 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014466116617.16
    283 rdf:type schema:Person
    284 sg:person.014561463261.50 schema:affiliation grid-institutes:grid.265436.0
    285 schema:familyName Da Silva
    286 schema:givenName Sofia Cheliout
    287 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014561463261.50
    288 rdf:type schema:Person
    289 sg:person.0606553260.52 schema:affiliation grid-institutes:grid.66875.3a
    290 schema:familyName Navaratnarajah
    291 schema:givenName Chanakha K.
    292 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0606553260.52
    293 rdf:type schema:Person
    294 sg:person.0614131467.47 schema:affiliation grid-institutes:grid.265436.0
    295 schema:familyName Broder
    296 schema:givenName Christopher C.
    297 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0614131467.47
    298 rdf:type schema:Person
    299 sg:person.0630057102.41 schema:affiliation grid-institutes:grid.265436.0
    300 schema:familyName Amaya
    301 schema:givenName Moushimi
    302 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0630057102.41
    303 rdf:type schema:Person
    304 sg:person.0647037406.00 schema:affiliation grid-institutes:grid.265436.0
    305 schema:familyName Yan
    306 schema:givenName Lianying
    307 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0647037406.00
    308 rdf:type schema:Person
    309 sg:person.0705360330.66 schema:affiliation grid-institutes:grid.176731.5
    310 schema:familyName Mire
    311 schema:givenName Chad E.
    312 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0705360330.66
    313 rdf:type schema:Person
    314 sg:pub.10.1007/82_2012_207 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007852348
    315 https://doi.org/10.1007/82_2012_207
    316 rdf:type schema:CreativeWork
    317 sg:pub.10.1007/978-3-540-72146-8_3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015864377
    318 https://doi.org/10.1007/978-3-540-72146-8_3
    319 rdf:type schema:CreativeWork
    320 sg:pub.10.1038/nature03838 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031943292
    321 https://doi.org/10.1038/nature03838
    322 rdf:type schema:CreativeWork
    323 sg:pub.10.1038/ncomms1796 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043718269
    324 https://doi.org/10.1038/ncomms1796
    325 rdf:type schema:CreativeWork
    326 sg:pub.10.1038/ncomms6342 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028480583
    327 https://doi.org/10.1038/ncomms6342
    328 rdf:type schema:CreativeWork
    329 sg:pub.10.1038/nmeth.2115 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041024070
    330 https://doi.org/10.1038/nmeth.2115
    331 rdf:type schema:CreativeWork
    332 sg:pub.10.1038/nmeth.3286 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015110469
    333 https://doi.org/10.1038/nmeth.3286
    334 rdf:type schema:CreativeWork
    335 sg:pub.10.1038/nmeth.3541 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004871597
    336 https://doi.org/10.1038/nmeth.3541
    337 rdf:type schema:CreativeWork
    338 sg:pub.10.1038/nmeth.4169 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083686092
    339 https://doi.org/10.1038/nmeth.4169
    340 rdf:type schema:CreativeWork
    341 sg:pub.10.1038/nmeth.4193 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084129286
    342 https://doi.org/10.1038/nmeth.4193
    343 rdf:type schema:CreativeWork
    344 sg:pub.10.1038/nrmicro1323 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021039329
    345 https://doi.org/10.1038/nrmicro1323
    346 rdf:type schema:CreativeWork
    347 sg:pub.10.1038/nsmb.1435 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044123311
    348 https://doi.org/10.1038/nsmb.1435
    349 rdf:type schema:CreativeWork
    350 sg:pub.10.1038/nsmb.3115 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010755414
    351 https://doi.org/10.1038/nsmb.3115
    352 rdf:type schema:CreativeWork
    353 sg:pub.10.1038/s41467-017-00863-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1092015759
    354 https://doi.org/10.1038/s41467-017-00863-3
    355 rdf:type schema:CreativeWork
    356 sg:pub.10.1038/s41586-020-2349-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1127684942
    357 https://doi.org/10.1038/s41586-020-2349-y
    358 rdf:type schema:CreativeWork
    359 sg:pub.10.1038/s41592-020-00990-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1132982567
    360 https://doi.org/10.1038/s41592-020-00990-8
    361 rdf:type schema:CreativeWork
    362 sg:pub.10.1038/s41594-019-0308-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1121383201
    363 https://doi.org/10.1038/s41594-019-0308-9
    364 rdf:type schema:CreativeWork
    365 sg:pub.10.1186/s12985-018-0964-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1101776899
    366 https://doi.org/10.1186/s12985-018-0964-0
    367 rdf:type schema:CreativeWork
    368 grid-institutes:grid.176731.5 schema:alternateName Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA
    369 Galveston National Laboratory, University of Texas Medical Branch, Galveston, TX, USA
    370 schema:name Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA
    371 Galveston National Laboratory, University of Texas Medical Branch, Galveston, TX, USA
    372 rdf:type schema:Organization
    373 grid-institutes:grid.265436.0 schema:alternateName Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA
    374 schema:name Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA
    375 rdf:type schema:Organization
    376 grid-institutes:grid.34477.33 schema:alternateName Department of Biochemistry, University of Washington, Seattle, WA, USA
    377 schema:name Department of Biochemistry, University of Washington, Seattle, WA, USA
    378 rdf:type schema:Organization
    379 grid-institutes:grid.421122.6 schema:alternateName Mapp Biopharmaceutical, Inc., San Diego, CA, USA
    380 schema:name Mapp Biopharmaceutical, Inc., San Diego, CA, USA
    381 rdf:type schema:Organization
    382 grid-institutes:grid.66875.3a schema:alternateName Department of Molecular Medicine, Mayo Clinic, Rochester, MN, USA
    383 schema:name Department of Molecular Medicine, Mayo Clinic, Rochester, MN, USA
    384 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...