Structural basis for human coronavirus attachment to sialic acid receptors View Full Text


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Article Info

DATE

2019-06-03

AUTHORS

M. Alejandra Tortorici, Alexandra C. Walls, Yifei Lang, Chunyan Wang, Zeshi Li, Danielle Koerhuis, Geert-Jan Boons, Berend-Jan Bosch, Félix A. Rey, Raoul J. de Groot, David Veesler

ABSTRACT

Coronaviruses cause respiratory tract infections in humans and outbreaks of deadly pneumonia worldwide. Infections are initiated by the transmembrane spike (S) glycoprotein, which binds to host receptors and fuses the viral and cellular membranes. To understand the molecular basis of coronavirus attachment to oligosaccharide receptors, we determined cryo-EM structures of coronavirus OC43 S glycoprotein trimer in isolation and in complex with a 9-O-acetylated sialic acid. We show that the ligand binds with fast kinetics to a surface-exposed groove and that interactions at the identified site are essential for S-mediated viral entry into host cells, but free monosaccharide does not trigger fusogenic conformational changes. The receptor-interacting site is conserved in all coronavirus S glycoproteins that engage 9-O-acetyl-sialogycans, with an architecture similar to those of the ligand-binding pockets of coronavirus hemagglutinin esterases and influenza virus C/D hemagglutinin-esterase fusion glycoproteins. Our results demonstrate these viruses evolved similar strategies to engage sialoglycans at the surface of target cells. More... »

PAGES

481-489

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41594-019-0233-y

    DOI

    http://dx.doi.org/10.1038/s41594-019-0233-y

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1116577957

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/31160783


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    361 grid-institutes:grid.428999.7 schema:alternateName CNRS UMR 3569, Unité de Virologie Structurale, Paris, France
    362 schema:name CNRS UMR 3569, Unité de Virologie Structurale, Paris, France
    363 Department of Biochemistry, University of Washington, Seattle, WA USA
    364 Institut Pasteur, Unité de Virologie Structurale, Paris, France
    365 rdf:type schema:Organization
    366 grid-institutes:grid.5477.1 schema:alternateName Bijvoet Center for Biomolecular Research, Utrecht University, Utrecht, the Netherlands
    367 Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands
    368 schema:name Bijvoet Center for Biomolecular Research, Utrecht University, Utrecht, the Netherlands
    369 Department of Chemical Biology and Drug Discovery, Utrecht University, Utrecht, the Netherlands
    370 Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands
    371 rdf:type schema:Organization
     




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