Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2018-08

AUTHORS

Francesca Pennacchietti, Ekaterina O. Serebrovskaya, Aline R. Faro, Irina I. Shemyakina, Nina G. Bozhanova, Alexey A. Kotlobay, Nadya G. Gurskaya, Andreas Bodén, Jes Dreier, Dmitry M. Chudakov, Konstantin A. Lukyanov, Vladislav V. Verkhusha, Alexander S. Mishin, Ilaria Testa

ABSTRACT

Reversibly photoswitchable fluorescent proteins (rsFPs) are gaining popularity as tags for optical nanoscopy because they make it possible to image with lower light doses. However, green rsFPs need violet-blue light for photoswitching, which is potentially phototoxic and highly scattering. We developed new rsFPs based on FusionRed that are reversibly photoswitchable with green-orange light. The rsFusionReds are bright and exhibit rapid photoswitching, thereby enabling nanoscale imaging of living cells. More... »

PAGES

601-604

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41592-018-0052-9

DOI

http://dx.doi.org/10.1038/s41592-018-0052-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1105275285

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29988095


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