Single-cell profiling of proteins and chromatin accessibility using PHAGE-ATAC View Full Text


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Article Info

DATE

2021-10-21

AUTHORS

Evgenij Fiskin, Caleb A. Lareau, Leif S. Ludwig, Gökcen Eraslan, Feimei Liu, Aaron M. Ring, Ramnik J. Xavier, Aviv Regev

ABSTRACT

Multimodal measurements of single-cell profiles are proving increasingly useful for characterizing cell states and regulatory mechanisms. In the present study, we developed PHAGE-ATAC (Assay for Transposase-Accessible Chromatin), a massively parallel droplet-based method that uses phage displaying, engineered, camelid single-domain antibodies (‘nanobodies’) for simultaneous single-cell measurements of protein levels and chromatin accessibility profiles, and mitochondrial DNA-based clonal tracing. We use PHAGE-ATAC for multimodal analysis in primary human immune cells, sample multiplexing, intracellular protein analysis and the detection of SARS-CoV-2 spike protein in human cell populations. Finally, we construct a synthetic high-complexity phage library for selection of antigen-specific nanobodies that bind cells of particular molecular profiles, opening an avenue for protein detection, cell characterization and screening with single-cell genomics. More... »

PAGES

374-381

References to SciGraph publications

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41587-021-01065-5

    DOI

    http://dx.doi.org/10.1038/s41587-021-01065-5

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/34675424


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    52 chromatin accessibility profiles
    53 clonal tracing
    54 detection
    55 displaying
    56 droplet-based method
    57 genomics
    58 human cell populations
    59 human immune cells
    60 immune cells
    61 intracellular protein analysis
    62 levels
    63 library
    64 measurements
    65 mechanism
    66 method
    67 mitochondrial DNA
    68 molecular profile
    69 multimodal analysis
    70 multimodal measurements
    71 multiplexing
    72 nanobodies
    73 particular molecular profiles
    74 phage library
    75 population
    76 present study
    77 primary human immune cells
    78 profile
    79 profiling
    80 protein
    81 protein analysis
    82 protein detection
    83 protein levels
    84 regulatory mechanisms
    85 sample multiplexing
    86 selection
    87 simultaneous single-cell measurements
    88 single-cell genomics
    89 single-cell measurements
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    91 single-cell profiling
    92 single-domain antibodies
    93 spike protein
    94 state
    95 study
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