Evaluating drug targets through human loss-of-function genetic variation View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2020-05-27

AUTHORS

Eric Vallabh Minikel, Konrad J. Karczewski, Hilary C. Martin, Beryl B. Cummings, Nicola Whiffin, Daniel Rhodes, Jessica Alföldi, Richard C. Trembath, David A. van Heel, Mark J. Daly, Stuart L. Schreiber, Daniel G. MacArthur

ABSTRACT

Naturally occurring human genetic variants that are predicted to inactivate protein-coding genes provide an in vivo model of human gene inactivation that complements knockout studies in cells and model organisms. Here we report three key findings regarding the assessment of candidate drug targets using human loss-of-function variants. First, even essential genes, in which loss-of-function variants are not tolerated, can be highly successful as targets of inhibitory drugs. Second, in most genes, loss-of-function variants are sufficiently rare that genotype-based ascertainment of homozygous or compound heterozygous ‘knockout’ humans will await sample sizes that are approximately 1,000 times those presently available, unless recruitment focuses on consanguineous individuals. Third, automated variant annotation and filtering are powerful, but manual curation remains crucial for removing artefacts, and is a prerequisite for recall-by-genotype efforts. Our results provide a roadmap for human knockout studies and should guide the interpretation of loss-of-function variants in drug development. More... »

PAGES

459-464

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  • Journal

    TITLE

    Nature

    ISSUE

    7809

    VOLUME

    581

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41586-020-2267-z

    DOI

    http://dx.doi.org/10.1038/s41586-020-2267-z

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1127893062

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/32461653


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