Gut bacteria responding to dietary change encode sialidases that exhibit preference for red meat-associated carbohydrates View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-09-23

AUTHORS

Livia S. Zaramela, Cameron Martino, Frederico Alisson-Silva, Steven D. Rees, Sandra L. Diaz, Léa Chuzel, Mehul B. Ganatra, Christopher H. Taron, Patrick Secrest, Cristal Zuñiga, Jianbo Huang, Dionicio Siegel, Geoffrey Chang, Ajit Varki, Karsten Zengler

ABSTRACT

Dietary habits have been associated with alterations of the human gut resident microorganisms contributing to obesity, diabetes and cancer1. In Western diets, red meat is a frequently eaten food2, but long-term consumption has been associated with increased risk of disease3,4. Red meat is enriched in N-glycolylneuraminic acid (Neu5Gc) that cannot be synthesized by humans5. However, consumption can cause Neu5Gc incorporation into cell surface glycans6, especially in carcinomas4,7. As a consequence, an inflammatory response is triggered when Neu5Gc-containing glycans encounter circulating anti-Neu5Gc antibodies8,9. Although bacteria can use free sialic acids as a nutrient source10–12, it is currently unknown if gut microorganisms contribute to releasing Neu5Gc from food. We found that a Neu5Gc-rich diet induces changes in the gut microbiota, with Bacteroidales and Clostridiales responding the most. Genome assembling of mouse and human shotgun metagenomic sequencing identified bacterial sialidases with previously unobserved substrate preference for Neu5Gc-containing glycans. X-ray crystallography revealed key amino acids potentially contributing to substrate preference. Additionally, we verified that mouse and human sialidases were able to release Neu5Gc from red meat. The release of Neu5Gc from red meat using bacterial sialidases could reduce the risk of inflammatory diseases associated with red meat consumption, including colorectal cancer4 and atherosclerosis13. More... »

PAGES

2082-2089

References to SciGraph publications

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41564-019-0564-9

    DOI

    http://dx.doi.org/10.1038/s41564-019-0564-9

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1121185303

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/31548686


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