Molecular adaptations of NADP-malic enzyme for its function in C4 photosynthesis in grasses View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2019-06-24

AUTHORS

Clarisa E. Alvarez, Anastasiia Bovdilova, Astrid Höppner, Christian-Claus Wolff, Mariana Saigo, Felipe Trajtenberg, Tao Zhang, Alejandro Buschiazzo, Luitgard Nagel-Steger, Maria F. Drincovich, Martin J. Lercher, Veronica G. Maurino

ABSTRACT

In C4 grasses of agronomical interest, malate shuttled into the bundle sheath cells is decarboxylated mainly by nicotinamide adenine dinucleotide phosphate (NADP)-malic enzyme (C4-NADP-ME). The activity of C4-NADP-ME was optimized by natural selection to efficiently deliver CO2 to Rubisco. During its evolution from a plastidic non-photosynthetic NADP-ME, C4-NADP-ME acquired increased catalytic efficiency, tetrameric structure and pH-dependent inhibition by its substrate malate. Here, we identified specific amino acids important for these C4 adaptions based on strict differential conservation of amino acids, combined with solving the crystal structures of maize and sorghum C4-NADP-ME. Site-directed mutagenesis and structural analyses show that Q503, L544 and E339 are involved in catalytic efficiency; E339 confers pH-dependent regulation by malate, F140 is critical for the stabilization of the oligomeric structure and the N-terminal region is involved in tetramerization. Together, the identified molecular adaptations form the basis for the efficient catalysis and regulation of one of the central biochemical steps in C4 metabolism. More... »

PAGES

755-765

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41477-019-0451-7

DOI

http://dx.doi.org/10.1038/s41477-019-0451-7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1117482314

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/31235877


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57 differential conservation
58 dinucleotide
59 efficiency
60 efficient catalysis
61 enzyme
62 evolution
63 function
64 grass
65 inhibition
66 interest
67 maize
68 malate
69 malic enzyme
70 metabolism
71 molecular adaptations
72 mutagenesis
73 natural selection
74 nicotinamide adenine dinucleotide
75 oligomeric structure
76 pH-dependent inhibition
77 pH-dependent regulation
78 photosynthesis
79 region
80 regulation
81 selection
82 sheath cells
83 site-directed mutagenesis
84 specific amino acids
85 stabilization
86 step
87 structural analysis
88 structure
89 substrate malate
90 tetrameric structure
91 tetramerization
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