Cumulative SARS-CoV-2 mutations and corresponding changes in immunity in an immunocompromised patient indicate viral evolution within the host View Full Text


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Article Info

DATE

2022-05-10

AUTHORS

Sissy Therese Sonnleitner, Martina Prelog, Stefanie Sonnleitner, Eva Hinterbichler, Hannah Halbfurter, Dominik B. C. Kopecky, Giovanni Almanzar, Stephan Koblmüller, Christian Sturmbauer, Leonard Feist, Ralf Horres, Wilfried Posch, Gernot Walder

ABSTRACT

Different scenarios explaining the emergence of novel variants of concern (VOC) of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been reported, including their evolution in scarcely monitored populations, in animals as alternative hosts, or in immunocompromised individuals. Here we report SARS-CoV-2 immune escape mutations over a period of seven months in an immunocompromised patient with prolonged viral shedding. Signs of infection, viral shedding and mutation events are periodically analyzed using RT-PCR and next-generation sequencing based on naso-pharyngeal swabs, with the results complemented by immunological diagnostics to determine humoral and T cell immune responses. Throughout the infection course, 17 non-synonymous intra-host mutations are noted, with 15 (88.2%) having been previously described as prominent immune escape mutations (S:E484K, S:D950N, S:P681H, S:N501Y, S:del(9), N:S235F and S:H655Y) in VOCs. The high frequency of these non-synonymous mutations is consistent with multiple events of convergent evolution. Thus, our results suggest that specific mutations in the SARS-CoV-2 genome may represent positions with a fitness advantage, and may serve as targets in future vaccine and therapeutics development for COVID-19. More... »

PAGES

2560

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41467-022-30163-4

    DOI

    http://dx.doi.org/10.1038/s41467-022-30163-4

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1147767484

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/35538074


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    336 rdf:type schema:Organization
    337 grid-institutes:grid.5361.1 schema:alternateName Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck, 6020, Innsbruck, Austria
    338 schema:name Infektiologie Tirol, Department of Virology, Unterwalden 30, 9931, Außervillgraten, Austria
    339 Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck, 6020, Innsbruck, Austria
    340 rdf:type schema:Organization
     




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