Ontology type: schema:ScholarlyArticle Open Access: True
2019-10-04
AUTHORSMarcela Garita-Hernandez, Maruša Lampič, Antoine Chaffiol, Laure Guibbal, Fiona Routet, Tiago Santos-Ferreira, Sylvia Gasparini, Oliver Borsch, Giuliana Gagliardi, Sacha Reichman, Serge Picaud, José-Alain Sahel, Olivier Goureau, Marius Ader, Deniz Dalkara, Jens Duebel
ABSTRACTA major challenge in the treatment of retinal degenerative diseases, with the transplantation of replacement photoreceptors, is the difficulty in inducing the grafted cells to grow and maintain light sensitive outer segments in the host retina, which depends on proper interaction with the underlying retinal pigment epithelium (RPE). Here, for an RPE-independent treatment approach, we introduce a hyperpolarizing microbial opsin into photoreceptor precursors from newborn mice, and transplant them into blind mice lacking the photoreceptor layer. These optogenetically-transformed photoreceptors are light responsive and their transplantation leads to the recovery of visual function, as shown by ganglion cell recordings and behavioral tests. Subsequently, we generate cone photoreceptors from human induced pluripotent stem cells, expressing the chloride pump Jaws. After transplantation into blind mice, we observe light-driven responses at the photoreceptor and ganglion cell levels. These results demonstrate that structural and functional retinal repair is possible by combining stem cell therapy and optogenetics. More... »
PAGES4524
http://scigraph.springernature.com/pub.10.1038/s41467-019-12330-2
DOIhttp://dx.doi.org/10.1038/s41467-019-12330-2
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1121474706
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/31586094
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Medical and Health Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1109",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Neurosciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1113",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Ophthalmology and Optometry",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Animals",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Animals, Newborn",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cell Culture Techniques",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cell Engineering",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Dependovirus",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Disease Models, Animal",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Female",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Genetic Vectors",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "HEK293 Cells",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Halorhodopsins",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Humans",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Induced Pluripotent Stem Cells",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Male",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mice",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mice, Knockout",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Optogenetics",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Photoreceptor Cells, Vertebrate",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Retinal Degeneration",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Rhodopsin",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Transfection",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Treatment Outcome",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Sorbonne Universit\u00e9, Institut de la Vision, INSERM, CNRS, 75012, Paris, France",
"id": "http://www.grid.ac/institutes/None",
"name": [
"Sorbonne Universit\u00e9, Institut de la Vision, INSERM, CNRS, 75012, Paris, France"
],
"type": "Organization"
},
"familyName": "Garita-Hernandez",
"givenName": "Marcela",
"id": "sg:person.01001210443.52",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01001210443.52"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Sorbonne Universit\u00e9, Institut de la Vision, INSERM, CNRS, 75012, Paris, France",
"id": "http://www.grid.ac/institutes/None",
"name": [
"Sorbonne Universit\u00e9, Institut de la Vision, INSERM, CNRS, 75012, Paris, France"
],
"type": "Organization"
},
"familyName": "Lampi\u010d",
"givenName": "Maru\u0161a",
"id": "sg:person.016061735035.45",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016061735035.45"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Sorbonne Universit\u00e9, Institut de la Vision, INSERM, CNRS, 75012, Paris, France",
"id": "http://www.grid.ac/institutes/None",
"name": [
"Sorbonne Universit\u00e9, Institut de la Vision, INSERM, CNRS, 75012, Paris, France"
],
"type": "Organization"
},
"familyName": "Chaffiol",
"givenName": "Antoine",
"id": "sg:person.0673302301.86",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0673302301.86"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Sorbonne Universit\u00e9, Institut de la Vision, INSERM, CNRS, 75012, Paris, France",
"id": "http://www.grid.ac/institutes/None",
"name": [
"Sorbonne Universit\u00e9, Institut de la Vision, INSERM, CNRS, 75012, Paris, France"
],
"type": "Organization"
},
"familyName": "Guibbal",
"givenName": "Laure",
"id": "sg:person.01177660356.64",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01177660356.64"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Sorbonne Universit\u00e9, Institut de la Vision, INSERM, CNRS, 75012, Paris, France",
"id": "http://www.grid.ac/institutes/None",
"name": [
"Sorbonne Universit\u00e9, Institut de la Vision, INSERM, CNRS, 75012, Paris, France"
],
"type": "Organization"
},
"familyName": "Routet",
"givenName": "Fiona",
"id": "sg:person.01175674217.51",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01175674217.51"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "CRTD/Center for Regenerative Therapies Dresden, CMCB, TU Dresden, Dresden, Germany",
"id": "http://www.grid.ac/institutes/grid.4488.0",
"name": [
"CRTD/Center for Regenerative Therapies Dresden, CMCB, TU Dresden, Dresden, Germany"
],
"type": "Organization"
},
"familyName": "Santos-Ferreira",
"givenName": "Tiago",
"id": "sg:person.0740541211.62",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0740541211.62"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "CRTD/Center for Regenerative Therapies Dresden, CMCB, TU Dresden, Dresden, Germany",
"id": "http://www.grid.ac/institutes/grid.4488.0",
"name": [
"CRTD/Center for Regenerative Therapies Dresden, CMCB, TU Dresden, Dresden, Germany"
],
"type": "Organization"
},
"familyName": "Gasparini",
"givenName": "Sylvia",
"id": "sg:person.0777770576.87",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0777770576.87"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "CRTD/Center for Regenerative Therapies Dresden, CMCB, TU Dresden, Dresden, Germany",
"id": "http://www.grid.ac/institutes/grid.4488.0",
"name": [
"CRTD/Center for Regenerative Therapies Dresden, CMCB, TU Dresden, Dresden, Germany"
],
"type": "Organization"
},
"familyName": "Borsch",
"givenName": "Oliver",
"id": "sg:person.012622601161.79",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012622601161.79"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Sorbonne Universit\u00e9, Institut de la Vision, INSERM, CNRS, 75012, Paris, France",
"id": "http://www.grid.ac/institutes/None",
"name": [
"Sorbonne Universit\u00e9, Institut de la Vision, INSERM, CNRS, 75012, Paris, France"
],
"type": "Organization"
},
"familyName": "Gagliardi",
"givenName": "Giuliana",
"id": "sg:person.016133773347.21",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016133773347.21"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Sorbonne Universit\u00e9, Institut de la Vision, INSERM, CNRS, 75012, Paris, France",
"id": "http://www.grid.ac/institutes/None",
"name": [
"Sorbonne Universit\u00e9, Institut de la Vision, INSERM, CNRS, 75012, Paris, France"
],
"type": "Organization"
},
"familyName": "Reichman",
"givenName": "Sacha",
"id": "sg:person.0600655241.05",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0600655241.05"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Sorbonne Universit\u00e9, Institut de la Vision, INSERM, CNRS, 75012, Paris, France",
"id": "http://www.grid.ac/institutes/None",
"name": [
"Sorbonne Universit\u00e9, Institut de la Vision, INSERM, CNRS, 75012, Paris, France"
],
"type": "Organization"
},
"familyName": "Picaud",
"givenName": "Serge",
"id": "sg:person.0642273724.04",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0642273724.04"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Ophthalmology, The University of Pittsburgh School of Medicine, Pittsburgh, USA",
"id": "http://www.grid.ac/institutes/grid.21925.3d",
"name": [
"Sorbonne Universit\u00e9, Institut de la Vision, INSERM, CNRS, 75012, Paris, France",
"CHNO des Quinze\u2212Vingts, DHU Sight Restore, INSERM-DGOS CIC 1423, Paris, France",
"Department of Ophthalmology, The University of Pittsburgh School of Medicine, Pittsburgh, USA"
],
"type": "Organization"
},
"familyName": "Sahel",
"givenName": "Jos\u00e9-Alain",
"id": "sg:person.01104041722.66",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01104041722.66"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Sorbonne Universit\u00e9, Institut de la Vision, INSERM, CNRS, 75012, Paris, France",
"id": "http://www.grid.ac/institutes/None",
"name": [
"Sorbonne Universit\u00e9, Institut de la Vision, INSERM, CNRS, 75012, Paris, France"
],
"type": "Organization"
},
"familyName": "Goureau",
"givenName": "Olivier",
"id": "sg:person.0776412526.00",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0776412526.00"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "CRTD/Center for Regenerative Therapies Dresden, CMCB, TU Dresden, Dresden, Germany",
"id": "http://www.grid.ac/institutes/grid.4488.0",
"name": [
"CRTD/Center for Regenerative Therapies Dresden, CMCB, TU Dresden, Dresden, Germany"
],
"type": "Organization"
},
"familyName": "Ader",
"givenName": "Marius",
"id": "sg:person.01146111335.04",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01146111335.04"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Sorbonne Universit\u00e9, Institut de la Vision, INSERM, CNRS, 75012, Paris, France",
"id": "http://www.grid.ac/institutes/None",
"name": [
"Sorbonne Universit\u00e9, Institut de la Vision, INSERM, CNRS, 75012, Paris, France"
],
"type": "Organization"
},
"familyName": "Dalkara",
"givenName": "Deniz",
"id": "sg:person.01131140130.98",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01131140130.98"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Ophthalmology, University Medical Center G\u00f6ttingen, G\u00f6ttingen, Germany",
"id": "http://www.grid.ac/institutes/grid.411984.1",
"name": [
"Sorbonne Universit\u00e9, Institut de la Vision, INSERM, CNRS, 75012, Paris, France",
"Department of Ophthalmology, University Medical Center G\u00f6ttingen, G\u00f6ttingen, Germany"
],
"type": "Organization"
},
"familyName": "Duebel",
"givenName": "Jens",
"id": "sg:person.01156573604.98",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01156573604.98"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1038/ncomms13537",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1003928825",
"https://doi.org/10.1038/ncomms13537"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/srep29784",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045026906",
"https://doi.org/10.1038/srep29784"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/srep30742",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1005340457",
"https://doi.org/10.1038/srep30742"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/ncomms13029",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1043362143",
"https://doi.org/10.1038/ncomms13029"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nn.3752",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045545623",
"https://doi.org/10.1038/nn.3752"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/ng0297-216",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1013571435",
"https://doi.org/10.1038/ng0297-216"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/ncomms13028",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1031815807",
"https://doi.org/10.1038/ncomms13028"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/ncomms5047",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1034957544",
"https://doi.org/10.1038/ncomms5047"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nrg2717",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026483567",
"https://doi.org/10.1038/nrg2717"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s11068-008-9027-6",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009069983",
"https://doi.org/10.1007/s11068-008-9027-6"
],
"type": "CreativeWork"
}
],
"datePublished": "2019-10-04",
"datePublishedReg": "2019-10-04",
"description": "A major challenge in the treatment of retinal degenerative diseases, with the transplantation of replacement photoreceptors, is the difficulty in inducing the grafted cells to grow and maintain light sensitive outer segments in the host retina, which depends on proper interaction with the underlying retinal pigment epithelium (RPE). Here, for an RPE-independent treatment approach, we introduce a hyperpolarizing microbial opsin into photoreceptor precursors from newborn mice, and transplant them into blind mice lacking the photoreceptor layer. These optogenetically-transformed photoreceptors are light responsive and their transplantation leads to the recovery of visual function, as shown by ganglion cell recordings and behavioral tests. Subsequently, we generate cone photoreceptors from human induced pluripotent stem cells, expressing the chloride pump Jaws. After transplantation into blind mice, we observe light-driven responses at the photoreceptor and ganglion cell levels. These results demonstrate that structural and functional retinal repair is possible by combining stem cell therapy and optogenetics.",
"genre": "article",
"id": "sg:pub.10.1038/s41467-019-12330-2",
"inLanguage": "en",
"isAccessibleForFree": true,
"isFundedItemOf": [
{
"id": "sg:grant.3799017",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.5051200",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.3799836",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.7936611",
"type": "MonetaryGrant"
}
],
"isPartOf": [
{
"id": "sg:journal.1043282",
"issn": [
"2041-1723"
],
"name": "Nature Communications",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "1",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "10"
}
],
"keywords": [
"retinal pigment epithelium",
"visual function",
"blind mice",
"ganglion cell level",
"light-sensitive outer segments",
"retinal degenerative diseases",
"stem cell therapy",
"ganglion cell recordings",
"host retina",
"photoreceptor layer",
"newborn mice",
"pigment epithelium",
"treatment approaches",
"retinal repair",
"transplantation",
"cell recordings",
"behavioral tests",
"cone photoreceptors",
"cell therapy",
"degenerative diseases",
"human induced pluripotent stem cells",
"photoreceptor precursors",
"mice",
"induced pluripotent stem cells",
"outer segments",
"stem cells",
"light-driven responses",
"pluripotent stem cells",
"photoreceptors",
"microbial opsins",
"cell level",
"cells",
"therapy",
"disease",
"retina",
"epithelium",
"treatment",
"jaw",
"repair",
"optogenetics",
"recordings",
"response",
"function",
"levels",
"opsin",
"recovery",
"restoration",
"major challenge",
"test",
"segments",
"proper interaction",
"difficulties",
"results",
"challenges",
"precursors",
"interaction",
"approach",
"layer"
],
"name": "Restoration of visual function by transplantation of optogenetically engineered photoreceptors",
"pagination": "4524",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1121474706"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1038/s41467-019-12330-2"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"31586094"
]
}
],
"sameAs": [
"https://doi.org/10.1038/s41467-019-12330-2",
"https://app.dimensions.ai/details/publication/pub.1121474706"
],
"sdDataset": "articles",
"sdDatePublished": "2022-05-20T07:35",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220519/entities/gbq_results/article/article_812.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1038/s41467-019-12330-2"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41467-019-12330-2'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41467-019-12330-2'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41467-019-12330-2'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41467-019-12330-2'
This table displays all metadata directly associated to this object as RDF triples.
372 TRIPLES
22 PREDICATES
116 URIs
97 LITERALS
28 BLANK NODES