Automating multimodal microscopy with NanoJ-Fluidics View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Pedro Almada, Pedro M. Pereira, Siân Culley, Ghislaine Caillol, Fanny Boroni-Rueda, Christina L. Dix, Guillaume Charras, Buzz Baum, Romain F. Laine, Christophe Leterrier, Ricardo Henriques

ABSTRACT

Combining and multiplexing microscopy approaches is crucial to understand cellular events, but requires elaborate workflows. Here, we present a robust, open-source approach for treating, labelling and imaging live or fixed cells in automated sequences. NanoJ-Fluidics is based on low-cost Lego hardware controlled by ImageJ-based software, making high-content, multimodal imaging easy to implement on any microscope with high reproducibility. We demonstrate its capacity on event-driven, super-resolved live-to-fixed and multiplexed STORM/DNA-PAINT experiments. More... »

PAGES

1223

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41467-019-09231-9

DOI

http://dx.doi.org/10.1038/s41467-019-09231-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1112759994

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30874553


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0801", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Artificial Intelligence and Image Processing", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/08", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Information and Computing Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University College London", 
          "id": "https://www.grid.ac/institutes/grid.83440.3b", 
          "name": [
            "MRC-Laboratory for Molecular Cell Biology, University College London, WC1E 6BT, London, UK", 
            "Department of Cell and Developmental Biology, University College London, WC1E 6BT, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Almada", 
        "givenName": "Pedro", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Francis Crick Institute", 
          "id": "https://www.grid.ac/institutes/grid.451388.3", 
          "name": [
            "MRC-Laboratory for Molecular Cell Biology, University College London, WC1E 6BT, London, UK", 
            "Department of Cell and Developmental Biology, University College London, WC1E 6BT, London, UK", 
            "The Francis Crick Institute, NW1 1AT, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pereira", 
        "givenName": "Pedro M.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Francis Crick Institute", 
          "id": "https://www.grid.ac/institutes/grid.451388.3", 
          "name": [
            "MRC-Laboratory for Molecular Cell Biology, University College London, WC1E 6BT, London, UK", 
            "Department of Cell and Developmental Biology, University College London, WC1E 6BT, London, UK", 
            "The Francis Crick Institute, NW1 1AT, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Culley", 
        "givenName": "Sia\u0302n", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Aix-Marseille University", 
          "id": "https://www.grid.ac/institutes/grid.5399.6", 
          "name": [
            "Aix Marseille Universit\u00e9, CNRS, INP UMR7051, NeuroCyto, 13015, Marseille, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Caillol", 
        "givenName": "Ghislaine", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Aix-Marseille University", 
          "id": "https://www.grid.ac/institutes/grid.5399.6", 
          "name": [
            "Aix Marseille Universit\u00e9, CNRS, INP UMR7051, NeuroCyto, 13015, Marseille, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Boroni-Rueda", 
        "givenName": "Fanny", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University College London", 
          "id": "https://www.grid.ac/institutes/grid.83440.3b", 
          "name": [
            "MRC-Laboratory for Molecular Cell Biology, University College London, WC1E 6BT, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Dix", 
        "givenName": "Christina L.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University College London", 
          "id": "https://www.grid.ac/institutes/grid.83440.3b", 
          "name": [
            "London Centre for Nanotechnology, WC1H 0AH, London, UK", 
            "Institute for the Physics of Living Systems, University College London, WC1E 6BT, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Charras", 
        "givenName": "Guillaume", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University College London", 
          "id": "https://www.grid.ac/institutes/grid.83440.3b", 
          "name": [
            "MRC-Laboratory for Molecular Cell Biology, University College London, WC1E 6BT, London, UK", 
            "Institute for the Physics of Living Systems, University College London, WC1E 6BT, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Baum", 
        "givenName": "Buzz", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Francis Crick Institute", 
          "id": "https://www.grid.ac/institutes/grid.451388.3", 
          "name": [
            "MRC-Laboratory for Molecular Cell Biology, University College London, WC1E 6BT, London, UK", 
            "The Francis Crick Institute, NW1 1AT, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Laine", 
        "givenName": "Romain F.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Aix-Marseille University", 
          "id": "https://www.grid.ac/institutes/grid.5399.6", 
          "name": [
            "Aix Marseille Universit\u00e9, CNRS, INP UMR7051, NeuroCyto, 13015, Marseille, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Leterrier", 
        "givenName": "Christophe", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Francis Crick Institute", 
          "id": "https://www.grid.ac/institutes/grid.451388.3", 
          "name": [
            "MRC-Laboratory for Molecular Cell Biology, University College London, WC1E 6BT, London, UK", 
            "Department of Cell and Developmental Biology, University College London, WC1E 6BT, London, UK", 
            "The Francis Crick Institute, NW1 1AT, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Henriques", 
        "givenName": "Ricardo", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/j.cell.2008.12.020", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000551999"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncomms12471", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005342322", 
          "https://doi.org/10.1038/ncomms12471"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/bio.019570", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005386746"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.2448", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006928937", 
          "https://doi.org/10.1038/nmeth.2448"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2015.02.033", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007452889"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/cm.20226", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009145438"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0506010102", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012403980"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/jcs.091967", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014174079"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/srep29132", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015355512", 
          "https://doi.org/10.1038/srep29132"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/bs.mcb.2014.10.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016436401"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.3466", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018872159", 
          "https://doi.org/10.1038/nmeth.3466"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth929", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018882864", 
          "https://doi.org/10.1038/nmeth929"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth929", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018882864", 
          "https://doi.org/10.1038/nmeth929"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0600447103", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022558372"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm.2016.75", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024536251", 
          "https://doi.org/10.1038/nrm.2016.75"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.2019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026706357", 
          "https://doi.org/10.1038/nmeth.2019"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/bioinformatics/btu202", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033447186"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.1324", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035549823", 
          "https://doi.org/10.1038/nmeth.1324"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.1324", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035549823", 
          "https://doi.org/10.1038/nmeth.1324"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.2089", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035817137", 
          "https://doi.org/10.1038/nmeth.2089"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/srep07924", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036927576", 
          "https://doi.org/10.1038/srep07924"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.celrep.2015.11.051", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037252553"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/nl103427w", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037668364"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/nl103427w", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037668364"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0101772", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042214749"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.1768", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043491552", 
          "https://doi.org/10.1038/nmeth.1768"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0123941", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047056179"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0123941", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047056179"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.2835", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051021562", 
          "https://doi.org/10.1038/nmeth.2835"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/acs.chemrev.6b00604", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055085382"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1091/mbc.e16-05-0330", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059352128"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c6sc05420j", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074247091"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nprot.2017.024", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085434308", 
          "https://doi.org/10.1038/nprot.2017.024"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.4486", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1092440089", 
          "https://doi.org/10.1038/nmeth.4486"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.4486", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1092440089", 
          "https://doi.org/10.1038/nmeth.4486"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.4605", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1101103348", 
          "https://doi.org/10.1038/nmeth.4605"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.4605", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1101103348", 
          "https://doi.org/10.1038/nmeth.4605"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.devcel.2018.03.009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1103186646"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.devcel.2018.03.009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1103186646"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.aar7042", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1105982052"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/432674", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1107364124"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/432674", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1107364124"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/432674", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1107364124"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1088/1361-6463/ab0261", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1111786300"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1088/1361-6463/ab0261", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1111786300"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1088/1361-6463/ab0261", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1111786300"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2019-12", 
    "datePublishedReg": "2019-12-01", 
    "description": "Combining and multiplexing microscopy approaches is crucial to understand cellular events, but requires elaborate workflows. Here, we present a robust, open-source approach for treating, labelling and imaging live or fixed cells in automated sequences. NanoJ-Fluidics is based on low-cost Lego hardware controlled by ImageJ-based software, making high-content, multimodal imaging easy to implement on any microscope with high reproducibility. We demonstrate its capacity on event-driven, super-resolved live-to-fixed and multiplexed STORM/DNA-PAINT experiments.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/s41467-019-09231-9", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.6620221", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.5138100", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.4106868", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.7746874", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.6944681", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2785939", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.6666258", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1043282", 
        "issn": [
          "2041-1723"
        ], 
        "name": "Nature Communications", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "10"
      }
    ], 
    "name": "Automating multimodal microscopy with NanoJ-Fluidics", 
    "pagination": "1223", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "29772af8f68b12b1bb56b558e1b0b67490ba365039ce078bbd746c0afbcc8d1f"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30874553"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101528555"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/s41467-019-09231-9"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1112759994"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/s41467-019-09231-9", 
      "https://app.dimensions.ai/details/publication/pub.1112759994"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T13:17", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000368_0000000368/records_78935_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/s41467-019-09231-9"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41467-019-09231-9'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41467-019-09231-9'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41467-019-09231-9'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41467-019-09231-9'


 

This table displays all metadata directly associated to this object as RDF triples.

272 TRIPLES      21 PREDICATES      64 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/s41467-019-09231-9 schema:about anzsrc-for:08
2 anzsrc-for:0801
3 schema:author Nfbd1407202d94f489b67d2ee54aef6d3
4 schema:citation sg:pub.10.1038/ncomms12471
5 sg:pub.10.1038/nmeth.1324
6 sg:pub.10.1038/nmeth.1768
7 sg:pub.10.1038/nmeth.2019
8 sg:pub.10.1038/nmeth.2089
9 sg:pub.10.1038/nmeth.2448
10 sg:pub.10.1038/nmeth.2835
11 sg:pub.10.1038/nmeth.3466
12 sg:pub.10.1038/nmeth.4486
13 sg:pub.10.1038/nmeth.4605
14 sg:pub.10.1038/nmeth929
15 sg:pub.10.1038/nprot.2017.024
16 sg:pub.10.1038/nrm.2016.75
17 sg:pub.10.1038/srep07924
18 sg:pub.10.1038/srep29132
19 https://doi.org/10.1002/cm.20226
20 https://doi.org/10.1016/bs.mcb.2014.10.004
21 https://doi.org/10.1016/j.cell.2008.12.020
22 https://doi.org/10.1016/j.celrep.2015.11.051
23 https://doi.org/10.1016/j.devcel.2018.03.009
24 https://doi.org/10.1016/j.molcel.2015.02.033
25 https://doi.org/10.1021/acs.chemrev.6b00604
26 https://doi.org/10.1021/nl103427w
27 https://doi.org/10.1039/c6sc05420j
28 https://doi.org/10.1073/pnas.0506010102
29 https://doi.org/10.1073/pnas.0600447103
30 https://doi.org/10.1088/1361-6463/ab0261
31 https://doi.org/10.1091/mbc.e16-05-0330
32 https://doi.org/10.1093/bioinformatics/btu202
33 https://doi.org/10.1101/432674
34 https://doi.org/10.1126/science.aar7042
35 https://doi.org/10.1242/bio.019570
36 https://doi.org/10.1242/jcs.091967
37 https://doi.org/10.1371/journal.pone.0101772
38 https://doi.org/10.1371/journal.pone.0123941
39 schema:datePublished 2019-12
40 schema:datePublishedReg 2019-12-01
41 schema:description Combining and multiplexing microscopy approaches is crucial to understand cellular events, but requires elaborate workflows. Here, we present a robust, open-source approach for treating, labelling and imaging live or fixed cells in automated sequences. NanoJ-Fluidics is based on low-cost Lego hardware controlled by ImageJ-based software, making high-content, multimodal imaging easy to implement on any microscope with high reproducibility. We demonstrate its capacity on event-driven, super-resolved live-to-fixed and multiplexed STORM/DNA-PAINT experiments.
42 schema:genre research_article
43 schema:inLanguage en
44 schema:isAccessibleForFree true
45 schema:isPartOf N0f1037bdf9434417b7348ae4db1a59ca
46 N3b89a0fd06d244b897699acedc1f5981
47 sg:journal.1043282
48 schema:name Automating multimodal microscopy with NanoJ-Fluidics
49 schema:pagination 1223
50 schema:productId N19ec72c7d5484e6ab301e531789b39e0
51 N4a27c42bc13b49e4bee120f5381a7921
52 N5362a5045d73442fb2bc4131677f1447
53 Na717b350183f417e93414222ef7493b0
54 Ne74d28d645cf49bc9886888cf5c814c4
55 schema:sameAs https://app.dimensions.ai/details/publication/pub.1112759994
56 https://doi.org/10.1038/s41467-019-09231-9
57 schema:sdDatePublished 2019-04-11T13:17
58 schema:sdLicense https://scigraph.springernature.com/explorer/license/
59 schema:sdPublisher N357c65800dc0406d96c400b942e0f527
60 schema:url https://www.nature.com/articles/s41467-019-09231-9
61 sgo:license sg:explorer/license/
62 sgo:sdDataset articles
63 rdf:type schema:ScholarlyArticle
64 N0f1037bdf9434417b7348ae4db1a59ca schema:issueNumber 1
65 rdf:type schema:PublicationIssue
66 N1925eeb657b24f4eb28274302ee5f6aa schema:affiliation https://www.grid.ac/institutes/grid.5399.6
67 schema:familyName Leterrier
68 schema:givenName Christophe
69 rdf:type schema:Person
70 N19ec72c7d5484e6ab301e531789b39e0 schema:name pubmed_id
71 schema:value 30874553
72 rdf:type schema:PropertyValue
73 N1f27fc82025a43ffbfce6e2af97eaeb2 rdf:first N2982f9c67ac840a69c9d4042a92ab73a
74 rdf:rest Nbd14053ec2d9433db967d636712adc32
75 N233b1d0fe2874147b4207174ee581999 rdf:first Nf3b07eb6f266405e887d1f5ee2ce3867
76 rdf:rest N2d698d8d771f4bd5a978655d3c39b8b4
77 N2982f9c67ac840a69c9d4042a92ab73a schema:affiliation https://www.grid.ac/institutes/grid.83440.3b
78 schema:familyName Charras
79 schema:givenName Guillaume
80 rdf:type schema:Person
81 N2d698d8d771f4bd5a978655d3c39b8b4 rdf:first N89333e06f178498886de93099d8cdf5a
82 rdf:rest N1f27fc82025a43ffbfce6e2af97eaeb2
83 N306ce1062fa44c65b193eb01b2ac4ef4 schema:affiliation https://www.grid.ac/institutes/grid.451388.3
84 schema:familyName Henriques
85 schema:givenName Ricardo
86 rdf:type schema:Person
87 N357c65800dc0406d96c400b942e0f527 schema:name Springer Nature - SN SciGraph project
88 rdf:type schema:Organization
89 N3b89a0fd06d244b897699acedc1f5981 schema:volumeNumber 10
90 rdf:type schema:PublicationVolume
91 N3fd6af6a37074b45a7f94bc3528b3f82 rdf:first N45b981fb47c34ad2bcac9c8883a49d49
92 rdf:rest N233b1d0fe2874147b4207174ee581999
93 N45b981fb47c34ad2bcac9c8883a49d49 schema:affiliation https://www.grid.ac/institutes/grid.5399.6
94 schema:familyName Caillol
95 schema:givenName Ghislaine
96 rdf:type schema:Person
97 N49787d02f3414d659b42552e4d148a60 rdf:first Ndc80baf6cacb44ebbf9841b61005df5b
98 rdf:rest N3fd6af6a37074b45a7f94bc3528b3f82
99 N4a27c42bc13b49e4bee120f5381a7921 schema:name readcube_id
100 schema:value 29772af8f68b12b1bb56b558e1b0b67490ba365039ce078bbd746c0afbcc8d1f
101 rdf:type schema:PropertyValue
102 N5362a5045d73442fb2bc4131677f1447 schema:name doi
103 schema:value 10.1038/s41467-019-09231-9
104 rdf:type schema:PropertyValue
105 N629f90ab1e744894a1be9cc24acf2424 rdf:first N1925eeb657b24f4eb28274302ee5f6aa
106 rdf:rest Naef0a57952d34029806af3b4c9d877fa
107 N711216e215984272b6168e77d13befcb schema:affiliation https://www.grid.ac/institutes/grid.451388.3
108 schema:familyName Laine
109 schema:givenName Romain F.
110 rdf:type schema:Person
111 N7192d03256c644ac935e908b91524e7b rdf:first N711216e215984272b6168e77d13befcb
112 rdf:rest N629f90ab1e744894a1be9cc24acf2424
113 N88b92beb0fb9481fb1c89610860c14f7 schema:affiliation https://www.grid.ac/institutes/grid.83440.3b
114 schema:familyName Baum
115 schema:givenName Buzz
116 rdf:type schema:Person
117 N89333e06f178498886de93099d8cdf5a schema:affiliation https://www.grid.ac/institutes/grid.83440.3b
118 schema:familyName Dix
119 schema:givenName Christina L.
120 rdf:type schema:Person
121 N8d1d4af7b35a49dea0e132d0049b7dd3 schema:affiliation https://www.grid.ac/institutes/grid.451388.3
122 schema:familyName Pereira
123 schema:givenName Pedro M.
124 rdf:type schema:Person
125 Na717b350183f417e93414222ef7493b0 schema:name dimensions_id
126 schema:value pub.1112759994
127 rdf:type schema:PropertyValue
128 Naef0a57952d34029806af3b4c9d877fa rdf:first N306ce1062fa44c65b193eb01b2ac4ef4
129 rdf:rest rdf:nil
130 Nbd14053ec2d9433db967d636712adc32 rdf:first N88b92beb0fb9481fb1c89610860c14f7
131 rdf:rest N7192d03256c644ac935e908b91524e7b
132 Ndc80baf6cacb44ebbf9841b61005df5b schema:affiliation https://www.grid.ac/institutes/grid.451388.3
133 schema:familyName Culley
134 schema:givenName Siân
135 rdf:type schema:Person
136 Ne74d28d645cf49bc9886888cf5c814c4 schema:name nlm_unique_id
137 schema:value 101528555
138 rdf:type schema:PropertyValue
139 Ne8cfb8da767848b3b2b19b69a9dab1d7 schema:affiliation https://www.grid.ac/institutes/grid.83440.3b
140 schema:familyName Almada
141 schema:givenName Pedro
142 rdf:type schema:Person
143 Nf3b07eb6f266405e887d1f5ee2ce3867 schema:affiliation https://www.grid.ac/institutes/grid.5399.6
144 schema:familyName Boroni-Rueda
145 schema:givenName Fanny
146 rdf:type schema:Person
147 Nfbd1407202d94f489b67d2ee54aef6d3 rdf:first Ne8cfb8da767848b3b2b19b69a9dab1d7
148 rdf:rest Nfc95904790c04273a39737b9250063c0
149 Nfc95904790c04273a39737b9250063c0 rdf:first N8d1d4af7b35a49dea0e132d0049b7dd3
150 rdf:rest N49787d02f3414d659b42552e4d148a60
151 anzsrc-for:08 schema:inDefinedTermSet anzsrc-for:
152 schema:name Information and Computing Sciences
153 rdf:type schema:DefinedTerm
154 anzsrc-for:0801 schema:inDefinedTermSet anzsrc-for:
155 schema:name Artificial Intelligence and Image Processing
156 rdf:type schema:DefinedTerm
157 sg:grant.2785939 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-019-09231-9
158 rdf:type schema:MonetaryGrant
159 sg:grant.4106868 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-019-09231-9
160 rdf:type schema:MonetaryGrant
161 sg:grant.5138100 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-019-09231-9
162 rdf:type schema:MonetaryGrant
163 sg:grant.6620221 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-019-09231-9
164 rdf:type schema:MonetaryGrant
165 sg:grant.6666258 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-019-09231-9
166 rdf:type schema:MonetaryGrant
167 sg:grant.6944681 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-019-09231-9
168 rdf:type schema:MonetaryGrant
169 sg:grant.7746874 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-019-09231-9
170 rdf:type schema:MonetaryGrant
171 sg:journal.1043282 schema:issn 2041-1723
172 schema:name Nature Communications
173 rdf:type schema:Periodical
174 sg:pub.10.1038/ncomms12471 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005342322
175 https://doi.org/10.1038/ncomms12471
176 rdf:type schema:CreativeWork
177 sg:pub.10.1038/nmeth.1324 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035549823
178 https://doi.org/10.1038/nmeth.1324
179 rdf:type schema:CreativeWork
180 sg:pub.10.1038/nmeth.1768 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043491552
181 https://doi.org/10.1038/nmeth.1768
182 rdf:type schema:CreativeWork
183 sg:pub.10.1038/nmeth.2019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026706357
184 https://doi.org/10.1038/nmeth.2019
185 rdf:type schema:CreativeWork
186 sg:pub.10.1038/nmeth.2089 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035817137
187 https://doi.org/10.1038/nmeth.2089
188 rdf:type schema:CreativeWork
189 sg:pub.10.1038/nmeth.2448 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006928937
190 https://doi.org/10.1038/nmeth.2448
191 rdf:type schema:CreativeWork
192 sg:pub.10.1038/nmeth.2835 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051021562
193 https://doi.org/10.1038/nmeth.2835
194 rdf:type schema:CreativeWork
195 sg:pub.10.1038/nmeth.3466 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018872159
196 https://doi.org/10.1038/nmeth.3466
197 rdf:type schema:CreativeWork
198 sg:pub.10.1038/nmeth.4486 schema:sameAs https://app.dimensions.ai/details/publication/pub.1092440089
199 https://doi.org/10.1038/nmeth.4486
200 rdf:type schema:CreativeWork
201 sg:pub.10.1038/nmeth.4605 schema:sameAs https://app.dimensions.ai/details/publication/pub.1101103348
202 https://doi.org/10.1038/nmeth.4605
203 rdf:type schema:CreativeWork
204 sg:pub.10.1038/nmeth929 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018882864
205 https://doi.org/10.1038/nmeth929
206 rdf:type schema:CreativeWork
207 sg:pub.10.1038/nprot.2017.024 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085434308
208 https://doi.org/10.1038/nprot.2017.024
209 rdf:type schema:CreativeWork
210 sg:pub.10.1038/nrm.2016.75 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024536251
211 https://doi.org/10.1038/nrm.2016.75
212 rdf:type schema:CreativeWork
213 sg:pub.10.1038/srep07924 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036927576
214 https://doi.org/10.1038/srep07924
215 rdf:type schema:CreativeWork
216 sg:pub.10.1038/srep29132 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015355512
217 https://doi.org/10.1038/srep29132
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1002/cm.20226 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009145438
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1016/bs.mcb.2014.10.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016436401
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1016/j.cell.2008.12.020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000551999
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1016/j.celrep.2015.11.051 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037252553
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1016/j.devcel.2018.03.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1103186646
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1016/j.molcel.2015.02.033 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007452889
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1021/acs.chemrev.6b00604 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055085382
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1021/nl103427w schema:sameAs https://app.dimensions.ai/details/publication/pub.1037668364
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1039/c6sc05420j schema:sameAs https://app.dimensions.ai/details/publication/pub.1074247091
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1073/pnas.0506010102 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012403980
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1073/pnas.0600447103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022558372
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1088/1361-6463/ab0261 schema:sameAs https://app.dimensions.ai/details/publication/pub.1111786300
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1091/mbc.e16-05-0330 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059352128
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1093/bioinformatics/btu202 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033447186
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1101/432674 schema:sameAs https://app.dimensions.ai/details/publication/pub.1107364124
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1126/science.aar7042 schema:sameAs https://app.dimensions.ai/details/publication/pub.1105982052
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1242/bio.019570 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005386746
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1242/jcs.091967 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014174079
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1371/journal.pone.0101772 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042214749
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1371/journal.pone.0123941 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047056179
258 rdf:type schema:CreativeWork
259 https://www.grid.ac/institutes/grid.451388.3 schema:alternateName The Francis Crick Institute
260 schema:name Department of Cell and Developmental Biology, University College London, WC1E 6BT, London, UK
261 MRC-Laboratory for Molecular Cell Biology, University College London, WC1E 6BT, London, UK
262 The Francis Crick Institute, NW1 1AT, London, UK
263 rdf:type schema:Organization
264 https://www.grid.ac/institutes/grid.5399.6 schema:alternateName Aix-Marseille University
265 schema:name Aix Marseille Université, CNRS, INP UMR7051, NeuroCyto, 13015, Marseille, France
266 rdf:type schema:Organization
267 https://www.grid.ac/institutes/grid.83440.3b schema:alternateName University College London
268 schema:name Department of Cell and Developmental Biology, University College London, WC1E 6BT, London, UK
269 Institute for the Physics of Living Systems, University College London, WC1E 6BT, London, UK
270 London Centre for Nanotechnology, WC1H 0AH, London, UK
271 MRC-Laboratory for Molecular Cell Biology, University College London, WC1E 6BT, London, UK
272 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...