Molecular architecture of a cylindrical self-assembly at human centrosomes View Full Text


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Article Info

DATE

2019-03-11

AUTHORS

Tae-Sung Kim, Liang Zhang, Jong Il Ahn, Lingjun Meng, Yang Chen, Eunhye Lee, Jeong Kyu Bang, Jung Mi Lim, Rodolfo Ghirlando, Lixin Fan, Yun-Xing Wang, Bo Yeon Kim, Jung-Eun Park, Kyung S. Lee

ABSTRACT

The cell is constructed by higher-order structures and organelles through complex interactions among distinct structural constituents. The centrosome is a membraneless organelle composed of two microtubule-derived structures called centrioles and an amorphous mass of pericentriolar material. Super-resolution microscopic analyses in various organisms revealed that diverse pericentriolar material proteins are concentrically localized around a centriole in a highly organized manner. However, the molecular nature underlying these organizations remains unknown. Here we show that two human pericentriolar material scaffolds, Cep63 and Cep152, cooperatively generate a heterotetrameric α-helical bundle that functions in conjunction with its neighboring hydrophobic motifs to self-assemble into a higher-order cylindrical architecture capable of recruiting downstream components, including Plk4, a key regulator for centriole duplication. Mutations disrupting the self-assembly abrogate Plk4-mediated centriole duplication. Because pericentriolar material organization is evolutionarily conserved, this work may offer a paradigm for investigating the assembly and function of centrosomal scaffolds in various organisms. More... »

PAGES

1151

References to SciGraph publications

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41467-019-08838-2

    DOI

    http://dx.doi.org/10.1038/s41467-019-08838-2

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1112674246

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30858376


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