High-throughput single-cell rheology in complex samples by dynamic real-time deformability cytometry View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Bob Fregin, Fabian Czerwinski, Doreen Biedenweg, Salvatore Girardo, Stefan Gross, Konstanze Aurich, Oliver Otto

ABSTRACT

In life sciences, the material properties of suspended cells have attained significance close to that of fluorescent markers but with the advantage of label-free and unbiased sample characterization. Until recently, cell rheological measurements were either limited by acquisition throughput, excessive post processing, or low-throughput real-time analysis. Real-time deformability cytometry expanded the application of mechanical cell assays to fast on-the-fly phenotyping of large sample sizes, but has been restricted to single material parameters as the Young's modulus. Here, we introduce dynamic real-time deformability cytometry for comprehensive cell rheological measurements at up to 100 cells per second. Utilizing Fourier decomposition, our microfluidic method is able to disentangle cell response to complex hydrodynamic stress distributions and to determine viscoelastic parameters independent of cell shape. We demonstrate the application of our technology for peripheral blood cells in whole blood samples including the discrimination of B- and CD4+ T-lymphocytes by cell rheological properties. More... »

PAGES

415

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41467-019-08370-3

DOI

http://dx.doi.org/10.1038/s41467-019-08370-3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1111649246

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30679420


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Blood Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Shape", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cytochalasin D", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Elasticity", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Flow Cytometry", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "HL-60 Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hydrodynamics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microfluidics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Models, Biological", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phenotype", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rheology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Single-Cell Analysis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Viscosity", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Greifswald", 
          "id": "https://www.grid.ac/institutes/grid.5603.0", 
          "name": [
            "Zentrum f\u00fcr Innovationskompetenz: Humorale Immunreaktionen bei kardiovaskul\u00e4ren Erkrankungen, Universit\u00e4t Greifswald, Fleischmannstr. 42, 17489, Greifswald, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fregin", 
        "givenName": "Bob", 
        "id": "sg:person.011630317015.17", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011630317015.17"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Greifswald", 
          "id": "https://www.grid.ac/institutes/grid.5603.0", 
          "name": [
            "Zentrum f\u00fcr Innovationskompetenz: Humorale Immunreaktionen bei kardiovaskul\u00e4ren Erkrankungen, Universit\u00e4t Greifswald, Fleischmannstr. 42, 17489, Greifswald, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Czerwinski", 
        "givenName": "Fabian", 
        "id": "sg:person.01136437366.53", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01136437366.53"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Greifswald", 
          "id": "https://www.grid.ac/institutes/grid.5603.0", 
          "name": [
            "Universit\u00e4tsmedizin Greifswald, Fleischmannstr. 8, 17489, Greifswald, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Biedenweg", 
        "givenName": "Doreen", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "TU Dresden", 
          "id": "https://www.grid.ac/institutes/grid.4488.0", 
          "name": [
            "Biotechnology Center, Center for Molecular and Cellular Bioengineering, Technische Universit\u00e4t Dresden, Tatzberg 47/49, 01307, Dresden, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Girardo", 
        "givenName": "Salvatore", 
        "id": "sg:person.01316772346.49", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01316772346.49"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Greifswald", 
          "id": "https://www.grid.ac/institutes/grid.5603.0", 
          "name": [
            "Universit\u00e4tsmedizin Greifswald, Fleischmannstr. 8, 17489, Greifswald, Germany", 
            "Deutsches Zentrum f\u00fcr Herz-Kreislauf-Forschung e.V., Standort Greifswald, Universit\u00e4tsmedizin Greifswald, Fleischmannstr. 42, 17489, Greifswald, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gross", 
        "givenName": "Stefan", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Greifswald", 
          "id": "https://www.grid.ac/institutes/grid.5603.0", 
          "name": [
            "Universit\u00e4tsmedizin Greifswald, Fleischmannstr. 8, 17489, Greifswald, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Aurich", 
        "givenName": "Konstanze", 
        "id": "sg:person.01046476275.38", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01046476275.38"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Greifswald", 
          "id": "https://www.grid.ac/institutes/grid.5603.0", 
          "name": [
            "Zentrum f\u00fcr Innovationskompetenz: Humorale Immunreaktionen bei kardiovaskul\u00e4ren Erkrankungen, Universit\u00e4t Greifswald, Fleischmannstr. 42, 17489, Greifswald, Germany", 
            "Deutsches Zentrum f\u00fcr Herz-Kreislauf-Forschung e.V., Standort Greifswald, Universit\u00e4tsmedizin Greifswald, Fleischmannstr. 42, 17489, Greifswald, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Otto", 
        "givenName": "Oliver", 
        "id": "sg:person.0611343320.08", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0611343320.08"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.7554/elife.09347", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002183604"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.chom.2012.10.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009732790"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nphys3619", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011009011", 
          "https://doi.org/10.1038/nphys3619"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0045237", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012124215"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature05824", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013628212", 
          "https://doi.org/10.1038/nature05824"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/b97636", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016630193", 
          "https://doi.org/10.1007/b97636"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/b97636", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016630193", 
          "https://doi.org/10.1007/b97636"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/b97636", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016630193", 
          "https://doi.org/10.1007/b97636"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c4lc01251h", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017110732"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature08908", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019067497", 
          "https://doi.org/10.1038/nature08908"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature08908", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019067497", 
          "https://doi.org/10.1038/nature08908"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-1759(95)00078-o", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019611006"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c5ib00304k", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021414527"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bpj.2015.09.006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022398682"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1088/1742-6596/530/1/012012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024421914"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bpj.2010.08.052", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024873295"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1529/biophysj.104.050278", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025148306"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev-matsci-062910-100351", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025952799"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.3281", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026034813", 
          "https://doi.org/10.1038/nmeth.3281"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0811261106", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026592466"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0811261106", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026592466"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0006-3495(01)75740-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027419753"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmat1685", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028503805", 
          "https://doi.org/10.1038/nmat1685"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmat1685", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028503805", 
          "https://doi.org/10.1038/nmat1685"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0805779105", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029362177"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c5ib00284b", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029418287"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bpj.2016.09.034", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033030994"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bpj.2016.09.034", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033030994"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3390/polym7050777", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035617945"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1103/physrevlett.108.178103", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038962948"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1103/physrevlett.108.178103", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038962948"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bpj.2015.05.029", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040803509"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/srep27238", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044295360", 
          "https://doi.org/10.1038/srep27238"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1200107109", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047090690"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00216-010-3721-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047257581", 
          "https://doi.org/10.1007/s00216-010-3721-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00216-010-3721-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047257581", 
          "https://doi.org/10.1007/s00216-010-3721-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0006-3495(01)75953-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048700285"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmat3943", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049335424", 
          "https://doi.org/10.1038/nmat3943"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ac402920m", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055004966"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/acsbiomaterials.6b00558", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055133278"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1103/physrevlett.84.5451", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060821438"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1103/physrevlett.84.5451", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060821438"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1103/physrevlett.87.148102", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060823785"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1103/physrevlett.87.148102", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060823785"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.2749255", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062555344"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/scitranslmed.3007731", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062688189"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1620843114", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084510625"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bpj.2017.02.018", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084772166"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nphys4104", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085099295", 
          "https://doi.org/10.1038/nphys4104"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nphys4104", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085099295", 
          "https://doi.org/10.1038/nphys4104"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/cm.21369", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085204026"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/s41598-017-06311-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1090859668", 
          "https://doi.org/10.1038/s41598-017-06311-y"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bpj.2017.06.073", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1092049570"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/cshperspect.a022038", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1092479914"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.7554/elife.29213", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1100393886"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/acs.analchem.7b05143", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1100669784"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.4639", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1101887190", 
          "https://doi.org/10.1038/nmeth.4639"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.4639", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1101887190", 
          "https://doi.org/10.1038/nmeth.4639"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.4639", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1101887190", 
          "https://doi.org/10.1038/nmeth.4639"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1063/1.5027197", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1104381814"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c8tb01421c", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1106575823"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2019-12", 
    "datePublishedReg": "2019-12-01", 
    "description": "In life sciences, the material properties of suspended cells have attained significance close to that of fluorescent markers but with the advantage of label-free and unbiased sample characterization. Until recently, cell rheological measurements were either limited by acquisition throughput, excessive post processing, or low-throughput real-time analysis. Real-time deformability cytometry expanded the application of mechanical cell assays to fast on-the-fly phenotyping of large sample sizes, but has been restricted to single material parameters as the Young's modulus. Here, we introduce dynamic real-time deformability cytometry for comprehensive cell rheological measurements at up to 100 cells per second. Utilizing Fourier decomposition, our microfluidic method is able to disentangle cell response to complex hydrodynamic stress distributions and to determine viscoelastic parameters independent of cell shape. We demonstrate the application of our technology for peripheral blood cells in whole blood samples including the discrimination of B- and CD4+ T-lymphocytes by cell rheological properties.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/s41467-019-08370-3", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.7706944", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1043282", 
        "issn": [
          "2041-1723"
        ], 
        "name": "Nature Communications", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "10"
      }
    ], 
    "name": "High-throughput single-cell rheology in complex samples by dynamic real-time deformability cytometry", 
    "pagination": "415", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "befdfc0c175a6f90f860d63dab0c78882a77d5c337a3b9add03fde5e98decdcf"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30679420"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101528555"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/s41467-019-08370-3"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1111649246"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/s41467-019-08370-3", 
      "https://app.dimensions.ai/details/publication/pub.1111649246"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T09:41", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000346_0000000346/records_99841_00000004.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/s41467-019-08370-3"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41467-019-08370-3'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41467-019-08370-3'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41467-019-08370-3'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41467-019-08370-3'


 

This table displays all metadata directly associated to this object as RDF triples.

327 TRIPLES      21 PREDICATES      91 URIs      35 LITERALS      23 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/s41467-019-08370-3 schema:about N03eaa5cd5bb64e498b0bed57246a81b5
2 N05069fca71d24ffea337bfffb3fe34f8
3 N311070c6c1d347de9d15c2414d329653
4 N4f85ea8a293347e0b41e7f91297a9f17
5 N5753f59718df471282777c2d3104c1cb
6 N6fa1467398034f42b18b41710b165923
7 N71a602ecc8b348fdb035c9e56df95cb3
8 N749cb86db4664f0180fb97d23913044e
9 N980b34dc93b34042801fedd1d6b845b2
10 Na2d9ab146dac43f588041da8d5fbc6cb
11 Nbe1a7a280ae442ac9a960f52affd1842
12 Nd771594c51a44890a067ff710613a83e
13 Ne6c4284bb93f415795c2ea4777054088
14 Nf72393dc392b433493ccac8cb2273f3d
15 anzsrc-for:06
16 anzsrc-for:0601
17 schema:author N92f4818ac7e543bba183599b635bb668
18 schema:citation sg:pub.10.1007/b97636
19 sg:pub.10.1007/s00216-010-3721-9
20 sg:pub.10.1038/nature05824
21 sg:pub.10.1038/nature08908
22 sg:pub.10.1038/nmat1685
23 sg:pub.10.1038/nmat3943
24 sg:pub.10.1038/nmeth.3281
25 sg:pub.10.1038/nmeth.4639
26 sg:pub.10.1038/nphys3619
27 sg:pub.10.1038/nphys4104
28 sg:pub.10.1038/s41598-017-06311-y
29 sg:pub.10.1038/srep27238
30 https://doi.org/10.1002/cm.21369
31 https://doi.org/10.1016/0022-1759(95)00078-o
32 https://doi.org/10.1016/j.bpj.2010.08.052
33 https://doi.org/10.1016/j.bpj.2015.05.029
34 https://doi.org/10.1016/j.bpj.2015.09.006
35 https://doi.org/10.1016/j.bpj.2016.09.034
36 https://doi.org/10.1016/j.bpj.2017.02.018
37 https://doi.org/10.1016/j.bpj.2017.06.073
38 https://doi.org/10.1016/j.chom.2012.10.001
39 https://doi.org/10.1016/s0006-3495(01)75740-2
40 https://doi.org/10.1016/s0006-3495(01)75953-x
41 https://doi.org/10.1021/ac402920m
42 https://doi.org/10.1021/acs.analchem.7b05143
43 https://doi.org/10.1021/acsbiomaterials.6b00558
44 https://doi.org/10.1039/c4lc01251h
45 https://doi.org/10.1039/c5ib00284b
46 https://doi.org/10.1039/c5ib00304k
47 https://doi.org/10.1039/c8tb01421c
48 https://doi.org/10.1063/1.5027197
49 https://doi.org/10.1073/pnas.0805779105
50 https://doi.org/10.1073/pnas.0811261106
51 https://doi.org/10.1073/pnas.1200107109
52 https://doi.org/10.1073/pnas.1620843114
53 https://doi.org/10.1088/1742-6596/530/1/012012
54 https://doi.org/10.1101/cshperspect.a022038
55 https://doi.org/10.1103/physrevlett.108.178103
56 https://doi.org/10.1103/physrevlett.84.5451
57 https://doi.org/10.1103/physrevlett.87.148102
58 https://doi.org/10.1126/science.2749255
59 https://doi.org/10.1126/scitranslmed.3007731
60 https://doi.org/10.1146/annurev-matsci-062910-100351
61 https://doi.org/10.1371/journal.pone.0045237
62 https://doi.org/10.1529/biophysj.104.050278
63 https://doi.org/10.3390/polym7050777
64 https://doi.org/10.7554/elife.09347
65 https://doi.org/10.7554/elife.29213
66 schema:datePublished 2019-12
67 schema:datePublishedReg 2019-12-01
68 schema:description In life sciences, the material properties of suspended cells have attained significance close to that of fluorescent markers but with the advantage of label-free and unbiased sample characterization. Until recently, cell rheological measurements were either limited by acquisition throughput, excessive post processing, or low-throughput real-time analysis. Real-time deformability cytometry expanded the application of mechanical cell assays to fast on-the-fly phenotyping of large sample sizes, but has been restricted to single material parameters as the Young's modulus. Here, we introduce dynamic real-time deformability cytometry for comprehensive cell rheological measurements at up to 100 cells per second. Utilizing Fourier decomposition, our microfluidic method is able to disentangle cell response to complex hydrodynamic stress distributions and to determine viscoelastic parameters independent of cell shape. We demonstrate the application of our technology for peripheral blood cells in whole blood samples including the discrimination of B- and CD4+ T-lymphocytes by cell rheological properties.
69 schema:genre research_article
70 schema:inLanguage en
71 schema:isAccessibleForFree true
72 schema:isPartOf N2cbcc37994af46b297b03bcb14311ff0
73 Ne0e09078e6a649318353af0e9f667823
74 sg:journal.1043282
75 schema:name High-throughput single-cell rheology in complex samples by dynamic real-time deformability cytometry
76 schema:pagination 415
77 schema:productId N307b87bf9f98422abe8b8237e8d499f4
78 N6001833d22b1472297a1f94df93c3bf9
79 N6faf41d5cbc143a79f4d3f47f6c4c448
80 N8af1a00afa484bc7baad83302bfa1c8c
81 Ndd93f228596d4758a5e140421585bec9
82 schema:sameAs https://app.dimensions.ai/details/publication/pub.1111649246
83 https://doi.org/10.1038/s41467-019-08370-3
84 schema:sdDatePublished 2019-04-11T09:41
85 schema:sdLicense https://scigraph.springernature.com/explorer/license/
86 schema:sdPublisher N47cb4f4ddb8e4be692dc93a298278f27
87 schema:url https://www.nature.com/articles/s41467-019-08370-3
88 sgo:license sg:explorer/license/
89 sgo:sdDataset articles
90 rdf:type schema:ScholarlyArticle
91 N03eaa5cd5bb64e498b0bed57246a81b5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
92 schema:name Phenotype
93 rdf:type schema:DefinedTerm
94 N05069fca71d24ffea337bfffb3fe34f8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
95 schema:name Hydrodynamics
96 rdf:type schema:DefinedTerm
97 N11ffe6d2a66046029ef623b62dafe5e1 rdf:first sg:person.0611343320.08
98 rdf:rest rdf:nil
99 N1a77cd903ac54ec4bbece547c09257d2 schema:affiliation https://www.grid.ac/institutes/grid.5603.0
100 schema:familyName Biedenweg
101 schema:givenName Doreen
102 rdf:type schema:Person
103 N2cbcc37994af46b297b03bcb14311ff0 schema:issueNumber 1
104 rdf:type schema:PublicationIssue
105 N302eafd53ccb469998b2ae50bdc4021e rdf:first sg:person.01136437366.53
106 rdf:rest N62519080542c415fa907226b7f4d6e5c
107 N307b87bf9f98422abe8b8237e8d499f4 schema:name readcube_id
108 schema:value befdfc0c175a6f90f860d63dab0c78882a77d5c337a3b9add03fde5e98decdcf
109 rdf:type schema:PropertyValue
110 N311070c6c1d347de9d15c2414d329653 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Microfluidics
112 rdf:type schema:DefinedTerm
113 N4185d797c4814e259aa4b502a6507776 rdf:first sg:person.01316772346.49
114 rdf:rest Ncf96e2a41f27401c93f1b0bf49301d67
115 N47cb4f4ddb8e4be692dc93a298278f27 schema:name Springer Nature - SN SciGraph project
116 rdf:type schema:Organization
117 N4f85ea8a293347e0b41e7f91297a9f17 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Cytochalasin D
119 rdf:type schema:DefinedTerm
120 N5753f59718df471282777c2d3104c1cb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Cell Shape
122 rdf:type schema:DefinedTerm
123 N6001833d22b1472297a1f94df93c3bf9 schema:name nlm_unique_id
124 schema:value 101528555
125 rdf:type schema:PropertyValue
126 N62519080542c415fa907226b7f4d6e5c rdf:first N1a77cd903ac54ec4bbece547c09257d2
127 rdf:rest N4185d797c4814e259aa4b502a6507776
128 N6fa1467398034f42b18b41710b165923 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name Viscosity
130 rdf:type schema:DefinedTerm
131 N6faf41d5cbc143a79f4d3f47f6c4c448 schema:name pubmed_id
132 schema:value 30679420
133 rdf:type schema:PropertyValue
134 N71a602ecc8b348fdb035c9e56df95cb3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name Models, Biological
136 rdf:type schema:DefinedTerm
137 N749cb86db4664f0180fb97d23913044e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Rheology
139 rdf:type schema:DefinedTerm
140 N8af1a00afa484bc7baad83302bfa1c8c schema:name dimensions_id
141 schema:value pub.1111649246
142 rdf:type schema:PropertyValue
143 N92f4818ac7e543bba183599b635bb668 rdf:first sg:person.011630317015.17
144 rdf:rest N302eafd53ccb469998b2ae50bdc4021e
145 N980b34dc93b34042801fedd1d6b845b2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name HL-60 Cells
147 rdf:type schema:DefinedTerm
148 Na2d9ab146dac43f588041da8d5fbc6cb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
149 schema:name Flow Cytometry
150 rdf:type schema:DefinedTerm
151 Nbe1a7a280ae442ac9a960f52affd1842 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name Single-Cell Analysis
153 rdf:type schema:DefinedTerm
154 Ncf96e2a41f27401c93f1b0bf49301d67 rdf:first Nd4395f80870e44cd98cf1baf3b403d0b
155 rdf:rest Nfc62bc0d653d495dad2d466575e2967a
156 Nd4395f80870e44cd98cf1baf3b403d0b schema:affiliation https://www.grid.ac/institutes/grid.5603.0
157 schema:familyName Gross
158 schema:givenName Stefan
159 rdf:type schema:Person
160 Nd771594c51a44890a067ff710613a83e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
161 schema:name Humans
162 rdf:type schema:DefinedTerm
163 Ndd93f228596d4758a5e140421585bec9 schema:name doi
164 schema:value 10.1038/s41467-019-08370-3
165 rdf:type schema:PropertyValue
166 Ne0e09078e6a649318353af0e9f667823 schema:volumeNumber 10
167 rdf:type schema:PublicationVolume
168 Ne6c4284bb93f415795c2ea4777054088 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
169 schema:name Blood Cells
170 rdf:type schema:DefinedTerm
171 Nf72393dc392b433493ccac8cb2273f3d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
172 schema:name Elasticity
173 rdf:type schema:DefinedTerm
174 Nfc62bc0d653d495dad2d466575e2967a rdf:first sg:person.01046476275.38
175 rdf:rest N11ffe6d2a66046029ef623b62dafe5e1
176 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
177 schema:name Biological Sciences
178 rdf:type schema:DefinedTerm
179 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
180 schema:name Biochemistry and Cell Biology
181 rdf:type schema:DefinedTerm
182 sg:grant.7706944 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-019-08370-3
183 rdf:type schema:MonetaryGrant
184 sg:journal.1043282 schema:issn 2041-1723
185 schema:name Nature Communications
186 rdf:type schema:Periodical
187 sg:person.01046476275.38 schema:affiliation https://www.grid.ac/institutes/grid.5603.0
188 schema:familyName Aurich
189 schema:givenName Konstanze
190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01046476275.38
191 rdf:type schema:Person
192 sg:person.01136437366.53 schema:affiliation https://www.grid.ac/institutes/grid.5603.0
193 schema:familyName Czerwinski
194 schema:givenName Fabian
195 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01136437366.53
196 rdf:type schema:Person
197 sg:person.011630317015.17 schema:affiliation https://www.grid.ac/institutes/grid.5603.0
198 schema:familyName Fregin
199 schema:givenName Bob
200 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011630317015.17
201 rdf:type schema:Person
202 sg:person.01316772346.49 schema:affiliation https://www.grid.ac/institutes/grid.4488.0
203 schema:familyName Girardo
204 schema:givenName Salvatore
205 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01316772346.49
206 rdf:type schema:Person
207 sg:person.0611343320.08 schema:affiliation https://www.grid.ac/institutes/grid.5603.0
208 schema:familyName Otto
209 schema:givenName Oliver
210 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0611343320.08
211 rdf:type schema:Person
212 sg:pub.10.1007/b97636 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016630193
213 https://doi.org/10.1007/b97636
214 rdf:type schema:CreativeWork
215 sg:pub.10.1007/s00216-010-3721-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047257581
216 https://doi.org/10.1007/s00216-010-3721-9
217 rdf:type schema:CreativeWork
218 sg:pub.10.1038/nature05824 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013628212
219 https://doi.org/10.1038/nature05824
220 rdf:type schema:CreativeWork
221 sg:pub.10.1038/nature08908 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019067497
222 https://doi.org/10.1038/nature08908
223 rdf:type schema:CreativeWork
224 sg:pub.10.1038/nmat1685 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028503805
225 https://doi.org/10.1038/nmat1685
226 rdf:type schema:CreativeWork
227 sg:pub.10.1038/nmat3943 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049335424
228 https://doi.org/10.1038/nmat3943
229 rdf:type schema:CreativeWork
230 sg:pub.10.1038/nmeth.3281 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026034813
231 https://doi.org/10.1038/nmeth.3281
232 rdf:type schema:CreativeWork
233 sg:pub.10.1038/nmeth.4639 schema:sameAs https://app.dimensions.ai/details/publication/pub.1101887190
234 https://doi.org/10.1038/nmeth.4639
235 rdf:type schema:CreativeWork
236 sg:pub.10.1038/nphys3619 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011009011
237 https://doi.org/10.1038/nphys3619
238 rdf:type schema:CreativeWork
239 sg:pub.10.1038/nphys4104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085099295
240 https://doi.org/10.1038/nphys4104
241 rdf:type schema:CreativeWork
242 sg:pub.10.1038/s41598-017-06311-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1090859668
243 https://doi.org/10.1038/s41598-017-06311-y
244 rdf:type schema:CreativeWork
245 sg:pub.10.1038/srep27238 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044295360
246 https://doi.org/10.1038/srep27238
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1002/cm.21369 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085204026
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1016/0022-1759(95)00078-o schema:sameAs https://app.dimensions.ai/details/publication/pub.1019611006
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1016/j.bpj.2010.08.052 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024873295
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1016/j.bpj.2015.05.029 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040803509
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1016/j.bpj.2015.09.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022398682
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1016/j.bpj.2016.09.034 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033030994
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1016/j.bpj.2017.02.018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084772166
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1016/j.bpj.2017.06.073 schema:sameAs https://app.dimensions.ai/details/publication/pub.1092049570
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1016/j.chom.2012.10.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009732790
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1016/s0006-3495(01)75740-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027419753
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1016/s0006-3495(01)75953-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1048700285
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1021/ac402920m schema:sameAs https://app.dimensions.ai/details/publication/pub.1055004966
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1021/acs.analchem.7b05143 schema:sameAs https://app.dimensions.ai/details/publication/pub.1100669784
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1021/acsbiomaterials.6b00558 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055133278
275 rdf:type schema:CreativeWork
276 https://doi.org/10.1039/c4lc01251h schema:sameAs https://app.dimensions.ai/details/publication/pub.1017110732
277 rdf:type schema:CreativeWork
278 https://doi.org/10.1039/c5ib00284b schema:sameAs https://app.dimensions.ai/details/publication/pub.1029418287
279 rdf:type schema:CreativeWork
280 https://doi.org/10.1039/c5ib00304k schema:sameAs https://app.dimensions.ai/details/publication/pub.1021414527
281 rdf:type schema:CreativeWork
282 https://doi.org/10.1039/c8tb01421c schema:sameAs https://app.dimensions.ai/details/publication/pub.1106575823
283 rdf:type schema:CreativeWork
284 https://doi.org/10.1063/1.5027197 schema:sameAs https://app.dimensions.ai/details/publication/pub.1104381814
285 rdf:type schema:CreativeWork
286 https://doi.org/10.1073/pnas.0805779105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029362177
287 rdf:type schema:CreativeWork
288 https://doi.org/10.1073/pnas.0811261106 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026592466
289 rdf:type schema:CreativeWork
290 https://doi.org/10.1073/pnas.1200107109 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047090690
291 rdf:type schema:CreativeWork
292 https://doi.org/10.1073/pnas.1620843114 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084510625
293 rdf:type schema:CreativeWork
294 https://doi.org/10.1088/1742-6596/530/1/012012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024421914
295 rdf:type schema:CreativeWork
296 https://doi.org/10.1101/cshperspect.a022038 schema:sameAs https://app.dimensions.ai/details/publication/pub.1092479914
297 rdf:type schema:CreativeWork
298 https://doi.org/10.1103/physrevlett.108.178103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038962948
299 rdf:type schema:CreativeWork
300 https://doi.org/10.1103/physrevlett.84.5451 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060821438
301 rdf:type schema:CreativeWork
302 https://doi.org/10.1103/physrevlett.87.148102 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060823785
303 rdf:type schema:CreativeWork
304 https://doi.org/10.1126/science.2749255 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062555344
305 rdf:type schema:CreativeWork
306 https://doi.org/10.1126/scitranslmed.3007731 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062688189
307 rdf:type schema:CreativeWork
308 https://doi.org/10.1146/annurev-matsci-062910-100351 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025952799
309 rdf:type schema:CreativeWork
310 https://doi.org/10.1371/journal.pone.0045237 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012124215
311 rdf:type schema:CreativeWork
312 https://doi.org/10.1529/biophysj.104.050278 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025148306
313 rdf:type schema:CreativeWork
314 https://doi.org/10.3390/polym7050777 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035617945
315 rdf:type schema:CreativeWork
316 https://doi.org/10.7554/elife.09347 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002183604
317 rdf:type schema:CreativeWork
318 https://doi.org/10.7554/elife.29213 schema:sameAs https://app.dimensions.ai/details/publication/pub.1100393886
319 rdf:type schema:CreativeWork
320 https://www.grid.ac/institutes/grid.4488.0 schema:alternateName TU Dresden
321 schema:name Biotechnology Center, Center for Molecular and Cellular Bioengineering, Technische Universität Dresden, Tatzberg 47/49, 01307, Dresden, Germany
322 rdf:type schema:Organization
323 https://www.grid.ac/institutes/grid.5603.0 schema:alternateName University of Greifswald
324 schema:name Deutsches Zentrum für Herz-Kreislauf-Forschung e.V., Standort Greifswald, Universitätsmedizin Greifswald, Fleischmannstr. 42, 17489, Greifswald, Germany
325 Universitätsmedizin Greifswald, Fleischmannstr. 8, 17489, Greifswald, Germany
326 Zentrum für Innovationskompetenz: Humorale Immunreaktionen bei kardiovaskulären Erkrankungen, Universität Greifswald, Fleischmannstr. 42, 17489, Greifswald, Germany
327 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...