Ontology type: schema:ScholarlyArticle Open Access: True
2018-12-19
AUTHORSYoung-Jun Park, Kaitlyn D. Lacourse, Christian Cambillau, Frank DiMaio, Joseph D. Mougous, David Veesler
ABSTRACTType VI secretion systems (T6SSs) translocate effectors into target cells and are made of a contractile sheath and a tube docked onto a multi-protein transmembrane complex via a baseplate. Although some information is available about the mechanisms of tail contraction leading to effector delivery, the detailed architecture and function of the baseplate remain unknown. Here, we report the 3.7 Å resolution cryo-electron microscopy reconstruction of an enteroaggregative Escherichia coli baseplate subcomplex assembled from TssK, TssF and TssG. The structure reveals two TssK trimers interact with a locally pseudo-3-fold symmetrical complex comprising two copies of TssF and one copy of TssG. TssF and TssG are structurally related to each other and to components of the phage T4 baseplate and of the type IV secretion system, strengthening the evolutionary relationships among these macromolecular machines. These results, together with bacterial two-hybrid assays, provide a structural framework to understand the T6SS baseplate architecture. More... »
PAGES5385
http://scigraph.springernature.com/pub.10.1038/s41467-018-07796-5
DOIhttp://dx.doi.org/10.1038/s41467-018-07796-5
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1110584070
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/30568167
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biochemistry and Cell Biology",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Amino Acid Sequence",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cryoelectron Microscopy",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Escherichia coli",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Escherichia coli Proteins",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Protein Structure, Quaternary",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Type VI Secretion Systems",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Department of Biochemistry, University of Washington, 98195, Seattle, WA, USA",
"id": "http://www.grid.ac/institutes/grid.34477.33",
"name": [
"Department of Biochemistry, University of Washington, 98195, Seattle, WA, USA"
],
"type": "Organization"
},
"familyName": "Park",
"givenName": "Young-Jun",
"id": "sg:person.015020273740.07",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015020273740.07"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Microbiology, University of Washington, 98195, Seattle, WA, USA",
"id": "http://www.grid.ac/institutes/grid.34477.33",
"name": [
"Department of Microbiology, University of Washington, 98195, Seattle, WA, USA"
],
"type": "Organization"
},
"familyName": "Lacourse",
"givenName": "Kaitlyn D.",
"id": "sg:person.011132153260.47",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011132153260.47"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Architecture et Fonction des Macromolecules Biologiques, Aix-Marseille Universite, CNRS, Campus de Luminy, Case 932, 13288, Marseille, Cedex 09, France",
"id": "http://www.grid.ac/institutes/grid.463764.4",
"name": [
"Architecture et Fonction des Macromolecules Biologiques, Aix-Marseille Universite, CNRS, Campus de Luminy, Case 932, 13288, Marseille, Cedex 09, France"
],
"type": "Organization"
},
"familyName": "Cambillau",
"givenName": "Christian",
"id": "sg:person.01314152236.73",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01314152236.73"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Biochemistry, University of Washington, 98195, Seattle, WA, USA",
"id": "http://www.grid.ac/institutes/grid.34477.33",
"name": [
"Department of Biochemistry, University of Washington, 98195, Seattle, WA, USA"
],
"type": "Organization"
},
"familyName": "DiMaio",
"givenName": "Frank",
"id": "sg:person.0660477641.01",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0660477641.01"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Howard Hughes Medical Institute, University of Washington, 98195, Seattle, WA, USA",
"id": "http://www.grid.ac/institutes/grid.34477.33",
"name": [
"Department of Microbiology, University of Washington, 98195, Seattle, WA, USA",
"Howard Hughes Medical Institute, University of Washington, 98195, Seattle, WA, USA"
],
"type": "Organization"
},
"familyName": "Mougous",
"givenName": "Joseph D.",
"id": "sg:person.01125303721.76",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01125303721.76"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Biochemistry, University of Washington, 98195, Seattle, WA, USA",
"id": "http://www.grid.ac/institutes/grid.34477.33",
"name": [
"Department of Biochemistry, University of Washington, 98195, Seattle, WA, USA"
],
"type": "Organization"
},
"familyName": "Veesler",
"givenName": "David",
"id": "sg:person.01002414777.95",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01002414777.95"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1038/nmicrobiol.2017.103",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1086189894",
"https://doi.org/10.1038/nmicrobiol.2017.103"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nmeth.4169",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1083686092",
"https://doi.org/10.1038/nmeth.4169"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature14667",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010547308",
"https://doi.org/10.1038/nature14667"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/s41564-017-0020-7",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1091910477",
"https://doi.org/10.1038/s41564-017-0020-7"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature10846",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1015413547",
"https://doi.org/10.1038/nature10846"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nmeth.2115",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1041024070",
"https://doi.org/10.1038/nmeth.2115"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nsmb.2995",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1033335278",
"https://doi.org/10.1038/nsmb.2995"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/415553a",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1002785834",
"https://doi.org/10.1038/415553a"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt.2419",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026577191",
"https://doi.org/10.1038/nbt.2419"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/ncomms16088",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1090651781",
"https://doi.org/10.1038/ncomms16088"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nmeth.4340",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1086071524",
"https://doi.org/10.1038/nmeth.4340"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nmeth.3286",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1015110469",
"https://doi.org/10.1038/nmeth.3286"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature12453",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050066013",
"https://doi.org/10.1038/nature12453"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature17971",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1002221590",
"https://doi.org/10.1038/nature17971"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nmeth.4193",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1084129286",
"https://doi.org/10.1038/nmeth.4193"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature17182",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1006559637",
"https://doi.org/10.1038/nature17182"
],
"type": "CreativeWork"
}
],
"datePublished": "2018-12-19",
"datePublishedReg": "2018-12-19",
"description": "Type VI secretion systems (T6SSs) translocate effectors into target cells and are made of a contractile sheath and a tube docked onto a multi-protein transmembrane complex via a baseplate. Although some information is available about the mechanisms of tail contraction leading to effector delivery, the detailed architecture and function of the baseplate remain unknown. Here, we report the 3.7\u2009\u00c5 resolution cryo-electron microscopy reconstruction of an enteroaggregative Escherichia coli baseplate subcomplex assembled from TssK, TssF and TssG. The structure reveals two TssK trimers interact with a locally pseudo-3-fold symmetrical complex comprising two copies of TssF and one copy of TssG. TssF and TssG are structurally related to each other and to components of the phage T4 baseplate and of the type IV secretion system, strengthening the evolutionary relationships among these macromolecular machines. These results, together with bacterial two-hybrid assays, provide a structural framework to understand the T6SS baseplate architecture.",
"genre": "article",
"id": "sg:pub.10.1038/s41467-018-07796-5",
"inLanguage": "en",
"isAccessibleForFree": true,
"isFundedItemOf": [
{
"id": "sg:grant.7027576",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.2458460",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.7169995",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.2683719",
"type": "MonetaryGrant"
}
],
"isPartOf": [
{
"id": "sg:journal.1043282",
"issn": [
"2041-1723"
],
"name": "Nature Communications",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "1",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "9"
}
],
"keywords": [
"secretion system",
"\u00c5 resolution cryo-electron microscopy reconstruction",
"type VI secretion system",
"cryo-electron microscopy reconstruction",
"type IV secretion system",
"two-hybrid assay",
"cryo-electron microscopy",
"macromolecular machines",
"evolutionary relationships",
"effector delivery",
"transmembrane complex",
"T4 baseplate",
"contractile sheath",
"tail contraction",
"subcomplex",
"TssF",
"target cells",
"copies",
"structural framework",
"TssK",
"detailed architecture",
"effectors",
"baseplate",
"cells",
"assays",
"complexes",
"trimer",
"mechanism",
"structure",
"function",
"sheath",
"microscopy",
"TSSG",
"components",
"architecture",
"system",
"relationship",
"information",
"results",
"contraction",
"reconstruction",
"tube",
"delivery",
"framework",
"machine"
],
"name": "Structure of the type VI secretion system TssK\u2013TssF\u2013TssG baseplate subcomplex revealed by cryo-electron microscopy",
"pagination": "5385",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1110584070"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1038/s41467-018-07796-5"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"30568167"
]
}
],
"sameAs": [
"https://doi.org/10.1038/s41467-018-07796-5",
"https://app.dimensions.ai/details/publication/pub.1110584070"
],
"sdDataset": "articles",
"sdDatePublished": "2022-06-01T22:18",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220601/entities/gbq_results/article/article_783.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1038/s41467-018-07796-5"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41467-018-07796-5'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41467-018-07796-5'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41467-018-07796-5'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41467-018-07796-5'
This table displays all metadata directly associated to this object as RDF triples.
244 TRIPLES
22 PREDICATES
93 URIs
69 LITERALS
13 BLANK NODES