Targeting fidelity of adenine and cytosine base editors in mouse embryos View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-12

AUTHORS

Hye Kyung Lee, Michaela Willi, Shannon M. Miller, Sojung Kim, Chengyu Liu, David R. Liu, Lothar Hennighausen

ABSTRACT

Base editing directly converts a target base pair into a different base pair in the genome of living cells without introducing double-stranded DNA breaks. While cytosine base editors (CBE) and adenine base editors (ABE) are used to install and correct point mutations in a wide range of organisms, the extent and distribution of off-target edits in mammalian embryos have not been studied in detail. We analyze on-target and proximal off-target editing at 13 loci by a variety of CBEs and ABE in more than 430 alleles generated from mouse zygotic injections using newly generated and published sequencing data. ABE predominantly generates anticipated A•T-to-G•C edits. Among CBEs, SaBE3 and BE4, result in the highest frequencies of anticipated C•G-to-T•A products relative to editing byproducts. Together, these findings highlight the remarkable fidelity of ABE in mouse embryos and identify preferred CBE variants when fidelity in vivo is critical. More... »

PAGES

4804

References to SciGraph publications

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41467-018-07322-7

    DOI

    http://dx.doi.org/10.1038/s41467-018-07322-7

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1109841472

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30442934


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