Subtomogram averaging of COPII assemblies reveals how coat organization dictates membrane shape View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-10-08

AUTHORS

Joshua Hutchings, Viktoriya Stancheva, Elizabeth A. Miller, Giulia Zanetti

ABSTRACT

Eukaryotic cells employ membrane-bound carriers to transport cargo between compartments in a process essential to cell functionality. Carriers are generated by coat complexes that couple cargo capture to membrane deformation. The COPII coat mediates export from the endoplasmic reticulum by assembling in inner and outer layers, yielding carriers of variable shape and size that allow secretion of thousands of diverse cargo. Despite detailed understanding of COPII subunits, the molecular mechanisms of coat assembly and membrane deformation are unclear. Here we present a 4.9 Å cryo-tomography subtomogram averaging structure of in vitro-reconstituted membrane-bound inner coat. We show that the outer coat (Sec13–Sec31) bridges inner coat subunits (Sar1–Sec23–Sec24), promoting their assembly into a tight lattice. We directly visualize the membrane-embedded Sar1 amphipathic helix, revealing that lattice formation induces parallel helix insertions, yielding tubular curvature. We propose that regulators like the procollagen receptor TANGO1 modulate this mechanism to determine vesicle shape and size. More... »

PAGES

4154

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41467-018-06577-4

DOI

http://dx.doi.org/10.1038/s41467-018-06577-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1107363261

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30297805


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "COP-Coated Vesicles", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Membrane", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cryoelectron Microscopy", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Electron Microscope Tomography", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Endoplasmic Reticulum", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Membrane Transport Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Binding", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Transport", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Saccharomyces cerevisiae Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sf9 Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Spodoptera", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Institute of Structural and Molecular Biology, Birkbeck College, Malet St., WC1E 7HX, London, UK", 
          "id": "http://www.grid.ac/institutes/grid.509978.a", 
          "name": [
            "Institute of Structural and Molecular Biology, Birkbeck College, Malet St., WC1E 7HX, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hutchings", 
        "givenName": "Joshua", 
        "id": "sg:person.07764712606.84", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07764712606.84"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "MRC Laboratory of Molecular Biology, Francis Crick Ave., CB2 0QH, Cambridge, UK", 
          "id": "http://www.grid.ac/institutes/grid.42475.30", 
          "name": [
            "MRC Laboratory of Molecular Biology, Francis Crick Ave., CB2 0QH, Cambridge, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Stancheva", 
        "givenName": "Viktoriya", 
        "id": "sg:person.012565777104.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012565777104.05"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "MRC Laboratory of Molecular Biology, Francis Crick Ave., CB2 0QH, Cambridge, UK", 
          "id": "http://www.grid.ac/institutes/grid.42475.30", 
          "name": [
            "MRC Laboratory of Molecular Biology, Francis Crick Ave., CB2 0QH, Cambridge, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Miller", 
        "givenName": "Elizabeth A.", 
        "id": "sg:person.0627102647.36", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0627102647.36"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Structural and Molecular Biology, Birkbeck College, Malet St., WC1E 7HX, London, UK", 
          "id": "http://www.grid.ac/institutes/grid.509978.a", 
          "name": [
            "Institute of Structural and Molecular Biology, Birkbeck College, Malet St., WC1E 7HX, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zanetti", 
        "givenName": "Giulia", 
        "id": "sg:person.01220007377.23", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01220007377.23"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/35078500", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005889743", 
          "https://doi.org/10.1038/35078500"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.4193", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084129286", 
          "https://doi.org/10.1038/nmeth.4193"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb2390", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042603741", 
          "https://doi.org/10.1038/ncb2390"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature01040", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001233428", 
          "https://doi.org/10.1038/nature01040"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature04396", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019599431", 
          "https://doi.org/10.1038/nature04396"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature04339", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007438875", 
          "https://doi.org/10.1038/nature04339"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/srep00017", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048569722", 
          "https://doi.org/10.1038/srep00017"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2018-10-08", 
    "datePublishedReg": "2018-10-08", 
    "description": "Eukaryotic cells employ membrane-bound carriers to transport cargo between compartments in a process essential to cell functionality. Carriers are generated by coat complexes that couple cargo capture to membrane deformation. The COPII coat mediates export from the endoplasmic reticulum by assembling in inner and outer layers, yielding carriers of variable shape and size that allow secretion of thousands of diverse cargo. Despite detailed understanding of COPII subunits, the molecular mechanisms of coat assembly and membrane deformation are\u00a0unclear. Here we present a 4.9\u2009\u00c5 cryo-tomography subtomogram averaging structure of in vitro-reconstituted membrane-bound inner coat. We show that the outer coat (Sec13\u2013Sec31) bridges inner coat subunits (Sar1\u2013Sec23\u2013Sec24), promoting their assembly into a tight lattice. We directly visualize the membrane-embedded Sar1 amphipathic helix, revealing that lattice formation induces parallel helix insertions, yielding tubular curvature. We propose that regulators like the procollagen receptor TANGO1 modulate this mechanism to determine vesicle shape and size.", 
    "genre": "article", 
    "id": "sg:pub.10.1038/s41467-018-06577-4", 
    "inLanguage": "en", 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.4294432", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.3940318", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.4580642", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1043282", 
        "issn": [
          "2041-1723"
        ], 
        "name": "Nature Communications", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "9"
      }
    ], 
    "keywords": [
      "membrane deformation", 
      "membrane-bound carriers", 
      "COPII assembly", 
      "eukaryotic cells", 
      "coat complex", 
      "COPII coat", 
      "COPII subunits", 
      "coat assembly", 
      "coat subunits", 
      "amphipathic helix", 
      "subtomogram averaging", 
      "helix insertion", 
      "diverse cargoes", 
      "cargo capture", 
      "molecular mechanisms", 
      "endoplasmic reticulum", 
      "membrane shape", 
      "subunits", 
      "cell functionality", 
      "inner coat", 
      "outer coat", 
      "assembly", 
      "cargo", 
      "detailed understanding", 
      "coat", 
      "TANGO1", 
      "vesicle shape", 
      "regulator", 
      "reticulum", 
      "helix", 
      "lattice formation", 
      "mechanism", 
      "compartments", 
      "cells", 
      "complexes", 
      "export", 
      "secretion", 
      "thousands", 
      "insertion", 
      "variable shape", 
      "outer layer", 
      "understanding", 
      "size", 
      "formation", 
      "capture", 
      "structure", 
      "shape", 
      "process", 
      "organization", 
      "carriers", 
      "functionality", 
      "averaging", 
      "layer", 
      "curvature", 
      "lattice", 
      "deformation", 
      "tight lattice", 
      "tubular curvature"
    ], 
    "name": "Subtomogram averaging of COPII assemblies reveals how coat organization dictates membrane shape", 
    "pagination": "4154", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1107363261"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/s41467-018-06577-4"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30297805"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/s41467-018-06577-4", 
      "https://app.dimensions.ai/details/publication/pub.1107363261"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-06-01T22:20", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220601/entities/gbq_results/article/article_794.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1038/s41467-018-06577-4"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41467-018-06577-4'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41467-018-06577-4'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41467-018-06577-4'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41467-018-06577-4'


 

This table displays all metadata directly associated to this object as RDF triples.

225 TRIPLES      22 PREDICATES      103 URIs      88 LITERALS      19 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/s41467-018-06577-4 schema:about N0693c6ed4b154388a5dd8e46d083b209
2 N1298f951ecf14f7f8268b19146f7a447
3 N2cace35ee0644eb3aa14ed710c9de5fb
4 N39d97b0263ae48e3b821ffec4f93ed5a
5 N43d87f14b57e4cd4bd95afe3e45a582e
6 N487abaa6604a44fab47c1ca6eac134a5
7 N5a23c863cf48429d929963f0fb9c0c69
8 N6b3e0b5697d54309a646ccb2f09cc882
9 N91c455036d6b4cbe83cada92f06be312
10 N9a900bc66b434f859b233adcb372b54d
11 Ncfc6d45e95ed4ae5a7de6c208d8ae985
12 Nd8788b4df0a049838f991003f64f5b5f
13 anzsrc-for:06
14 anzsrc-for:0601
15 schema:author N432639a8a8a74dc1aefc5ec71afe4ead
16 schema:citation sg:pub.10.1038/35078500
17 sg:pub.10.1038/nature01040
18 sg:pub.10.1038/nature04339
19 sg:pub.10.1038/nature04396
20 sg:pub.10.1038/ncb2390
21 sg:pub.10.1038/nmeth.4193
22 sg:pub.10.1038/srep00017
23 schema:datePublished 2018-10-08
24 schema:datePublishedReg 2018-10-08
25 schema:description Eukaryotic cells employ membrane-bound carriers to transport cargo between compartments in a process essential to cell functionality. Carriers are generated by coat complexes that couple cargo capture to membrane deformation. The COPII coat mediates export from the endoplasmic reticulum by assembling in inner and outer layers, yielding carriers of variable shape and size that allow secretion of thousands of diverse cargo. Despite detailed understanding of COPII subunits, the molecular mechanisms of coat assembly and membrane deformation are unclear. Here we present a 4.9 Å cryo-tomography subtomogram averaging structure of in vitro-reconstituted membrane-bound inner coat. We show that the outer coat (Sec13–Sec31) bridges inner coat subunits (Sar1–Sec23–Sec24), promoting their assembly into a tight lattice. We directly visualize the membrane-embedded Sar1 amphipathic helix, revealing that lattice formation induces parallel helix insertions, yielding tubular curvature. We propose that regulators like the procollagen receptor TANGO1 modulate this mechanism to determine vesicle shape and size.
26 schema:genre article
27 schema:inLanguage en
28 schema:isAccessibleForFree true
29 schema:isPartOf Nda98cef3588f485f8e08faeaaf1047f4
30 Nff77b3fe0f8b49d59c72ed2770e4c443
31 sg:journal.1043282
32 schema:keywords COPII assembly
33 COPII coat
34 COPII subunits
35 TANGO1
36 amphipathic helix
37 assembly
38 averaging
39 capture
40 cargo
41 cargo capture
42 carriers
43 cell functionality
44 cells
45 coat
46 coat assembly
47 coat complex
48 coat subunits
49 compartments
50 complexes
51 curvature
52 deformation
53 detailed understanding
54 diverse cargoes
55 endoplasmic reticulum
56 eukaryotic cells
57 export
58 formation
59 functionality
60 helix
61 helix insertion
62 inner coat
63 insertion
64 lattice
65 lattice formation
66 layer
67 mechanism
68 membrane deformation
69 membrane shape
70 membrane-bound carriers
71 molecular mechanisms
72 organization
73 outer coat
74 outer layer
75 process
76 regulator
77 reticulum
78 secretion
79 shape
80 size
81 structure
82 subtomogram averaging
83 subunits
84 thousands
85 tight lattice
86 tubular curvature
87 understanding
88 variable shape
89 vesicle shape
90 schema:name Subtomogram averaging of COPII assemblies reveals how coat organization dictates membrane shape
91 schema:pagination 4154
92 schema:productId N2551ed0b3b444ddfa0d6f4253f28d3bc
93 N53d37099dc97468c9f0f14501b22c81a
94 N6bf37b54381a4680a9fcc470a307e3d8
95 schema:sameAs https://app.dimensions.ai/details/publication/pub.1107363261
96 https://doi.org/10.1038/s41467-018-06577-4
97 schema:sdDatePublished 2022-06-01T22:20
98 schema:sdLicense https://scigraph.springernature.com/explorer/license/
99 schema:sdPublisher N4b854c4e42d04a3cace87280b6ace5c1
100 schema:url https://doi.org/10.1038/s41467-018-06577-4
101 sgo:license sg:explorer/license/
102 sgo:sdDataset articles
103 rdf:type schema:ScholarlyArticle
104 N027dc1a4e1374e0681242b3dd4ebedec rdf:first sg:person.012565777104.05
105 rdf:rest N5922df2ff79c46b891d1cc6d81a085ea
106 N0693c6ed4b154388a5dd8e46d083b209 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Sf9 Cells
108 rdf:type schema:DefinedTerm
109 N1298f951ecf14f7f8268b19146f7a447 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
110 schema:name Electron Microscope Tomography
111 rdf:type schema:DefinedTerm
112 N2551ed0b3b444ddfa0d6f4253f28d3bc schema:name dimensions_id
113 schema:value pub.1107363261
114 rdf:type schema:PropertyValue
115 N2cace35ee0644eb3aa14ed710c9de5fb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Spodoptera
117 rdf:type schema:DefinedTerm
118 N39d97b0263ae48e3b821ffec4f93ed5a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name COP-Coated Vesicles
120 rdf:type schema:DefinedTerm
121 N432639a8a8a74dc1aefc5ec71afe4ead rdf:first sg:person.07764712606.84
122 rdf:rest N027dc1a4e1374e0681242b3dd4ebedec
123 N43d87f14b57e4cd4bd95afe3e45a582e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Cell Membrane
125 rdf:type schema:DefinedTerm
126 N487abaa6604a44fab47c1ca6eac134a5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Membrane Transport Proteins
128 rdf:type schema:DefinedTerm
129 N4b854c4e42d04a3cace87280b6ace5c1 schema:name Springer Nature - SN SciGraph project
130 rdf:type schema:Organization
131 N53d37099dc97468c9f0f14501b22c81a schema:name doi
132 schema:value 10.1038/s41467-018-06577-4
133 rdf:type schema:PropertyValue
134 N5922df2ff79c46b891d1cc6d81a085ea rdf:first sg:person.0627102647.36
135 rdf:rest Nbb9bf2a6cb8a4f478cebbf79716220cf
136 N5a23c863cf48429d929963f0fb9c0c69 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Protein Transport
138 rdf:type schema:DefinedTerm
139 N6b3e0b5697d54309a646ccb2f09cc882 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Saccharomyces cerevisiae Proteins
141 rdf:type schema:DefinedTerm
142 N6bf37b54381a4680a9fcc470a307e3d8 schema:name pubmed_id
143 schema:value 30297805
144 rdf:type schema:PropertyValue
145 N91c455036d6b4cbe83cada92f06be312 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name Animals
147 rdf:type schema:DefinedTerm
148 N9a900bc66b434f859b233adcb372b54d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
149 schema:name Cryoelectron Microscopy
150 rdf:type schema:DefinedTerm
151 Nbb9bf2a6cb8a4f478cebbf79716220cf rdf:first sg:person.01220007377.23
152 rdf:rest rdf:nil
153 Ncfc6d45e95ed4ae5a7de6c208d8ae985 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
154 schema:name Protein Binding
155 rdf:type schema:DefinedTerm
156 Nd8788b4df0a049838f991003f64f5b5f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
157 schema:name Endoplasmic Reticulum
158 rdf:type schema:DefinedTerm
159 Nda98cef3588f485f8e08faeaaf1047f4 schema:issueNumber 1
160 rdf:type schema:PublicationIssue
161 Nff77b3fe0f8b49d59c72ed2770e4c443 schema:volumeNumber 9
162 rdf:type schema:PublicationVolume
163 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
164 schema:name Biological Sciences
165 rdf:type schema:DefinedTerm
166 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
167 schema:name Biochemistry and Cell Biology
168 rdf:type schema:DefinedTerm
169 sg:grant.3940318 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-018-06577-4
170 rdf:type schema:MonetaryGrant
171 sg:grant.4294432 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-018-06577-4
172 rdf:type schema:MonetaryGrant
173 sg:grant.4580642 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-018-06577-4
174 rdf:type schema:MonetaryGrant
175 sg:journal.1043282 schema:issn 2041-1723
176 schema:name Nature Communications
177 schema:publisher Springer Nature
178 rdf:type schema:Periodical
179 sg:person.01220007377.23 schema:affiliation grid-institutes:grid.509978.a
180 schema:familyName Zanetti
181 schema:givenName Giulia
182 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01220007377.23
183 rdf:type schema:Person
184 sg:person.012565777104.05 schema:affiliation grid-institutes:grid.42475.30
185 schema:familyName Stancheva
186 schema:givenName Viktoriya
187 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012565777104.05
188 rdf:type schema:Person
189 sg:person.0627102647.36 schema:affiliation grid-institutes:grid.42475.30
190 schema:familyName Miller
191 schema:givenName Elizabeth A.
192 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0627102647.36
193 rdf:type schema:Person
194 sg:person.07764712606.84 schema:affiliation grid-institutes:grid.509978.a
195 schema:familyName Hutchings
196 schema:givenName Joshua
197 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07764712606.84
198 rdf:type schema:Person
199 sg:pub.10.1038/35078500 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005889743
200 https://doi.org/10.1038/35078500
201 rdf:type schema:CreativeWork
202 sg:pub.10.1038/nature01040 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001233428
203 https://doi.org/10.1038/nature01040
204 rdf:type schema:CreativeWork
205 sg:pub.10.1038/nature04339 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007438875
206 https://doi.org/10.1038/nature04339
207 rdf:type schema:CreativeWork
208 sg:pub.10.1038/nature04396 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019599431
209 https://doi.org/10.1038/nature04396
210 rdf:type schema:CreativeWork
211 sg:pub.10.1038/ncb2390 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042603741
212 https://doi.org/10.1038/ncb2390
213 rdf:type schema:CreativeWork
214 sg:pub.10.1038/nmeth.4193 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084129286
215 https://doi.org/10.1038/nmeth.4193
216 rdf:type schema:CreativeWork
217 sg:pub.10.1038/srep00017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048569722
218 https://doi.org/10.1038/srep00017
219 rdf:type schema:CreativeWork
220 grid-institutes:grid.42475.30 schema:alternateName MRC Laboratory of Molecular Biology, Francis Crick Ave., CB2 0QH, Cambridge, UK
221 schema:name MRC Laboratory of Molecular Biology, Francis Crick Ave., CB2 0QH, Cambridge, UK
222 rdf:type schema:Organization
223 grid-institutes:grid.509978.a schema:alternateName Institute of Structural and Molecular Biology, Birkbeck College, Malet St., WC1E 7HX, London, UK
224 schema:name Institute of Structural and Molecular Biology, Birkbeck College, Malet St., WC1E 7HX, London, UK
225 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...