An efficient and multiple target transgenic RNAi technique with low toxicity in Drosophila View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-12

AUTHORS

Huan-Huan Qiao, Fang Wang, Rong-Gang Xu, Jin Sun, Ruibao Zhu, Decai Mao, Xingjie Ren, Xia Wang, Yu Jia, Ping Peng, Da Shen, Lu-Ping Liu, Zhijie Chang, Guirong Wang, Shao Li, Jun-Yuan Ji, Qingfei Liu, Jian-Quan Ni

ABSTRACT

Being relatively simple and practical, Drosophila transgenic RNAi is the technique of top priority choice to quickly study genes with pleiotropic functions. However, drawbacks have emerged over time, such as high level of false positive and negative results. To overcome these shortcomings and increase efficiency, specificity and versatility, we develop a next generation transgenic RNAi system. With this system, the leaky expression of the basal promoter is significantly reduced, as well as the heterozygous ratio of transgenic RNAi flies. In addition, it has been first achieved to precisely and efficiently modulate highly expressed genes. Furthermore, we increase versatility which can simultaneously knock down multiple genes in one step. A case illustration is provided of how this system can be used to study the synthetic developmental effect of histone acetyltransferases. Finally, we have generated a collection of transgenic RNAi lines for those genes that are highly homologous to human disease genes. More... »

PAGES

4160

References to SciGraph publications

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41467-018-06537-y

    DOI

    http://dx.doi.org/10.1038/s41467-018-06537-y

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1107363256

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30297884


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