Vibrio parahaemolyticus RhsP represents a widespread group of pro-effectors for type VI secretion systems View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-09-25

AUTHORS

Nan Jiang, Le Tang, Ruiqiang Xie, Zhi Li, Brianne Burkinshaw, Xiaoye Liang, Dylan Sosa, L. Aravind, Tao Dong, Dapeng Zhang, Jun Zheng

ABSTRACT

Type VI secretion systems (T6SSs) translocate effector proteins, such as Rhs toxins, to eukaryotic cells or prokaryotic competitors. All T6SS Rhs-type effectors characterized thus far contain a PAAR motif or a similar structure. Here, we describe a T6SS-dependent delivery mechanism for a subset of Rhs proteins that lack a PAAR motif. We show that the N-terminal Rhs domain of protein RhsP (or VP1517) from Vibrio parahaemolyticus inhibits the activity of the C-terminal DNase domain. Upon auto-proteolysis, the Rhs fragment remains inside the cells, and the C-terminal region interacts with PAAR2 and is secreted by T6SS2; therefore, RhsP acts as a pro-effector. Furthermore, we show that RhsP contributes to the control of certain "social cheaters" (opaR mutants). Genes encoding proteins with similar Rhs and PAAR-interacting domains, but diverse C-terminal regions, are widely distributed among Vibrio species. More... »

PAGES

3899

References to SciGraph publications

  • 2012-04-26. Putative type VI secretion systems of Vibrio parahaemolyticus contribute to adhesion to cultured cell monolayers in ARCHIVES OF MICROBIOLOGY
  • 2018-04-09. A type VI secretion system effector delivery mechanism dependent on PAAR and a chaperone–co-chaperone complex in NATURE MICROBIOLOGY
  • 2014-01-02. Type VI secretion system effectors: poisons with a purpose in NATURE REVIEWS MICROBIOLOGY
  • 1997-04. Activation of prokaryotic transcription through arbitrary protein–protein contacts in NATURE
  • 2012-06-25. Polymorphic toxin systems: Comprehensive characterization of trafficking modes, processing, mechanisms of action, immunity and ecology using comparative genomics in BIOLOGY DIRECT
  • 2009-11-30. Bacterial competition: surviving and thriving in the microbial jungle in NATURE REVIEWS MICROBIOLOGY
  • 2014-02-23. Mechanism of Tc toxin action revealed in molecular detail in NATURE
  • 2005-12-12. Kalign – an accurate and fast multiple sequence alignment algorithm in BMC BIOINFORMATICS
  • 2015-05-07. The Phyre2 web portal for protein modeling, prediction and analysis in NATURE PROTOCOLS
  • 2004-08-19. MUSCLE: a multiple sequence alignment method with reduced time and space complexity in BMC BIOINFORMATICS
  • 2012-02-26. Type VI secretion requires a dynamic contractile phage tail-like structure in NATURE
  • 2015-05-15. Secretion systems in Gram-negative bacteria: structural and mechanistic insights in NATURE REVIEWS MICROBIOLOGY
  • 2013-08-04. The BC component of ABC toxins is an RHS-repeat-containing protein encapsulation device in NATURE
  • 2017-03-28. A Pseudomonas T6SS effector recruits PQS-containing outer membrane vesicles for iron acquisition in NATURE COMMUNICATIONS
  • 2013-08-07. PAAR-repeat proteins sharpen and diversify the Type VI secretion system spike in NATURE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41467-018-06201-5

    DOI

    http://dx.doi.org/10.1038/s41467-018-06201-5

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1107107974

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30254227


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Amino Acid Motifs", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Amino Acid Sequence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Bacterial Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Bacterial Toxins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Binding Sites", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mutagenesis, Site-Directed", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Protein Binding", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Protein Domains", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Sequence Homology, Amino Acid", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Type VI Secretion Systems", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Vibrio parahaemolyticus", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Faculty of Health Sciences, University of Macau, Macau SAR, China", 
              "id": "http://www.grid.ac/institutes/grid.437123.0", 
              "name": [
                "Faculty of Health Sciences, University of Macau, Macau SAR, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Jiang", 
            "givenName": "Nan", 
            "id": "sg:person.015376132277.67", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015376132277.67"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Snyder Institute for Chronic Diseases, University of Calgary, Calgary, AB T2N 4Z6 Canada", 
              "id": "http://www.grid.ac/institutes/grid.22072.35", 
              "name": [
                "Department of Ecosystem and Public Health, University of Calgary, Calgary, AB T2N 4Z6 Canada", 
                "Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, AB T2N 4Z6 Canada", 
                "Snyder Institute for Chronic Diseases, University of Calgary, Calgary, AB T2N 4Z6 Canada"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Tang", 
            "givenName": "Le", 
            "id": "sg:person.01057370437.27", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01057370437.27"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Faculty of Health Sciences, University of Macau, Macau SAR, China", 
              "id": "http://www.grid.ac/institutes/grid.437123.0", 
              "name": [
                "Faculty of Health Sciences, University of Macau, Macau SAR, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Xie", 
            "givenName": "Ruiqiang", 
            "id": "sg:person.016242423704.83", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016242423704.83"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Faculty of Health Sciences, University of Macau, Macau SAR, China", 
              "id": "http://www.grid.ac/institutes/grid.437123.0", 
              "name": [
                "Faculty of Health Sciences, University of Macau, Macau SAR, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Li", 
            "givenName": "Zhi", 
            "id": "sg:person.015743462756.15", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015743462756.15"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Snyder Institute for Chronic Diseases, University of Calgary, Calgary, AB T2N 4Z6 Canada", 
              "id": "http://www.grid.ac/institutes/grid.22072.35", 
              "name": [
                "Department of Ecosystem and Public Health, University of Calgary, Calgary, AB T2N 4Z6 Canada", 
                "Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, AB T2N 4Z6 Canada", 
                "Snyder Institute for Chronic Diseases, University of Calgary, Calgary, AB T2N 4Z6 Canada"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Burkinshaw", 
            "givenName": "Brianne", 
            "id": "sg:person.0601156616.50", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0601156616.50"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "State Key Laboratory of Microbial Metabolism, Joint International Laboratory on Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240 China", 
              "id": "http://www.grid.ac/institutes/grid.16821.3c", 
              "name": [
                "State Key Laboratory of Microbial Metabolism, Joint International Laboratory on Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240 China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Liang", 
            "givenName": "Xiaoye", 
            "id": "sg:person.01261456402.66", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01261456402.66"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Program of Bioinformatics and Computational Biology, College of Arts and Sciences, Saint Louis University, Saint Louis, MO 63103 USA", 
              "id": "http://www.grid.ac/institutes/grid.262962.b", 
              "name": [
                "Program of Bioinformatics and Computational Biology, College of Arts and Sciences, Saint Louis University, Saint Louis, MO 63103 USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Sosa", 
            "givenName": "Dylan", 
            "id": "sg:person.010102132270.10", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010102132270.10"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894 USA", 
              "id": "http://www.grid.ac/institutes/grid.419234.9", 
              "name": [
                "National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894 USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Aravind", 
            "givenName": "L.", 
            "id": "sg:person.01106662166.38", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01106662166.38"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "State Key Laboratory of Microbial Metabolism, Joint International Laboratory on Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240 China", 
              "id": "http://www.grid.ac/institutes/grid.16821.3c", 
              "name": [
                "Department of Ecosystem and Public Health, University of Calgary, Calgary, AB T2N 4Z6 Canada", 
                "Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, AB T2N 4Z6 Canada", 
                "Snyder Institute for Chronic Diseases, University of Calgary, Calgary, AB T2N 4Z6 Canada", 
                "State Key Laboratory of Microbial Metabolism, Joint International Laboratory on Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240 China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Dong", 
            "givenName": "Tao", 
            "id": "sg:person.01060105505.22", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01060105505.22"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Biology, College of Arts and Sciences, Saint Louis University, Saint Louis, MO 63103 USA", 
              "id": "http://www.grid.ac/institutes/grid.262962.b", 
              "name": [
                "Program of Bioinformatics and Computational Biology, College of Arts and Sciences, Saint Louis University, Saint Louis, MO 63103 USA", 
                "Department of Biology, College of Arts and Sciences, Saint Louis University, Saint Louis, MO 63103 USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zhang", 
            "givenName": "Dapeng", 
            "id": "sg:person.0641126326.37", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0641126326.37"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Faculty of Health Sciences, University of Macau, Macau SAR, China", 
              "id": "http://www.grid.ac/institutes/grid.437123.0", 
              "name": [
                "Faculty of Health Sciences, University of Macau, Macau SAR, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zheng", 
            "givenName": "Jun", 
            "id": "sg:person.010013737675.04", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010013737675.04"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/nature13015", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050163881", 
              "https://doi.org/10.1038/nature13015"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1471-2105-5-113", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040413794", 
              "https://doi.org/10.1186/1471-2105-5-113"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1745-6150-7-18", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040385591", 
              "https://doi.org/10.1186/1745-6150-7-18"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrmicro3456", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043059122", 
              "https://doi.org/10.1038/nrmicro3456"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00203-012-0816-z", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026405140", 
              "https://doi.org/10.1007/s00203-012-0816-z"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature12465", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025304440", 
              "https://doi.org/10.1038/nature12465"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/386627a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036416155", 
              "https://doi.org/10.1038/386627a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ncomms14888", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1084129063", 
              "https://doi.org/10.1038/ncomms14888"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/s41564-018-0144-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1103192448", 
              "https://doi.org/10.1038/s41564-018-0144-4"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrmicro3185", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024058313", 
              "https://doi.org/10.1038/nrmicro3185"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrmicro2259", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036917778", 
              "https://doi.org/10.1038/nrmicro2259"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature12453", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050066013", 
              "https://doi.org/10.1038/nature12453"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1471-2105-6-298", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047546418", 
              "https://doi.org/10.1186/1471-2105-6-298"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nprot.2015.053", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001304490", 
              "https://doi.org/10.1038/nprot.2015.053"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature10846", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015413547", 
              "https://doi.org/10.1038/nature10846"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2018-09-25", 
        "datePublishedReg": "2018-09-25", 
        "description": "Type VI secretion systems (T6SSs) translocate effector proteins, such as Rhs toxins, to eukaryotic cells or prokaryotic competitors. All T6SS Rhs-type effectors characterized thus far contain a PAAR motif or a similar structure. Here, we describe a T6SS-dependent delivery mechanism for a subset of Rhs proteins that lack a PAAR motif. We show that the N-terminal Rhs domain of protein RhsP (or VP1517) from Vibrio parahaemolyticus inhibits the activity of the C-terminal DNase domain. Upon auto-proteolysis, the Rhs fragment remains inside the cells, and the C-terminal region interacts with PAAR2 and is secreted by T6SS2; therefore, RhsP acts as a pro-effector. Furthermore, we show that RhsP contributes to the control of certain \"social cheaters\" (opaR mutants). Genes encoding proteins with similar Rhs and PAAR-interacting domains, but diverse C-terminal regions, are widely distributed among Vibrio species.", 
        "genre": "article", 
        "id": "sg:pub.10.1038/s41467-018-06201-5", 
        "inLanguage": "en", 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.9339058", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1043282", 
            "issn": [
              "2041-1723"
            ], 
            "name": "Nature Communications", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "9"
          }
        ], 
        "keywords": [
          "terminal region", 
          "type VI secretion system", 
          "terminal DNase domain", 
          "translocate effector proteins", 
          "prokaryotic competitors", 
          "eukaryotic cells", 
          "effector proteins", 
          "social cheaters", 
          "Rh proteins", 
          "secretion system", 
          "RH domain", 
          "DNase domain", 
          "widespread group", 
          "Vibrio species", 
          "protein", 
          "motif", 
          "domain", 
          "T6SS2", 
          "cells", 
          "genes", 
          "species", 
          "effectors", 
          "Vibrio", 
          "similar structure", 
          "rhSP", 
          "cheaters", 
          "fragments", 
          "region", 
          "inhibits", 
          "toxin", 
          "mechanism", 
          "competitors", 
          "activity", 
          "subset", 
          "delivery mechanism", 
          "structure", 
          "control", 
          "system", 
          "group", 
          "Rh", 
          "Type VI secretion systems (T6SSs) translocate effector proteins", 
          "VI secretion systems (T6SSs) translocate effector proteins", 
          "secretion systems (T6SSs) translocate effector proteins", 
          "systems (T6SSs) translocate effector proteins", 
          "Rhs toxins", 
          "T6SS Rhs-type effectors", 
          "Rhs-type effectors", 
          "PAAR motif", 
          "T6SS-dependent delivery mechanism", 
          "terminal Rhs domain", 
          "protein RhsP", 
          "Rh fragments", 
          "PAAR2", 
          "similar Rh", 
          "PAAR-interacting domains", 
          "Vibrio parahaemolyticus RhsP", 
          "parahaemolyticus RhsP", 
          "VI secretion systems"
        ], 
        "name": "Vibrio parahaemolyticus RhsP represents a widespread group of pro-effectors for type VI secretion systems", 
        "pagination": "3899", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1107107974"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/s41467-018-06201-5"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "30254227"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/s41467-018-06201-5", 
          "https://app.dimensions.ai/details/publication/pub.1107107974"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2021-12-01T19:41", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20211201/entities/gbq_results/article/article_758.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1038/s41467-018-06201-5"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41467-018-06201-5'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41467-018-06201-5'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41467-018-06201-5'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41467-018-06201-5'


     

    This table displays all metadata directly associated to this object as RDF triples.

    314 TRIPLES      22 PREDICATES      110 URIs      87 LITERALS      18 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/s41467-018-06201-5 schema:about N180364b4a0b7400592bf010564fa3aa2
    2 N1c41cc9af0b849babb849c8a94ebc647
    3 N2222360937fa41fbbbe7416d0fd9c82d
    4 N335f4129e24b4dab98f14867b72c297f
    5 N3da1cfbe35984ba2a957dca6c157c33c
    6 N63adf0597c89462aa07efc88b2999f1c
    7 N86098235f233487b9264ae09542cae68
    8 N87de4ea99a524358842a04d4da204469
    9 N9086840d9dbf41ffbadcaa37b804a2e2
    10 Nbe2f184c334e49548cc98c513ed4b1f3
    11 Ne4abe7267c924c9cbf7420d70266a8ff
    12 anzsrc-for:06
    13 anzsrc-for:0601
    14 schema:author Nae25b11e384b4bdea1f478cd00e6bf95
    15 schema:citation sg:pub.10.1007/s00203-012-0816-z
    16 sg:pub.10.1038/386627a0
    17 sg:pub.10.1038/nature10846
    18 sg:pub.10.1038/nature12453
    19 sg:pub.10.1038/nature12465
    20 sg:pub.10.1038/nature13015
    21 sg:pub.10.1038/ncomms14888
    22 sg:pub.10.1038/nprot.2015.053
    23 sg:pub.10.1038/nrmicro2259
    24 sg:pub.10.1038/nrmicro3185
    25 sg:pub.10.1038/nrmicro3456
    26 sg:pub.10.1038/s41564-018-0144-4
    27 sg:pub.10.1186/1471-2105-5-113
    28 sg:pub.10.1186/1471-2105-6-298
    29 sg:pub.10.1186/1745-6150-7-18
    30 schema:datePublished 2018-09-25
    31 schema:datePublishedReg 2018-09-25
    32 schema:description Type VI secretion systems (T6SSs) translocate effector proteins, such as Rhs toxins, to eukaryotic cells or prokaryotic competitors. All T6SS Rhs-type effectors characterized thus far contain a PAAR motif or a similar structure. Here, we describe a T6SS-dependent delivery mechanism for a subset of Rhs proteins that lack a PAAR motif. We show that the N-terminal Rhs domain of protein RhsP (or VP1517) from Vibrio parahaemolyticus inhibits the activity of the C-terminal DNase domain. Upon auto-proteolysis, the Rhs fragment remains inside the cells, and the C-terminal region interacts with PAAR2 and is secreted by T6SS2; therefore, RhsP acts as a pro-effector. Furthermore, we show that RhsP contributes to the control of certain "social cheaters" (opaR mutants). Genes encoding proteins with similar Rhs and PAAR-interacting domains, but diverse C-terminal regions, are widely distributed among Vibrio species.
    33 schema:genre article
    34 schema:inLanguage en
    35 schema:isAccessibleForFree true
    36 schema:isPartOf N29377fc07798419ebd90ec00b3593ba0
    37 N916a3f8bf1474b82a7c63b8248efef4c
    38 sg:journal.1043282
    39 schema:keywords DNase domain
    40 PAAR motif
    41 PAAR-interacting domains
    42 PAAR2
    43 RH domain
    44 Rh
    45 Rh fragments
    46 Rh proteins
    47 Rhs toxins
    48 Rhs-type effectors
    49 T6SS Rhs-type effectors
    50 T6SS-dependent delivery mechanism
    51 T6SS2
    52 Type VI secretion systems (T6SSs) translocate effector proteins
    53 VI secretion systems
    54 VI secretion systems (T6SSs) translocate effector proteins
    55 Vibrio
    56 Vibrio parahaemolyticus RhsP
    57 Vibrio species
    58 activity
    59 cells
    60 cheaters
    61 competitors
    62 control
    63 delivery mechanism
    64 domain
    65 effector proteins
    66 effectors
    67 eukaryotic cells
    68 fragments
    69 genes
    70 group
    71 inhibits
    72 mechanism
    73 motif
    74 parahaemolyticus RhsP
    75 prokaryotic competitors
    76 protein
    77 protein RhsP
    78 region
    79 rhSP
    80 secretion system
    81 secretion systems (T6SSs) translocate effector proteins
    82 similar Rh
    83 similar structure
    84 social cheaters
    85 species
    86 structure
    87 subset
    88 system
    89 systems (T6SSs) translocate effector proteins
    90 terminal DNase domain
    91 terminal Rhs domain
    92 terminal region
    93 toxin
    94 translocate effector proteins
    95 type VI secretion system
    96 widespread group
    97 schema:name Vibrio parahaemolyticus RhsP represents a widespread group of pro-effectors for type VI secretion systems
    98 schema:pagination 3899
    99 schema:productId N2f46ed185be1438d8f44060c0842ec18
    100 N45ee14e048c84103bf9e60da153d252f
    101 N4a496edeb7264b77aec360cca7be565c
    102 schema:sameAs https://app.dimensions.ai/details/publication/pub.1107107974
    103 https://doi.org/10.1038/s41467-018-06201-5
    104 schema:sdDatePublished 2021-12-01T19:41
    105 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    106 schema:sdPublisher N4c2f66266b094383848d90a8553fb9f2
    107 schema:url https://doi.org/10.1038/s41467-018-06201-5
    108 sgo:license sg:explorer/license/
    109 sgo:sdDataset articles
    110 rdf:type schema:ScholarlyArticle
    111 N10aa7d1e2aab4838b9ba4a81bc2068c5 rdf:first sg:person.015743462756.15
    112 rdf:rest N8eb3861fe3f84725b3372943d0f8cbc7
    113 N180364b4a0b7400592bf010564fa3aa2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    114 schema:name Amino Acid Sequence
    115 rdf:type schema:DefinedTerm
    116 N1c41cc9af0b849babb849c8a94ebc647 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    117 schema:name Bacterial Proteins
    118 rdf:type schema:DefinedTerm
    119 N2222360937fa41fbbbe7416d0fd9c82d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    120 schema:name Sequence Homology, Amino Acid
    121 rdf:type schema:DefinedTerm
    122 N270903bc3cba41c0892de2f17f41c5c7 rdf:first sg:person.01060105505.22
    123 rdf:rest Ncc35502739ab48bda347461929b6c630
    124 N281615aa67d74622a474561442e7da1c rdf:first sg:person.010102132270.10
    125 rdf:rest N3efd18f61b6146618fde7a34dafe4e7b
    126 N29377fc07798419ebd90ec00b3593ba0 schema:volumeNumber 9
    127 rdf:type schema:PublicationVolume
    128 N2f46ed185be1438d8f44060c0842ec18 schema:name dimensions_id
    129 schema:value pub.1107107974
    130 rdf:type schema:PropertyValue
    131 N335f4129e24b4dab98f14867b72c297f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    132 schema:name Bacterial Toxins
    133 rdf:type schema:DefinedTerm
    134 N3da1cfbe35984ba2a957dca6c157c33c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    135 schema:name Binding Sites
    136 rdf:type schema:DefinedTerm
    137 N3efd18f61b6146618fde7a34dafe4e7b rdf:first sg:person.01106662166.38
    138 rdf:rest N270903bc3cba41c0892de2f17f41c5c7
    139 N45ee14e048c84103bf9e60da153d252f schema:name pubmed_id
    140 schema:value 30254227
    141 rdf:type schema:PropertyValue
    142 N4a496edeb7264b77aec360cca7be565c schema:name doi
    143 schema:value 10.1038/s41467-018-06201-5
    144 rdf:type schema:PropertyValue
    145 N4c2f66266b094383848d90a8553fb9f2 schema:name Springer Nature - SN SciGraph project
    146 rdf:type schema:Organization
    147 N63adf0597c89462aa07efc88b2999f1c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    148 schema:name Vibrio parahaemolyticus
    149 rdf:type schema:DefinedTerm
    150 N814c5fad6efa445986a610edf0756d80 rdf:first sg:person.016242423704.83
    151 rdf:rest N10aa7d1e2aab4838b9ba4a81bc2068c5
    152 N86098235f233487b9264ae09542cae68 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    153 schema:name Protein Domains
    154 rdf:type schema:DefinedTerm
    155 N87de4ea99a524358842a04d4da204469 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    156 schema:name Type VI Secretion Systems
    157 rdf:type schema:DefinedTerm
    158 N8eb3861fe3f84725b3372943d0f8cbc7 rdf:first sg:person.0601156616.50
    159 rdf:rest Nc89cd66b8a774810827a978dd05e4f6a
    160 N9086840d9dbf41ffbadcaa37b804a2e2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    161 schema:name Amino Acid Motifs
    162 rdf:type schema:DefinedTerm
    163 N916a3f8bf1474b82a7c63b8248efef4c schema:issueNumber 1
    164 rdf:type schema:PublicationIssue
    165 Nae25b11e384b4bdea1f478cd00e6bf95 rdf:first sg:person.015376132277.67
    166 rdf:rest Nd99965d2bea64ef8a887287b591b6fab
    167 Nbe2f184c334e49548cc98c513ed4b1f3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    168 schema:name Protein Binding
    169 rdf:type schema:DefinedTerm
    170 Nc89cd66b8a774810827a978dd05e4f6a rdf:first sg:person.01261456402.66
    171 rdf:rest N281615aa67d74622a474561442e7da1c
    172 Ncbb76d4311c74731bb1d356b6c1d0b2a rdf:first sg:person.010013737675.04
    173 rdf:rest rdf:nil
    174 Ncc35502739ab48bda347461929b6c630 rdf:first sg:person.0641126326.37
    175 rdf:rest Ncbb76d4311c74731bb1d356b6c1d0b2a
    176 Nd99965d2bea64ef8a887287b591b6fab rdf:first sg:person.01057370437.27
    177 rdf:rest N814c5fad6efa445986a610edf0756d80
    178 Ne4abe7267c924c9cbf7420d70266a8ff schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    179 schema:name Mutagenesis, Site-Directed
    180 rdf:type schema:DefinedTerm
    181 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    182 schema:name Biological Sciences
    183 rdf:type schema:DefinedTerm
    184 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    185 schema:name Biochemistry and Cell Biology
    186 rdf:type schema:DefinedTerm
    187 sg:grant.9339058 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-018-06201-5
    188 rdf:type schema:MonetaryGrant
    189 sg:journal.1043282 schema:issn 2041-1723
    190 schema:name Nature Communications
    191 schema:publisher Springer Nature
    192 rdf:type schema:Periodical
    193 sg:person.010013737675.04 schema:affiliation grid-institutes:grid.437123.0
    194 schema:familyName Zheng
    195 schema:givenName Jun
    196 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010013737675.04
    197 rdf:type schema:Person
    198 sg:person.010102132270.10 schema:affiliation grid-institutes:grid.262962.b
    199 schema:familyName Sosa
    200 schema:givenName Dylan
    201 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010102132270.10
    202 rdf:type schema:Person
    203 sg:person.01057370437.27 schema:affiliation grid-institutes:grid.22072.35
    204 schema:familyName Tang
    205 schema:givenName Le
    206 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01057370437.27
    207 rdf:type schema:Person
    208 sg:person.01060105505.22 schema:affiliation grid-institutes:grid.16821.3c
    209 schema:familyName Dong
    210 schema:givenName Tao
    211 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01060105505.22
    212 rdf:type schema:Person
    213 sg:person.01106662166.38 schema:affiliation grid-institutes:grid.419234.9
    214 schema:familyName Aravind
    215 schema:givenName L.
    216 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01106662166.38
    217 rdf:type schema:Person
    218 sg:person.01261456402.66 schema:affiliation grid-institutes:grid.16821.3c
    219 schema:familyName Liang
    220 schema:givenName Xiaoye
    221 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01261456402.66
    222 rdf:type schema:Person
    223 sg:person.015376132277.67 schema:affiliation grid-institutes:grid.437123.0
    224 schema:familyName Jiang
    225 schema:givenName Nan
    226 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015376132277.67
    227 rdf:type schema:Person
    228 sg:person.015743462756.15 schema:affiliation grid-institutes:grid.437123.0
    229 schema:familyName Li
    230 schema:givenName Zhi
    231 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015743462756.15
    232 rdf:type schema:Person
    233 sg:person.016242423704.83 schema:affiliation grid-institutes:grid.437123.0
    234 schema:familyName Xie
    235 schema:givenName Ruiqiang
    236 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016242423704.83
    237 rdf:type schema:Person
    238 sg:person.0601156616.50 schema:affiliation grid-institutes:grid.22072.35
    239 schema:familyName Burkinshaw
    240 schema:givenName Brianne
    241 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0601156616.50
    242 rdf:type schema:Person
    243 sg:person.0641126326.37 schema:affiliation grid-institutes:grid.262962.b
    244 schema:familyName Zhang
    245 schema:givenName Dapeng
    246 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0641126326.37
    247 rdf:type schema:Person
    248 sg:pub.10.1007/s00203-012-0816-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1026405140
    249 https://doi.org/10.1007/s00203-012-0816-z
    250 rdf:type schema:CreativeWork
    251 sg:pub.10.1038/386627a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036416155
    252 https://doi.org/10.1038/386627a0
    253 rdf:type schema:CreativeWork
    254 sg:pub.10.1038/nature10846 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015413547
    255 https://doi.org/10.1038/nature10846
    256 rdf:type schema:CreativeWork
    257 sg:pub.10.1038/nature12453 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050066013
    258 https://doi.org/10.1038/nature12453
    259 rdf:type schema:CreativeWork
    260 sg:pub.10.1038/nature12465 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025304440
    261 https://doi.org/10.1038/nature12465
    262 rdf:type schema:CreativeWork
    263 sg:pub.10.1038/nature13015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050163881
    264 https://doi.org/10.1038/nature13015
    265 rdf:type schema:CreativeWork
    266 sg:pub.10.1038/ncomms14888 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084129063
    267 https://doi.org/10.1038/ncomms14888
    268 rdf:type schema:CreativeWork
    269 sg:pub.10.1038/nprot.2015.053 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001304490
    270 https://doi.org/10.1038/nprot.2015.053
    271 rdf:type schema:CreativeWork
    272 sg:pub.10.1038/nrmicro2259 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036917778
    273 https://doi.org/10.1038/nrmicro2259
    274 rdf:type schema:CreativeWork
    275 sg:pub.10.1038/nrmicro3185 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024058313
    276 https://doi.org/10.1038/nrmicro3185
    277 rdf:type schema:CreativeWork
    278 sg:pub.10.1038/nrmicro3456 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043059122
    279 https://doi.org/10.1038/nrmicro3456
    280 rdf:type schema:CreativeWork
    281 sg:pub.10.1038/s41564-018-0144-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1103192448
    282 https://doi.org/10.1038/s41564-018-0144-4
    283 rdf:type schema:CreativeWork
    284 sg:pub.10.1186/1471-2105-5-113 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040413794
    285 https://doi.org/10.1186/1471-2105-5-113
    286 rdf:type schema:CreativeWork
    287 sg:pub.10.1186/1471-2105-6-298 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047546418
    288 https://doi.org/10.1186/1471-2105-6-298
    289 rdf:type schema:CreativeWork
    290 sg:pub.10.1186/1745-6150-7-18 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040385591
    291 https://doi.org/10.1186/1745-6150-7-18
    292 rdf:type schema:CreativeWork
    293 grid-institutes:grid.16821.3c schema:alternateName State Key Laboratory of Microbial Metabolism, Joint International Laboratory on Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240 China
    294 schema:name Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, AB T2N 4Z6 Canada
    295 Department of Ecosystem and Public Health, University of Calgary, Calgary, AB T2N 4Z6 Canada
    296 Snyder Institute for Chronic Diseases, University of Calgary, Calgary, AB T2N 4Z6 Canada
    297 State Key Laboratory of Microbial Metabolism, Joint International Laboratory on Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240 China
    298 rdf:type schema:Organization
    299 grid-institutes:grid.22072.35 schema:alternateName Snyder Institute for Chronic Diseases, University of Calgary, Calgary, AB T2N 4Z6 Canada
    300 schema:name Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, AB T2N 4Z6 Canada
    301 Department of Ecosystem and Public Health, University of Calgary, Calgary, AB T2N 4Z6 Canada
    302 Snyder Institute for Chronic Diseases, University of Calgary, Calgary, AB T2N 4Z6 Canada
    303 rdf:type schema:Organization
    304 grid-institutes:grid.262962.b schema:alternateName Department of Biology, College of Arts and Sciences, Saint Louis University, Saint Louis, MO 63103 USA
    305 Program of Bioinformatics and Computational Biology, College of Arts and Sciences, Saint Louis University, Saint Louis, MO 63103 USA
    306 schema:name Department of Biology, College of Arts and Sciences, Saint Louis University, Saint Louis, MO 63103 USA
    307 Program of Bioinformatics and Computational Biology, College of Arts and Sciences, Saint Louis University, Saint Louis, MO 63103 USA
    308 rdf:type schema:Organization
    309 grid-institutes:grid.419234.9 schema:alternateName National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894 USA
    310 schema:name National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894 USA
    311 rdf:type schema:Organization
    312 grid-institutes:grid.437123.0 schema:alternateName Faculty of Health Sciences, University of Macau, Macau SAR, China
    313 schema:name Faculty of Health Sciences, University of Macau, Macau SAR, China
    314 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...