Embryos and embryonic stem cells from the white rhinoceros View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-12

AUTHORS

Thomas B. Hildebrandt, Robert Hermes, Silvia Colleoni, Sebastian Diecke, Susanne Holtze, Marilyn B. Renfree, Jan Stejskal, Katsuhiko Hayashi, Micha Drukker, Pasqualino Loi, Frank Göritz, Giovanna Lazzari, Cesare Galli

ABSTRACT

The northern white rhinoceros (NWR, Ceratotherium simum cottoni) is the most endangered mammal in the world with only two females surviving. Here we adapt existing assisted reproduction techniques (ART) to fertilize Southern White Rhinoceros (SWR) oocytes with NWR spermatozoa. We show that rhinoceros oocytes can be repeatedly recovered from live SWR females by transrectal ovum pick-up, matured, fertilized by intracytoplasmic sperm injection and developed to the blastocyst stage in vitro. Next, we generate hybrid rhinoceros embryos in vitro using gametes of NWR and SWR. We also establish embryonic stem cell lines from the SWR blastocysts. Blastocysts are cryopreserved for later embryo transfer. Our results indicate that ART could be a viable strategy to rescue genes from the iconic, almost extinct, northern white rhinoceros and may also have broader impact if applied with similar success to other endangered large mammalian species. More... »

PAGES

2589

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41467-018-04959-2

DOI

http://dx.doi.org/10.1038/s41467-018-04959-2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1105180689

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29973581


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