Dynamic structural states of ClpB involved in its disaggregation function View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-12

AUTHORS

Takayuki Uchihashi, Yo-hei Watanabe, Yosuke Nakazaki, Takashi Yamasaki, Hiroki Watanabe, Takahiro Maruno, Kentaro Ishii, Susumu Uchiyama, Chihong Song, Kazuyoshi Murata, Ryota Iino, Toshio Ando

ABSTRACT

The ATP-dependent bacterial protein disaggregation machine, ClpB belonging to the AAA+ superfamily, refolds toxic protein aggregates into the native state in cooperation with the cognate Hsp70 partner. The ring-shaped hexamers of ClpB unfold and thread its protein substrate through the central pore. However, their function-related structural dynamics has remained elusive. Here we directly visualize ClpB using high-speed atomic force microscopy (HS-AFM) to gain a mechanistic insight into its disaggregation function. The HS-AFM movies demonstrate massive conformational changes of the hexameric ring during ATP hydrolysis, from a round ring to a spiral and even to a pair of twisted half-spirals. HS-AFM observations of Walker-motif mutants unveil crucial roles of ATP binding and hydrolysis in the oligomer formation and structural dynamics. Furthermore, repressed and hyperactive mutations result in significantly different oligomeric forms. These results provide a comprehensive view for the ATP-driven oligomeric-state transitions that enable ClpB to disentangle protein aggregates. More... »

PAGES

2147

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41467-018-04587-w

DOI

http://dx.doi.org/10.1038/s41467-018-04587-w

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1104243563

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29858573


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Nagoya University", 
          "id": "https://www.grid.ac/institutes/grid.27476.30", 
          "name": [
            "Department of Physics and Structural Biology Research Center, Nagoya University, 464-8602, Chikusa-ku, Nagoya, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Uchihashi", 
        "givenName": "Takayuki", 
        "id": "sg:person.01345655537.44", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01345655537.44"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Konan University", 
          "id": "https://www.grid.ac/institutes/grid.258669.6", 
          "name": [
            "Department of Biology, Faculty of Science and Engineering, Konan University, Okamoto 8-9-1, 658-8501, Kobe, Japan", 
            "Institute for Integrative Neurobiology, Konan University, Okamoto 8-9-1, 658-8501, Kobe, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Watanabe", 
        "givenName": "Yo-hei", 
        "id": "sg:person.01370145716.23", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01370145716.23"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Konan University", 
          "id": "https://www.grid.ac/institutes/grid.258669.6", 
          "name": [
            "Department of Biology, Faculty of Science and Engineering, Konan University, Okamoto 8-9-1, 658-8501, Kobe, Japan", 
            "Institute for Integrative Neurobiology, Konan University, Okamoto 8-9-1, 658-8501, Kobe, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nakazaki", 
        "givenName": "Yosuke", 
        "id": "sg:person.01034240066.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01034240066.05"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Konan University", 
          "id": "https://www.grid.ac/institutes/grid.258669.6", 
          "name": [
            "Department of Biology, Faculty of Science and Engineering, Konan University, Okamoto 8-9-1, 658-8501, Kobe, Japan", 
            "Institute for Integrative Neurobiology, Konan University, Okamoto 8-9-1, 658-8501, Kobe, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yamasaki", 
        "givenName": "Takashi", 
        "id": "sg:person.01207145520.19", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01207145520.19"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Kanazawa University", 
          "id": "https://www.grid.ac/institutes/grid.9707.9", 
          "name": [
            "Department of Physics, College of Science and Engineering, Kanazawa University, 920-1192, Kanazawa, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Watanabe", 
        "givenName": "Hiroki", 
        "id": "sg:person.01210561367.47", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01210561367.47"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Osaka University", 
          "id": "https://www.grid.ac/institutes/grid.136593.b", 
          "name": [
            "Department of Biotechnology, Graduate School of Engineering, Osaka University, 565-0871, Osaka, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Maruno", 
        "givenName": "Takahiro", 
        "id": "sg:person.01340271505.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01340271505.35"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institutes of Natural Sciences", 
          "id": "https://www.grid.ac/institutes/grid.250358.9", 
          "name": [
            "Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences, 444-8787, Okazaki, Aichi, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ishii", 
        "givenName": "Kentaro", 
        "id": "sg:person.011576054421.08", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011576054421.08"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institutes of Natural Sciences", 
          "id": "https://www.grid.ac/institutes/grid.250358.9", 
          "name": [
            "Department of Biotechnology, Graduate School of Engineering, Osaka University, 565-0871, Osaka, Japan", 
            "Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences, 444-8787, Okazaki, Aichi, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Uchiyama", 
        "givenName": "Susumu", 
        "id": "sg:person.01224523567.46", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01224523567.46"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute for Physiological Sciences", 
          "id": "https://www.grid.ac/institutes/grid.467811.d", 
          "name": [
            "National Institute for Physiological Sciences, National Institutes of Natural Sciences, 444-8787, Okazaki, Aichi, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Song", 
        "givenName": "Chihong", 
        "id": "sg:person.01327446741.58", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01327446741.58"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute for Physiological Sciences", 
          "id": "https://www.grid.ac/institutes/grid.467811.d", 
          "name": [
            "National Institute for Physiological Sciences, National Institutes of Natural Sciences, 444-8787, Okazaki, Aichi, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Murata", 
        "givenName": "Kazuyoshi", 
        "id": "sg:person.01333702112.14", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01333702112.14"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Graduate University For Advanced Studies", 
          "id": "https://www.grid.ac/institutes/grid.275033.0", 
          "name": [
            "Institute for Molecular Science, National Institutes of Natural Sciences, 444-8787, Okazaki, Aichi, Japan", 
            "Department of Functional Molecular Science, School of Physical Sciences, The Graduate University for Advanced Studies (SOKENDAI), 240-0193, Hayama, Kanagawa, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Iino", 
        "givenName": "Ryota", 
        "id": "sg:person.0737045434.41", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0737045434.41"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Kanazawa University", 
          "id": "https://www.grid.ac/institutes/grid.9707.9", 
          "name": [
            "Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, 920-1192, Kanazawa, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ando", 
        "givenName": "Toshio", 
        "id": "sg:person.01277542337.19", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01277542337.19"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1074/jbc.m303653200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000144245"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.7554/elife.02481", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000427062"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-2443.2001.00447.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000642581"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m114.617696", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001572286"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/16.15.7351", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002118380"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)81223-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002728727"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m209686200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003307928"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/346623a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003460696", 
          "https://doi.org/10.1038/346623a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0146-664x(82)90034-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003975878"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2443.2009.01357.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004662821"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2443.2009.01357.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004662821"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.96.13.7184", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005187345"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jmbi.2001.4455", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005537739"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m505653200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007125174"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2007.01.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008432174"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2006.11.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009277264"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m112.387589", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009309640"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m111.231365", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009931574"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1742-4658.2012.08540.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011018340"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0014-5793(97)00847-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011439208"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nprot.2012.047", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013400325", 
          "https://doi.org/10.1038/nprot.2012.047"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0006-3495(00)76713-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014356043"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.274.40.28083", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015023398"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/gtc.12188", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016376518"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m308782200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016911941"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1110/ps.03422604", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017272922"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2009.09.034", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019848903"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jsb.2012.09.006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019988217"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nsmb.2441", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021509154", 
          "https://doi.org/10.1038/nsmb.2441"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(89)90358-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021797759"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(89)90358-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021797759"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jmbi.2000.4165", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022882007"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.sbi.2013.01.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023257528"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.96.24.13732", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023510252"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.febslet.2010.01.029", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024921058"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gr.9.1.27", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028243295"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nsmb.3277", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028256551", 
          "https://doi.org/10.1038/nsmb.3277"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jsbi.1999.4174", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028456646"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj20082238", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028658655"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj20082238", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028658655"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj20082238", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028658655"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.271.29.17343", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028826396"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature09450", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030990737", 
          "https://doi.org/10.1038/nature09450"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature09450", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030990737", 
          "https://doi.org/10.1038/nature09450"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/cr4003837", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031289510"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m414623200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034434374"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jmb.2008.02.026", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035864233"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.str.2013.08.017", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036470450"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2004.11.027", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037520894"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(03)00807-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040352725"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nsmb.1425", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040563243", 
          "https://doi.org/10.1038/nsmb.1425"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1091/mbc.e02-08-0502", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042485248"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1233066", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042732304"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m109349200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043347096"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.7554/elife.21516", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046076205"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1205510", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046397104"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/srep18167", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047795237", 
          "https://doi.org/10.1038/srep18167"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi026161s", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055196012"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi026161s", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055196012"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.2188365", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062525954"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.272.5268.1606", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062553303"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082749798", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083320198", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.aan1052", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1086130320"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/sciadv.1701726", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1091054098"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/s41598-017-08917-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1091161885", 
          "https://doi.org/10.1038/s41598-017-08917-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/s41598-017-08917-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1091161885", 
          "https://doi.org/10.1038/s41598-017-08917-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/s41598-017-08917-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1091161885", 
          "https://doi.org/10.1038/s41598-017-08917-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbagen.2017.09.019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1091986707"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2018-12", 
    "datePublishedReg": "2018-12-01", 
    "description": "The ATP-dependent bacterial protein disaggregation machine, ClpB belonging to the AAA+ superfamily, refolds toxic protein aggregates into the native state in cooperation with the cognate Hsp70 partner. The ring-shaped hexamers of ClpB unfold and thread its protein substrate through the central pore. However, their function-related structural dynamics has remained elusive. Here we directly visualize ClpB using high-speed atomic force microscopy (HS-AFM) to gain a mechanistic insight into its disaggregation function. The HS-AFM movies demonstrate massive conformational changes of the hexameric ring during ATP hydrolysis, from a round ring to a spiral and even to a pair of twisted half-spirals. HS-AFM observations of Walker-motif mutants unveil crucial roles of ATP binding and hydrolysis in the oligomer formation and structural dynamics. Furthermore, repressed and hyperactive mutations result in significantly different oligomeric forms. These results provide a comprehensive view for the ATP-driven oligomeric-state transitions that enable ClpB to disentangle protein aggregates.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/s41467-018-04587-w", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.6821947", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.5900829", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.5856045", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.5897340", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.5900227", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.5899256", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.6958671", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.5902234", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.5846346", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1043282", 
        "issn": [
          "2041-1723"
        ], 
        "name": "Nature Communications", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "9"
      }
    ], 
    "name": "Dynamic structural states of ClpB involved in its disaggregation function", 
    "pagination": "2147", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "9f2c4d2441b55aa699760b4957073554eb1c6edcee7a224a7751649432ca6370"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "29858573"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101528555"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/s41467-018-04587-w"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1104243563"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/s41467-018-04587-w", 
      "https://app.dimensions.ai/details/publication/pub.1104243563"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T13:27", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8659_00000567.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/s41467-018-04587-w"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41467-018-04587-w'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41467-018-04587-w'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41467-018-04587-w'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41467-018-04587-w'


 

This table displays all metadata directly associated to this object as RDF triples.

374 TRIPLES      21 PREDICATES      90 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/s41467-018-04587-w schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author Ne90dde506a424330902d2e50048c1769
4 schema:citation sg:pub.10.1038/346623a0
5 sg:pub.10.1038/nature09450
6 sg:pub.10.1038/nprot.2012.047
7 sg:pub.10.1038/nsmb.1425
8 sg:pub.10.1038/nsmb.2441
9 sg:pub.10.1038/nsmb.3277
10 sg:pub.10.1038/s41598-017-08917-8
11 sg:pub.10.1038/srep18167
12 https://app.dimensions.ai/details/publication/pub.1082749798
13 https://app.dimensions.ai/details/publication/pub.1083320198
14 https://doi.org/10.1006/jmbi.2000.4165
15 https://doi.org/10.1006/jmbi.2001.4455
16 https://doi.org/10.1006/jsbi.1999.4174
17 https://doi.org/10.1016/0146-664x(82)90034-x
18 https://doi.org/10.1016/0378-1119(89)90358-2
19 https://doi.org/10.1016/j.bbagen.2017.09.019
20 https://doi.org/10.1016/j.cell.2004.11.027
21 https://doi.org/10.1016/j.cell.2009.09.034
22 https://doi.org/10.1016/j.febslet.2010.01.029
23 https://doi.org/10.1016/j.jmb.2008.02.026
24 https://doi.org/10.1016/j.jsb.2012.09.006
25 https://doi.org/10.1016/j.molcel.2006.11.008
26 https://doi.org/10.1016/j.molcel.2007.01.002
27 https://doi.org/10.1016/j.sbi.2013.01.004
28 https://doi.org/10.1016/j.str.2013.08.017
29 https://doi.org/10.1016/s0006-3495(00)76713-0
30 https://doi.org/10.1016/s0014-5793(97)00847-8
31 https://doi.org/10.1016/s0092-8674(00)81223-4
32 https://doi.org/10.1016/s0092-8674(03)00807-9
33 https://doi.org/10.1021/bi026161s
34 https://doi.org/10.1021/cr4003837
35 https://doi.org/10.1042/bj20082238
36 https://doi.org/10.1046/j.1365-2443.2001.00447.x
37 https://doi.org/10.1073/pnas.96.13.7184
38 https://doi.org/10.1073/pnas.96.24.13732
39 https://doi.org/10.1074/jbc.271.29.17343
40 https://doi.org/10.1074/jbc.274.40.28083
41 https://doi.org/10.1074/jbc.m109349200
42 https://doi.org/10.1074/jbc.m111.231365
43 https://doi.org/10.1074/jbc.m112.387589
44 https://doi.org/10.1074/jbc.m114.617696
45 https://doi.org/10.1074/jbc.m209686200
46 https://doi.org/10.1074/jbc.m303653200
47 https://doi.org/10.1074/jbc.m308782200
48 https://doi.org/10.1074/jbc.m414623200
49 https://doi.org/10.1074/jbc.m505653200
50 https://doi.org/10.1091/mbc.e02-08-0502
51 https://doi.org/10.1093/nar/16.15.7351
52 https://doi.org/10.1101/gr.9.1.27
53 https://doi.org/10.1110/ps.03422604
54 https://doi.org/10.1111/gtc.12188
55 https://doi.org/10.1111/j.1365-2443.2009.01357.x
56 https://doi.org/10.1111/j.1742-4658.2012.08540.x
57 https://doi.org/10.1126/sciadv.1701726
58 https://doi.org/10.1126/science.1205510
59 https://doi.org/10.1126/science.1233066
60 https://doi.org/10.1126/science.2188365
61 https://doi.org/10.1126/science.272.5268.1606
62 https://doi.org/10.1126/science.aan1052
63 https://doi.org/10.7554/elife.02481
64 https://doi.org/10.7554/elife.21516
65 schema:datePublished 2018-12
66 schema:datePublishedReg 2018-12-01
67 schema:description The ATP-dependent bacterial protein disaggregation machine, ClpB belonging to the AAA+ superfamily, refolds toxic protein aggregates into the native state in cooperation with the cognate Hsp70 partner. The ring-shaped hexamers of ClpB unfold and thread its protein substrate through the central pore. However, their function-related structural dynamics has remained elusive. Here we directly visualize ClpB using high-speed atomic force microscopy (HS-AFM) to gain a mechanistic insight into its disaggregation function. The HS-AFM movies demonstrate massive conformational changes of the hexameric ring during ATP hydrolysis, from a round ring to a spiral and even to a pair of twisted half-spirals. HS-AFM observations of Walker-motif mutants unveil crucial roles of ATP binding and hydrolysis in the oligomer formation and structural dynamics. Furthermore, repressed and hyperactive mutations result in significantly different oligomeric forms. These results provide a comprehensive view for the ATP-driven oligomeric-state transitions that enable ClpB to disentangle protein aggregates.
68 schema:genre research_article
69 schema:inLanguage en
70 schema:isAccessibleForFree true
71 schema:isPartOf N7d2624a0e39e400a9cc30375b2bab9bd
72 Nb057b114cf044e8495e1754f3c6410a4
73 sg:journal.1043282
74 schema:name Dynamic structural states of ClpB involved in its disaggregation function
75 schema:pagination 2147
76 schema:productId N5d785172deb34ff0a24828bf9a5a0037
77 N5fe0cec0e8f44420aa2b34e9ad32c693
78 N63354950409744508709871306f82b19
79 Naa5dcc8ad29e4252934b8291f4f7dc5f
80 Nd27304d931944a448464eb957d05b93b
81 schema:sameAs https://app.dimensions.ai/details/publication/pub.1104243563
82 https://doi.org/10.1038/s41467-018-04587-w
83 schema:sdDatePublished 2019-04-10T13:27
84 schema:sdLicense https://scigraph.springernature.com/explorer/license/
85 schema:sdPublisher N92d534f80a004830bf0cc87d386daaaf
86 schema:url https://www.nature.com/articles/s41467-018-04587-w
87 sgo:license sg:explorer/license/
88 sgo:sdDataset articles
89 rdf:type schema:ScholarlyArticle
90 N1dab33c41bd449119b3cdef9512436c7 rdf:first sg:person.01277542337.19
91 rdf:rest rdf:nil
92 N23131c06834147e09aadd72c5f03c2a9 rdf:first sg:person.011576054421.08
93 rdf:rest N8bd1986f7fa8488f8e879852cf338a80
94 N2a5f8e424dfe48b7aab15175e9d74806 rdf:first sg:person.01327446741.58
95 rdf:rest Nd4494d8e5ef9448194743287da8f3e94
96 N3705bb2b1a0e41718ea68455456597a7 rdf:first sg:person.01034240066.05
97 rdf:rest Ne16a3caeb23a49fa80516b506cf4a872
98 N5776b4b41bea4feea3343d1fd2a87336 rdf:first sg:person.01340271505.35
99 rdf:rest N23131c06834147e09aadd72c5f03c2a9
100 N5d785172deb34ff0a24828bf9a5a0037 schema:name pubmed_id
101 schema:value 29858573
102 rdf:type schema:PropertyValue
103 N5fe0cec0e8f44420aa2b34e9ad32c693 schema:name doi
104 schema:value 10.1038/s41467-018-04587-w
105 rdf:type schema:PropertyValue
106 N63354950409744508709871306f82b19 schema:name dimensions_id
107 schema:value pub.1104243563
108 rdf:type schema:PropertyValue
109 N7d2624a0e39e400a9cc30375b2bab9bd schema:issueNumber 1
110 rdf:type schema:PublicationIssue
111 N8bd1986f7fa8488f8e879852cf338a80 rdf:first sg:person.01224523567.46
112 rdf:rest N2a5f8e424dfe48b7aab15175e9d74806
113 N8db8ae2b2e954a66a8b2fb5472866d23 rdf:first sg:person.01210561367.47
114 rdf:rest N5776b4b41bea4feea3343d1fd2a87336
115 N92d534f80a004830bf0cc87d386daaaf schema:name Springer Nature - SN SciGraph project
116 rdf:type schema:Organization
117 Naa5dcc8ad29e4252934b8291f4f7dc5f schema:name nlm_unique_id
118 schema:value 101528555
119 rdf:type schema:PropertyValue
120 Nb057b114cf044e8495e1754f3c6410a4 schema:volumeNumber 9
121 rdf:type schema:PublicationVolume
122 Nd27304d931944a448464eb957d05b93b schema:name readcube_id
123 schema:value 9f2c4d2441b55aa699760b4957073554eb1c6edcee7a224a7751649432ca6370
124 rdf:type schema:PropertyValue
125 Nd4494d8e5ef9448194743287da8f3e94 rdf:first sg:person.01333702112.14
126 rdf:rest Nd53c6636038f49c1bb3315f870e5ae17
127 Nd53c6636038f49c1bb3315f870e5ae17 rdf:first sg:person.0737045434.41
128 rdf:rest N1dab33c41bd449119b3cdef9512436c7
129 Nda6e9131f1c94c34953ec53126e0792d rdf:first sg:person.01370145716.23
130 rdf:rest N3705bb2b1a0e41718ea68455456597a7
131 Ne16a3caeb23a49fa80516b506cf4a872 rdf:first sg:person.01207145520.19
132 rdf:rest N8db8ae2b2e954a66a8b2fb5472866d23
133 Ne90dde506a424330902d2e50048c1769 rdf:first sg:person.01345655537.44
134 rdf:rest Nda6e9131f1c94c34953ec53126e0792d
135 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
136 schema:name Biological Sciences
137 rdf:type schema:DefinedTerm
138 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
139 schema:name Biochemistry and Cell Biology
140 rdf:type schema:DefinedTerm
141 sg:grant.5846346 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-018-04587-w
142 rdf:type schema:MonetaryGrant
143 sg:grant.5856045 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-018-04587-w
144 rdf:type schema:MonetaryGrant
145 sg:grant.5897340 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-018-04587-w
146 rdf:type schema:MonetaryGrant
147 sg:grant.5899256 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-018-04587-w
148 rdf:type schema:MonetaryGrant
149 sg:grant.5900227 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-018-04587-w
150 rdf:type schema:MonetaryGrant
151 sg:grant.5900829 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-018-04587-w
152 rdf:type schema:MonetaryGrant
153 sg:grant.5902234 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-018-04587-w
154 rdf:type schema:MonetaryGrant
155 sg:grant.6821947 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-018-04587-w
156 rdf:type schema:MonetaryGrant
157 sg:grant.6958671 http://pending.schema.org/fundedItem sg:pub.10.1038/s41467-018-04587-w
158 rdf:type schema:MonetaryGrant
159 sg:journal.1043282 schema:issn 2041-1723
160 schema:name Nature Communications
161 rdf:type schema:Periodical
162 sg:person.01034240066.05 schema:affiliation https://www.grid.ac/institutes/grid.258669.6
163 schema:familyName Nakazaki
164 schema:givenName Yosuke
165 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01034240066.05
166 rdf:type schema:Person
167 sg:person.011576054421.08 schema:affiliation https://www.grid.ac/institutes/grid.250358.9
168 schema:familyName Ishii
169 schema:givenName Kentaro
170 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011576054421.08
171 rdf:type schema:Person
172 sg:person.01207145520.19 schema:affiliation https://www.grid.ac/institutes/grid.258669.6
173 schema:familyName Yamasaki
174 schema:givenName Takashi
175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01207145520.19
176 rdf:type schema:Person
177 sg:person.01210561367.47 schema:affiliation https://www.grid.ac/institutes/grid.9707.9
178 schema:familyName Watanabe
179 schema:givenName Hiroki
180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01210561367.47
181 rdf:type schema:Person
182 sg:person.01224523567.46 schema:affiliation https://www.grid.ac/institutes/grid.250358.9
183 schema:familyName Uchiyama
184 schema:givenName Susumu
185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01224523567.46
186 rdf:type schema:Person
187 sg:person.01277542337.19 schema:affiliation https://www.grid.ac/institutes/grid.9707.9
188 schema:familyName Ando
189 schema:givenName Toshio
190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01277542337.19
191 rdf:type schema:Person
192 sg:person.01327446741.58 schema:affiliation https://www.grid.ac/institutes/grid.467811.d
193 schema:familyName Song
194 schema:givenName Chihong
195 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01327446741.58
196 rdf:type schema:Person
197 sg:person.01333702112.14 schema:affiliation https://www.grid.ac/institutes/grid.467811.d
198 schema:familyName Murata
199 schema:givenName Kazuyoshi
200 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01333702112.14
201 rdf:type schema:Person
202 sg:person.01340271505.35 schema:affiliation https://www.grid.ac/institutes/grid.136593.b
203 schema:familyName Maruno
204 schema:givenName Takahiro
205 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01340271505.35
206 rdf:type schema:Person
207 sg:person.01345655537.44 schema:affiliation https://www.grid.ac/institutes/grid.27476.30
208 schema:familyName Uchihashi
209 schema:givenName Takayuki
210 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01345655537.44
211 rdf:type schema:Person
212 sg:person.01370145716.23 schema:affiliation https://www.grid.ac/institutes/grid.258669.6
213 schema:familyName Watanabe
214 schema:givenName Yo-hei
215 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01370145716.23
216 rdf:type schema:Person
217 sg:person.0737045434.41 schema:affiliation https://www.grid.ac/institutes/grid.275033.0
218 schema:familyName Iino
219 schema:givenName Ryota
220 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0737045434.41
221 rdf:type schema:Person
222 sg:pub.10.1038/346623a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003460696
223 https://doi.org/10.1038/346623a0
224 rdf:type schema:CreativeWork
225 sg:pub.10.1038/nature09450 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030990737
226 https://doi.org/10.1038/nature09450
227 rdf:type schema:CreativeWork
228 sg:pub.10.1038/nprot.2012.047 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013400325
229 https://doi.org/10.1038/nprot.2012.047
230 rdf:type schema:CreativeWork
231 sg:pub.10.1038/nsmb.1425 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040563243
232 https://doi.org/10.1038/nsmb.1425
233 rdf:type schema:CreativeWork
234 sg:pub.10.1038/nsmb.2441 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021509154
235 https://doi.org/10.1038/nsmb.2441
236 rdf:type schema:CreativeWork
237 sg:pub.10.1038/nsmb.3277 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028256551
238 https://doi.org/10.1038/nsmb.3277
239 rdf:type schema:CreativeWork
240 sg:pub.10.1038/s41598-017-08917-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1091161885
241 https://doi.org/10.1038/s41598-017-08917-8
242 rdf:type schema:CreativeWork
243 sg:pub.10.1038/srep18167 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047795237
244 https://doi.org/10.1038/srep18167
245 rdf:type schema:CreativeWork
246 https://app.dimensions.ai/details/publication/pub.1082749798 schema:CreativeWork
247 https://app.dimensions.ai/details/publication/pub.1083320198 schema:CreativeWork
248 https://doi.org/10.1006/jmbi.2000.4165 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022882007
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1006/jmbi.2001.4455 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005537739
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1006/jsbi.1999.4174 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028456646
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1016/0146-664x(82)90034-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1003975878
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1016/0378-1119(89)90358-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021797759
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1016/j.bbagen.2017.09.019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1091986707
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1016/j.cell.2004.11.027 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037520894
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1016/j.cell.2009.09.034 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019848903
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1016/j.febslet.2010.01.029 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024921058
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1016/j.jmb.2008.02.026 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035864233
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1016/j.jsb.2012.09.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019988217
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1016/j.molcel.2006.11.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009277264
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1016/j.molcel.2007.01.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008432174
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1016/j.sbi.2013.01.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023257528
275 rdf:type schema:CreativeWork
276 https://doi.org/10.1016/j.str.2013.08.017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036470450
277 rdf:type schema:CreativeWork
278 https://doi.org/10.1016/s0006-3495(00)76713-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014356043
279 rdf:type schema:CreativeWork
280 https://doi.org/10.1016/s0014-5793(97)00847-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011439208
281 rdf:type schema:CreativeWork
282 https://doi.org/10.1016/s0092-8674(00)81223-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002728727
283 rdf:type schema:CreativeWork
284 https://doi.org/10.1016/s0092-8674(03)00807-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040352725
285 rdf:type schema:CreativeWork
286 https://doi.org/10.1021/bi026161s schema:sameAs https://app.dimensions.ai/details/publication/pub.1055196012
287 rdf:type schema:CreativeWork
288 https://doi.org/10.1021/cr4003837 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031289510
289 rdf:type schema:CreativeWork
290 https://doi.org/10.1042/bj20082238 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028658655
291 rdf:type schema:CreativeWork
292 https://doi.org/10.1046/j.1365-2443.2001.00447.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1000642581
293 rdf:type schema:CreativeWork
294 https://doi.org/10.1073/pnas.96.13.7184 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005187345
295 rdf:type schema:CreativeWork
296 https://doi.org/10.1073/pnas.96.24.13732 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023510252
297 rdf:type schema:CreativeWork
298 https://doi.org/10.1074/jbc.271.29.17343 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028826396
299 rdf:type schema:CreativeWork
300 https://doi.org/10.1074/jbc.274.40.28083 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015023398
301 rdf:type schema:CreativeWork
302 https://doi.org/10.1074/jbc.m109349200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043347096
303 rdf:type schema:CreativeWork
304 https://doi.org/10.1074/jbc.m111.231365 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009931574
305 rdf:type schema:CreativeWork
306 https://doi.org/10.1074/jbc.m112.387589 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009309640
307 rdf:type schema:CreativeWork
308 https://doi.org/10.1074/jbc.m114.617696 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001572286
309 rdf:type schema:CreativeWork
310 https://doi.org/10.1074/jbc.m209686200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003307928
311 rdf:type schema:CreativeWork
312 https://doi.org/10.1074/jbc.m303653200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000144245
313 rdf:type schema:CreativeWork
314 https://doi.org/10.1074/jbc.m308782200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016911941
315 rdf:type schema:CreativeWork
316 https://doi.org/10.1074/jbc.m414623200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034434374
317 rdf:type schema:CreativeWork
318 https://doi.org/10.1074/jbc.m505653200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007125174
319 rdf:type schema:CreativeWork
320 https://doi.org/10.1091/mbc.e02-08-0502 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042485248
321 rdf:type schema:CreativeWork
322 https://doi.org/10.1093/nar/16.15.7351 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002118380
323 rdf:type schema:CreativeWork
324 https://doi.org/10.1101/gr.9.1.27 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028243295
325 rdf:type schema:CreativeWork
326 https://doi.org/10.1110/ps.03422604 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017272922
327 rdf:type schema:CreativeWork
328 https://doi.org/10.1111/gtc.12188 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016376518
329 rdf:type schema:CreativeWork
330 https://doi.org/10.1111/j.1365-2443.2009.01357.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1004662821
331 rdf:type schema:CreativeWork
332 https://doi.org/10.1111/j.1742-4658.2012.08540.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1011018340
333 rdf:type schema:CreativeWork
334 https://doi.org/10.1126/sciadv.1701726 schema:sameAs https://app.dimensions.ai/details/publication/pub.1091054098
335 rdf:type schema:CreativeWork
336 https://doi.org/10.1126/science.1205510 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046397104
337 rdf:type schema:CreativeWork
338 https://doi.org/10.1126/science.1233066 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042732304
339 rdf:type schema:CreativeWork
340 https://doi.org/10.1126/science.2188365 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062525954
341 rdf:type schema:CreativeWork
342 https://doi.org/10.1126/science.272.5268.1606 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062553303
343 rdf:type schema:CreativeWork
344 https://doi.org/10.1126/science.aan1052 schema:sameAs https://app.dimensions.ai/details/publication/pub.1086130320
345 rdf:type schema:CreativeWork
346 https://doi.org/10.7554/elife.02481 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000427062
347 rdf:type schema:CreativeWork
348 https://doi.org/10.7554/elife.21516 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046076205
349 rdf:type schema:CreativeWork
350 https://www.grid.ac/institutes/grid.136593.b schema:alternateName Osaka University
351 schema:name Department of Biotechnology, Graduate School of Engineering, Osaka University, 565-0871, Osaka, Japan
352 rdf:type schema:Organization
353 https://www.grid.ac/institutes/grid.250358.9 schema:alternateName National Institutes of Natural Sciences
354 schema:name Department of Biotechnology, Graduate School of Engineering, Osaka University, 565-0871, Osaka, Japan
355 Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences, 444-8787, Okazaki, Aichi, Japan
356 rdf:type schema:Organization
357 https://www.grid.ac/institutes/grid.258669.6 schema:alternateName Konan University
358 schema:name Department of Biology, Faculty of Science and Engineering, Konan University, Okamoto 8-9-1, 658-8501, Kobe, Japan
359 Institute for Integrative Neurobiology, Konan University, Okamoto 8-9-1, 658-8501, Kobe, Japan
360 rdf:type schema:Organization
361 https://www.grid.ac/institutes/grid.27476.30 schema:alternateName Nagoya University
362 schema:name Department of Physics and Structural Biology Research Center, Nagoya University, 464-8602, Chikusa-ku, Nagoya, Japan
363 rdf:type schema:Organization
364 https://www.grid.ac/institutes/grid.275033.0 schema:alternateName Graduate University For Advanced Studies
365 schema:name Department of Functional Molecular Science, School of Physical Sciences, The Graduate University for Advanced Studies (SOKENDAI), 240-0193, Hayama, Kanagawa, Japan
366 Institute for Molecular Science, National Institutes of Natural Sciences, 444-8787, Okazaki, Aichi, Japan
367 rdf:type schema:Organization
368 https://www.grid.ac/institutes/grid.467811.d schema:alternateName National Institute for Physiological Sciences
369 schema:name National Institute for Physiological Sciences, National Institutes of Natural Sciences, 444-8787, Okazaki, Aichi, Japan
370 rdf:type schema:Organization
371 https://www.grid.ac/institutes/grid.9707.9 schema:alternateName Kanazawa University
372 schema:name Department of Physics, College of Science and Engineering, Kanazawa University, 920-1192, Kanazawa, Japan
373 Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, 920-1192, Kanazawa, Japan
374 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...